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ibuprofemin
Stranger

Registered: 04/03/19
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Re: Cultivation General Discussion [Re: Manic503]
#25952654 - 04/24/19 11:33 AM (4 years, 9 months ago) |
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If I put some colonized agar plates In the fridge for a while, would they be ok to go to grain afterwards? Have some that I wanna use, but waiting for some other plates to catch up so I can make all the jars at once. Thanks!
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Yesum
Furry as Fuc



Registered: 11/05/12
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Loc: Central Part of Town
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Re: Cultivation General Discussion [Re: ibuprofemin]
#25952694 - 04/24/19 11:58 AM (4 years, 9 months ago) |
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Technically you can. So long as you have them stored and sealed up in there u should be fine. I've used plates not straight out of the fridge but I let them acclimate a few days first. I just dont like doing it. Feels weird. I'd rather make at least 1 transfer first.
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ibuprofemin
Stranger

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Re: Cultivation General Discussion [Re: Yesum]
#25952714 - 04/24/19 12:09 PM (4 years, 9 months ago) |
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Quote:
Yesum said: Technically you can. So long as you have them stored and sealed up in there u should be fine. I've used plates not straight out of the fridge but I let them acclimate a few days first. I just dont like doing it. Feels weird. I'd rather make at least 1 transfer first.
They’ve been transferred already, I just have a couple other things going that need to catch up and probably need a few days. I could alternatively keep them out but wouldn’t want them to overflow the plate or get contaminated (looking great now hah).
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icetech



Registered: 08/21/17
Posts: 3,450
Loc: FSM's loving noodles.
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Re: Cultivation General Discussion [Re: ibuprofemin]
#25952718 - 04/24/19 12:13 PM (4 years, 9 months ago) |
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Quote:
ibuprofemin said: If I put some colonized agar plates In the fridge for a while, would they be ok to go to grain afterwards? Have some that I wanna use, but waiting for some other plates to catch up so I can make all the jars at once. Thanks!
I have used plates i kept in the fridge for over a year, just make sure the top is tight when you put them in.. pulled a few out that had dried out I usually let them sit out at room temp for a day or so to wake back up, but not needed..
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ShaperDreaming
Weirdo



Registered: 10/30/18
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Re: Cultivation General Discussion [Re: icetech]
#25952726 - 04/24/19 12:17 PM (4 years, 9 months ago) |
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For those of you who use a propane torch to flame sterilize: how long do those little tanks last?
I ask because I'm reviewing all my sterile techniques and it seems like this is something I should probably step up to, and I have never used these before so I don't want to suddenly run out.
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LtLurker
Lost Sailor



Registered: 01/03/18
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Re: Cultivation General Discussion [Re: ibuprofemin]
#25952727 - 04/24/19 12:17 PM (4 years, 9 months ago) |
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maybe it as a form of appeal to authority in some ways, but I'm thinking of it more like experience. For example, if I ran a construction company and one guy with 30 years thinks we should do a project one way, while a guy in his 2nd year suggests a different method, which would you be more inclined to listen to? I might hear out the newer guy, but where the 2 conflict I'd lean towards the opinion of the experienced guy. In this situation though, it's not just 1 v 1, it's the consensus of many people over years doing it successfully, repeatedly.
I'm not saying this to beat you down or call you wrong, just trying to explain context/perspective. In the end, spraying things like lysol isn't exactly going to hurt, so I think of it as benign and not really stress about it beyond comfort. I was psyched when I learned I was spraying lysol for no real reason on my first couple grows and didn't have to keep working in the smelly stuff. Valar Dohaeris
@ibuprofen Yes plates store fine in the fridge just like slants. If it's been sitting for long, I might transfer just to ensure it's still clean.
Edited by LtLurker (04/24/19 12:19 PM)
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van hatton
Still a noob


Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Re: Cultivation General Discussion [Re: Yesum]
#25952748 - 04/24/19 12:26 PM (4 years, 9 months ago) |
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Quote:
Yesum said: Technically you can. So long as you have them stored and sealed up in there u should be fine. I've used plates not straight out of the fridge but I let them acclimate a few days first. I just dont like doing it. Feels weird. I'd rather make at least 1 transfer first.
I've been pulling straight out the fridge since November.
Only downside I see is hidden contam (lost 2~ plates to Tam in fridge)
And your cultures dying eventually or you running out and not realizing it (my problem)
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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El Chupacabra
Stoned Ape



Registered: 01/07/19
Posts: 676
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Quote:
ShaperDreaming said: For those of you who use a propane torch to flame sterilize: how long do those little tanks last?
I ask because I'm reviewing all my sterile techniques and it seems like this is something I should probably step up to, and I have never used these before so I don't want to suddenly run out.
I'm on the same bottle I used all thru winter to get the wood stove going and grill and now I've done maybe 5 sleeves of dishes (where I needed the torch). I imagine with whats left + the other bottle it will probably last me into 2020.
Man I just nabbed 100lbs of oats for $17. Ready to try something other than sticky milo. Still have a bit of that before I run out. I used to like milo and now I'm like FUCK that milo shit!
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Tweeq
Tweeq of Nature


Registered: 06/07/18
Posts: 2,043
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Quote:
ShaperDreaming said: For those of you who use a propane torch to flame sterilize: how long do those little tanks last?
I ask because I'm reviewing all my sterile techniques and it seems like this is something I should probably step up to, and I have never used these before so I don't want to suddenly run out.
I got this little tank and it's still my first. It says it'll burn for 2,5 hrs fully opened but you never need that.

Spare can text
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ShaperDreaming
Weirdo



Registered: 10/30/18
Posts: 3,429
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Quote:
El Chupacabra said: I'm on the same bottle I used all thru winter to get the wood stove going and grill and now I've done maybe 5 sleeves of dishes (where I needed the torch). I imagine with whats left + the other bottle it will probably last me into 2020.
Cool. Is it pretty easy to tell by weight when it's running low?
Quote:
El Chupacabra said: Man I just nabbed 100lbs of oats for $17. Ready to try something other than sticky milo. Still have a bit of that before I run out. I used to like milo and now I'm like FUCK that milo shit!
Nice haul. I have 25# of milo that I bought becasue I thought I was buying millet but I was just excited in the moment. I thought it would be better for surface area for my master grain jars... but it's kinda about the same size as oats with more difficult prep. Anyways, I'm not excited to use that bag
Edited by ShaperDreaming (04/24/19 01:00 PM)
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El Chupacabra
Stoned Ape



Registered: 01/07/19
Posts: 676
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Quote:
ShaperDreaming said:
Quote:
El Chupacabra said: I'm on the same bottle I used all thru winter to get the wood stove going and grill and now I've done maybe 5 sleeves of dishes (where I needed the torch). I imagine with whats left + the other bottle it will probably last me into 2020.
Cool. Is it pretty easy to tell by weight when it's running low?
Quote:
El Chupacabra said: Man I just nabbed 100lbs of oats for $17. Ready to try something other than sticky milo. Still have a bit of that before I run out. I used to like milo and now I'm like FUCK that milo shit!
Nice haul. I have 25# of milo that I bought becasue I thought I was buying millet but I was just excited in the moment. I thought it would be better for surface area for my master grain jars... but it's kinda about the same size as oats with more difficult prep. Anyways, I'm not excited to use that bag 
Oh yea. You can feel it in there and hear it slosh. Even after it seemingly runs dry you could probably manage 1 more night of myco work before having to change it.
I think milo is the most photogenic of the grains but it's a PITA to get it prepped perfectly without too many busted grains and stickyness. Plus I think oats might smell less bad too.
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Caps McGee
Grandaddy Smurfshack



Registered: 10/28/17
Posts: 14,357
Loc: ally known as ...
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Re: Cultivation General Discussion [Re: Manic503]
#25952836 - 04/24/19 01:13 PM (4 years, 9 months ago) |
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Quote:
Manic503 said:
Quote:
tryptkaloids said:
Quote:
Manic503 said: but doing that correlates with 0 contams so I do it just to feel better.
Some people pray, I use bleach. Lol
Correlation is not causation. Physics is our best tool, not chemistry
No argument on both(either) points but since I am not running some double blind experiment to determine whether the well established fact that a 10% bleach solution kills mold spores or not, I don’t see your point (anecdotally, its a correlation, academically its kindergarten).
People don’t want to “waste” the time or energy cleaning for whatever reason? Cool. You do you. But stating that cleaning your work area with a disinfectant won’t reduce (not to be confused with “eliminate”) the risk of contams is absurd. This has been drilled to death in other threads. Peace my brethren. Cheers!
The argument isn't that it doesn't reduce risk, it's that ; with proper sterile technique and thoughtful hand placement, all the harmful chemicals/fumes are unecessary... cleaning with bleach, or even spraying the sides with soapy water, is unecessary, but not a waste; if it makes you feel better, or need to buffer questionable technique... I ran a dry SAB without gloves or iso for month: been a few sessions I forgot to wash my hands ... bc I'm anal (teehee) about where I grab jar/dish lids (avoiding the lip/rim), and not having more of my instruments or self over receiving media than needed to do work, while using slow concise movements, it's not presented issue... no one is saying you shouldn't; as you said, you do you, just eluding that you don't have to, and could save time, effort, worry, money, supplies etc... maybe give it a shot? Lol, once you go dry SAB, you'll want all that time spraying and wiping back lol
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van hatton
Still a noob


Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Re: Cultivation General Discussion [Re: Caps McGee]
#25952849 - 04/24/19 01:19 PM (4 years, 9 months ago) |
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-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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muhfreedumb


Registered: 11/30/17
Posts: 199
Loc: Canadia
Last seen: 3 years, 9 months
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Re: Cultivation General Discussion [Re: Caps McGee]
#25952862 - 04/24/19 01:26 PM (4 years, 9 months ago) |
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For me whenever I don't spray enough soap I get mold filled dishes. That could be due to my SAB being too big (I think 104ishL) or my room too drafty. Maybe in my case the soapy spray works to actively settle/pull the dirty air and keeps it that way long enough for me to clumsily complete my SAB work. If my technique was better maybe I'd be fine not relying on the soap.
P.S to shaper dreaming from early posts: I wait like 30 seconds post spraying (long enough to wipe my hands with iso), after 10 min of work half the soap has dried out, maybe that's something to try changing?
Edited by muhfreedumb (04/24/19 01:27 PM)
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ShaperDreaming
Weirdo



Registered: 10/30/18
Posts: 3,429
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Re: Cultivation General Discussion [Re: muhfreedumb]
#25952875 - 04/24/19 01:32 PM (4 years, 9 months ago) |
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Quote:
muhfreedumb said:
P.S to shaper dreaming from early posts: I wait like 30 seconds post spraying (long enough to wipe my hands with iso), after 10 min of work half the soap has dried out, maybe that's something to try changing?
Thanks, I'm going to change up over to a dry box and I'm going to up to a propane torch for my flame sterlizing. I've had a double alcohol burner, which is better than a single, but still isn't super balanced when heating.
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Caps McGee
Grandaddy Smurfshack



Registered: 10/28/17
Posts: 14,357
Loc: ally known as ...
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Maybe slow down? Keep the air still lol... whatever works...
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Failboat
Fuck Up

Registered: 02/01/18
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Have you been sure your AC isn't causing a draft? I know I overlook this sometimes.
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van hatton
Still a noob


Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Re: Cultivation General Discussion [Re: Failboat] 1
#25952913 - 04/24/19 01:51 PM (4 years, 9 months ago) |
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I used to use a 50 gal tote. Ac and or heater going same time just not at it.
Have had my fair share of mold problems
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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ShaperDreaming
Weirdo



Registered: 10/30/18
Posts: 3,429
Loc: United States
Last seen: 2 years, 3 days
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Re: Cultivation General Discussion [Re: Failboat] 1
#25952925 - 04/24/19 01:58 PM (4 years, 9 months ago) |
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Haha, nah, it's neither of those things, but thanks. I don't even have my A/C in yet for the season. My mycology work lately has just been a huge crap shoot and I'm having issues I didn't have at my last place ever, so it's just a constant elimination of possible technique issues. Watched all of Bod's SAB videos earlier today, I don't feel like I'm that far off from his techniques, but I picked up a couple things I'll be using.
I also bought some wire grid cooling racks to use so I keep things off the "ground" better. I'll be picking up a propane torch too. I've started a more rigorous cleaning schedule for my whole house as well, and I'm running an air purifier in the space I'm running mycology work (not while doing the work, to preemptively get the contam).
I know this work can be done in "dirty" spaces, so I'm just trying to work on my technique and changing/improving what I'm doing now. I had a less than 10% contam rate in my last place, it jumped up to about 40% w/o me doing anything different but moving houses I tried really hard to figure out a way to make a LFH work in my space but I just don't have enough room (I live in a studio apartment around 550sf, and unless that fucker is doubling as a TV, I'm stuck with a SAB until at least next year).
My next step may be to do a built-in SAB in my closet, I recently realized that I have enough space in there and that would just be a few pieces of wood and a plexiglass face. I have these weird storage spaces in my place (100+ year old building), and this closet is only like 12" deep, but it's behind doors so I could make the front of the "SAB" come out to the edge of the closet door. I has ideas...
Edited by ShaperDreaming (04/24/19 01:59 PM)
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Failboat
Fuck Up

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