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Offlineteladi
FUNKSOULBROTHER

Registered: 06/27/17
Posts: 1,189
Loc: South Africa Flag
Last seen: 5 months, 22 days
Re: Cultivation General Discussion [Re: icetech]
    #25833597 - 02/23/19 10:12 PM (4 years, 11 months ago)

Quote:

icetech said:
I haven't read up a ton on using LC, what is it about it that makes you stick with it? I have seen it colonized crazy fast, is it much harder to make than just doing agar?






Two reasons why I like LC:

1. My LC bottles are smaller than my grain jars. At about 200 ml of LC when colonized, that is enough for 10-20 grain jars. A grain master can inoculate less than that.
2. LC stores really well.

Some reasons why I don't like LC:

1. It is actually slower than A2G. You are adding a month long step between agar and grain. I figure it adds an extra two weeks between agar and bulk.
2. Contamination is amplified. Anything bad in your culture is made much worse.
3. If using a filter, you cannot get it wet or else you will have contamination grow through the filter.
4. Leftover LC can't or shouldn't be kept. It feels like a waste.

Using LC to inoculate BRF cakes gives fantastic results, and many more flushes than expected. Colonization is insanely quick compared to a spore syringe.


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InvisibleCaps McGee
Grandaddy Smurfshack
I'm a teapot User Gallery


Registered: 10/28/17
Posts: 14,357
Loc: ally known as ... Flag
Re: Cultivation General Discussion [Re: teladi]
    #25833675 - 02/23/19 10:59 PM (4 years, 11 months ago)

I quit using iso all together and still in the mid 90's... :shrug:

Mild soap(I use dawn) and warm water for tubs, antibacterial soap and warm water for my hands... when I wash them... lol
:horrified:


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Offlinemushpunx
Fungus Punk
 User Gallery


Registered: 04/20/14
Posts: 13,394
Last seen: 11 days, 20 hours
Trusted Cultivator
Re: Cultivation General Discussion [Re: Caps McGee]
    #25833699 - 02/23/19 11:14 PM (4 years, 11 months ago)

I have a wash bottle of ISO I use to rinse stuff in front of my FH but as far as tubs go I just wash them with dish soap and water.


--------------------

Amateur Mycologists United
AMU Q&A


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Offlinemushpunx
Fungus Punk
 User Gallery


Registered: 04/20/14
Posts: 13,394
Last seen: 11 days, 20 hours
Trusted Cultivator
Re: Cultivation General Discussion [Re: teladi]
    #25833727 - 02/23/19 11:34 PM (4 years, 11 months ago)

Quote:

teladi said:
Quote:

icetech said:
I haven't read up a ton on using LC, what is it about it that makes you stick with it? I have seen it colonized crazy fast, is it much harder to make than just doing agar?






Two reasons why I like LC:

1. My LC bottles are smaller than my grain jars. At about 200 ml of LC when colonized, that is enough for 10-20 grain jars. A grain master can inoculate less than that.
2. LC stores really well.

Some reasons why I don't like LC:

1. It is actually slower than A2G. You are adding a month long step between agar and grain. I figure it adds an extra two weeks between agar and bulk.
2. Contamination is amplified. Anything bad in your culture is made much worse.
3. If using a filter, you cannot get it wet or else you will have contamination grow through the filter.
4. Leftover LC can't or shouldn't be kept. It feels like a waste.

Using LC to inoculate BRF cakes gives fantastic results, and many more flushes than expected. Colonization is insanely quick compared to a spore syringe.







To make LC, I blend my agar wedge in water in a sterilized Eberbach unit and the pour into my LC broth (in an Erlenmyer flask, I use ones with threaded caps with hole/SFD, or if it's unthreaded I stuff the top with poly fill), then I set it on a stir plate. The LC is typically ready in a few days to one week max.

LC is much faster if you blend up a wedge and then spin it in LC broth

I use G2G mostly though. And for a lot of things I just pour LI right from the Eberbach unit


Edited by mushpunx (02/24/19 12:28 AM)


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Offlineteladi
FUNKSOULBROTHER

Registered: 06/27/17
Posts: 1,189
Loc: South Africa Flag
Last seen: 5 months, 22 days
Re: Cultivation General Discussion [Re: mushpunx]
    #25833843 - 02/24/19 02:24 AM (4 years, 10 months ago)

Hmmmm...now you have me thinking. I have a glass blender with rubberized lid. Not quite an Eberbach, but it should work to be sterilized.

I normally do the Josex poke, which has served me well. I don't mind the time it takes, as I have many batches in different stages.

I have a shaker table in build progress that has taken me forever. That should decrease colonization time.


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OfflineThedillestpickle
cultured
Male


Registered: 02/02/16
Posts: 1,170
Loc: Canada Flag
Last seen: 3 years, 7 months
Re: Cultivation General Discussion [Re: teladi]
    #25834065 - 02/24/19 07:41 AM (4 years, 10 months ago)

I find it funny to see you guys putting so much into cleaning your tubs.  I literally just put my tubs in the bathtub and give them a shower with cold water while brushing with an old dirty dish cleaning brush.  Just a rinse basically. 

It probably helps that I always use a liner.


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InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
 User Gallery
Folding@home Statistics
Registered: 04/30/13
Posts: 61,889
Loc: Milky way
Trusted Cultivator
Re: Cultivation General Discussion [Re: Thedillestpickle]
    #25834078 - 02/24/19 07:48 AM (4 years, 10 months ago)

I do the same without a liner. I use a long handled soft bristle brush and some dish soap if it really needs it.


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Offlinemushroomnate
Pstranger
 User Gallery


Registered: 05/17/17
Posts: 3,100
Last seen: 2 days, 13 hours
Re: Cultivation General Discussion [Re: bodhisatta]
    #25834101 - 02/24/19 08:04 AM (4 years, 10 months ago)

Quote:

bodhisatta said:
I do the same without a liner. I use a long handled soft bristle brush and some dish soap if it really needs it.



:whathesaid:
Soap and water.


--------------------


BRF to bulk/Cakes as spawn


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OfflineFailboat
Fuck Up

Registered: 02/01/18
Posts: 8,736
Last seen: 12 hours, 38 minutes
Trusted Cultivator
Re: Cultivation General Discussion [Re: mushroomnate]
    #25834146 - 02/24/19 08:42 AM (4 years, 10 months ago)

I do it in the sink with a classic green scrubby. Got the sprayer to rinse.


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InvisibleYesum
Furry as Fuc
Male User Gallery


Registered: 11/05/12
Posts: 13,124
Loc: Central Part of Town
Re: Cultivation General Discussion [Re: Failboat]
    #25834180 - 02/24/19 08:55 AM (4 years, 10 months ago)

Tub/jar brush.
Heavily used


--------------------


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Invisiblejuniperus
Frim Fram Sauce


Registered: 12/02/18
Posts: 718
Re: Cultivation General Discussion [Re: mushroomnate]
    #25834888 - 02/24/19 02:27 PM (4 years, 10 months ago)

Quote:

mushroomnate said:
Quote:

bodhisatta said:
I do the same without a liner. I use a long handled soft bristle brush and some dish soap if it really needs it.



:whathesaid:
Soap and water.




Same. Wouldn't a good scrubbing with soap and water leave your tub closer to aseptic than using iso? For the same reason surgeons use soap (albeit impregnated with antimicrobial agents), and don't just dunk their arms in alcohol.


--------------------
To avoid all evil, to cultivate good, and to cleanse one's mind - this is the teaching of the Buddhas


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Offlinetryptkaloids
Learner
I'm a teapot


Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
Last seen: 3 days, 14 hours
Trusted Cultivator
Re: Cultivation General Discussion [Re: juniperus]
    #25834940 - 02/24/19 02:54 PM (4 years, 10 months ago)

Yes, soap and water removes everything instead of killing most things


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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OfflinePsilosopherr
A psilly goose
Other User Gallery


Registered: 02/15/12
Posts: 12,278
Last seen: 1 month, 10 days
Re: Cultivation General Discussion [Re: tryptkaloids] * 3
    #25835290 - 02/24/19 05:46 PM (4 years, 10 months ago)

pour lil gasoline in there and light her up. quicker and cleaner than your pussy soap :machinegun:

ha! made you click the thread just to read a dumb joke. Chumps


Edited by Psilosopherr (02/24/19 05:46 PM)


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InvisibleJ. Jack Flash
stranger than ever.

Registered: 11/20/13
Posts: 1,500
Re: Cultivation General Discussion [Re: Psilosopherr] * 1
    #25835486 - 02/24/19 08:09 PM (4 years, 10 months ago)

Quote:

Psilosopherr said:
pour lil gasoline in there and light her up. quicker and cleaner than your pussy soap :machinegun:

ha! made you click the thread just to read a dumb joke. Chumps




what a shitbag.  i approve.

:ruggedwink:


--------------------
 
the j stands for jesus.
2020 new years grow along


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Invisiblesh4d0ws
LSx
I'm a teapot User Gallery

Registered: 02/26/08
Posts: 12,086
Re: Cultivation General Discussion [Re: J. Jack Flash]
    #25838823 - 02/26/19 10:51 AM (4 years, 10 months ago)

So I'm currently experimenting with casing layers at various times through spawn (full colonization, after knotting, after small blobs appear, 80% colonization) and so far I'm still getting blob city.

The only time I've gotten this PE variety to put mostly normal fruits from flush 1 is on PF cakes. Does anyone have experience top fruiting grain jars, or using straight grain spawn in shoeboxes, or something similar? I'm tired of blobs and if I can't get a casing layer to work I'm wondering about alternative fruiting methods.

Also thinking about trying to g2g some of my jars to bags and then just fruit the bags of grain. Maybe it won't blob.


Damn blobs!


--------------------


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OfflineShaperDreaming
Weirdo
Female User Gallery


Registered: 10/30/18
Posts: 3,429
Loc: United States Flag
Last seen: 2 years, 3 days
Re: Cultivation General Discussion [Re: sh4d0ws]
    #25838837 - 02/26/19 10:58 AM (4 years, 10 months ago)

Hey sh4d0ws, I'm doing the same. I just gave a super thick top-layer of coir for a recent PE spawn seeing if that helps, as currently I am just getting blob city for flush one, then half blobs flush two. I like the variety enough to master it... so this will take a while I'm sure. 

Another thing I'm thinking about doing is keeping the grains on the bottom of a shoebox, then just adding coir on top. I'm guessing that the technique for non-blobbing will involve coaxing the mushroom to fruit "proper" rather than "lazy". (Look, I don't know enough about this hobby to talk more scientifically, just think like a plant with me for a second).

My hope is that from bottom fruiting? bottom colonizing? bottom spawning?... putting all the coir over the colonized grain rather than mixed in will be that the PE has to work so hard to get to a place where it can fruit (at the top) that it will try harder to make spore producers rather than lazy af blobs.

I've also considered doing grains bottom, coir middle, then cactus soil top to see if that gives it even more reasons...

Curious to see how your adventures continue.


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InvisibleCaps McGee
Grandaddy Smurfshack
I'm a teapot User Gallery


Registered: 10/28/17
Posts: 14,357
Loc: ally known as ... Flag
Re: Cultivation General Discussion [Re: sh4d0ws]
    #25838840 - 02/26/19 10:59 AM (4 years, 10 months ago)

Try cutting the spawn ratio to 1:2 or less... IME, some varieties/ cultures are sensitive to high nutrition substrates it seems... for this reason,  I don't see cased grains fixing it, but rather making it worse... I'd try 1:2 with coir only, and a top layer of coir only at spawn

Blobs are active AF too ya know?


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Invisiblesh4d0ws
LSx
I'm a teapot User Gallery

Registered: 02/26/08
Posts: 12,086
Re: Cultivation General Discussion [Re: Caps McGee]
    #25838882 - 02/26/19 11:20 AM (4 years, 10 months ago)

I've tried 1:2 and still blob city lol. I wanna get my hands on the blobless PE. And yeah they are active and supposed to be very potent I haven't tried them yet. I have a ton of APE "blueberries" some of my shoeboxes had 50-100 of these blobs. I dunno about their potency hopefully they are strong like APE  :acidcat:


--------------------


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OfflineShaperDreaming
Weirdo
Female User Gallery


Registered: 10/30/18
Posts: 3,429
Loc: United States Flag
Last seen: 2 years, 3 days
Re: Cultivation General Discussion [Re: Caps McGee]
    #25838883 - 02/26/19 11:21 AM (4 years, 10 months ago)

Quote:

Caps McGee said:
Try cutting the spawn ratio to 1:2 or less... IME, some varieties/ cultures are sensitive to high nutrition substrates it seems... for this reason,  I don't see cased grains fixing it, but rather making it worse... I'd try 1:2 with coir only, and a top layer of coir only at spawn



Right now I'm at 1qt grain, 2qt coir to mix, 1qt coir top layer (last attempt to not get blobs), so closer to 1:3

Quote:

Caps McGee said:
Blobs are active AF too ya know?



Oh, I ain't throwing these fuckers out :laugh2:

I just want more of them... in dick form.

Quote:

sh4d0ws said:
And yeah they are active and supposed to be very potent I haven't tried them yet.



:sammy::llamastare:
What?! EAT THEM OMG


Edited by ShaperDreaming (02/26/19 11:23 AM)


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Invisiblestareatclouds
star eat clouds?
 Unread Journal User Gallery


Registered: 09/29/14
Posts: 9,887
Trusted Cultivator
Re: Cultivation General Discussion [Re: Caps McGee]
    #25839077 - 02/26/19 01:20 PM (4 years, 10 months ago)

Quote:

Caps McGee said:
Try cutting the spawn ratio to 1:2 or less... IME, some varieties/ cultures are sensitive to high nutrition substrates it seems... for this reason,  I don't see cased grains fixing it, but rather making it worse... I'd try 1:2 with coir only, and a top layer of coir only at spawn




Do you have any documented grows showing this sensitivity in subs with higher nutrients?


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