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Offlinegizmodo
Woodland Creature
Male


Folding@home Statistics
Registered: 06/21/18
Posts: 2,064
Loc: Behind You
Last seen: 2 years, 8 months
Re: Cultivation General Discussion [Re: bodhisatta]
    #25831157 - 02/22/19 11:07 PM (4 years, 11 months ago)

Quote:

bodhisatta said:
Quote:

LtLurker said:
Tormato threw this pic up on another page.


Anyone else wear a respirator for sterile work? Way more comfortable than a paper mask. I like to think it diffuses the air disturbance from breathing/talking better as well.



You don't need a mask at all. I talked in all my videos without a mask at all. If your breathing is effecting your work something else needs to be fixed about your procedure. Like not having your mouth above sterile work




We need another mushboy drawn diagram of mouth drippings.


--------------------

One must be mindful to maintain their balance on the slippery earth.

Bod's Comprehensive Agar Resource
Gizmodo's Market Stall
Say No To Grow Kits


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InvisibleCaps McGee
Grandaddy Smurfshack
I'm a teapot User Gallery


Registered: 10/28/17
Posts: 14,357
Loc: ally known as ... Flag
Re: Cultivation General Discussion [Re: gizmodo]
    #25831278 - 02/23/19 01:09 AM (4 years, 11 months ago)

So how many cycles are you guys getting away with, with poly stuffed lids, without changing the polyfil?


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πŸ„πŸ’šπŸ˜Ž

Ask Caps! (My Q&A Thread)
Caps' Comprehensive Cultivation Tek List


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OfflineDoc9151M
Mycologist
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Registered: 02/23/17
Posts: 13,753
Loc: Gulf Coast USA Flag
Last seen: 1 year, 6 months
Trusted Cultivator
Re: Cultivation General Discussion [Re: Yesum]
    #25831304 - 02/23/19 01:50 AM (4 years, 11 months ago)

Quote:

Yesum said:
Quote:

tryptkaloids said:
Quote:

icetech said:
Jesus Yesum.. what are you using for agar? my myc never looks that thick or perfect..

Quote:

Yesum said:
Nice. Those are healthy looking cakes.

I'm gona do a little agar pic dump.

150mm plates.


And this fella is maz.  It's a fruit from one of my plates and it appears to be sporeless. :woot:






i cant shake the feeling something else is coming along for the ride




Which one are you thinking.



I like the sector in the 1st picture that covers the eleven to twelve o'clock position. Did you take anything from that area?


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Psilocybe cubensis data collection thread. please help with this project if you hunt wild cubensis.
https://www.shroomery.org/forums/showflat.php?Cat=0&Number=26513593&page=0&vc=1#26513593


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InvisibleYesum
Furry as Fuc
Male User Gallery


Registered: 11/05/12
Posts: 13,124
Loc: Central Part of Town
Re: Cultivation General Discussion [Re: Doc9151]
    #25831426 - 02/23/19 05:06 AM (4 years, 11 months ago)

You know I did. Lol. Fo shizzle


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Offlineicetech
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Registered: 08/21/17
Posts: 3,450
Loc: FSM's loving noodles.
Last seen: 3 months, 5 days
Re: Cultivation General Discussion [Re: Yesum]
    #25831458 - 02/23/19 06:01 AM (4 years, 11 months ago)

I haven't read up a ton on using LC, what is it about it that makes you stick with it? I have seen it colonized crazy fast, is it much harder to make than just doing agar?

Quote:

Thedillestpickle said:
Quote:

stareatclouds said:
What size of Whatman do you have on there? No other modifications on the lid, such as SHIP or anything? Plastic or metal?




I really think I should write a tek on this because I've dialed it in a lot over the years. I saw the advantages of LC early on and never looked back, and have got it to the point where contamination isn't a concern so much anymore.  I've gone through many iterations for LC jar lids.  I use a larger size Whatman filter, not sure if it's critical to use a larger filter, but I like to think it helps.  They are 50mm 0.3 micron filters.  In retrospect it's probably not the filter letting contams in as much as a leak somewhere in the lid. Either way, tinfoil has helped with that. I had a hell of a time drilling holes in the lids and some of the holes got a little bigger than I planned.  I use plastic lids from amazon.  They seem to seal up pretty good with a strong twist.  One trick I finally figured out on the last lid was to use a dremel tool to get the hole to the desired size rather than attempting to drill with a drill bit which resulted in many cracked lids.

Because I am using larger filters I was also able to slip a small piece of silicone tube over the inside vent on the filter and I stuff a small wad of polyfil into that tube.  This modification helps prevent slashing LC from entering the whatman filter and soaking the SFD inside. 

I use SHIPs, and I don't replace them often at all but I always try to line the syringe up directly with the already existing hole, so that I'm not piercing the same piece of rubber in a dozen places.  I don't trust them as a vector so I usually spray them with alcohol right before filling syringes.  Keeping tinfoil over the top has been a great help.

For syringes I have a totally different way of reusing them.  I have 60ml syringes with 18 gauge needles.  I simply place them empty into my pressure cooker and get them to 15psi for 30 minutes.  They have been used dozens of times and are all filthy but I don't care. 

I use 1 gallon jars.  I add 5 grams agar to every 2 liters LC.  This gels it up slightly, allowing it to be shaken into a slurry that suspends bubbles.  It's possible that these bubbles help provide extra oxygen, although I'd have to do some proper chemistry calculations to determine whether it was a significant.  It also changes the way contaminants show up in the LC.  I can always spot contams in the LC without needing to test on agar.  Bacteria contam will give a yellow hue to the surface, and molds will grow out and even sporulate visibly within the LC.  Healthy mycelium will colonize it in a very distinct way and you can tell when it's ready when you see the telltale ice crystal looking growths all throughout the gel stuck to the walls of the jar.  Contaminated jars either won't develop those "ice crystals" or will show some obvious sign of contamination. 

Unlike typical LC, you don't get isolated clumps of growth filling only 1/3 of the jar of LC.  Instead you get completely homogenized distribution of suspended mycelium in each syringe.  Colonization occurs uniformly throughout the bag or jar and initiates on every grain all at the same time.

Main reasons why I will never stop using my LC technique:
-less work than prepping a hundred plates of agar on a regular basis
-I can make enough innoculant that I never need to grain to grain transfer




--------------------


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Offlineicetech
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Registered: 08/21/17
Posts: 3,450
Loc: FSM's loving noodles.
Last seen: 3 months, 5 days
Re: Cultivation General Discussion [Re: icetech]
    #25831461 - 02/23/19 06:02 AM (4 years, 11 months ago)

It's weird but one of my favorite things in this hobby for me is when you transfer agar>agar and the next day you see those little white fingers all stretching out to get at that new food.. YES MY BABIES!!!! LIVE!!!!


--------------------


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InvisibleBumholio
What's the craic
 User Gallery


Registered: 07/23/18
Posts: 4,269
Loc: Shroomsville
Re: Cultivation General Discussion [Re: Caps McGee]
    #25831464 - 02/23/19 06:03 AM (4 years, 11 months ago)

Quote:

Caps McGee said:
So how many cycles are you guys getting away with, with poly stuffed lids, without changing the polyfil?



I dont change it unless its loose and about to fall out:shrug:


--------------------


"great things may come to those who wait, but only what's left by those who hustle"


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Offlineteladi
FUNKSOULBROTHER

Registered: 06/27/17
Posts: 1,189
Loc: South Africa Flag
Last seen: 5 months, 22 days
Re: Cultivation General Discussion [Re: Caps McGee]
    #25831535 - 02/23/19 06:49 AM (4 years, 11 months ago)

Quote:

Caps McGee said:
So how many cycles are you guys getting away with, with poly stuffed lids, without changing the polyfil?




I change my LC polyfill every 4 or 5 uses. No reason beyond caution. I do like Bumholio for grain jars, and only replace when its fallen out, or looking mank.


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InvisibleBoogieman47
Let's boogie
Male User Gallery

Registered: 03/05/16
Posts: 9,712
Loc: Under your bed Flag
Re: Cultivation General Discussion [Re: teladi]
    #25831593 - 02/23/19 07:35 AM (4 years, 11 months ago)

I ran poly in my lids for about a year before the lids rusted out ... so I'd say at least 500-600 cycles


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InvisibleCaps McGee
Grandaddy Smurfshack
I'm a teapot User Gallery


Registered: 10/28/17
Posts: 14,357
Loc: ally known as ... Flag
Re: Cultivation General Discussion [Re: Boogieman47]
    #25831645 - 02/23/19 08:08 AM (4 years, 11 months ago)

Holy shit... I've swapped them out everytime!
:kittylaugh:
Didn't even occur to reuse them until last night (this morning really:5 hours ago (gotta love mania)) when hunting down 10 qts to load oats into this morning... I was like "I mean, still looks good!" Lol


--------------------
πŸ„πŸ’šπŸ˜Ž

Ask Caps! (My Q&A Thread)
Caps' Comprehensive Cultivation Tek List


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Offlineicetech
 User Gallery


Registered: 08/21/17
Posts: 3,450
Loc: FSM's loving noodles.
Last seen: 3 months, 5 days
Re: Cultivation General Discussion [Re: Caps McGee]
    #25831667 - 02/23/19 08:22 AM (4 years, 11 months ago)

Quote:

Caps McGee said:
Holy shit... I've swapped them out everytime!
:kittylaugh:
Didn't even occur to reuse them until last night (this morning really:5 hours ago (gotta love mania)) when hunting down 10 qts to load oats into this morning... I was like "I mean, still looks good!" Lol




Yeah i was gonna swap mine before the next batch then was like WTF.. going in the PC anyway..


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OfflineThedillestpickle
cultured
Male


Registered: 02/02/16
Posts: 1,170
Loc: Canada Flag
Last seen: 3 years, 7 months
Re: Cultivation General Discussion [Re: icetech]
    #25831737 - 02/23/19 08:54 AM (4 years, 11 months ago)

@ icetech

I get enough LC to inoculate 24 bags from a single jar of LC.  I can prep 8 jars of LC at once with about the same amount of effort as prepping a sleeve of dishes.  So for an equal amount of work I'm getting enough to inoculate 192 bags. For me that works out to 128 large monotubs.

So yea it's made preparing spawn a lot easier and faster. 
The appeal is any other method would be a lot more work for me.


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InvisibleMadHatter333
We Are All Mad Here

Registered: 09/20/17
Posts: 4,650
Loc: Your Mom’s Rabbit Hole
Re: Cultivation General Discussion [Re: Thedillestpickle]
    #25831776 - 02/23/19 09:17 AM (4 years, 11 months ago)

Hey guys and gals.

Had a question about popping lids. I had a shoebox that had pins 1/4 up to the lid. I popped one side for FAE and it seemed the side I popped had mushrooms mature faster than the other side that wasn’t popped.

Is popping one side of the lid not recommended? I got six APE shoeboxes I popped one side on because the surface was getting to moist. Just curious if I should keep both sides popped. What’s people’s take on popping lids?


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Offlinetryptkaloids
Learner
I'm a teapot


Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
Last seen: 3 days, 12 hours
Trusted Cultivator
Re: Cultivation General Discussion [Re: MadHatter333]
    #25831787 - 02/23/19 09:25 AM (4 years, 11 months ago)

If its for fae do as neccesary, if it's for space I unclip both sides and let the mushrooms stretch as they will


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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Offlinemushroomnate
Pstranger
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Registered: 05/17/17
Posts: 3,100
Last seen: 2 days, 11 hours
Re: Cultivation General Discussion [Re: MadHatter333]
    #25831789 - 02/23/19 09:26 AM (4 years, 11 months ago)

I like to pop the lids/dub right at the end.
Seem like whenever I dubtub or pop the lids,24 hrs later they're done.
For me it's like the FAE finisher.
With some of my APE tho,I never even needed to pop the lids.:shrug:


--------------------


BRF to bulk/Cakes as spawn


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Offlineicetech
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Registered: 08/21/17
Posts: 3,450
Loc: FSM's loving noodles.
Last seen: 3 months, 5 days
Re: Cultivation General Discussion [Re: Thedillestpickle]
    #25831812 - 02/23/19 09:40 AM (4 years, 11 months ago)

Jesus.. i'm gonna read up on doing that... i don't mind agar at all. but it just takes soo long for everything to grow out and i only do like 2 grows a year and start way later than i should.

Quote:

Thedillestpickle said:
@ icetech

I get enough LC to inoculate 24 bags from a single jar of LC.  I can prep 8 jars of LC at once with about the same amount of effort as prepping a sleeve of dishes.  So for an equal amount of work I'm getting enough to inoculate 192 bags. For me that works out to 128 large monotubs.

So yea it's made preparing spawn a lot easier and faster. 
The appeal is any other method would be a lot more work for me.




--------------------


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InvisibleMadHatter333
We Are All Mad Here

Registered: 09/20/17
Posts: 4,650
Loc: Your Mom’s Rabbit Hole
Re: Cultivation General Discussion [Re: mushroomnate]
    #25831841 - 02/23/19 09:53 AM (4 years, 11 months ago)

Thanks guys. I may need to clip the lids back down on some of the APE boxes so they don’t loose to much moisture. The surface was looking a bit to moist for my liking though. May have seen a baby pin or two in one of the boxes. Got some decent knots going. They’re taking forever though!!!


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InvisibleCaps McGee
Grandaddy Smurfshack
I'm a teapot User Gallery


Registered: 10/28/17
Posts: 14,357
Loc: ally known as ... Flag
Re: Cultivation General Discussion [Re: MadHatter333]
    #25832703 - 02/23/19 04:03 PM (4 years, 11 months ago)

Yeah, I've been leaving them snapped until they reach the lid and need dubtubbed... gets plenty of FAE and maintains humidity much more efficiently... not peeking a lot seems to help too


--------------------
πŸ„πŸ’šπŸ˜Ž

Ask Caps! (My Q&A Thread)
Caps' Comprehensive Cultivation Tek List


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Invisiblestareatclouds
star eat clouds?
 Unread Journal User Gallery


Registered: 09/29/14
Posts: 9,887
Trusted Cultivator
Re: Cultivation General Discussion [Re: Thedillestpickle]
    #25832706 - 02/23/19 04:05 PM (4 years, 11 months ago)

Quote:

Thedillestpickle said:
Quote:

stareatclouds said:
What size of Whatman do you have on there? No other modifications on the lid, such as SHIP or anything? Plastic or metal?




I really think I should write a tek on this because I've dialed it in a lot over the years. I saw the advantages of LC early on and never looked back, and have got it to the point where contamination isn't a concern so much anymore.  I've gone through many iterations for LC jar lids.  I use a larger size Whatman filter, not sure if it's critical to use a larger filter, but I like to think it helps.  They are 50mm 0.3 micron filters.  In retrospect it's probably not the filter letting contams in as much as a leak somewhere in the lid. Either way, tinfoil has helped with that. I had a hell of a time drilling holes in the lids and some of the holes got a little bigger than I planned.  I use plastic lids from amazon.  They seem to seal up pretty good with a strong twist.  One trick I finally figured out on the last lid was to use a dremel tool to get the hole to the desired size rather than attempting to drill with a drill bit which resulted in many cracked lids.

Because I am using larger filters I was also able to slip a small piece of silicone tube over the inside vent on the filter and I stuff a small wad of polyfil into that tube.  This modification helps prevent slashing LC from entering the whatman filter and soaking the SFD inside. 

I use SHIPs, and I don't replace them often at all but I always try to line the syringe up directly with the already existing hole, so that I'm not piercing the same piece of rubber in a dozen places.  I don't trust them as a vector so I usually spray them with alcohol right before filling syringes.  Keeping tinfoil over the top has been a great help.

For syringes I have a totally different way of reusing them.  I have 60ml syringes with 18 gauge needles.  I simply place them empty into my pressure cooker and get them to 15psi for 30 minutes.  They have been used dozens of times and are all filthy but I don't care. 

I use 1 gallon jars.  I add 5 grams agar to every 2 liters LC.  This gels it up slightly, allowing it to be shaken into a slurry that suspends bubbles.  It's possible that these bubbles help provide extra oxygen, although I'd have to do some proper chemistry calculations to determine whether it was a significant.  It also changes the way contaminants show up in the LC.  I can always spot contams in the LC without needing to test on agar.  Bacteria contam will give a yellow hue to the surface, and molds will grow out and even sporulate visibly within the LC.  Healthy mycelium will colonize it in a very distinct way and you can tell when it's ready when you see the telltale ice crystal looking growths all throughout the gel stuck to the walls of the jar.  Contaminated jars either won't develop those "ice crystals" or will show some obvious sign of contamination. 

Unlike typical LC, you don't get isolated clumps of growth filling only 1/3 of the jar of LC.  Instead you get completely homogenized distribution of suspended mycelium in each syringe.  Colonization occurs uniformly throughout the bag or jar and initiates on every grain all at the same time.

Main reasons why I will never stop using my LC technique:
-less work than prepping a hundred plates of agar on a regular basis
-I can make enough innoculant that I never need to grain to grain transfer




Thanks for the reply. I'm not super well versed on LCs and just bought my Whatman's, but a few thoughts/questions:

- I agree on the filter not being the issue. That's why I asked initially.

- Plastic lids suck for liquids IMO as I've had them warp and not fit on properly before. Have you tried this with regular metal lids?

- Again, I haven't even used my Whatman's yet, but why do you have an SFD and other things with it? I was under the impression that the micron rating is so fine that you shake the jar quite a bit without leakage.

- Regarding plastic lids cracking, a Forstner bit is my preferred choice. Perfect circles in both metal and plastic. One trick to prevent cracked lids is to cut out cardboard in circles to fit inside the lid. Put enough of them together so when you fit them snugly inside the lid threading, it portudes. Then put the lid on something solid, face up, and push down hard while drilling slowly.

- "For syringes I have a totally different way of reusing them..." You just mean PCing them again? I also do that and thought it was standard.

- The more agar heavy mixture that allows bacteria to form is fascinating, though. Do you have any pictures of this?

- How much LC do you use per bag? And how are you inoculating the LC initially?

- Regarding foil helping protect SHIP and Whatman and such: I put together some little contraptions a while back I haven't tested yet. I cut a hole into a plastic lid and RTV'd the blue cap to one of my slants over it, upside down, with RTV in the middle. And then I screwed the slant onto it. So I can unscrew it and there's a sterile SHIP there for me.


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Offlineicetech
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Registered: 08/21/17
Posts: 3,450
Loc: FSM's loving noodles.
Last seen: 3 months, 5 days
Re: Cultivation General Discussion *DELETED* [Re: stareatclouds]
    #25832776 - 02/23/19 04:31 PM (4 years, 11 months ago)

Post deleted by icetech

Reason for deletion: didn't post right


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