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InvisibleJHOVA
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Registered: 02/17/17
Posts: 4,727
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Re: Cultivation General Discussion [Re: Edmunter]
    #25739330 - 01/11/19 11:58 PM (5 years, 18 days ago)

im not saying he has bacteria. if he does bacteria can be characterized by wet glossy grains and stalled mycellium growth.


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OfflineAsuraS
Cyantist


Registered: 08/01/11
Posts: 5,047
Loc: Texas
Last seen: 11 days, 23 hours
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Re: Cultivation General Discussion [Re: JHOVA]
    #25739338 - 01/12/19 12:06 AM (5 years, 18 days ago)

Quote:

JHOVA said:
im not saying he has bacteria. if he does bacteria can be characterized by wet glossy grains and stalled mycellium growth.




IMO this one is a tough call. Moisture content is off. Doesn't look bacterial to me...but I have a shit eye for
that compared to some. Weak myc, bad genetics?


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OfflinePyrotek
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Registered: 06/02/18
Posts: 44
Last seen: 4 years, 4 months
Re: Cultivation General Discussion [Re: Asura]
    #25739353 - 01/12/19 12:39 AM (5 years, 18 days ago)

Ok, I'm freaking out a little bit here as I must have some kind of full blown contamination in my place. I was about to do some agar transfers so I got out a shoebox that I've stored some clean pasty plates in. Got them out and pulled off the foil from the lids. Under one of the peices of foil was this white powdery substance that kind of just turned into dust when I accidentally touched it. I realized quickly that I had an issue on my hands and tried to isolate it and get it outside. This was growing on the outside of a pasty plate directly on the plastic. It was below the foil wrapped around the top and side. And was sitting inside a dry shoe box away from any source of moisture or nutrition. Look closely at the photo and you'll notice little yellow dots in that shit. DUDE WTF!



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OfflineThedillestpickle
cultured
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Registered: 02/02/16
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Re: Cultivation General Discussion [Re: Pyrotek]
    #25739387 - 01/12/19 01:31 AM (5 years, 18 days ago)

Got a question here. 

I tossed my old master jar for a culture I, in retrospect, want to keep around. 

I've had this strain going for about a year now and it's been through a lot of transfers. 
Is there any issue with cloning a fruit from one of my tubs?  I originally obtain this culture
by cloning a fruit.  So this would have been transferred many times, cloned, transferred many times etc. 
Am I getting anywhere close to senescence, or is that years away still?


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InvisibleCaps McGee
Grandaddy Smurfshack
I'm a teapot User Gallery


Registered: 10/28/17
Posts: 14,357
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Re: Cultivation General Discussion [Re: Thedillestpickle]
    #25739436 - 01/12/19 03:06 AM (5 years, 18 days ago)

Depends on several factors,  but mostly cell division
.. are you working from the same cold storage culture for expansion, or just been plate to plate at room temp?  A clone will still contain multiple strains, so each transfer or clone  further isolates genetics, and if you're lucky,  you'll end up with a well performing, truly isolated strain... though 11 of 12 isolates fall short of the original multistrain monoculture IME... so in short

"Depends "


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OfflineThedillestpickle
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Re: Cultivation General Discussion [Re: Caps McGee]
    #25739470 - 01/12/19 04:15 AM (5 years, 18 days ago)

Interesting.  Mostly cold stored but sometimes I'd take a new transfer.  I think I will clone it but also germinate some more plates
and start comparing cultures.  I don't think there was anything especially great about this particular culture anyways, it was just very consistent. I just don't want to keep an old culture going if it's getting tired and consistent starts to become consistently lower performance.


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InvisibleCaps McGee
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Registered: 10/28/17
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Re: Cultivation General Discussion [Re: Thedillestpickle]
    #25740029 - 01/12/19 12:08 PM (5 years, 18 days ago)

Take prints and start over


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InvisiblebodhisattaMDiscordReddit
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Re: Cultivation General Discussion [Re: JHOVA]
    #25740082 - 01/12/19 12:40 PM (5 years, 18 days ago)

Quote:

JHOVA said:
Quote:

Pyrotek said:
Can anyone tell me what kind of contamination I'm looking at here? Cuz whatever it is, I got it bad.






it looks ok from the one picture. do you see any wet looking spots pressed against the glass?



How about the fact theres two completely different species of mold growing in there lol


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OfflineEdmunter
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Re: Cultivation General Discussion [Re: bodhisatta]
    #25740120 - 01/12/19 01:03 PM (5 years, 18 days ago)

Has anyone actually made a thread about different contamination in jars and how to spot them?


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InvisiblebodhisattaMDiscordReddit
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Re: Cultivation General Discussion [Re: Edmunter] * 1
    #25740126 - 01/12/19 01:06 PM (5 years, 18 days ago)

You need a microscope to do identification of contaminats otherwise its mostly guess work. Doesn't matter what contamination you get its a toss situation. Even with a scope you cant narrow it down that well especially in the case of bacteria

Spotting them is just as easy as looking tho. You eventually come to realize not every white growth is what you want


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OfflinePsilosopherr
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Re: Cultivation General Discussion [Re: Edmunter] * 1
    #25740159 - 01/12/19 01:26 PM (5 years, 18 days ago)

Quote:

Edmunter said:
Has anyone actually made a thread about different contamination in jars and how to spot them?



there's "how it should and shouldn't look" by tmethyl


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OfflineEdmunter
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Re: Cultivation General Discussion [Re: Psilosopherr] * 1
    #25740177 - 01/12/19 01:39 PM (5 years, 18 days ago)



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OfflinePyrotek
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Registered: 06/02/18
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Re: Cultivation General Discussion [Re: bodhisatta]
    #25740447 - 01/12/19 04:43 PM (5 years, 18 days ago)

Quote:

bodhisatta said:
Quote:

JHOVA said:
Quote:

Pyrotek said:
Can anyone tell me what kind of contamination I'm looking at here? Cuz whatever it is, I got it bad.






it looks ok from the one picture. do you see any wet looking spots pressed against the glass?



How about the fact theres two completely different species of mold growing in there lol




How can you tell there's two different species of mold? I figured it was mold but I don't know what to look for. I knew it wasn't healthy mycelium, though.


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OfflinePyrotek
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Registered: 06/02/18
Posts: 44
Last seen: 4 years, 4 months
Re: Cultivation General Discussion [Re: Pyrotek]
    #25740454 - 01/12/19 04:46 PM (5 years, 18 days ago)

Quote:

Pyrotek said:
Ok, I'm freaking out a little bit here as I must have some kind of full blown contamination in my place. I was about to do some agar transfers so I got out a shoebox that I've stored some clean pasty plates in. Got them out and pulled off the foil from the lids. Under one of the peices of foil was this white powdery substance that kind of just turned into dust when I accidentally touched it. I realized quickly that I had an issue on my hands and tried to isolate it and get it outside. This was growing on the outside of a pasty plate directly on the plastic. It was below the foil wrapped around the top and side. And was sitting inside a dry shoe box away from any source of moisture or nutrition. Look closely at the photo and you'll notice little yellow dots in that shit. DUDE WTF!






Has anyone ever seen anything like this growing on the outside of any of their containers? This one had just come out of the pressure cooker and went straight into a shoebox I use for storage.


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InvisibleCaps McGee
Grandaddy Smurfshack
I'm a teapot User Gallery


Registered: 10/28/17
Posts: 14,357
Loc: ally known as ... Flag
Re: Cultivation General Discussion [Re: Pyrotek]
    #25740480 - 01/12/19 05:04 PM (5 years, 18 days ago)

I dont use no-pours... how long was it stored?


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OfflineDuhrwood Stevens
Gauche Chemoorganoheterotroph
Male


Registered: 09/28/18
Posts: 410
Loc: Allupin Sideya
Last seen: 3 years, 1 month
Re: Cultivation General Discussion [Re: Pyrotek]
    #25740533 - 01/12/19 05:36 PM (5 years, 18 days ago)

Quote:

Pyrotek said:
Quote:

Pyrotek said:
Ok, I'm freaking out a little bit here as I must have some kind of full blown contamination in my place. I was about to do some agar transfers so I got out a shoebox that I've stored some clean pasty plates in. Got them out and pulled off the foil from the lids. Under one of the peices of foil was this white powdery substance that kind of just turned into dust when I accidentally touched it. I realized quickly that I had an issue on my hands and tried to isolate it and get it outside. This was growing on the outside of a pasty plate directly on the plastic. It was below the foil wrapped around the top and side. And was sitting inside a dry shoe box away from any source of moisture or nutrition. Look closely at the photo and you'll notice little yellow dots in that shit. DUDE WTF!






Has anyone ever seen anything like this growing on the outside of any of their containers? This one had just come out of the pressure cooker and went straight into a shoebox I use for storage.



Maybe agar boiled over, got on the outside and started some growth? Just guessing.:goodluck:


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InvisibleCaps McGee
Grandaddy Smurfshack
I'm a teapot User Gallery


Registered: 10/28/17
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Re: Cultivation General Discussion [Re: Duhrwood Stevens]
    #25740555 - 01/12/19 05:45 PM (5 years, 18 days ago)

Very likely


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InvisibleYesum
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Registered: 11/05/12
Posts: 13,124
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Re: Cultivation General Discussion [Re: Pyrotek]
    #25740567 - 01/12/19 06:00 PM (5 years, 18 days ago)

Quote:

Pyrotek said:
Quote:

Pyrotek said:
Ok, I'm freaking out a little bit here as I must have some kind of full blown contamination in my place. I was about to do some agar transfers so I got out a shoebox that I've stored some clean pasty plates in. Got them out and pulled off the foil from the lids. Under one of the peices of foil was this white powdery substance that kind of just turned into dust when I accidentally touched it. I realized quickly that I had an issue on my hands and tried to isolate it and get it outside. This was growing on the outside of a pasty plate directly on the plastic. It was below the foil wrapped around the top and side. And was sitting inside a dry shoe box away from any source of moisture or nutrition. Look closely at the photo and you'll notice little yellow dots in that shit. DUDE WTF!






Has anyone ever seen anything like this growing on the outside of any of their containers? This one had just come out of the pressure cooker and went straight into a shoebox I use for storage.




Well it's healthy mold mycelium lol. Just playing


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OfflineEdmunter
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Re: Cultivation General Discussion [Re: Yesum]
    #25741265 - 01/13/19 04:52 AM (5 years, 17 days ago)

How are these looking.  Would you use them to inoculate jars?

A cubensis I Iso'd the name off of weeks ago probably a clone




PE culture



Alt#7



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InvisibleMadHatter333
We Are All Mad Here

Registered: 09/20/17
Posts: 4,650
Loc: Your Mom’s Rabbit Hole
Re: Cultivation General Discussion [Re: Edmunter]
    #25741552 - 01/13/19 08:48 AM (5 years, 17 days ago)

Quote:

Edmunter said:
How are these looking.  Would you use them to inoculate jars?

A cubensis I Iso'd the name off of weeks ago probably a clone




PE culture



Alt#7






What transfer are they on? Looks like you are getting mostly tomentos mycelium. I usually go for rhizo growth personally, but I hear people have good success with tomentos growth.


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