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ibuprofemin
Stranger
Registered: 04/03/19
Posts: 151
Last seen: 3 years, 6 months
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Re: Cultivation General Discussion [Re: Yesum]
#25931117 - 04/12/19 06:03 PM (4 years, 11 months ago) |
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Is it ok to use a sterile q-tip to streak spore solution into an agar plate?
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Yesum
Furry as Fuc
Registered: 11/05/12
Posts: 13,124
Loc: Central Part of Town
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Re: Cultivation General Discussion [Re: ibuprofemin]
#25931123 - 04/12/19 06:05 PM (4 years, 11 months ago) |
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Quote:
ibuprofemin said: Is it ok to use a sterile q-tip to streak spore solution into an agar plate?
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ibuprofemin
Stranger
Registered: 04/03/19
Posts: 151
Last seen: 3 years, 6 months
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Re: Cultivation General Discussion [Re: Yesum]
#25931140 - 04/12/19 06:16 PM (4 years, 11 months ago) |
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Quote:
Yesum said:
Quote:
ibuprofemin said: Is it ok to use a sterile q-tip to streak spore solution into an agar plate?
Perfect! Follow up, are you guys just recapping the spore syringe and storing again for later use? With 12 ccs of fluid and only needing a drop, seems like a single syringe would last forever.
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tryptkaloids
Learner
Registered: 02/08/15
Posts: 12,650
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Re: Cultivation General Discussion [Re: ibuprofemin]
#25931143 - 04/12/19 06:20 PM (4 years, 11 months ago) |
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Yes. Syringes are made for cakes so it spreads much further with agar
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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ibuprofemin
Stranger
Registered: 04/03/19
Posts: 151
Last seen: 3 years, 6 months
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Re: Cultivation General Discussion [Re: tryptkaloids]
#25931169 - 04/12/19 06:37 PM (4 years, 11 months ago) |
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Quote:
tryptkaloids said: Yes. Syringes are made for cakes so it spreads much further with agar
Awesome. Have access to sterile caps too, so thatβll work nice!
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FishLevelMidnight
Aquaman
Registered: 09/01/17
Posts: 2,328
Last seen: 7 months, 22 days
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Re: Cultivation General Discussion [Re: Yesum]
#25931184 - 04/12/19 06:52 PM (4 years, 11 months ago) |
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Quote:
Yesum said: Eat has some well written teks on oven pasteurization
Iβm lucky enough to be in a spot with a digital oven.
I set that baby to 150F and put mycobags of bulk and casing in there for 2 hours and it works soooooo good and easy. Love it.
-------------------- Trade List
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Yesum
Furry as Fuc
Registered: 11/05/12
Posts: 13,124
Loc: Central Part of Town
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Have you checked to see if the oven temp is accurate? But hell yeah. That's cheating.
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JHOVA
Post whore
Registered: 02/17/17
Posts: 4,727
Loc:
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Re: Cultivation General Discussion [Re: Yesum]
#25931203 - 04/12/19 06:58 PM (4 years, 11 months ago) |
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I think eats preheats his sub in a bucket first then loads it hot. His tek states it takes a long time to reach the right temp when starting at room temp.
-------------------- π
π΄ π° πΌ π² π» πΈ π½ πΆ π
π
π° πΏ
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LtLurker
Lost Sailor
Registered: 01/03/18
Posts: 7,535
Loc: Borderlands
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Re: Cultivation General Discussion [Re: JHOVA]
#25931216 - 04/12/19 07:03 PM (4 years, 11 months ago) |
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Quote:
JHOVA said: I think eats preheats his sub in a bucket first then loads it hot. His tek states it takes a long time to reach the right temp when starting at room temp.
Trying this now. Added water and bulk into metal pot then straight into oven for couple hours @170. Should be much more efficient than small amounts as i need it for 4-5hrs at a time.
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juniperus
Frim Fram Sauce
Registered: 12/02/18
Posts: 718
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Re: Cultivation General Discussion [Re: bodhisatta]
#25931280 - 04/12/19 07:34 PM (4 years, 11 months ago) |
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Quote:
bodhisatta said: Funny i Don't think ive ever used a shot glass to do that. Probably wrote it that way to help noobs along. Squirt right on to the loop
Change that TEK dude , definitely did it that way at first based on your write-up. Totally unnecessary.
-------------------- To avoid all evil, to cultivate good, and to cleanse one's mind - this is the teaching of the Buddhas
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tryptkaloids
Learner
Registered: 02/08/15
Posts: 12,650
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Last seen: 14 hours, 16 minutes
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Quote:
fishermansjc said:
Quote:
Yesum said: Eat has some well written teks on oven pasteurization
Iβm lucky enough to be in a spot with a digital oven.
I set that baby to 150F and put mycobags of bulk and casing in there for 2 hours and it works soooooo good and easy. Love it.
I do too, but it only goes down to 170. I was in a hurry abd turned that shit to 400 to rush things and didnt turn it off fast enough
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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Enkidu
"No-Such-Person"
Registered: 07/09/16
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Re: Cultivation General Discussion [Re: tryptkaloids]
#25931891 - 04/13/19 06:38 AM (4 years, 11 months ago) |
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Posted this multiple places but goong out of town jn a few hours so truing to find out if this trich and what to do before I leave
-------------------- Within You , Without You
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tryptkaloids
Learner
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Re: Cultivation General Discussion [Re: Enkidu]
#25932256 - 04/13/19 10:35 AM (4 years, 11 months ago) |
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It is, toss it
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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Psicomb
shroom or die
Registered: 01/13/18
Posts: 4,749
Loc: the womb
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Re: Cultivation General Discussion [Re: tryptkaloids]
#25932296 - 04/13/19 10:58 AM (4 years, 11 months ago) |
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Yessah, that is mold
-------------------- When we constantly pull things apart trying to see how it works, we may end up with only an understanding of how to destroy something - nick sand
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Failboat
Fuck Up
Registered: 02/01/18
Posts: 8,736
Last seen: 1 day, 35 minutes
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Re: Cultivation General Discussion [Re: Psicomb]
#25932548 - 04/13/19 01:54 PM (4 years, 11 months ago) |
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I have 30qt of bacterial spawn that's mostly colonized. I figure instead of wasting coir I will spawn them to a bed covered with nice fine mulch inside of a barn with a dirt floor. Should have enough ambient light to help the cycle, but more than enough coverage to regulate temp and humidity.
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Yesum
Furry as Fuc
Registered: 11/05/12
Posts: 13,124
Loc: Central Part of Town
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Re: Cultivation General Discussion [Re: Failboat]
#25932645 - 04/13/19 02:56 PM (4 years, 11 months ago) |
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Quote:
Quirkmeister92 said: I have 30qt of bacterial spawn that's mostly colonized. I figure instead of wasting coir I will spawn them to a bed covered with nice fine mulch inside of a barn with a dirt floor. Should have enough ambient light to help the cycle, but more than enough coverage to regulate temp and humidity.
Or.
Just case the grain with coir. Shit fruites quick too. But u gotta use shoeboxes or trays or something like that. But it works.
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Nobler Hino
a dojo and a forge?!
Registered: 08/29/15
Posts: 1,780
Loc: Deep Ellum
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Quote:
fishermansjc said:
Quote:
Yesum said: Eat has some well written teks on oven pasteurization
Iβm lucky enough to be in a spot with a digital oven.
I set that baby to 150F and put mycobags of bulk and casing in there for 2 hours and it works soooooo good and easy. Love it.
That does sound awesome. Do you check the core temp at all?
-------------------- "The sacred mushroom takes me by the hand and brings me to the world where everything is known. It is they, the sacred mushrooms, that speak in a way I can understand. I ask them and they answer me.β β Maria Sabina
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ibuprofemin
Stranger
Registered: 04/03/19
Posts: 151
Last seen: 3 years, 6 months
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Re: Cultivation General Discussion [Re: Nobler Hino]
#25932700 - 04/13/19 03:28 PM (4 years, 11 months ago) |
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Assuming that green/grayish spot is contamination... anything to do about it? Good thing there are some good fruits to clone to agar so I can have clean spawn, this was from an Ms syringe.
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Failboat
Fuck Up
Registered: 02/01/18
Posts: 8,736
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Re: Cultivation General Discussion [Re: Yesum]
#25932708 - 04/13/19 03:36 PM (4 years, 11 months ago) |
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Quote:
Yesum said:
Quote:
Quirkmeister92 said: I have 30qt of bacterial spawn that's mostly colonized. I figure instead of wasting coir I will spawn them to a bed covered with nice fine mulch inside of a barn with a dirt floor. Should have enough ambient light to help the cycle, but more than enough coverage to regulate temp and humidity.
Or.
Just case the grain with coir. Shit fruites quick too. But u gotta use shoeboxes or trays or something like that. But it works.
Wish I saw this first hah oh well
Edited by Failboat (04/13/19 03:37 PM)
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tryptkaloids
Learner
Registered: 02/08/15
Posts: 12,650
Loc: Exact Center
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Re: Cultivation General Discussion [Re: Boogieman47]
#25932719 - 04/13/19 03:45 PM (4 years, 11 months ago) |
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Quote:
Boogieman47 said: Yeah you can mix it back with regular shit and it be fine again ..
How long should I let them sit?
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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