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InvisibleBoogieman47
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Re: Cultivation General Discussion [Re: dmppb]
    #25941928 - 04/18/19 04:10 PM (4 years, 11 months ago)

Yeah a nickel gets you 10 per disc ... and do you mean the shiny halo around the mycelium? If so all that is in most circumstances uncolonized myc ... that's the leading edge for some cultures..


Yeah for 2 month old tuba that's good bacteria isnt always going to fuck up grows and I believe every grow is bacterial to a degree ... it's when the shit looks like over buttery popcorn is when you shoudm worry Haha


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OfflineLegend21
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Re: Cultivation General Discussion [Re: tryptkaloids]
    #25942072 - 04/18/19 05:37 PM (4 years, 11 months ago)

Quote:

tryptkaloids said:
Legend that plate will be fine to transfer from




Cool I am going to do some transfers tomorrow.

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OfflineCamera93
We got dicks like Jesus
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Registered: 08/15/18
Posts: 3,302
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Re: Cultivation General Discussion [Re: Legend21]
    #25942363 - 04/18/19 08:05 PM (4 years, 11 months ago)



They arrived, lids marked pp5 as well
Used 1.5 cups of grain soak agar for 10 plates
23qt presto can hold 24 with the way i have my bottom raised
8g agar agar 2 cups grain soak water

PC cycle starting now, ill letcha know if its a melted mess after


--------------------
All I need are some tasty waves, a cool buzz, and I’m fine.

Whatever you decide won’t really impact our survival
Close your eyes, and do the best that you can


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Offlineibuprofemin
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Re: Cultivation General Discussion [Re: Camera93]
    #25942369 - 04/18/19 08:08 PM (4 years, 11 months ago)

Quote:

Camera93 said:


They arrived, lids marked pp5 as well
Used 1.5 cups of grain soak agar for 10 plates
23qt presto can hold 24 with the way i have my bottom raised
8g agar agar 2 cups grain soak water

PC cycle starting now, ill letcha know if its a melted mess after




Are those from Redicontainer? I tried them and they all melted... let me know if it works!

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OfflineCamera93
We got dicks like Jesus
I'm a teapot


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Posts: 3,302
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Re: Cultivation General Discussion [Re: ibuprofemin]
    #25942405 - 04/18/19 08:31 PM (4 years, 11 months ago)

Quote:

Camera93 said:
what about these for no pours?

says its polypropylene which is the pp5 I'm looking for right?



Quote:

ibuprofemin said:
Quote:

Camera93 said:


They arrived, lids marked pp5 as well
Used 1.5 cups of grain soak agar for 10 plates
23qt presto can hold 24 with the way i have my bottom raised
8g agar agar 2 cups grain soak water

PC cycle starting now, ill letcha know if its a melted mess after




Are those from Redicontainer? I tried them and they all melted... let me know if it works!




Theres the link, they are from webstrauntstore and choice brand


--------------------
All I need are some tasty waves, a cool buzz, and I’m fine.

Whatever you decide won’t really impact our survival
Close your eyes, and do the best that you can


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OfflineCamera93
We got dicks like Jesus
I'm a teapot


Registered: 08/15/18
Posts: 3,302
Last seen: 4 hours, 12 minutes
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Re: Cultivation General Discussion [Re: Camera93]
    #25942508 - 04/18/19 09:19 PM (4 years, 11 months ago)

Out of the pc and happy to report no mess
A couple did warp a bit on the bottom, but lids didnt fall off or anything



--------------------
All I need are some tasty waves, a cool buzz, and I’m fine.

Whatever you decide won’t really impact our survival
Close your eyes, and do the best that you can


Edited by Camera93 (04/19/19 06:43 AM)

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Offlineteladi
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Registered: 06/27/17
Posts: 1,189
Loc: South Africa Flag
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Re: Cultivation General Discussion [Re: Camera93]
    #25943013 - 04/19/19 06:01 AM (4 years, 11 months ago)

On the subject of plastics that can withstand sterilization, only PP has a materials/resin identification code assigned. Also known as a recycling code.

1. PETE/PET
2. HDPE
3. PVC
4. LDPE
5. PP
6. PS
7 is a catch-all for other plastics, and is not necessarily always indicated.

Of this list, only PP can be sterilized in a PC. Everything else will warp or shrink. Others can also be used:

  • E-CTFE - Ethylene ChloroTriFluoroEthylene or Halar
  • ETFE - Ethylene tetrafluoroethylene
  • FEP - Fluorinated ethylene propylene
  • PC (No longer than 20 minutes) - Polycarbonate
  • PFA - Perfluoroalkoxy alkanes
  • PMP/TPX - Polymethylpentene
  • PP - Polypropylene
  • PTFE - Polytetrafluoroethylene or Teflon



China has a different set of codes: https://en.wikipedia.org/wiki/Recycling_codes#List_of_Chinese_codes_for_plastics_products

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Offlineibuprofemin
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Re: Cultivation General Discussion [Re: Camera93]
    #25943144 - 04/19/19 08:05 AM (4 years, 11 months ago)

Quote:

Camera93 said:
Quote:

Camera93 said:
what about these for no pours?

says its polypropylene which is the pp5 I'm looking for right?



Quote:

ibuprofemin said:
Quote:

Camera93 said:


They arrived, lids marked pp5 as well
Used 1.5 cups of grain soak agar for 10 plates
23qt presto can hold 24 with the way i have my bottom raised
8g agar agar 2 cups grain soak water

PC cycle starting now, ill letcha know if its a melted mess after




Are those from Redicontainer? I tried them and they all melted... let me know if it works!




Theres the link, they are from webstrauntstore and choice brand





Thanks for that! Will probably buy some at some point soon!

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OfflineLegend21
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Re: Cultivation General Discussion [Re: ibuprofemin]
    #25943339 - 04/19/19 10:24 AM (4 years, 11 months ago)

Why does my agar do this after PC everytime?


It’s  like everything separates. I put the powder into the media bottle, pour boiling water into it, make sure everything is completely dissolved, and then PC.

Everytime this flaking and nasty shit occurs. Never get contam but I assume the nutrients aren’t evenly dispersed.

20g lme 20g agar per liter. But even with 500ml bottles it does it at 10g/10g


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InvisiblemushboyMDiscord
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Posts: 32,666
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Re: Cultivation General Discussion [Re: Legend21]
    #25943345 - 04/19/19 10:25 AM (4 years, 11 months ago)

stop using boiling water just use regular room temp water then PC.

i use cold water, dump in 9g lme/agar per 400-500ml and pc. comes out like this.

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OfflineLegend21
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Re: Cultivation General Discussion [Re: mushboy]
    #25943362 - 04/19/19 10:31 AM (4 years, 11 months ago)

Quote:

mushboy said:
stop using boiling water just use regular room temp water then PC.

i use cold water, dump in 9g lme/agar per 400-500ml and pc. comes out like this.




Okay I’ll try that next time I thought you had to get it fully dissolved before doing the pc cycles. Do you just shake it up before putting it in the pc.

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InvisiblemushboyMDiscord
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Re: Cultivation General Discussion [Re: Legend21]
    #25943385 - 04/19/19 10:37 AM (4 years, 11 months ago)

yah i give the bottle a shake but you dont use boiling water with powders. it forms a 'skin' on the lme and makes those lumps.

ever try making a cake with hot water? same shit happens.

its also pointless to worry about dissolving the powder pre pc. its going to dissolve during the cycle.

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OfflineLegend21
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Re: Cultivation General Discussion [Re: mushboy]
    #25943410 - 04/19/19 10:46 AM (4 years, 11 months ago)

Quote:

mushboy said:
yah i give the bottle a shake but you dont use boiling water with powders. it forms a 'skin' on the lme and makes those lumps.

ever try making a cake with hot water? same shit happens.

its also pointless to worry about dissolving the powder pre pc. its going to dissolve during the cycle.





Alright cool thanks for the information. I guess I assumed you had to do that from the first time I did pasty plates but I guess that’s a whole different story since they’re no pours. Appreciate the help bro

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OfflineShaperDreaming
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Re: Cultivation General Discussion [Re: mushboy]
    #25943418 - 04/19/19 10:49 AM (4 years, 11 months ago)

Quote:

mushboy said:
ever try making a cake with hot water? same shit happens.



I knew you were a homemaker Mushboy, fucking knew it.

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InvisiblemushboyMDiscord
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Re: Cultivation General Discussion [Re: Legend21]
    #25943422 - 04/19/19 10:51 AM (4 years, 11 months ago)

you boil the potato flakes because you need to extract the nutes into the water/dissolve the large flakes. if it were powder potato you could skip the boiling.

in fact if you cold soaked the flakes it would also work but whos got time to soak potato flakes for agar:lol:


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InvisibleYesum
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Re: Cultivation General Discussion [Re: Legend21]
    #25943449 - 04/19/19 11:01 AM (4 years, 11 months ago)

Quote:

Legend21 said:
Why does my agar do this after PC everytime?


It’s  like everything separates. I put the powder into the media bottle, pour boiling water into it, make sure everything is completely dissolved, and then PC.

Everytime this flaking and nasty shit occurs. Never get contam but I assume the nutrients aren’t evenly dispersed.

20g lme 20g agar per liter. But even with 500ml bottles it does it at 10g/10g






Are u going over 15psi maybe. :shrug:


--------------------

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InvisibleBoogieman47
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Re: Cultivation General Discussion [Re: Yesum]
    #25943466 - 04/19/19 11:09 AM (4 years, 11 months ago)

I dont think he is mixing it before putting it in .. probably thought the it would boil and mix on its own.. that malt is sticky


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InvisiblemushboyMDiscord
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Re: Cultivation General Discussion [Re: Boogieman47]
    #25943477 - 04/19/19 11:13 AM (4 years, 11 months ago)

sticky because the boiling wateer he used cooked the powder into clumps. preventing it from dissolving.

thats why you use ice cold water to make super clear lme agar :wink:

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OfflineLegend21
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Re: Cultivation General Discussion [Re: Yesum]
    #25943489 - 04/19/19 11:19 AM (4 years, 11 months ago)

Quote:

Boogieman47 said:
I dont think he is mixing it before putting it in .. probably thought the it would boil and mix on its own.. that malt is sticky




Yeah I figured by pouring boiling water in the media bottle and shaking the bottle that it would mix it.

Quote:

Yesum said:
Quote:

Legend21 said:
Why does my agar do this after PC everytime?


It’s  like everything separates. I put the powder into the media bottle, pour boiling water into it, make sure everything is completely dissolved, and then PC.

Everytime this flaking and nasty shit occurs. Never get contam but I assume the nutrients aren’t evenly dispersed.

20g lme 20g agar per liter. But even with 500ml bottles it does it at 10g/10g






Are u going over 15psi maybe. :shrug:




Just 15 psi 25 minutes

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InvisibleBoogieman47
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Re: Cultivation General Discussion [Re: mushboy] * 3
    #25943491 - 04/19/19 11:19 AM (4 years, 11 months ago)

I like mine cloudy and hard to see through thank you


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