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mushboy
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Re: Cultivation General Discussion [Re: tryptkaloids]
#25949711 - 04/22/19 08:04 PM (4 years, 9 months ago) |
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Quote:
tryptkaloids said: Makes things way fast and let's my outrun potential bacteria.
why do you have bacteria in the first place? i like secotoids thinking
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Failboat
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Re: Cultivation General Discussion [Re: mushboy]
#25949715 - 04/22/19 08:07 PM (4 years, 9 months ago) |
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A nickel to quarter sized wedge of SAGAR should go quickly. Colonization taking forever is not what you want.
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Secotoid
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Re: Cultivation General Discussion [Re: tryptkaloids]
#25949730 - 04/22/19 08:11 PM (4 years, 9 months ago) |
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A master jar is a master culture. It is the one upon which all downstream culture in a series depends. Otherwise it's just some jar. I see what you mean though and I think our contention lays more in debate of risk mitigation vs time management. I err on the side of risk mitigation when an order of magnitude greater resources depend on my assumptions. Even taking time as a resource, my method costs far less in any case but the idea. Least harm is also key.
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Secotoid
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Re: Cultivation General Discussion [Re: mushboy]
#25949733 - 04/22/19 08:12 PM (4 years, 9 months ago) |
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My thinking depends in part on the allowance that known or unknown one might have bacteria, I just tried to achieve an approach which economizes risk/benefit in either case.
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mushboy
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Re: Cultivation General Discussion [Re: Secotoid]
#25949735 - 04/22/19 08:14 PM (4 years, 9 months ago) |
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Quote:
Secotoid said: I err on the side of risk mitigation when an order of magnitude greater resources depend on my assumptions.
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tryptkaloids
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Re: Cultivation General Discussion [Re: Secotoid]
#25949739 - 04/22/19 08:15 PM (4 years, 9 months ago) |
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if we're talking odds a small wedge may take so long the grain could dry out and cause stalling. Quote:
mushboy said:
Quote:
tryptkaloids said: Makes things way fast and let's my outrun potential bacteria.
why do you have bacteria in the first place? i like secotoids thinking
There tends to be a little bacteria in most people's jars due to minor technique failings.
Quote:
Secotoid said: If you're willing to accept some contamination your approach is equally so,
elaborate... Not sure how taking an entire clean plate to a jar is risky...Quote:
Secotoid said:otherwise you would have to be absolutely certain your entire plates are entirely clean,
That's a standard everyone should live by
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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J. Jack Flash
stranger than ever.

Registered: 11/20/13
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Re: Cultivation General Discussion [Re: Psicomb]
#25949740 - 04/22/19 08:17 PM (4 years, 9 months ago) |
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i like splitting plates in half, and marking the jars in pairs. i feel like this can be useful for learning what's what. if both jars in a pair contaminate, when the rest don't, strong indication the contaminant was on the plate the whole time. combined with fotos, it helps to learn about seeing contamination in real life, instead of fotos on the forum.
shaperdreaming, my 'lab' is in a room with mold on the walls. contamination rate isn't zero, but it's tolerable. 15%, 20% max. have you considered every angle of drafts and such flowing thru your space? do you have carpet? check the pan under the fridge?
secotoid, are you by chance an engineer? cause you sound like an engineer. i approve.
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the j stands for jesus.2020 new years grow along
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tryptkaloids
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Re: Cultivation General Discussion [Re: Secotoid]
#25949743 - 04/22/19 08:18 PM (4 years, 9 months ago) |
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Quote:
Secotoid said: A master jar is a master culture. It is the one upon which all downstream culture in a series depends. Otherwise it's just some jar. I see what you mean though and I think our contention lays more in debate of risk mitigation vs time management. I err on the side of risk mitigation when an order of magnitude greater resources depend on my assumptions. Even taking time as a resource, my method costs far less in any case but the idea. Least harm is also key.
A master culture is kept on agar and can be gone back to to make more master jars. Agar is the risk mitigation... Not sure how using a sliver if a plate instead of the whole thing uses less resources... Doesnt really add up
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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Failboat
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Re: Cultivation General Discussion [Re: tryptkaloids]
#25949744 - 04/22/19 08:18 PM (4 years, 9 months ago) |
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Step 1 produce CLEAN culture plates...
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Secotoid
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Re: Cultivation General Discussion [Re: tryptkaloids]
#25949765 - 04/22/19 08:28 PM (4 years, 9 months ago) |
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That's a standard everyone should strive for and preferably achieve. Living by requires absolute foresight or an often lengthy and interdependent series of assumptions. I'm not fond of those. So we're back to the assumption of an absolute lack of risk, an acceptance or risk or an effort to mitigate it. What you do clearly works so if you are satisfied there then you'll get no argument from me. What I recommend also works, and as I feel I have demonstrated rationally, it strives for perfection realistically.
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tryptkaloids
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Re: Cultivation General Discussion [Re: Secotoid]
#25949778 - 04/22/19 08:34 PM (4 years, 9 months ago) |
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That's the part im lost on... Not sure how using a fraction of the culture strives for perfection any more than using the whole culture... I dont have to assume my plates are clean because i can see it.
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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Secotoid
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Re: Cultivation General Discussion [Re: tryptkaloids]
#25949792 - 04/22/19 08:42 PM (4 years, 9 months ago) |
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You seem to be presuming the entire interior of the plate - culture, media, surface and all - to be effectively homogeneous. Hopefully that is true - realistically it is sometimes not. Unless you'd like to tell me you have some meaningful period of zero contamination which will continue indefinitely and you think we can all join you then you are taking a risk which I am advocating mitigating.
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mushboy
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Re: Cultivation General Discussion [Re: Secotoid]
#25949812 - 04/22/19 08:53 PM (4 years, 9 months ago) |
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tryptkaloids
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Re: Cultivation General Discussion [Re: mushboy]
#25949880 - 04/22/19 09:34 PM (4 years, 9 months ago) |
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The thing is it's not a presumption because i can see if there's contams on my plate.
The only time it's a risk is when the culture looks off and in that case i just don't take it to grain. Because it's a risk.
It's common practice to use a full plate for inoculation. I think you'll be the one that has to convince people a grain of rice wedge to grain is worth it.
When i get contams in my grain (rarely) its from technique 98% of the time. The other 2% is when i say fuckit that's clean enough.
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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mushboy
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Re: Cultivation General Discussion [Re: tryptkaloids]
#25949882 - 04/22/19 09:36 PM (4 years, 9 months ago) |
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Quote:
tryptkaloids said: It's common practice to use a full plate for inoculation.
i dont use the full plate i cut out large wedges leaving the edges behind. for the same reason i dont inoculate with cultures that have grown to the edge of the plate. potential nasties might be lurking round them edges waiting to explode when they hit grains.
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tryptkaloids
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Re: Cultivation General Discussion [Re: tryptkaloids]
#25949885 - 04/22/19 09:38 PM (4 years, 9 months ago) |
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Quote:
tryptkaloids said: The thing is it's not a presumption because i can see if there's contams on my plate.
The Tiger drop, pasty's lc and most li teks depend on this and those are all tried and true
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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tryptkaloids
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Re: Cultivation General Discussion [Re: mushboy]
#25949888 - 04/22/19 09:39 PM (4 years, 9 months ago) |
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Paranoia mushboy
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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mushboy
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Re: Cultivation General Discussion [Re: tryptkaloids]
#25949891 - 04/22/19 09:43 PM (4 years, 9 months ago) |
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tiger dropping vs petri wwedge is a different ball game.
petris do have the slight vector of edge contamination if you are not careful. you have to really fuck up to do that with a miniround.
sooooo, with petris,, as a rule i do cut the wwedge out and leave the edges behind
too many times ive seen something weird grow from the edge of the wwedge/agar onto the grains. so i stop including the edges.
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muhfreedumb


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Re: Cultivation General Discussion [Re: tryptkaloids]
#25949902 - 04/22/19 09:47 PM (4 years, 9 months ago) |
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This exactly ^, for me using pasty plates: a whole plate is simpler, just dump it out, no scalpel, one movement. That's lower risk than cutting a wedge out. But if you aren't using pasty plates, maybe cutting a smaller bit is.
It seems like this thread (and many others) often devolves into competition over "best" methods, but this hobby doesn't even have a quantifiable standard of productivity (when's the last time you saw a psilocybin content by weight analysis?). If it works for you go ahead and tell people what you did and why it worked, and even better if it didn't work for you tell people why it didn't so they can learn from other's mistakes.
Edited by muhfreedumb (04/22/19 09:50 PM)
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tryptkaloids
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Re: Cultivation General Discussion [Re: mushboy]
#25949905 - 04/22/19 09:48 PM (4 years, 9 months ago) |
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Only kinda. Same rules apply. The edge "rule" seems more reasonable than using a sliver to colonize grain. That just seems like a massive waste of time
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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