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InvisibleShroomawayer
I'm a teapot


Registered: 08/24/16
Posts: 3
Re: Cultivation General Discussion [Re: Caps McGee]
    #25806846 - 02/11/19 10:44 PM (4 years, 11 months ago)

I've been noticing some slow growth on my jars recently. Rye grain prep hasn't changed and I've been using the same clones/isolate cultures as previous flushes.


Read a bit about slowed growth when going A2G potentially being caused by the mycelium having to "switch gears." From sugar media to the starch in grains. Anyone run into a similiar issue? Potentially solved by doing a GLC with the soak water. Which I'm PC'ing now. Will get back with updates to see if that sorts it out.



Curious to see if the GLC will colonize faster if nocc'd with a colonized grain or too or from an agar wedge...🤔


--------------------
"If you do not change direction, you may end up where you are heading" -Lao Tzu


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InvisibleBph
Stranger
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Registered: 10/11/18
Posts: 1,466
Re: Cultivation General Discussion [Re: SunnyDayze]
    #25806862 - 02/11/19 11:01 PM (4 years, 11 months ago)

Quote:

SunnyDayze said:
Yes, so the bag isn’t rubbing the roof and I don’t have to shove the jar in the bag to dump.  I turned the bricks up on their edge and I’m going to give it a go unless someone else has an idea (using materials I have on hand).



Do you have another tote the same size? Stack them open side to open side?


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InvisibleJohnny Dont
500 Don'ts of Knife Safety


Registered: 04/24/14
Posts: 1,830
Re: Cultivation General Discussion [Re: SunnyDayze]
    #25807232 - 02/12/19 06:17 AM (4 years, 11 months ago)

Quote:

SunnyDayze said:

I'm going to be using myco bags for the first time tomorrow morning.  I'm letting my grains dry overnight.  Using large .5 micron filtre bags.
I do not have a fancy flow hood like some people.....so have to work in a SAB.  Also, I am inoculating from a grain jar. 
Any tips?





I dub tub my SAB, a box on a box. The extra room allowed me to open the bags inside without hitting the top. Then I had my donor jar and zip tie stacked on another jar so I wouldn't have to lift them so far up. Tryn to minimize movement.

My first bags came out bacterial, picture for reference



But it could of been my spawn, Idk, I'm still tryn to perfect my tek.

My last round of bags are looking better, no wet spots, but some condensation in parts of the bag.


--------------------
I have left life and loves behind me, to be blown about as the sea desires, to have the freedom of the open air, and to be witness to the making of the world.


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InvisibleSunnyDayze
Finder of shrubberies
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Registered: 12/30/17
Posts: 2,230
Loc: Canada Flag
Re: Cultivation General Discussion [Re: Johnny Dont] * 3
    #25807286 - 02/12/19 06:54 AM (4 years, 11 months ago)

Bph and Johnny, I looked around for another tub to use but can’t find one.  My SAB is bigger than any other tote I have, thought about cutting a lid to put a smaller tote on my SAB.  I live an hour away from where I can get another tub.  Thanks for the idea though!  I was wondering if you would put some type of platform in there for your jars.

I am ordering a hepa filtre today, going to the states next week and I can pick it up down there.  Had enough fun with the SAB but it’s time to trade up lol I haven’t told it yet, have to practice my whole “it’s not you, it’s me “ line.


--------------------


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InvisibleJ. Jack Flash
stranger than ever.

Registered: 11/20/13
Posts: 1,500
Re: Cultivation General Discussion [Re: SunnyDayze]
    #25807311 - 02/12/19 07:12 AM (4 years, 11 months ago)

:highfive: everyone's getting a flow hood!

meanwhile, elevate the sab on the table to whatever height you need using anything stable. phone books at the corners or something. then wrap the gap between the box and the table with the plastic sheet. tape it or clip it to the lip. only needs to block drafts, right? forget where i saw it, but there's pictures of a sab literally made of trash. crumpled cardboard and plastic wrapped taped together. looked janky a fuck but reportedly worked fine.


--------------------
 
the j stands for jesus.
2020 new years grow along


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InvisibleJohnny Dont
500 Don'ts of Knife Safety


Registered: 04/24/14
Posts: 1,830
Re: Cultivation General Discussion [Re: J. Jack Flash]
    #25807335 - 02/12/19 07:29 AM (4 years, 11 months ago)

I just stacked the jars on other jars, would of used a platform if I had one. And my two boxes were not the same size, so I taped them together were they didn't over lap right


--------------------
I have left life and loves behind me, to be blown about as the sea desires, to have the freedom of the open air, and to be witness to the making of the world.


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Offlinetryptkaloids
Learner
I'm a teapot


Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
Last seen: 3 days, 12 hours
Trusted Cultivator
Re: Cultivation General Discussion [Re: Psilosopherr]
    #25807546 - 02/12/19 09:46 AM (4 years, 11 months ago)

Quote:

Psilosopherr said:
i was under the impression that agar was the best way to test sterile technique but I must be taking something out of context there.

Chinese methods are weird. This guy is knocking enriched sawdust in open air on a dirt floor. I also hear some of them pasteurize with steam piped under big tarps covering huge piles of bags with no spaces between them. Its all rather interesting




agar is the best way to clean and expand cultures... My main point is opening and handling a jar lid is different than taking a transfer. Therefore easier for holes in your technique. You use different muscles to hold a jar than you do a plate, plates take more dexterity and jars take more strength. Just more room for something to go wrong ime.  That seem like a sketch way to do bags.


Quote:

MushroomBush said:
Bacteria can hide on agar, bad grain prep and technique are the main source of bacteria


this is frustrating not knowing for sure where its coming from


you'll get it figured out. Just keep at it. Post pics every step of the way and videos of your a2g. We may be able to spot holes you dont.  Are you using mad season's Hidden contamination guide?
Quote:

Caps McGee said:
No gasket for one piece plastic lids no


you should quote who you're replying to...


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


Edited by tryptkaloids (02/12/19 09:59 AM)


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OfflineDayglo Joe


Registered: 08/15/18
Posts: 101
Last seen: 2 years, 3 months
Re: Cultivation General Discussion [Re: tryptkaloids]
    #25807705 - 02/12/19 11:09 AM (4 years, 11 months ago)

Quote:

tryptkaloids said:
Quote:

Psilosopherr said:

i was under the impression that agar was the best way to test sterile technique but I must be taking something out of context there.

Chinese methods are weird. This guy is knocking enriched sawdust in open air on a dirt floor. I also hear some of them pasteurize with steam piped under big tarps covering huge piles of bags with no spaces between them. Its all rather interesting




agar is the best way to clean and expand cultures... My main point is opening and handling a jar lid is different than taking a transfer. Therefore easier for holes in your technique. You use different muscles to hold a jar than you do a plate, plates take more dexterity and jars take more strength. Just more room for something to go wrong ime.  That seem like a sketch way to do bags.


Quote:

MushroomBush said:
Bacteria can hide on agar, bad grain prep and technique are the main source of bacteria


this is frustrating not knowing for sure where its coming from


you'll get it figured out. Just keep at it. Post pics every step of the way and videos of your a2g. We may be able to spot holes you dont.  Are you using mad season's Hidden contamination guide?
Quote:

Caps McGee said:
No gasket for one piece plastic lids no


you should quote who you're replying to...





Interesting discussion
Thanks for the clarification
As I was thinking the same as Psilosopherr.


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OfflineDayglo Joe


Registered: 08/15/18
Posts: 101
Last seen: 2 years, 3 months
Re: Cultivation General Discussion [Re: tryptkaloids]
    #25807722 - 02/12/19 11:15 AM (4 years, 11 months ago)

Quote:

tryptkaloids said:
Quote:

Psilosopherr said:
i was under the impression that agar was the best way to test sterile technique but I must be taking something out of context there.




agar is the best way to clean and expand cultures... My main point is opening and handling a jar lid is different than taking a transfer. Therefore easier for holes in your technique. You use different muscles to hold a jar than you do a plate, plates take more dexterity and jars take more strength. Just more room for something to go wrong ime. 






:takingnotes:


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OfflinePsilosopherr
A psilly goose
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Registered: 02/15/12
Posts: 12,278
Last seen: 1 month, 10 days
Re: Cultivation General Discussion [Re: Dayglo Joe]
    #25807809 - 02/12/19 11:53 AM (4 years, 11 months ago)

Pouring grain is harder than working with a scalpel? Different people different problems I guess.

came across this soak only grain prep tek and like the idea of it, so I'm sharing it here in case anyone else likes the idea of it. Sounds like it doesn't matter if the outside of the grain is dry or not with this method, I'm all about that


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OfflineFailboat
Fuck Up

Registered: 02/01/18
Posts: 8,736
Last seen: 11 hours, 29 minutes
Trusted Cultivator
Re: Cultivation General Discussion [Re: Psilosopherr]
    #25807854 - 02/12/19 12:23 PM (4 years, 11 months ago)

I feel like if one were so inclined to build an extra large SAB so as to accommodate bag work it would be pretty cheap and I doubt it much less effective than fumbling around in a small tote mod. Just a thought.


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InvisibleSunnyDayze
Finder of shrubberies
Female User Gallery


Registered: 12/30/17
Posts: 2,230
Loc: Canada Flag
Re: Cultivation General Discussion [Re: Failboat] * 3
    #25807885 - 02/12/19 12:37 PM (4 years, 11 months ago)

Much more effective, I agree Quirk.  Someone should build one and do a tek but it won’t be me because.......

I just ordered my new Hepa filtre!!!

:fuckyeahdance:

Lol I probably shouldn’t brag yet but I’m a bit excited.


--------------------


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Offlinetryptkaloids
Learner
I'm a teapot


Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
Last seen: 3 days, 12 hours
Trusted Cultivator
Re: Cultivation General Discussion [Re: Psilosopherr]
    #25807907 - 02/12/19 12:50 PM (4 years, 11 months ago)

Quote:

Psilosopherr said:
Pouring grain is harder than working with a scalpel? Different people different problems I guess.




:justno: I didnt say that. I said there's more room for error and you can absolutely get bacteria from bad procedure, and if the bad procedure is during the grain inoculation then agar wont tell you


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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OfflineFerather
Mycological
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Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom Flag
Last seen: 1 year, 2 months
Re: Cultivation General Discussion [Re: SunnyDayze]
    #25807908 - 02/12/19 12:50 PM (4 years, 11 months ago)

https://www.shroomery.org/forums/showflat.php/Number/25794328#25794328

https://www.shroomery.org/forums/showflat.php/Number/25792298#25792298

----

As soon as I opened the container (sudden increase in oxygen) some aerial mycelium bruised, about 4 seconds after mixing several areas bruised.
Smell, 50-50 fungi-honey (glucose in various formats, from starch), I apparently have hyper glucose production (more than normal).

This most likely is due to T-Gel, where phenols (not made from glucose) must be converted into glucose.
I have spawned two containers, both are the same amount, both are WL-Tek as the media.


           


--------------------
                   

Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

DTS DCH Driver for Realtek [DTS:X] - Unlocked and reprogrammed.


Edited by Ferather (02/12/19 01:22 PM)


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InvisibleCaps McGee
Grandaddy Smurfshack
I'm a teapot User Gallery


Registered: 10/28/17
Posts: 14,357
Loc: ally known as ... Flag
Re: Cultivation General Discussion [Re: tryptkaloids]
    #25807948 - 02/12/19 01:07 PM (4 years, 11 months ago)

Quote:

tryptkaloids said:
you should quote who you're replying to...



I figure they'll recognize a direct answer to their question... was a page back and read on before responding... I couldn't tell you who it was now, but they know I'm addressing their question I'm sure... have started consciously trying to make reference to the posts I'm responding to,  this just seemed fairly straight forward :shrug:


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Offlinetryptkaloids
Learner
I'm a teapot


Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
Last seen: 3 days, 12 hours
Trusted Cultivator
Re: Cultivation General Discussion [Re: Caps McGee]
    #25808300 - 02/12/19 03:51 PM (4 years, 11 months ago)

I missed that post so i was confused as fuck :lol: forgive me i hadn't had my morning dab


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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InvisibleSunnyDayze
Finder of shrubberies
Female User Gallery


Registered: 12/30/17
Posts: 2,230
Loc: Canada Flag
Re: Cultivation General Discussion [Re: tryptkaloids]
    #25808466 - 02/12/19 05:06 PM (4 years, 11 months ago)

I had a whole batch of (Ecuador) jars contaminate.  I g2g’d them Jan 16, 10 jars from 2 masters.  Almost ever jar had mushrooms growing in them but every jar also had un colonized grain at the top. Most jars don’t even have much extra moisture.

These little Ecuador’s are fighters man!  :boxer:

I’m thinking it had to be something in the pc process but I do it the same every time.  Vent 20 minutes, 18lbs, 2 hours. 

Any ideas?



--------------------


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OfflineFailboat
Fuck Up

Registered: 02/01/18
Posts: 8,736
Last seen: 11 hours, 29 minutes
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Re: Cultivation General Discussion [Re: SunnyDayze]
    #25808487 - 02/12/19 05:15 PM (4 years, 11 months ago)

Your filters are pooped


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Offlinetryptkaloids
Learner
I'm a teapot


Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
Last seen: 3 days, 12 hours
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Re: Cultivation General Discussion [Re: Failboat] * 1
    #25808504 - 02/12/19 05:23 PM (4 years, 11 months ago)

Quote:

SunnyDayze said:
I had a whole batch of (Ecuador) jars contaminate.  I g2g’d them Jan 16, 10 jars from 2 masters.  Almost ever jar had mushrooms growing in them but every jar also had un colonized grain at the top. Most jars don’t even have much extra moisture.

These little Ecuador’s are fighters man!  :boxer:

I’m thinking it had to be something in the pc process but I do it the same every time.  Vent 20 minutes, 18lbs, 2 hours. 

Any ideas?




those are definately bacterial, but im pretty sure they stalled because they dried out at the top


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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OfflineFailboat
Fuck Up

Registered: 02/01/18
Posts: 8,736
Last seen: 11 hours, 29 minutes
Trusted Cultivator
Re: Cultivation General Discussion [Re: tryptkaloids]
    #25808510 - 02/12/19 05:26 PM (4 years, 11 months ago)

That was my initial thought, i had the same thing happen before.


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