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InvisibleCaps McGee
Grandaddy Smurfshack
I'm a teapot User Gallery


Registered: 10/28/17
Posts: 14,357
Loc: ally known as ... Flag
Re: Cultivation General Discussion [Re: ibuprofemin]
    #25924788 - 04/09/19 10:50 AM (4 years, 11 months ago)

Quote:

ibuprofemin said:
Basically just did filter disc and metal ring. No metal lid.



Your jars only need molecular gas exchange, which can occur in even a loose unmodded lid ... I wouldnt suggest skipping the lid, as the filter alone will likely not do well against the shake... you can drill a hole and trim SFD's into nickel sized pads... get more out of each filter, and maintain the strength of the lid

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OfflineLegend21
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Re: Cultivation General Discussion [Re: Caps McGee]
    #25924793 - 04/09/19 10:53 AM (4 years, 11 months ago)

I decided to G2G that chunky thick jar just too see what happens. I made a ton of jars to early and didn’t want them sitting around. Let’s see what happens :rofl:

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Offlineibuprofemin
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Re: Cultivation General Discussion [Re: Caps McGee]
    #25924794 - 04/09/19 10:54 AM (4 years, 11 months ago)

Quote:

Caps McGee said:
Quote:

ibuprofemin said:
Basically just did filter disc and metal ring. No metal lid.



Your jars only need molecular gas exchange, which can occur in even a loose unmodded lid ... I wouldnt suggest skipping the lid, as the filter alone will likely not do well against the shake... you can drill a hole and trim SFD's into nickel sized pads... get more out of each filter, and maintain the strength of the lid




Ahh ok. So wouldn’t really be at too much of a risk of drying out then? I see what you’re saying about the shake. For next batch will get the metal lid back on!

These jars are about 3-4 days in, still patiently waiting. Forgot to inject on the side so it may be a while till I see anything.

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Offlinetryptkaloids
Learner
I'm a teapot


Registered: 02/08/15
Posts: 12,650
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Re: Cultivation General Discussion [Re: ibuprofemin]
    #25924815 - 04/09/19 11:07 AM (4 years, 11 months ago)

Quote:

ibuprofemin said:
Quote:

Caps McGee said:
Quote:

ibuprofemin said:
Basically just did filter disc and metal ring. No metal lid.



Your jars only need molecular gas exchange, which can occur in even a loose unmodded lid ... I wouldnt suggest skipping the lid, as the filter alone will likely not do well against the shake... you can drill a hole and trim SFD's into nickel sized pads... get more out of each filter, and maintain the strength of the lid




Ahh ok. So wouldn’t really be at too much of a risk of drying out then? I see what you’re saying about the shake. For next batch will get the metal lid back on!

These jars are about 3-4 days in, still patiently waiting. Forgot to inject on the side so it may be a while till I see anything.



Start agar yesterday. Spore to grain sucks for many reasons.  Those jars may dry out before they even germinate. They definitely wont colonize fully. Even when we do use full sfds (which I do all the time) we still put the metal lid with a hole in it under the filter. Ive had
Jars dry out when the hole is just barely too big. With the entire jar opening essentially open to air it wont retain the moisture it needs


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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OfflineShaperDreaming
Weirdo
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Registered: 10/30/18
Posts: 3,429
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Re: Cultivation General Discussion [Re: mushboy]
    #25924817 - 04/09/19 11:07 AM (4 years, 11 months ago)

Quote:

mushboy said:
if you are going to leave tthe lid on just fruit it like normal. save the marta space for something that needs it



Well, for now I don't have a lot of good shelving space that has lights set up, so I'm mostly using it for that as all I have set up for mycology right now (and for likely a month-ish) is my martha, hence the questions. You may remember my heating issues when I moved, which have now settled, but I've learned that the space I built out for mushrooms will be too challenging to regulate effectively, so I have to plan something else.

Basically, it's a temporary solution that I need to store my shoeboxes in my martha for about a month and I'm wondering how best to set it up for cubes (lids on or off).

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Offlineibuprofemin
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Registered: 04/03/19
Posts: 151
Last seen: 3 years, 6 months
Re: Cultivation General Discussion [Re: tryptkaloids]
    #25924823 - 04/09/19 11:12 AM (4 years, 11 months ago)

Quote:

tryptkaloids said:
Quote:

ibuprofemin said:
Quote:

Caps McGee said:
Quote:

ibuprofemin said:
Basically just did filter disc and metal ring. No metal lid.



Your jars only need molecular gas exchange, which can occur in even a loose unmodded lid ... I wouldnt suggest skipping the lid, as the filter alone will likely not do well against the shake... you can drill a hole and trim SFD's into nickel sized pads... get more out of each filter, and maintain the strength of the lid




Ahh ok. So wouldn’t really be at too much of a risk of drying out then? I see what you’re saying about the shake. For next batch will get the metal lid back on!

These jars are about 3-4 days in, still patiently waiting. Forgot to inject on the side so it may be a while till I see anything.



Start agar yesterday. Spore to grain sucks for many reasons.  Those jars may dry out before they even germinate. They definitely wont colonize fully. Even when we do use full sfds (which I do all the time) we still put the metal lid with a hole in it under the filter. Ive had
Jars dry out when the hole is just barely too big. With the entire jar opening essentially open to air it wont retain the moisture it needs




Waiting on my first flush to come in then I’ll be cloning some to agar and going from there’s. No more syringes for me after this hopefully. Just had an extra and wanted to see what working w grain was like.  Guess we’ll see what happens w these jars while I wait on agar stuff.

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Offlinetryptkaloids
Learner
I'm a teapot


Registered: 02/08/15
Posts: 12,650
Loc: Exact Center
Last seen: 12 hours, 15 minutes
Trusted Cultivator
Re: Cultivation General Discussion [Re: ShaperDreaming]
    #25924846 - 04/09/19 11:25 AM (4 years, 11 months ago)

Quote:

ShaperDreaming said:
I'm wondering how best to set it up for cubes (lids on or off).



Ive never used a Martha, especially for cubes, but if i did i would either do it without the lid or just birth the shoebox and set it on the Martha shelf and reuse the shoebox before firsr flush


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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OfflineLtLurker
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Registered: 01/03/18
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Re: Cultivation General Discussion [Re: tryptkaloids]
    #25924852 - 04/09/19 11:27 AM (4 years, 11 months ago)

:whathesaid:
Lid on would kinda defeat the purpose of setting up a controlled environment. If you're dialed in right, you shouldn't need a lid to maintain higher humidity than the ambient surroundings.

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OfflineShaperDreaming
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Re: Cultivation General Discussion [Re: LtLurker]
    #25924917 - 04/09/19 12:17 PM (4 years, 11 months ago)

Cool cool, thanks ya'll. It's not my favorite situation, just trying to do right by my mush.

Actually, maybe I can set up a little bench right next to the martha and just get the light from being close enough.

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Invisiblesh4d0ws
LSx
I'm a teapot User Gallery

Registered: 02/26/08
Posts: 12,086
Re: Cultivation General Discussion [Re: Caps McGee]
    #25924983 - 04/09/19 12:50 PM (4 years, 11 months ago)

Quote:

Caps McGee said:
Quote:

ibuprofemin said:
Basically just did filter disc and metal ring. No metal lid.



Your jars only need molecular gas exchange, which can occur in even a loose unmodded lid ... I wouldnt suggest skipping the lid, as the filter alone will likely not do well against the shake... you can drill a hole and trim SFD's into nickel sized pads... get more out of each filter, and maintain the strength of the lid





Don't think the grain will do anything to the filter (during shaking or whatever). People have used SFDs like that plenty of times. It's probably possible it could dry right out but I bet if it colonized within a reasonable time frame it would probably alright.


--------------------

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Offlinetryptkaloids
Learner
I'm a teapot


Registered: 02/08/15
Posts: 12,650
Loc: Exact Center
Last seen: 12 hours, 15 minutes
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Re: Cultivation General Discussion [Re: sh4d0ws]
    #25925020 - 04/09/19 01:09 PM (4 years, 11 months ago)

Not likely to happen with spore solition imo


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

Extras: Filter Print Post Top
Offlineibuprofemin
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Registered: 04/03/19
Posts: 151
Last seen: 3 years, 6 months
Re: Cultivation General Discussion [Re: tryptkaloids]
    #25925045 - 04/09/19 01:24 PM (4 years, 11 months ago)

Quote:

tryptkaloids said:
Not likely to happen with spore solition imo





Figure I’ll know by the time I have some clones isolated... have everything for that ready to go just waiting on fruits. In meantime, will order more SFDs and get the metal lids drilled with a couple small holes. Figure since I’ll be going from agar now won’t need to bother with injection ports for them. And then by the time I see what shakes out with these jars, I should have some agar isolates ready to go. Also saved a little from that syringe I used for the grain jars to squirt a couple drops on the plates and see what happens there!

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Offlinedmppb
On course


Registered: 11/11/11
Posts: 1,043
Loc: universe
Last seen: 16 days, 16 hours
Re: Cultivation General Discussion [Re: ibuprofemin]
    #25925683 - 04/09/19 07:32 PM (4 years, 11 months ago)

Quote:

ibuprofemin said:
Quote:

tryptkaloids said:
Not likely to happen with spore solition imo





Figure I’ll know by the time I have some clones isolated... have everything for that ready to go just waiting on fruits. In meantime, will order more SFDs and get the metal lids drilled with a couple small holes. Figure since I’ll be going from agar now won’t need to bother with injection ports for them. And then by the time I see what shakes out with these jars, I should have some agar isolates ready to go. Also saved a little from that syringe I used for the grain jars to squirt a couple drops on the plates and see what happens there!




i think the term isolating goes more for MS sectors , when you clone you narrow down the genetics enough to just test the culture and not have to do more isolating , am i right ?

also , when you drop spores from syringe to agar , get the smallest , and i mean SMALLEST tiny mini almost invisible drop , that'll do it , one big drop can ruin the moisture in there and cause bacteria , just my experience.


--------------------
...and when you loose control ; you'll reap the harvest you have sown.

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Offlinetryptkaloids
Learner
I'm a teapot


Registered: 02/08/15
Posts: 12,650
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Re: Cultivation General Discussion [Re: dmppb]
    #25925730 - 04/09/19 08:00 PM (4 years, 11 months ago)

There's no merit to that


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

Extras: Filter Print Post Top
Offlinedmppb
On course


Registered: 11/11/11
Posts: 1,043
Loc: universe
Last seen: 16 days, 16 hours
Re: Cultivation General Discussion [Re: tryptkaloids]
    #25925734 - 04/09/19 08:03 PM (4 years, 11 months ago)

Quote:

tryptkaloids said:
There's no merit to that




no understando , no merit to what?


--------------------
...and when you loose control ; you'll reap the harvest you have sown.

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Offlinetryptkaloids
Learner
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Registered: 02/08/15
Posts: 12,650
Loc: Exact Center
Last seen: 12 hours, 15 minutes
Trusted Cultivator
Re: Cultivation General Discussion [Re: dmppb]
    #25925905 - 04/09/19 10:15 PM (4 years, 11 months ago)

Excess moisture causing bacteria it may spread it.


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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InvisibleYesum
Furry as Fuc
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Registered: 11/05/12
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Re: Cultivation General Discussion [Re: tryptkaloids]
    #25926048 - 04/10/19 01:44 AM (4 years, 11 months ago)

Yeah. You should see my germ plates. I try for 1 drop put usually shoot out a lot more. Just gives me more inoculation points.


--------------------

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InvisiblebodhisattaMDiscordReddit
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Re: Cultivation General Discussion [Re: Yesum]
    #25926193 - 04/10/19 05:09 AM (4 years, 11 months ago)

Why in the world do people put spore solution directly to agar :shrug:

Bacteria causes bacteria not moisture.

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OfflineLogicaL ChaosM
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Re: Cultivation General Discussion [Re: bodhisatta]
    #25926215 - 04/10/19 05:41 AM (4 years, 11 months ago)

Wait, i thought putting spore solution on agar was standard procedure?

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InvisibleLeftfield420
bong toker
Male


Registered: 02/26/16
Posts: 10,068
Re: Cultivation General Discussion [Re: LogicaL Chaos]
    #25926218 - 04/10/19 05:44 AM (4 years, 11 months ago)

Right...1 drop does go a long way..

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