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JHOVA
Post whore


Registered: 02/17/17
Posts: 4,727
Loc:
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Re: Cultivation General Discussion [Re: Steevo]
#25719015 - 01/02/19 02:58 PM (5 years, 28 days ago) |
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I was thinking about stabbing a bag directly and taping over the hole. No ship or port.
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Caps McGee
Grandaddy Smurfshack



Registered: 10/28/17
Posts: 14,357
Loc: ally known as ...
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Re: Cultivation General Discussion [Re: JHOVA]
#25719021 - 01/02/19 02:59 PM (5 years, 28 days ago) |
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I use led bulbs yes
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Failboat
Fuck Up

Registered: 02/01/18
Posts: 8,736
Last seen: 11 hours, 8 minutes
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Re: Cultivation General Discussion [Re: JHOVA]
#25719027 - 01/02/19 03:01 PM (5 years, 28 days ago) |
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Quote:
JHOVA said: I was thinking about stabbing a bag directly and taping over the hole. No ship or port.
I have tried and failed. I have read of success.
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Bumholio
What's the craic



Registered: 07/23/18
Posts: 4,269
Loc: Shroomsville
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Re: Cultivation General Discussion [Re: JHOVA]
#25719032 - 01/02/19 03:03 PM (5 years, 28 days ago) |
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Quote:
JHOVA said: I was thinking about stabbing a bag directly and taping over the hole. No ship or port.
I've done it and it colonized no problem, I did get contams in the bag though but it was right at the top nowhere near where I stabbed the bag. I'd say that was due to me not sterilizing properly or the seal on the bag failing
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 "great things may come to those who wait, but only what's left by those who hustle"
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butterflyaway
Kaleidoscope Queen



Registered: 10/26/18
Posts: 114
Loc:
Last seen: 3 years, 5 months
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Re: Cultivation General Discussion [Re: juniperus]
#25719761 - 01/02/19 07:52 PM (5 years, 28 days ago) |
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Quote:
sporealchemy said: They should continue at regular pace after the temps come back up. Ppl refrigerate grain masters all the time! 
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juniperus said:
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butterflyaway said: Hi there,
I saw someone asked a question about low temps on this chain but I didn’t see a specific answer. I’ve been away for a few days and it got really cold in my house - about 48 degrees. I knocked up some jars before I left and am wondering if there is any adverse reaction to the colonization process or what will they respond when the temperatures reach the right level?
As far as I understand, colonization is just going to slow down proportionately when temps are sub 65 F. Cube mycelium will not tolerate freezing temperatures, but above that it should survive. Upon returning to higher temperatures the mycelium should respond with increased vigor and colonizing speed. I'm sure someone with more experience would likely give a more informed opinion.
Thanks for the feedback - hoping for some good stuff to happen.
-------------------- There is a better way. Finding the way — well that's the journey
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RandyFeller
EagerBeaver



Registered: 12/18/18
Posts: 185
Loc: PNW
Last seen: 2 years, 10 months
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Re: Cultivation General Discussion [Re: Bumholio]
#25719792 - 01/02/19 08:08 PM (5 years, 28 days ago) |
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Quote:
Bumholio said:
Quote:
JHOVA said: I was thinking about stabbing a bag directly and taping over the hole. No ship or port.
I've done it and it colonized no problem, I did get contams in the bag though but it was right at the top nowhere near where I stabbed the bag. I'd say that was due to me not sterilizing properly or the seal on the bag failing 
I have 6 spawn bags filled with 5qts each colonizing nicely. 2 of them are a day or 2 away from spawning to coir. I stabbed straight thru the bag in my SAB and so far all is well. I wiped the spot on the spawn bag with alcohol 1st, flamed needle and inoculated with LC syringe. Afterwards I put a triple layer of micropore tape over the hole. My bags have the 5 micron patch so I almost used regular packaging or duct tape since I don't think I need more GE/FAE. Good luck.
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growlingmycelium
/@_@/



Registered: 04/15/16
Posts: 346
Loc: Durkadurkastan
Last seen: 7 months, 20 days
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Re: Cultivation General Discussion [Re: JHOVA]
#25719808 - 01/02/19 08:14 PM (5 years, 28 days ago) |
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Quote:
JHOVA said: Is the safest way to noc bags in a sab injecting lc via syringe or g2g?
I personally have a piece of duct tape or masking tape on standby and just go right through the plastic, tape it up immediately, flame and repeat. Works 99% of the time if I have clean LC or MS. Dont see a need for the ports honestly.
(warning not an experienced opinion lol)
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JHOVA
Post whore


Registered: 02/17/17
Posts: 4,727
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word. im gonna try it out. i gotta make josex fancy LC lids asap.
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Josex said:
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pacmanbreed said: Cant sleep, waiting for no pour/ no aspirate tek of brother josex. was guessing that involves using pressure to push the liquid out? 
I'm not gonna lie to you, I've been using syringes lately to aspirate from a jar without a ship, only because I was kinda forced to it, had lots of mold from pouring due to my place being totally fucked with mold. I can tell you one thing, they are safer than pouring but I don't like aspirating one bit because it's a huge hassle, although I have to admit once you have your LC safely into syringes that shit is gold.
This is what I did, which is not what I said I'd do in the future btw.


And that got me some nice 60 ml syringes full of magic. 

These are the steps in case anyone wants to give it a try, I'm not gonna do a tek cause that's phoney, I don't really like this shit, but I don't want it to go unnoticed either, so I'll make a quick tek here for anyone who might find it interesting:
 The lids of these little jelly jars happen to a tad smaller than the lids from a regular wide mouth mason jar, you'll see why.
 Apply epoxy glue on the edges of the jelly jar lid.
 Glue the lid of the jelly jar to the lid of the wide mouth mason jar.
 Make a tiny hole through both lids using only the tip of a turkey flavor needle, use a hammer, don't go all the way through, just the tip.
 Cut the tip of the turkey needle.
 Make a clean bigger hole through both lids.
 Get some stainless steel tubes (4mm dia x 0.30 wall) and cut them the lenght of jar (155 mm worked for me, so that the tube reaches the bottom of the jar).
 Inser the turkey needle you cut through the hole (it must fit tight with the help of a few light hits with a hammer) and cover it with epoxy.
 Insert stainless steel tube cut to length in the needle.
 Appy glue to the hub of the needle to secure it even more.
 Make a hole through the bottom of the little jelly jar. You have to use a glass drill bit for this (cheap), do the hole under water, glass dust is not a fun time.
  Glue a piece of SFD to the jar so that it covers the hole (you can skip the SFD and simply cover the hole with several layers of MP tape). You need some form of GE, not because myc really needs it but because you want to avoid creating a vacuum in the jar during the pc cycle. If you don't make a GE hole, next time you open the jar it will suck up tons of nasty air into it.
 Cut the tip of a small syringe and fill it with silicone.
  Prepare the broth for the LC, cover the big hole with 3 layers of MP tape.
 This is the inoculation hole but it also serves as a filter when you aspirate the LC (so that air from the exterior will be sucked in as you aspirate). Do not do the small piece of foil as explained in the OP for this, that won't allow enough air in when you aspirate and it's not gonna let you aspirate normally.
Cover that shit with the jelly jar and pc.

To inoculate, simply open the jelly jar with one hand, stab the mp tape with the other and squirt the tissue, close the jar.
When it's time to aspirate, put another layer of MP tape over the inoculation hole. unscrew the syringe tip filled with silicone from the turkey needle, screw sterile syringe right from the package (or sterilize it yourself) in the turkey needle. When it's full, leave it like that, prepare the next syringe, unscrew the filled syringe and quickly insert the next syringe, screw a needle in the filled syringe. Rinse, repeat, till there's no LC left in the jar.

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Nimph
Im Unclecaptainblue!!!!

Registered: 08/12/18
Posts: 1,605
Loc: The dirty
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Re: Cultivation General Discussion [Re: JHOVA]
#25720022 - 01/02/19 10:32 PM (5 years, 28 days ago) |
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I saw nothing hehe, whos played around with that?
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Thedillestpickle
cultured



Registered: 02/02/16
Posts: 1,170
Loc: Canada
Last seen: 3 years, 7 months
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I do things quite differently than most here.
I don't seal my bags before PC and I don't use Tyvek sleeves or anything. Just twist up the top and fold it over. weight down the twisted tops of the bags with a dinner plate. After PC cycle and in front of a flow hood I untwist the bag, and shoot 60 mL of LC into the bag. Then seal. Then shake. First signs of colonization within 2 or 3 days and usually fully colonized in just over a week.
The same should work in a SAB but I've never tested it.
As for the LC I also have my own recipe. I make 2 liter batches of LC, enough to inoculate 24 bags with a bit left over. I use a 4L pickle jar. I add 5 grams agar agar per 2 liters liquid, which allows the LC to be shaken and small bubbles will suspend in the liquid which helps create a better LC. I highly recommend this to anyone working with LC, although it is important that you have a proper vessel that will allow for vigorous shaking without increasing the risk of contamination.
Also for cleaning syringes... Just pressure cook them for 30 minutes and they are good to go. My syringes have been used dozens of times in this way and they work just fine.
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Thedillestpickle
cultured



Registered: 02/02/16
Posts: 1,170
Loc: Canada
Last seen: 3 years, 7 months
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That is one very complicated Tek by Josex

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TheMadHatter420
Trusted Farmer


Registered: 10/12/16
Posts: 12,941
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I tried the LC thing for a while and I couldn't get consistent results. I opted for a 100mm agar plate to be cut into 4 wedges for 4 masters. Those 4 masters could theoretically inoculate 40 more jars. BUT I just dump one master in a bag nice and fast and seal up the bag. So 1 agar plate ends up giving me 4 large mono tubs. I could expand those masters to 40 jars, and those into 40 bags for 40 tubs. My success rate on tubs and jars ahs to be over 95%. I RARELY loose a tub before 1st flush, I think I only had one week out of the last 4 months that I lost any, and I lost 6 from a bad spawn run. Other than those 6 tubs, from that one run I aint lost shit.
As for masters and bags, they have to be 95%+ as well. I rarely loose a bag as if something is wrong, I usually catch it in the master jar.
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juniperus
Frim Fram Sauce


Registered: 12/02/18
Posts: 718
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Quote:
TheMadHatter420 said: I tried the LC thing for a while and I couldn't get consistent results. I opted for a 100mm agar plate to be cut into 4 wedges for 4 masters. Those 4 masters could theoretically inoculate 40 more jars. BUT I just dump one master in a bag nice and fast and seal up the bag. So 1 agar plate ends up giving me 4 large mono tubs. I could expand those masters to 40 jars, and those into 40 bags for 40 tubs. My success rate on tubs and jars ahs to be over 95%. I RARELY loose a tub before 1st flush, I think I only had one week out of the last 4 months that I lost any, and I lost 6 from a bad spawn run. Other than those 6 tubs, from that one run I aint lost shit.
As for masters and bags, they have to be 95%+ as well. I rarely loose a bag as if something is wrong, I usually catch it in the master jar.
For the ones you lost, you spawned contaminated grains by accident? Any idea what it was? I know some contams are harder to spot in the master. Just curious.
-------------------- To avoid all evil, to cultivate good, and to cleanse one's mind - this is the teaching of the Buddhas
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JHOVA
Post whore


Registered: 02/17/17
Posts: 4,727
Loc:
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Re: Cultivation General Discussion [Re: juniperus]
#25720788 - 01/03/19 11:05 AM (5 years, 27 days ago) |
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MH when you dump a jar into a bag do you avoid having the jar make contact with the inner bag?
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hamonz
Junglist



Registered: 09/07/18
Posts: 178
Last seen: 6 months, 28 days
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Re: Cultivation General Discussion [Re: JHOVA]
#25721094 - 01/03/19 02:04 PM (5 years, 27 days ago) |
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I have a shoebox that I spawned on 1/1, one of the brf cakes were pinning all over. the shoebox already had a little fruit growing! What should I do?
I plan on not touching the shoebox for 10 days, just leave it be.
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growlingmycelium
/@_@/



Registered: 04/15/16
Posts: 346
Loc: Durkadurkastan
Last seen: 7 months, 20 days
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Re: Cultivation General Discussion [Re: hamonz]
#25721161 - 01/03/19 02:42 PM (5 years, 27 days ago) |
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I think.... I'm gonna go from 30 Jars of RW and GT to 240 VT pp5 containers... Found a superrr good deal on them locally
@_@
I cant stop myself.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Cultivation General Discussion [Re: JHOVA] 1
#25721163 - 01/03/19 02:44 PM (5 years, 27 days ago) |
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Quote:
JHOVA said: MH when you dump a jar into a bag do you avoid having the jar make contact with the inner bag?
I would. If it touches the inner bag I mark it as sloppy and keep an eye on it.
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ShaperDreaming
Weirdo



Registered: 10/30/18
Posts: 3,429
Loc: United States
Last seen: 2 years, 3 days
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Re: Cultivation General Discussion [Re: hamonz]
#25721199 - 01/03/19 03:10 PM (5 years, 27 days ago) |
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Scalpels... So, what do ya'll use? I'm tired of using disposable scalpels because they're not cheap for how often I use them, but can do them... but like... carbon on the blade from flame sterilizing is really messing up some of my agar work. I know in theory it should be sterile, but it always grows shit if anything drops off the blade when "cooling" it by slicing into agar.
Quote:
Pastywhyte said: If it touches the inner bag I mark it as sloppy and keep an eye on it.
I put frowny faces on all of these types of grows.
Edited by ShaperDreaming (01/03/19 03:11 PM)
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Subfinder
Wildlife enthusiast


Registered: 07/01/18
Posts: 1,025
Loc: Pennsylvania
Last seen: 9 months, 19 days
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Quote:
ShaperDreaming said: Scalpels... So, what do ya'll use? I'm tired of using disposable scalpels because they're not cheap for how often I use them, but can do them... but like... carbon on the blade from flame sterilizing is really messing up some of my agar work. I know in theory it should be sterile, but it always grows shit if anything drops off the blade when "cooling" it by slicing into agar.
Quote:
Pastywhyte said: If it touches the inner bag I mark it as sloppy and keep an eye on it.
I put frowny faces on all of these types of grows.
Use a propane torch...leaves no carbon
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mushroomnate
Pstranger



Registered: 05/17/17
Posts: 3,100
Last seen: 2 days, 12 hours
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Quote:
ShaperDreaming said: Scalpels... So, what do ya'll use? I'm tired of using disposable scalpels because they're not cheap for how often I use them, but can do them... but like... carbon on the blade from flame sterilizing is really messing up some of my agar work. I know in theory it should be sterile, but it always grows shit if anything drops off the blade when "cooling" it by slicing into agar.
Quote:
Pastywhyte said: If it touches the inner bag I mark it as sloppy and keep an eye on it.
I put frowny faces on all of these types of grows.
I got this dissection kit awhile back for $15 or so on amazon,came with a #3 scalpel handle,11 blades and alot of extra fun stuff to play with. .
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