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OfflineGeinstein Happy Birthday!
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Registered: 01/25/18
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Re: Cultivation General Discussion [Re: tryptkaloids]
    #25950224 - 04/23/19 02:40 AM (4 years, 9 months ago)

Printing shrooms with the same strictness as choosing shrooms to clone
Input?


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Nothing breads nothing


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OfflineSecotoid
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Registered: 04/21/19
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Re: Cultivation General Discussion [Re: Secotoid]
    #25950226 - 04/23/19 02:41 AM (4 years, 9 months ago)

Your still air box is only still if nothing moves. Your still air box is only clean if nothing else plasmic exists in it. Both of these assumptions are self evidently ridiculous. You continue to conflate the unlikeliness of contamination with impossibility and to forget that I'm not arguing anything but that the risk exists and is easily mitigated at cost substantially smaller than the cost of failure. That's the whole argument. I do not know how much more clearly it can be made.

Quote:

It's not an assumption. I can be certain of myself because all the testing has been done for me. People have been moving agar through sabs for decades proving that the problems come from mixing non sterile tools with sterile media





When you make statements like this I become uncertain that you know what assumption or certain mean. You are still arguing for certainty from probability. Nobody here is or has at any point been arguing that your process is likely to fail.


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InvisibleYesum
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Re: Cultivation General Discussion [Re: Geinstein]
    #25950230 - 04/23/19 02:48 AM (4 years, 9 months ago)

Quote:

Geinstein said:
Printing shrooms with the same strictness as choosing shrooms to clone
Input?




What do u mean by strictness?  If u mean print fruits with desired traits. I dont think it matters as much because your spores  have TONS of genetics in there.

Print them all. Big and tall, and short and stumpy. If they drop spores there good to go.

Might be a different story if your doing something like a stabilization project.


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OfflineNosejob12
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Registered: 02/13/19
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Re: Cultivation General Discussion [Re: Yesum]
    #25950238 - 04/23/19 03:04 AM (4 years, 9 months ago)

Is it possible to share a small out door bed between opposite season species to a worthwhile
Potential? Specifically azzies and ovois? Tia


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InvisibleYesum
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Re: Cultivation General Discussion [Re: Nosejob12]
    #25950246 - 04/23/19 03:10 AM (4 years, 9 months ago)

I'm not a outdoor grower of mush. But if you were to share the same exact bed. You'd have to tear up an already established bed to put in your new one. My guess is you'd need 2 different beds.


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OfflineCamera93
We got dicks like Jesus
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Registered: 08/15/18
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Re: Cultivation General Discussion [Re: Yesum]
    #25950305 - 04/23/19 05:21 AM (4 years, 9 months ago)

Do you prefer swabs or a loop for taking spores to agar?


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All I need are some tasty waves, a cool buzz, and I’m fine.

Whatever you decide won’t really impact our survival
Close your eyes, and do the best that you can



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InvisibleYesum
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Re: Cultivation General Discussion [Re: Camera93]
    #25950336 - 04/23/19 06:21 AM (4 years, 9 months ago)

I use my blade.


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Offlinemushroomnate
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Re: Cultivation General Discussion [Re: Yesum]
    #25950347 - 04/23/19 06:34 AM (4 years, 9 months ago)

Quote:

Yesum said:
I use my blade.



:whathesaid:
I like to scrape the spores onto on the tip of the blade,and "cut" them into the agar.


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BRF to bulk/Cakes as spawn


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OfflineCamera93
We got dicks like Jesus
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Re: Cultivation General Discussion [Re: mushroomnate]
    #25950350 - 04/23/19 06:37 AM (4 years, 9 months ago)

Quote:

mushroomnate said:
Quote:

Yesum said:
I use my blade.



:whathesaid:
I like to scrape the spores onto on the tip of the blade,and "cut" them into the agar.




I'll for sure have to try this. I'm still inexperienced and have only gone spore to agar with a loop. But in another thread I seen someone bury the swab into the dish and leave it.


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All I need are some tasty waves, a cool buzz, and I’m fine.

Whatever you decide won’t really impact our survival
Close your eyes, and do the best that you can



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InvisibleBph
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Re: Cultivation General Discussion [Re: Camera93]
    #25950426 - 04/23/19 07:53 AM (4 years, 9 months ago)

Lol.  Now I'm worried about dropping whole plates to grain. Is it just me or do all growers go through phases?  At first I was cluless and felt as though I got lucky. Then I thought I had it all figured out and was blessed. Now im back to cluless and cursed lol. Reboot is in progress ATM so hopefullly a new phase is around the corner cause this one sucks.


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Offlineicetech
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Re: Cultivation General Discussion [Re: Bph]
    #25950440 - 04/23/19 08:01 AM (4 years, 9 months ago)

Quote:

Bph said:
Lol.  Now I'm worried about dropping whole plates to grain. Is it just me or do all growers go through phases?  At first I was cluless and felt as though I got lucky. Then I thought I had it all figured out and was blessed. Now im back to cluless and cursed lol. Reboot is in progress ATM so hopefullly a new phase is around the corner cause this one sucks.




Every grow i feel like a fuckwit at some point (cause i am) my current one is just 4 shoeboxes sitting in a corner stacked up... all i did was glance at the sides and they never looked like they were colonizing.. last night i look and the fuckers are full of mushrooms.. hows that for stupid?


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Invisibleforagedfungus
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Re: Cultivation General Discussion [Re: icetech] * 2
    #25950448 - 04/23/19 08:06 AM (4 years, 9 months ago)

This graph is referencing ID/taxonomy, but it kind of applies here too.



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Offlineicetech
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Re: Cultivation General Discussion [Re: foragedfungus]
    #25950485 - 04/23/19 08:31 AM (4 years, 9 months ago)

Quote:

foragedfungus said:
This graph is referencing ID/taxonomy, but it kind of applies here too.






that pretty much covers it :smile:


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OfflineCamera93
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Re: Cultivation General Discussion [Re: icetech]
    #25950495 - 04/23/19 08:41 AM (4 years, 9 months ago)

Does anyone use this pf tek for going from MS to find a clone?

or do most of you like to just bulk out a MS grow, select from there and move on?


--------------------
All I need are some tasty waves, a cool buzz, and I’m fine.

Whatever you decide won’t really impact our survival
Close your eyes, and do the best that you can



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OfflineShaperDreaming
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Re: Cultivation General Discussion [Re: mushroomnate]
    #25950529 - 04/23/19 09:08 AM (4 years, 9 months ago)

Quote:

mushroomnate said:
Quote:

Yesum said:
I use my blade.



:whathesaid:
I like to scrape the spores onto on the tip of the blade,and "cut" them into the agar.



:whathesaid:

I like that ID chart. I'm feeling at the bottom of that valley.


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OfflineFailboat
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Registered: 02/01/18
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Re: Cultivation General Discussion [Re: Camera93]
    #25950542 - 04/23/19 09:26 AM (4 years, 9 months ago)

Quote:

Camera93 said:
Quote:

mushroomnate said:
Quote:

Yesum said:
I use my blade.



:whathesaid:
I like to scrape the spores onto on the tip of the blade,and "cut" them into the agar.




I'll for sure have to try this. I'm still inexperienced and have only gone spore to agar with a loop. But in another thread I seen someone bury the swab into the dish and leave it.



I use a sterilized qtip to make streaks.


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Invisiblevan hattonFacebookDiscord
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Re: Cultivation General Discussion [Re: Failboat]
    #25950712 - 04/23/19 11:28 AM (4 years, 9 months ago)

I know I'm a bit late to this conversation and didn't read alot buttttt

I took a josex poke and put it directly to grain.
It took about 28 days to hit 100% totally possible. But that's alot of work.

I just take my petri cut it into 3rda and transfer to 3 jars. Edges and all. My contam rate is like 2-3% which is hella good.

I double wrap my plates :shrug: I never have a problem with edge contam.

Flowhood ftw.

And I don't see a reason to mist sabs hell yeah I'm still on that train. My contams went from 40% to 99% clean plates after the switch.

While I can't say they r related and I just became a master of agar overnight (possible) it can't be ruled out.
:bongload:


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If I ever give out misinformation please inform me so I can have the correct information. :cheers:

Tmethyl said:
Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy.

Caps McGee said:
:thumbsup:
Fun part is figuring out what works best for you


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Offlineibuprofemin
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Re: Cultivation General Discussion [Re: van hatton]
    #25950840 - 04/23/19 12:50 PM (4 years, 9 months ago)

No growth in MS syringe to grain after 2 weeks, should I just toss them? Not contaminated, but have some agar clones that are turning out successful and I’d rather use the jars for something that would work. Have 5 clones made up that should be ready in a couple days (did first transfer yesterday and the plates looked nice and clean) and then some plates from a spore swab I took of nice fruits that are also doing well.


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Offlinetryptkaloids
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Re: Cultivation General Discussion [Re: ibuprofemin]
    #25950867 - 04/23/19 01:07 PM (4 years, 9 months ago)

You can reinoculate them with agar


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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Offlineibuprofemin
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Re: Cultivation General Discussion [Re: tryptkaloids]
    #25950873 - 04/23/19 01:15 PM (4 years, 9 months ago)

Quote:

tryptkaloids said:
You can reinoculate them with agar




Seems relatively risky, no? The oats are cheap, wouldn’t it be better to just pitch (can bury the oats outside) and make anew? Excited about these clones, they had uniform growth radially from the piece of tissue I biopsied, so just waiting for them to grow out on new plates and I think they’ll do well, wouldn’t wanna take a step backwards if those jars are screwed up!


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