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InvisiblemushboyMDiscord
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Re: Cultivation General Discussion [Re: muhfreedumb]
    #25949909 - 04/22/19 09:49 PM (4 years, 9 months ago)

all i have is best methods:lol:

i rely on the other smarter people here to do all that analytical mumbo jumbo:typing:


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Offlinetryptkaloids
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Re: Cultivation General Discussion [Re: tryptkaloids]
    #25949910 - 04/22/19 09:50 PM (4 years, 9 months ago)

Tell me how to do said analysis and id love to dive down that rabbit hole. The issue is we dont have the resources cannabis growers do.

Im not trying to convince him my way is better, just understand why he thinks his is


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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Offlinemuhfreedumb
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Re: Cultivation General Discussion [Re: mushboy]
    #25949911 - 04/22/19 09:52 PM (4 years, 9 months ago)

Exactly! Best methods for you, that's about the best any of us can hope for.

The analytical stuff doesn't really even matter for everyone doing this for fun.


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InvisiblePsicomb
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Re: Cultivation General Discussion [Re: muhfreedumb]
    #25949915 - 04/22/19 09:54 PM (4 years, 9 months ago)

:popcorn: awesome discussion folks


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When we constantly pull things apart trying to see how it works, we may end up with only an understanding of how to destroy something
- nick sand


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Offlinemuhfreedumb
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Re: Cultivation General Discussion [Re: tryptkaloids] * 1
    #25949916 - 04/22/19 09:54 PM (4 years, 9 months ago)

The kicker is that you could both do the same analysis, do 50% his way and 50% whole plate, and get completely different results!

It's all so situational since we're all doing this in different set-ups using what we all prefer and have available.


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InvisibleAyePlusS
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Re: Cultivation General Discussion [Re: muhfreedumb]
    #25949943 - 04/22/19 10:07 PM (4 years, 9 months ago)

I usually make 2-4 masters off a petri, I cut a cross off to the side of the donor wedge and then cut a digonal so the donor piece doesn’t make it onto grain just in case, when I make 4 it takes 2 shakes and about three weeks for full colonization. Not exactly sure why I do it that way but it works so :shrug:


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OfflineSecotoid
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Re: Cultivation General Discussion [Re: tryptkaloids] * 1
    #25949957 - 04/22/19 10:19 PM (4 years, 9 months ago)

I elaborated extensively and clearly. The argument with it's premises and painstaking defense of every point you have questioned in my reasoning are all there to read if you just turn off your stubborn. I even offered a fair supposition of the actual contention between your view to the matter and mine. Mine takes a precaution against a particular and real set of risks which you choose to either embrace, ignore or deny exist because you feel they shouldn't. Your results are more than good enough to justify your approach, your argument simply is poorly conceived. As you chose to instigate argument against my approach I am none the less guaranteed to defend it. It is sound.


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Offlinetryptkaloids
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Re: Cultivation General Discussion [Re: Secotoid]
    #25950125 - 04/23/19 12:55 AM (4 years, 9 months ago)

The issue is you're using unneccesary big words that my vocab cant handle. Like, for real, why do you need to yse words like supposition and contention?

Im not saying it won't work, just that its a paranoid way of going about things. What benefit is actually gained from using such a small inoculation point?

I just dont feel like it's a good use of your time to spend weeks cleaning a culture to wait forever to use your master, just because your culture might be dirty...  If you think there's something on your plate that shouldn't be there you need to keep transferring, not take that plate to grain.

I just feel Like if you were trying to mitigate risk you would strictly use the cleanest cultures instead of trying to use a tiny tissue sample in an effort to avoid contam.


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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OfflineSecotoid
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Re: Cultivation General Discussion [Re: tryptkaloids]
    #25950140 - 04/23/19 01:20 AM (4 years, 9 months ago)

You don't have to wait forever. it's an extra couple days. We both know  your approach works when done right and you've shown that here. You aren't being asked to adopt mine. Our entire debate was about no more than the merits of my approach which are real and have been elaborated thoroughly. You know they it isn't required to have success and I know that too. :thumbup:


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InvisibleYesum
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Re: Cultivation General Discussion [Re: Secotoid]
    #25950192 - 04/23/19 02:13 AM (4 years, 9 months ago)

Well I wonder how it is I can drop 3/4 of a 100mm plate into my blender jar, with sterile water. If clean agar culture was a vector. Making agar LI would never work. I dont understand this particular statement


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InvisibleYesum
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Re: Cultivation General Discussion [Re: Secotoid]
    #25950194 - 04/23/19 02:15 AM (4 years, 9 months ago)

Quote:

Secotoid said:
. Every square mm over what is required is additional risk. Even if it is perfectly clean, just moving extra surface area of agar around in a transfer is additional risk, however small.




Particularly this.


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OfflineSecotoid
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Re: Cultivation General Discussion [Re: Yesum]
    #25950201 - 04/23/19 02:20 AM (4 years, 9 months ago)

Quote:

Yesum said:
If clean agar culture was a vector.




When and where was it ever suggested that clean agar was a vector for contamination? It's like a couple of you are trying really hard to miss the point.


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OfflineSecotoid
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Re: Cultivation General Discussion [Re: Yesum]
    #25950205 - 04/23/19 02:26 AM (4 years, 9 months ago)

Quote:

Yesum said:
Particularly this.




I see what you mean. The assertion here has nothing to do with the condition of the agar at all. If you move a large thing through a potential field of contaminates, it is more likely to intersect them than a small thing moving the same distance through the same vector in the same field. Again, we all know and understand that the ideal is perfectly clean still air or perfectly filtered flow of air and we are fools if we pretend that either condition is certain. The vector exists.


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InvisibleYesum
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Re: Cultivation General Discussion [Re: Secotoid]
    #25950206 - 04/23/19 02:27 AM (4 years, 9 months ago)

Right above your post. I quoted it

I'm def not trying to argue. Was just very confused by that statement. thought maybe u knew something I didnt.


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OfflineSecotoid
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Re: Cultivation General Discussion [Re: Yesum]
    #25950207 - 04/23/19 02:28 AM (4 years, 9 months ago)

Yeah, I saw that after replying. My apologies Yesum


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Offlinetryptkaloids
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Re: Cultivation General Discussion [Re: Secotoid]
    #25950210 - 04/23/19 02:30 AM (4 years, 9 months ago)

That's only an issue if you ignore basic sterile technique... The air in a sab isnt a field of contaminates. I can say that with certainty. Thats why we mist them.


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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Offlinetryptkaloids
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Registered: 02/08/15
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Re: Cultivation General Discussion [Re: tryptkaloids]
    #25950211 - 04/23/19 02:31 AM (4 years, 9 months ago)

It's not open air. It might make sense if you're working in open air


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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OfflineSecotoid
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Re: Cultivation General Discussion [Re: tryptkaloids]
    #25950212 - 04/23/19 02:32 AM (4 years, 9 months ago)

Quote:

tryptkaloids said:
That's only an issue if you ignore basic sterile technique... The air in a sab isnt a field of contaminates. I can say that with certainty. Thats why we mist them.




And again, that's only valid if you assume the ideal is always true.


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InvisibleYesum
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Re: Cultivation General Discussion [Re: Secotoid]
    #25950214 - 04/23/19 02:32 AM (4 years, 9 months ago)

No apologies needed.  Appreciate it though :thumbup:


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Offlinetryptkaloids
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Re: Cultivation General Discussion [Re: Yesum]
    #25950219 - 04/23/19 02:36 AM (4 years, 9 months ago)

It's not an assumption. I can be certain of myself because all the testing has been done for me. People have been moving agar through sabs for decades proving that the problems come from mixing non sterile tools with sterile media


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


Extras: Filter Print Post Top
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