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shroomator
Stranger

Registered: 06/17/19
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Re: Cultivation General Discussion [Re: Leftfield420]
#26095598 - 07/08/19 03:43 AM (4 years, 6 months ago) |
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How much mycelium do I transfer over with grain to grain?
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Leftfield420
bong toker



Registered: 02/26/16
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Re: Cultivation General Discussion [Re: shroomator]
#26095655 - 07/08/19 05:29 AM (4 years, 6 months ago) |
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If you're going grain to grain, you can turn 1qt colonized grain into 10qts...
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filthyknees
no coincidence


Registered: 03/08/13
Posts: 6,283
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Re: Cultivation General Discussion [Re: shroomator]
#26095656 - 07/08/19 05:31 AM (4 years, 6 months ago) |
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Depends on how far you want to expand versus how fast you want it to colonize.
You could transfer one quart to ten, or you could transfer one quart to fourty, believe it or not.
-------------------- But if you're in a hurry, and really got to go If you're in a hurry, might have to find out slow That it's one thing to try and another to fly You get there quicker just a step at a time It's one thing to bark, another to bite The show ain't over till you pack up at night
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shroomator
Stranger

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Re: Cultivation General Discussion [Re: filthyknees]
#26095777 - 07/08/19 07:22 AM (4 years, 6 months ago) |
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Thanks, that makes sense . How long can i store a jar after its fully colonized?
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hexkrak
VisualDecintigrator


Registered: 12/26/02
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Re: Cultivation General Discussion [Re: shroomator]
#26095938 - 07/08/19 10:15 AM (4 years, 6 months ago) |
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Quote:
shroomator said: Thanks, that makes sense . How long can i store a jar after its fully colonized?
That depends on a lot of factors. I have had a lot of BRF cakes start fruiting before I'm ready, either because of a hidden contaminate, or maybe fruiting conditions were perfect. On the other hand I've had colonized oats sit around for a couple weeks and they didn't have any signs of pins yet.
--------------------
Always forgive your enemies; nothing annoys them more. --Oscar Wilde
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Brain Bulb
Insane in the Membrane



Registered: 11/09/17
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Quote:
verum subsequentis said: Big fruits in later flushes have less to do with genetics than they do the amount of fruit growing at the same time. If you've ever grown cannabis..... Tomatoes.... Apples..... Any other growing thing I can think of. You'll understand the principle at work.
The best fruit from a heavy first flush pinset is your best bet. Best indicator of fast and desirable genetics is a bad ass fruit among the masses.
Cool thanks! Hopefully Iβll start getting better canopies. :-)
The first flush on my PE var. clone came in slow and were small. 2nd flush actually came in faster and had larger fruit but the clusters were bigger. Could it be bc itβs a 1st gen clone?
Edited by Brain Bulb (07/08/19 11:04 AM)
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verum subsequentis
seeker of truth



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Re: Cultivation General Discussion [Re: Brain Bulb]
#26096018 - 07/08/19 11:22 AM (4 years, 6 months ago) |
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What do you mean "1st Gen clone"? I doubt that's the problem. The spores you started with were most probably from a clone that was most probably multiple generations deep.... Anyway.
PE is a strange variation and behaves rather differently than non mutant cubensis varieties. The first flush can be finicky and hard to get right. Some say you need to let it consolidate longer and some say it's all about a proper casing. I say, and I'm far from totally understanding PE, spawn just like any other cube and apply a nice casing at spawning. I think it's important not to go too high on the spawn ratio with PE too. 1:2 or 1:3 has seemed to work best for me. 1:1 send to cause more of a blobish first flush. This could be tied to longer colonization theory to though.
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Brain Bulb
Insane in the Membrane



Registered: 11/09/17
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Last seen: 6 months, 24 days
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Quote:
verum subsequentis said: What do you mean "1st Gen clone"? I doubt that's the problem. The spores you started with were most probably from a clone that was most probably multiple generations deep.... Anyway.
PE is a strange variation and behaves rather differently than non mutant cubensis varieties. The first flush can be finicky and hard to get right. Some say you need to let it consolidate longer and some say it's all about a proper casing. I say, and I'm far from totally understanding PE, spawn just like any other cube and apply a nice casing at spawning. I think it's important not to go too high on the spawn ratio with PE too. 1:2 or 1:3 has seemed to work best for me. 1:1 send to cause more of a blobish first flush. This could be tied to longer colonization theory to though.
Thatβs why I was asking.
I actually started with a used swab that was supposed to be APE, which after many attempts I was able to get it to fruit. (Due to what I started with I was fighting contaminants). When it did finally fruit they looked nothing like a PE strain. (Will post pics when I can access). The fruit I cloned was from about the 10th flush and the only fruit resembling PE. Since taken from such a late flush its basically starting over with the genetics isnβt it? Again, asking so I know for future clones.
Thanks,
BB
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MLPismyOPSEC
That One Ponyfucker


Registered: 11/13/18
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Loc: Equestria? Mordor? Wester...
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Could use some help diagnosing my tubs. Inoculated grain on 5/1, Spawned on 6/1, so i know the genetics have been horrendously slow from the start. First 2 weeks, things went well and the pinset looked great, but the big factor against me is the furnace did not run at all so there was no air movement. Week 3 it got hot outside and the A/C ran a lot, so they definitely got some fresh air. Now, they have seemed to stall out. They've been at 69-71* F at all times, the only change really is air current.
This was 7/1:
  
This is today:
  

It looks dry as hell on the surface, but underneath still has so much moisture on the tubs, so that leads me to believe that's not the problem. But on the left and right tubs, mycelium has started to climb some stipes and caps and take over. Are these even viable to keep going or should i chuck them? My gut instinct is that i don't trust how long they've been going so i should probably throw them out.
Edited by MLPismyOPSEC (07/08/19 12:15 PM)
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verum subsequentis
seeker of truth



Registered: 03/22/16
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Re: Cultivation General Discussion [Re: Brain Bulb]
#26096114 - 07/08/19 12:43 PM (4 years, 6 months ago) |
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Quote:
The fruit I cloned was from about the 10th flush and the only fruit resembling PE. Since taken from such a late flush its basically starting over with the genetics isnβt it? Again, asking so I know for future clones.
Thanks,
BB
Absolutely not. It doesn't matter what flush a fruit is from. Clones never reset genetics. That's the whole point of cloning. To preserve genetics. Spores alone "reset genetics" and they don't even really do that. They take you back a bit in the genetic history (lineage) but don't reset it. Your spores have a history as old as time.
Edited by verum subsequentis (07/08/19 12:44 PM)
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Brain Bulb
Insane in the Membrane



Registered: 11/09/17
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Quote:
verum subsequentis said:
Quote:
The fruit I cloned was from about the 10th flush and the only fruit resembling PE. Since taken from such a late flush its basically starting over with the genetics isnβt it? Again, asking so I know for future clones.
Thanks,
BB
Absolutely not. It doesn't matter what flush a fruit is from. Clones never reset genetics. That's the whole point of cloning. To preserve genetics. Spores alone "reset genetics" and they don't even really do that. They take you back a bit in the genetic history (lineage) but don't reset it. Your spores have a history as old as time.
Right on, thanks!
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Brain Bulb
Insane in the Membrane



Registered: 11/09/17
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Re: Cultivation General Discussion [Re: MLPismyOPSEC]
#26096148 - 07/08/19 01:00 PM (4 years, 6 months ago) |
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Quote:
MLPismyOPSEC said: Could use some help diagnosing my tubs. Inoculated grain on 5/1, Spawned on 6/1, so i know the genetics have been horrendously slow from the start. First 2 weeks, things went well and the pinset looked great, but the big factor against me is the furnace did not run at all so there was no air movement. Week 3 it got hot outside and the A/C ran a lot, so they definitely got some fresh air. Now, they have seemed to stall out. They've been at 69-71* F at all times, the only change really is air current.
This was 7/1:
  
This is today:
  

It looks dry as hell on the surface, but underneath still has so much moisture on the tubs, so that leads me to believe that's not the problem. But on the left and right tubs, mycelium has started to climb some stipes and caps and take over. Are these even viable to keep going or should i chuck them? My gut instinct is that i don't trust how long they've been going so i should probably throw them out.
What size tub and what spawn:sub ratio did you use? When the temp dropped it could have caused them to stall.
Also MS or spores -> agar?
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MLPismyOPSEC
That One Ponyfucker


Registered: 11/13/18
Posts: 884
Loc: Equestria? Mordor? Wester...
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Re: Cultivation General Discussion [Re: Brain Bulb]
#26096227 - 07/08/19 02:06 PM (4 years, 6 months ago) |
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Quote:
Brain Bulb said: What size tub and what spawn:sub ratio did you use? When the temp dropped it could have caused them to stall.
Also MS or spores -> agar?
66qt tubs, 1:1.5 ratio, sub depth is about 3" on all. The right tub was closer to 1:1.7 and 3.5" but that is splitting hairs. The temp didn't drop though, just that they now have cool air blowing near them. While the A/C was running, the substrate still checked out at ~70*. MS to agar, and i know the plates weren't perfectly clean. The plates looked visually good but my spawn was a little bacterial. To me, the pinset looks like it DGAF about the bacteria
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hypercubeididnt
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Re: Cultivation General Discussion [Re: MLPismyOPSEC]
#26096347 - 07/08/19 03:23 PM (4 years, 6 months ago) |
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hello i dont know how much information i should give
i have grown some spores in a special grow kit(this special grow kit does not follow a/the pk tek) for 5 weeks
i am now ready for the next stage but i dont fully understand what i should be doing next with the perlite and its exact position(s), i need to take off the lid of the special grow kit now that my spores have turned to strains and filled the special grow box and then place the grown spores>strain into a humidity tent(bag) with damn perlite.
my question involves the perlite(damp) do i put it onto the top of the grown strains? or around the side of the plastic container? or both? whilst inside a humidity tent...?
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Failboat
Fuck Up

Registered: 02/01/18
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Sounds like you dont need perlite at all. Pics, if possible, would help mucho
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Crackatoa
Stranger in a strange land



Registered: 03/31/19
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Perlite is always on the bottom with a piece of foil between it and the mycellium. Or it's on a rack above the perlite. If you go foil, you want it just big enough that the mycellium isn't touching the perlite. Pics would be helpful if pics wasn't down at the moment.
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Sherlock Shrooms
Neophyte



Registered: 06/25/19
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Re: Cultivation General Discussion [Re: Crackatoa]
#26097380 - 07/09/19 02:17 AM (4 years, 6 months ago) |
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Is this ready to spawn or should it sit and consolidate to become a denser white?
-------------------- "When you pass through the waters, I will be with you; And through the rivers, they shall not overflow you. When you walk through the fire, you shall not be burned, Nor shall the flame scorch you."

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Caps McGee
Grandaddy Smurfshack



Registered: 10/28/17
Posts: 14,357
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If it's clean, doesn't really matter... wouldn't hurt to let it consolidate... bacterial issues are caused/exacerbated by excess moisture... I wouldn't call that fully colonized... this is:
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Sherlock Shrooms
Neophyte



Registered: 06/25/19
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Re: Cultivation General Discussion [Re: Caps McGee]
#26097397 - 07/09/19 02:32 AM (4 years, 6 months ago) |
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Boom, will wait thanks. Spawning once all the grains are covered even if the myc is a bit thin like in my pic has been my standard. I wonder if some of my struggles can be attributed to that
-------------------- "When you pass through the waters, I will be with you; And through the rivers, they shall not overflow you. When you walk through the fire, you shall not be burned, Nor shall the flame scorch you."

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Caps McGee
Grandaddy Smurfshack



Registered: 10/28/17
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Loc: ally known as ...
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I mean... I kind of doubt it the determining factor alone... perhaps not helpful, but I believe that if the culture is healthy, and the sub isn't overhydrated, it should colonize before contaminant spores have a chance to germinate and grow to be an issue... it may be a cumulative effect of many small things, but I believe there to be enough culture there to do it's thing... we shake our grain before spawning so much of it is bare anyways... Idk mane
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