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Offlineicetech
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Re: Cultivation General Discussion [Re: Leftfield420]
    #25926222 - 04/10/19 05:51 AM (4 years, 9 months ago)

Psst... Love you guys..


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OfflineEl Chupacabra
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Re: Cultivation General Discussion [Re: LogicaL Chaos]
    #25926240 - 04/10/19 06:18 AM (4 years, 9 months ago)

Quote:

bodhisatta said:
Why in the world do people put spore solution directly to agar :shrug:

Bacteria causes bacteria not moisture.



Quote:

LogicaL Chaos said:
Wait, i thought putting spore solution on agar was standard procedure?




My best guess is that he knows it's a PITA to drop a single drop onto agar and he knows more than a drop = potential problems.
I think bod favors using a sterile shot glass with some spore solution and a loop.
IDK but if I had to work with a spore syringe I would probably do the same.


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OfflineFailboat
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Re: Cultivation General Discussion [Re: El Chupacabra]
    #25926245 - 04/10/19 06:21 AM (4 years, 9 months ago)

I find without a sharp it is very easy to get one drop on a plate.


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InvisiblePsicomb
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Re: Cultivation General Discussion [Re: Failboat]
    #25926262 - 04/10/19 06:38 AM (4 years, 9 months ago)

Spore solution to agar has never caused me any issues :shrug:


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When we constantly pull things apart trying to see how it works, we may end up with only an understanding of how to destroy something
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InvisiblebodhisattaMDiscordReddit
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Re: Cultivation General Discussion [Re: Psicomb] * 1
    #25926305 - 04/10/19 07:27 AM (4 years, 9 months ago)

Here is what a fraction of a single drop, or barely touching a print is capable of on agar



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OfflineLegend21
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Re: Cultivation General Discussion [Re: bodhisatta]
    #25926378 - 04/10/19 08:15 AM (4 years, 9 months ago)

Quote:

bodhisatta said:
Here is what a fraction of a single drop, or barely touching a print is capable of on agar






So if using a syringe how do you go from spore solution to agar? With a loop?
I’m sure you just work from prints at this point just curious. I’m having shit luck germinating spore syringes to regular plates. I don’t end up with bacteria I just end up with nothing at all lol


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InvisibleYesum
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Re: Cultivation General Discussion [Re: LogicaL Chaos]
    #25926477 - 04/10/19 09:00 AM (4 years, 9 months ago)

Quote:

LogicaL Chaos said:
Wait, i thought putting spore solution on agar was standard procedure?





It is


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InvisiblebodhisattaMDiscordReddit
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Re: Cultivation General Discussion [Re: Yesum]
    #25926487 - 04/10/19 09:04 AM (4 years, 9 months ago)

Spread plate with inoculation loop is standard procedure for liquids to agar. Some people do a drop right on a plate. I think its horrible practice


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Offlinetryptkaloids
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Re: Cultivation General Discussion [Re: bodhisatta]
    #25926493 - 04/10/19 09:08 AM (4 years, 9 months ago)

Why tho? Shot glass and loop are 2 extra vectors


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"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
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Learn the tried and true way to grow mushrooms
Use the Damn search engine
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Pick a book, Make some chips!
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Offlinegizmodo
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Re: Cultivation General Discussion [Re: bodhisatta]
    #25926518 - 04/10/19 09:25 AM (4 years, 9 months ago)

Quote:

bodhisatta said:
Here is what a fraction of a single drop, or barely touching a print is capable of on agar




What kind of scope you using? :evil2:


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Offlinepinedownpioneer
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Re: Cultivation General Discussion [Re: gizmodo]
    #25926534 - 04/10/19 09:32 AM (4 years, 9 months ago)

Harvested some fungi today:feelsgoodman:


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Offlinepinedownpioneer
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Re: Cultivation General Discussion [Re: pinedownpioneer]
    #25926536 - 04/10/19 09:33 AM (4 years, 9 months ago)

Anyone ate the β€˜mars’ Cubes?


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InvisibleCaps McGee
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Re: Cultivation General Discussion [Re: pinedownpioneer]
    #25926554 - 04/10/19 09:42 AM (4 years, 9 months ago)

MS potency will vary, even among fruit from the same box


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Offlinetryptkaloids
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Re: Cultivation General Discussion [Re: Caps McGee]
    #25929435 - 04/11/19 07:02 PM (4 years, 9 months ago)

I'm mixin' poo and reading old teks. I've always added verm for texture, but older teks just use straight pasteurized poo. Is fluffing it up uneccesary?


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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Offlinedmppb
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Re: Cultivation General Discussion [Re: tryptkaloids]
    #25929577 - 04/11/19 08:28 PM (4 years, 9 months ago)

Quote:

tryptkaloids said:
I'm mixin' poo and reading old teks. I've always added verm for texture, but older teks just use straight pasteurized poo. Is fluffing it up uneccesary?




i used it for a while ,what i did was i passed it through my straw shredder and it comes out like fine dirt , you can use a blender with water i did that too, then drain and add verm to adjust moisture , its kinda is fluffy after the shred


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InvisiblebodhisattaMDiscordReddit
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Re: Cultivation General Discussion [Re: gizmodo]
    #25929903 - 04/12/19 04:57 AM (4 years, 9 months ago)

Quote:

gizmodo said:
Quote:

bodhisatta said:
Here is what a fraction of a single drop, or barely touching a print is capable of on agar




What kind of scope you using? :evil2:



120$ amscope


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InvisiblebodhisattaMDiscordReddit
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Re: Cultivation General Discussion [Re: tryptkaloids]
    #25929904 - 04/12/19 04:57 AM (4 years, 9 months ago)

Quote:

tryptkaloids said:
Why tho? Shot glass and loop are 2 extra vectors



What's the shot glass for? The spores are the vector, more on the plate = worse time. A loop is flamed


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OfflineEl Chupacabra
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Re: Cultivation General Discussion [Re: bodhisatta]
    #25930029 - 04/12/19 06:40 AM (4 years, 9 months ago)

Quote:

bodhisatta said:
What's the shot glass for? The spores are the vector, more on the plate = worse time. A loop is flamed



For dipping apparently.

Quote:

bodhisatta said:
Have a sterilized shot glass or a blank petri dish(inside is sterile still)
flame your needle on the spore syringe then move into the SAB
squirt out a drop to cool the needle and then squirt a little bit into your dish
take your inoculation loop (I use disposable plastic ones) otherwise flame it
bring into the SAB then dip in agar(if flamed) then dip in the spore solution in the dish
then make a Z pattern on the dish with the inoculation loop




Sounds good to me.:thumbup:


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Offlinelordfurd
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Re: Cultivation General Discussion [Re: El Chupacabra]
    #25930061 - 04/12/19 07:13 AM (4 years, 9 months ago)

Say you harvest a shoebox by floating the substrate to the top in water (bods harvest Tek), would the side/bottom pins lose much potency if left for too long harvesting the top since they are water soluble?


Edited by lordfurd (04/12/19 07:19 AM)


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InvisiblebodhisattaMDiscordReddit
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Re: Cultivation General Discussion [Re: lordfurd] * 1
    #25930075 - 04/12/19 07:21 AM (4 years, 9 months ago)

Funny i Don't think ive ever used a shot glass to do that. Probably wrote it that way to help noobs along. Squirt right on to the loop :shrug:


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