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OfflinecronicrFacebook
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Re: Cultivation General Discussion [Re: Sporepoise]
    #23873494 - 11/27/16 09:15 PM (7 years, 2 months ago)

Toss that


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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InvisibleBoogieman47
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Re: Cultivation General Discussion [Re: Sporepoise]
    #23873495 - 11/27/16 09:15 PM (7 years, 2 months ago)

Bacteria leads to metabolites that tray is definitely no bueno man


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OfflineSporepoise
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Re: Cultivation General Discussion [Re: Boogieman47]
    #23873502 - 11/27/16 09:18 PM (7 years, 2 months ago)

:frown: Will do thanks guys.


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InvisibleMoabfighter
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Re: Cultivation General Discussion [Re: Sporepoise]
    #23873540 - 11/27/16 09:35 PM (7 years, 2 months ago)

I'm now on the path for killer genetics.

I think after I spawn these 18 jars, I'd like to take a step back and work on something fantastic before I make more tubs. LilOldMe has great tubs, and pasty among others say it's the genetics, not how he's running it. Which I agree with.

I can make agar. I can do transfers etc.

Now how do I get killer genes? Slants? Keep transferring a thousand times? What's the method to the madness.


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InvisibleBoogieman47
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Re: Cultivation General Discussion [Re: Moabfighter]
    #23873557 - 11/27/16 09:38 PM (7 years, 2 months ago)

Tons of transferring and testing is the only way to find great genetics once you get a killer tub slant it and either keep on transferring to get better results or just work with that slant ... lil has a bad ass gt culture but you can have killer tubs with giving the best enviorment for your mushies


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OfflinecronicrFacebook
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Re: Cultivation General Discussion [Re: Moabfighter]
    #23873559 - 11/27/16 09:39 PM (7 years, 2 months ago)

Lots of isolates lots of testing


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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Invisibleazur
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Re: Cultivation General Discussion [Re: cronicr]
    #23873571 - 11/27/16 09:43 PM (7 years, 2 months ago)

Isolates, :lolsy:


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A cube is NOT a cube.

FALL IN LOVE WITH LC
FOTTSE!!!
ALL NOOBS READ THIS!!!



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InvisibleMudaFuka
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Re: Cultivation General Discussion [Re: azur]
    #23873582 - 11/27/16 09:46 PM (7 years, 2 months ago)

Cloning is a good short cut. If you start from spores and just isolate. You will have to do tons of test grows some of which might not even be fruiting strains. With a clone at least you have some idea what to expect.


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AMU
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InvisibleJosex
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Re: Cultivation General Discussion [Re: azur]
    #23873590 - 11/27/16 09:49 PM (7 years, 2 months ago)

Oh shit, welcome back! Sup with isolates? I've read you say that you recommend going back to spores several times a year and I'm curious to know the reasoning behind that.
Why would you do that if you have a killer isolate? You can keep a culture for a lot of years without it losing strength, can't you?


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InvisiblePastywhyteMDiscord
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Re: Cultivation General Discussion [Re: Josex]
    #23873594 - 11/27/16 09:51 PM (7 years, 2 months ago)

Quote:

Josex said:
You can keep a culture for a lot of years without it losing strength, can't you?




Yes. It's the basis behind the entire edible industry. Cubes are no different.


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OfflinecronicrFacebook
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Re: Cultivation General Discussion [Re: Josex]
    #23873604 - 11/27/16 09:53 PM (7 years, 2 months ago)

Quote:

Josex said:
Oh shit, welcome back! Sup with isolates? I've read you say that you recommend going back to spores several times a year and I'm curious to know the reasoning behind that.
Why would you do that if you have a killer isolate? You can keep a culture for a lot of years without it losing strength, can't you?



:standingby:


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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Invisibleazur
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Re: Cultivation General Discussion [Re: Pastywhyte]
    #23873609 - 11/27/16 09:57 PM (7 years, 2 months ago)

Quote:

Pastywhyte said:
Quote:

Josex said:
You can keep a culture for a lot of years without it losing strength, can't you?




Yes. It's the basis behind the entire edible industry. Cubes are no different.



I agree....but I also disagree.
Think in nature. Edibles have long lives in nature. Cubes don't. Listen to nature.


--------------------


A cube is NOT a cube.

FALL IN LOVE WITH LC
FOTTSE!!!
ALL NOOBS READ THIS!!!



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InvisibleliloldmeFacebook
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Re: Cultivation General Discussion [Re: MudaFuka]
    #23873611 - 11/27/16 09:58 PM (7 years, 2 months ago)

I took a clone of a lid lifter closed cap from this gt isolate the other day. Curious to see how it turns out :datass:


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InvisibleMudaFuka
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Re: Cultivation General Discussion [Re: liloldme] * 1
    #23873615 - 11/27/16 10:00 PM (7 years, 2 months ago)

A clone of an isolate will be identical to the isolate.


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AMU
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OfflinecronicrFacebook
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Re: Cultivation General Discussion [Re: MudaFuka]
    #23873620 - 11/27/16 10:03 PM (7 years, 2 months ago)

Exactly....just use the isolate.


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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InvisibleMoabfighter
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Re: Cultivation General Discussion [Re: liloldme]
    #23873624 - 11/27/16 10:04 PM (7 years, 2 months ago)

So "once I get a killer tub, slant it"

I keep a chunk of myc on the pasty plate that I inoculated that killer tub with?

That's there I'm not grasping. Keep a thousand pasty plates in the fridge that I'm testing, and the best one, transfer that myc and make a slant??


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KSSS And PE WBS.
 


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InvisiblePastywhyteMDiscord
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Re: Cultivation General Discussion [Re: azur]
    #23873626 - 11/27/16 10:05 PM (7 years, 2 months ago)

Quote:

azur said:
Quote:

Pastywhyte said:
Quote:

Josex said:
You can keep a culture for a lot of years without it losing strength, can't you?




Yes. It's the basis behind the entire edible industry. Cubes are no different.



I agree....but I also disagree.
Think in nature. Edibles have long lives in nature. Cubes don't. Listen to nature.




In nature they don't have refrigerators to put them into periods of slow cell division preventing the shortening of the telomeres. Keep your masters young, rotate their media and they will last a long time.


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InvisibleliloldmeFacebook
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Re: Cultivation General Discussion [Re: Moabfighter]
    #23873633 - 11/27/16 10:08 PM (7 years, 2 months ago)

So you guys are saying you can only expect different results from cloning a fruit from an MS grow? Trying to clone a nice fruitbody from an isolate is pointless?


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InvisibleMudaFuka
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Re: Cultivation General Discussion [Re: liloldme]
    #23873639 - 11/27/16 10:10 PM (7 years, 2 months ago)

Yup. Once you've isolated down to a monoculture. There's only one strain. The genetics can't be narrowed any further.


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Re: Cultivation General Discussion [Re: Moabfighter]
    #23873641 - 11/27/16 10:10 PM (7 years, 2 months ago)

Quote:

Moabfighter said:
So "once I get a killer tub, slant it"

I keep a chunk of myc on the pasty plate that I inoculated that killer tub with?

That's there I'm not grasping. Keep a thousand pasty plates in the fridge that I'm testing, and the best one, transfer that myc and make a slant??




You slant a good isolate or clone. If you have a monoculture that you want to test, transfer from the donor plate to more plates, keep one on standby in the fridge and use the other/s to noc some grains to see how it performs. Same with clones. If it performs well, take the culture from the fridge and slant it.


Edited by Josex (11/27/16 10:17 PM)


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Shop: North Spore Injection Grain Bag   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Left Coast Kratom Buy Kratom Extract   Bridgetown Botanicals CBD Concentrates   Mushroom-Hut Liquid Cultures   Original Sensible Seeds Bulk Cannabis Seeds


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