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Sherlock Shrooms
Neophyte



Registered: 06/25/19
Posts: 606
Loc: Under the Sun
Last seen: 2 years, 10 months
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I'm wondering if I should roll the dice and assume it was an odd jar or 2 of bad spawn and just do like a 2:1 spawn to sub ratio in the rest of the tubs and cross my fingers
-------------------- "When you pass through the waters, I will be with you; And through the rivers, they shall not overflow you. When you walk through the fire, you shall not be burned, Nor shall the flame scorch you."

Edited by Sherlock Shrooms (07/09/19 09:30 PM)
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Crackatoa
Stranger in a strange land



Registered: 03/31/19
Posts: 5,399
Loc: Over by your Mama's house
Last seen: 2 hours, 10 minutes
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Were they all inoculated from the same source?
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R.I.P.Zappa
Myco Melyco


Registered: 03/30/18
Posts: 1,212
Loc: In Between Space and Time
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Re: Cultivation General Discussion [Re: Crackatoa]
#26099353 - 07/09/19 10:38 PM (4 years, 6 months ago) |
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Not really seeing anything uniform or organized on that mycellium at all. Even the splotches growing on the glass that give ya an ok 2d look at it on top are light and wispy looking.
If it were me I wouldn't feel comfortable spawning it because it just doesn't look right to me.
I would wait for more to chime in on it, better to hold on a day or more than waste a couple weeks and sub.
-------------------- -The heaviest thing one will ever carry is a thought- -"Like a Blind man In an orgy you gotta feel things out.".- -When we agree about our hallucinations, we call it “reality".- -If you defy authority because your told to, that's no better than blindly trusting authority.- psychonautwiki.org How it should & shouldn't look - NEW CULTIVATORS GUIDE BOD's Easy AF OAT prep tek. Principles of mushroom growing for beginners
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Boogieman47
Let's boogie


Registered: 03/05/16
Posts: 9,712
Loc: Under your bed
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Re: Cultivation General Discussion [Re: R.I.P.Zappa]
#26099367 - 07/09/19 10:49 PM (4 years, 6 months ago) |
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Yeah that Jars fucked possibly some type if bacteria possibly bacillus
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Legend21
Legend



Registered: 04/27/18
Posts: 1,148
Last seen: 1 year, 5 months
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Re: Cultivation General Discussion [Re: Boogieman47]
#26099613 - 07/10/19 04:47 AM (4 years, 6 months ago) |
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Found freaking MAGGOTS in my First ever Galindoi tray. So freaking gross. This is also my first time using Hpoo but I pasteurized at 140-160 for an hour. You Guys think it came from the hpoo? Maybe the rye? Or something after spawning.
My skins crawling I threw it out just trying to figure out where that came from.
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Changa Alchemist
Shwift sauce lifeguard



Registered: 03/16/16
Posts: 2,707
Loc: 90+% Rh
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Re: Cultivation General Discussion [Re: Legend21]
#26099641 - 07/10/19 05:38 AM (4 years, 6 months ago) |
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Yeah that's gross. Did you have gnats as well?
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hypercubeididnt
Stranger
Registered: 07/08/19
Posts: 11
Last seen: 3 years, 8 months
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hi ive grown some spores into mycelium in a grow box i have now placed the mycelium filled grow box into a humidity tent(a plastic bag) with damp perlite
i have a question regarding misting, do i mist/spray the top of the mycelium or the perlite around the sides/underneath the grow box?
and how many sprays/squirts should i give every time?
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icetech



Registered: 08/21/17
Posts: 3,450
Loc: FSM's loving noodles.
Last seen: 3 months, 5 days
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Re: Cultivation General Discussion [Re: Legend21]
#26099767 - 07/10/19 07:17 AM (4 years, 6 months ago) |
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Quote:
Legend21 said: Found freaking MAGGOTS in my First ever Galindoi tray. So freaking gross. This is also my first time using Hpoo but I pasteurized at 140-160 for an hour. You Guys think it came from the hpoo? Maybe the rye? Or something after spawning.
My skins crawling I threw it out just trying to figure out where that came from.
Just curious, does hpoo need to be pasteurized and not sterilized? or did you not have a way to sterilize? I have never used it and was just wondering...
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FiatFirmamentum
Stranger


Registered: 04/15/19
Posts: 59
Loc: Central EU
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Quote:
Sherlock Shrooms said: We are not doing professional sterilization in PC's. A more accurate term would be minimization. Bacterial endospores survive even at 20 psi for 3 hours. This is why you should use a large amount of inoculant so that cube myc can completely colonize the jar in as short a time as possible, if 3 weeks pass before the grains are fully colonized you may run into bacterial issues. They were there all along. So the higher the endospore count to begin with the more you may end up with at the end
Geobacillus stearothermophilus is the most heat-resistant bacteria known to men. And yet, 121*C at 15 psi for 12 minutes kill all of its endospores (Medical Microbiology (Eighteenth Edition), Churchill Livingstone, 2012, Pages 737-778). If you have contamination, you didn't achieve 121*C in some parts of grain or introduced contamination after sterillization.`
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Bumholio
What's the craic



Registered: 07/23/18
Posts: 4,269
Loc: Shroomsville
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Quote:
hypercubeididnt said: hi ive grown some spores into mycelium in a grow box i have now placed the mycelium filled grow box into a humidity tent(a plastic bag) with damp perlite
i have a question regarding misting, do i mist/spray the top of the mycelium or the perlite around the sides/underneath the grow box?
and how many sprays/squirts should i give every time?
How did you grow your spores in to mycelium, and what substrate are you using in your grow box?
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 "great things may come to those who wait, but only what's left by those who hustle"
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hypercubeididnt
Stranger
Registered: 07/08/19
Posts: 11
Last seen: 3 years, 8 months
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Re: Cultivation General Discussion [Re: Bumholio]
#26099838 - 07/10/19 09:32 AM (4 years, 6 months ago) |
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i just injected some spores into the special grow box(part of a kit including the plastic bag and perlite for fruiting) the substrates are Organic Rye Grain Substrate with added Coir, Gypsum and Coffee
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ShaperDreaming
Weirdo



Registered: 10/30/18
Posts: 3,429
Loc: United States
Last seen: 2 years, 3 days
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Re: Cultivation General Discussion [Re: mushboy]
#26099947 - 07/10/19 10:13 AM (4 years, 6 months ago) |
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Quote:
mushboy said: ive left grain jars for months. they just dry out before molding ime.
I wouldn't say months, but yeah. I wouldn't feel comfortable using a jar for more than a week or so due to loss of hydration. Anything you noc'd up in a jar that much later is going to be slow going.
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Legend21
Legend



Registered: 04/27/18
Posts: 1,148
Last seen: 1 year, 5 months
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Re: Cultivation General Discussion [Re: icetech]
#26099968 - 07/10/19 10:20 AM (4 years, 6 months ago) |
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Quote:
icetech said:
Quote:
Legend21 said: Found freaking MAGGOTS in my First ever Galindoi tray. So freaking gross. This is also my first time using Hpoo but I pasteurized at 140-160 for an hour. You Guys think it came from the hpoo? Maybe the rye? Or something after spawning.
My skins crawling I threw it out just trying to figure out where that came from.
Just curious, does hpoo need to be pasteurized and not sterilized? or did you not have a way to sterilize? I have never used it and was just wondering...
Yeah as far as I understand pretty much any substrate except coir needs to be pasteurized not sterilized. The only way I think you can sterilize manure is if your substrate is colonizing or fruiting in vitro I believe. Either way though you’d think 160 would kill maggots. It’s probably fungus gnat larvae
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ShaperDreaming
Weirdo



Registered: 10/30/18
Posts: 3,429
Loc: United States
Last seen: 2 years, 3 days
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Quote:
Legend21 said: Found freaking MAGGOTS in my First ever Galindoi tray

Once I got some wild hen of the woods. I was so stoked to get these fuckers home and cook them up... when I started chopping them up all these fucking worms started writhing from the center of my cuts and flopping out onto the cutting board... I ran retching to the toilet and didn't throw up, but still. OMG my skin is crawling just thinking about this. Nope! NOPE! Nope!
Quote:
Sherlock Shrooms said: I can't see any wetspot.
Wet spots usually indicate bacteria. Mold is super hard to spot pre-sporing. Sometimes you can just see different sectors. If it's meshed in... Super hard to tell.
Have you read these threads? They're super helpful when trying to ID your contaminations and also just knowing what to look for in general:
Recognizing and dealing with contamination How it should & shouldn't look - NEW CULTIVATORS GUIDE
Quote:
Legend21 said: Yeah as far as I understand pretty much any substrate except coir needs to be pasteurized not sterilized. The only way I think you can sterilize manure is if your substrate is colonizing or fruiting in vitro I believe.
Not exactly true. It just isn't recommended to sterlize them since that makes the sub much much much harder to work with. You have to work in a sterile environment everytime as it's far more likely to contaminate out.
Here's my TLDR for the difference: Pasteurizing doesn't kill all the helpful organism in the substrate, sterilization does. When those helpful organisms are dead lots of bad shit wants to get in there and take over. We want our mycelium to take over, and it can work with the good/helpful organisms, but the bad shit just wants it to turn green and will fight your myc and likely fuck up your fruits.
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hexkrak
VisualDecintigrator


Registered: 12/26/02
Posts: 270
Last seen: 4 years, 5 months
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Quote:
ShaperDreaming said:
Quote:
mushboy said: ive left grain jars for months. they just dry out before molding ime.
I wouldn't say months, but yeah. I wouldn't feel comfortable using a jar for more than a week or so due to loss of hydration. Anything you noc'd up in a jar that much later is going to be slow going.
I've gone 6+ weeks before with no noticeable downside.
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Always forgive your enemies; nothing annoys them more. --Oscar Wilde
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ShaperDreaming
Weirdo



Registered: 10/30/18
Posts: 3,429
Loc: United States
Last seen: 2 years, 3 days
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Re: Cultivation General Discussion [Re: hexkrak]
#26100004 - 07/10/19 10:31 AM (4 years, 6 months ago) |
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Hey, I ain't knocking it any, I'm just saying I wouldn't feel comfortable.
Like, sure I could eat that pb&j sandwich that's been sitting on the table for a few days that looks and smells good... but I don't want to.
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Legend21
Legend



Registered: 04/27/18
Posts: 1,148
Last seen: 1 year, 5 months
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Quote:
ShaperDreaming said:

Once I got some wild hen of the woods. I was so stoked to get these fuckers home and cook them up... when I started chopping them up all these fucking worms started writhing from the center of my cuts and flopping out onto the cutting board... I ran retching to the toilet and didn't throw up, but still. OMG my skin is crawling just thinking about this. Nope! NOPE! Nope!
Quote:
Legend21 said: Yeah as far as I understand pretty much any substrate except coir needs to be pasteurized not sterilized. The only way I think you can sterilize manure is if your substrate is colonizing or fruiting in vitro I believe.
Not exactly true. It just isn't recommended to sterlize them since that makes the sub much much much harder to work with. You have to work in a sterile environment everytime as it's far more likely to contaminate out.
Here's my TLDR for the difference: Pasteurizing doesn't kill all the helpful organism in the substrate, sterilization does. When those helpful organisms are dead lots of bad shit wants to get in there and take over. We want our mycelium to take over, and it can work with the good/helpful organisms, but the bad shit just wants it to turn green and will fight your myc and likely fuck up your fruits.
Yeah so then you would have to spawn in a SAB or infront of a flowhood wouldnt you? and even then it would have to colonize in a sterile environment correct? Either way I wouldn't sterilize manure since i spawn to bulk in open air. But I do want to know where these nasty shits came from.
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ShaperDreaming
Weirdo



Registered: 10/30/18
Posts: 3,429
Loc: United States
Last seen: 2 years, 3 days
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Re: Cultivation General Discussion [Re: Legend21]
#26100057 - 07/10/19 10:50 AM (4 years, 6 months ago) |
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Quote:
Legend21 said: Yeah so then you would have to spawn in a SAB or infront of a flowhood wouldnt you? and even then it would have to colonize in a sterile environment correct? Either way I wouldn't sterilize manure since i spawn to bulk in open air. But I do want to know where these nasty shits came from.
Exactly. Honestly I'm just starting to fuck around with sterilized subs and JESUS is this shit a pain in the ass to work with.
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FiatFirmamentum
Stranger


Registered: 04/15/19
Posts: 59
Loc: Central EU
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For edible species, manure and compost contain bacteria that stimulate pinning. If your species require hpoo, it's likely the same and it wouldn't fruit from sterilised hpoo.
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Legend21
Legend



Registered: 04/27/18
Posts: 1,148
Last seen: 1 year, 5 months
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Yeah I’m assuming that fungus gnats or some other fly laid eggs in it after I spawned. Problem is I have two. One I already cased and one I saw the maggots in. I’m assuming they could be in the other one as well but I can see them. If I don’t get pins in another week or so I’m tossing that one also.
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