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Shmurf
Spaceman


Registered: 02/20/17
Posts: 133
Loc: The Ether
Last seen: 4 years, 10 months
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Re: Cultivation General Discussion [Re: bodhisatta]
#25689362 - 12/19/18 11:43 AM (5 years, 1 month ago) |
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Thoughts on this tub? I've noticed what appears like knotting in some areas, while others are a bit "fuzzy".
Do I need to up the FAE, and reduce the misting?
-------------------- Sincerely, Spaceman Shmurf
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Brain Bulb
Insane in the Membrane



Registered: 11/09/17
Posts: 1,358
Last seen: 6 months, 24 days
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Re: Cultivation General Discussion [Re: Shmurf]
#25689465 - 12/19/18 12:43 PM (5 years, 1 month ago) |
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I’ll let them go for a couple more days to see what happens. I should have tested the LI first... Even though the plates looked good.
I’ve read that rye seed is a better medium to spawn Caerulescens, but at the same time it’s more prone to contaminants. We’ll see.
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TheBlackCat
Possibly Human



Registered: 10/05/16
Posts: 2,819
Last seen: 1 year, 5 months
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Re: Cultivation General Discussion [Re: Brain Bulb]
#25689481 - 12/19/18 12:55 PM (5 years, 1 month ago) |
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I'm not familiar with that species but I do think you might be growing a big jar of mold. You could always loosen the lid slightly and without opening give it a smell. If there's that much mold you don't even have to fully open the jar to smell it nor would I recommend opening it.
Quote:
Brain Bulb said: I’ll let them go for a couple more days to see what happens. I should have tested the LI first... Even though the plates looked good.
I’ve read that rye seed is a better medium to spawn Caerulescens, but at the same time it’s more prone to contaminants. We’ll see.
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Brain Bulb
Insane in the Membrane



Registered: 11/09/17
Posts: 1,358
Last seen: 6 months, 24 days
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Re: Cultivation General Discussion [Re: Brain Bulb]
#25689482 - 12/19/18 12:56 PM (5 years, 1 month ago) |
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Have to say that ever since I changed the agar recipe I use around a bit I’ve been getting much better cultures. I tried a straight MEA but it was shit. Maybe I didn’t use the right ratios but the recipe I found was only for 300 mL and I do 400 mL batches. I increased the ingredients for a 400 mL bottle but transfers look horrible. Used 7g agar ~ 7g LME.
Side by side comparison of the GSMEA I’ve been using with the MEA. This is my Cambodian clone both transfers are from the same plate at a couple seconds apart.
GSMEA:

MEA:
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butterflyaway
Kaleidoscope Queen



Registered: 10/26/18
Posts: 114
Loc:
Last seen: 3 years, 5 months
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Re: Cultivation General Discussion [Re: TheBlackCat]
#25689485 - 12/19/18 12:57 PM (5 years, 1 month ago) |
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If jars of substrate fail to colonize, can you reuse the substrate jars or should I just start from scratch again?
-------------------- There is a better way. Finding the way — well that's the journey
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
Last seen: 3 days, 11 hours
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I reuse moldy jars without issue. I always wash them first but itll be pcd anyway
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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butterflyaway
Kaleidoscope Queen



Registered: 10/26/18
Posts: 114
Loc:
Last seen: 3 years, 5 months
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Re: Cultivation General Discussion [Re: tryptkaloids]
#25689510 - 12/19/18 01:06 PM (5 years, 1 month ago) |
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Quote:
tryptkaloids said: I reuse moldy jars without issue. I always wash them first but itll be pcd anyway
Sorry, I meant reuse the substrate that is already in the jars, if they produced nothing. They haven't colonized and its been over 3 weeks.
-------------------- There is a better way. Finding the way — well that's the journey
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
Last seen: 3 days, 11 hours
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Pics
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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Bumholio
What's the craic



Registered: 07/23/18
Posts: 4,269
Loc: Shroomsville
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Re: Cultivation General Discussion [Re: tryptkaloids]
#25689529 - 12/19/18 01:17 PM (5 years, 1 month ago) |
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Quote:
butterflyaway said: If jars of substrate fail to colonize, can you reuse the substrate jars or should I just start from scratch again?
I prolly wouldn't, theres a reason they didn't colonize
--------------------
 "great things may come to those who wait, but only what's left by those who hustle"
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
Last seen: 3 days, 11 hours
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Re: Cultivation General Discussion [Re: Bumholio]
#25689538 - 12/19/18 01:21 PM (5 years, 1 month ago) |
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That reason is likely from moisture problems. It's possible he can still spawn some of it. That's why i want pics
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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butterflyaway
Kaleidoscope Queen



Registered: 10/26/18
Posts: 114
Loc:
Last seen: 3 years, 5 months
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Re: Cultivation General Discussion [Re: Bumholio]
#25689540 - 12/19/18 01:22 PM (5 years, 1 month ago) |
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Quote:
Bumholio said:
Quote:
butterflyaway said: If jars of substrate fail to colonize, can you reuse the substrate jars or should I just start from scratch again?
I prolly wouldn't, theres a reason they didn't colonize 
Right, I think they were either too dry or too warm.. but don't know which. I'll start from scratch then. @ least I got 50% of my jars to colonize.
-------------------- There is a better way. Finding the way — well that's the journey
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Bumholio
What's the craic



Registered: 07/23/18
Posts: 4,269
Loc: Shroomsville
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Re: Cultivation General Discussion [Re: tryptkaloids]
#25689544 - 12/19/18 01:24 PM (5 years, 1 month ago) |
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Maybe so, but would you resterilize grain that's been sitting in a jar for 3 weeks or prep some more?
--------------------
 "great things may come to those who wait, but only what's left by those who hustle"
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Duhrwood Stevens
Gauche Chemoorganoheterotroph



Registered: 09/28/18
Posts: 410
Loc: Allupin Sideya
Last seen: 3 years, 1 month
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Re: Cultivation General Discussion [Re: Bumholio]
#25689547 - 12/19/18 01:26 PM (5 years, 1 month ago) |
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Quote:
Bumholio said:
Quote:
butterflyaway said: If jars of substrate fail to colonize, can you reuse the substrate jars or should I just start from scratch again?
I prolly wouldn't, theres a reason they didn't colonize 
Sub or grain? Either one can likely be PC'd again as long as moisture is right... maybe??? That said, why not just throw away fails and try to apply what we learned toward the next attempt?
-------------------- Last seen 12/2/2020
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
Last seen: 3 days, 11 hours
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Re: Cultivation General Discussion [Re: Bumholio]
#25689553 - 12/19/18 01:31 PM (5 years, 1 month ago) |
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Quote:
Bumholio said: Maybe so, but would you resterilize grain that's been sitting in a jar for 3 weeks or prep some more?
As long as he dries it and repreps it should be fine.
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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bongoman
Stranger

Registered: 09/19/18
Posts: 558
Last seen: 7 days, 2 hours
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Re: Cultivation General Discussion [Re: tryptkaloids]
#25689584 - 12/19/18 01:49 PM (5 years, 1 month ago) |
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This is a week after a G2G transfer and one shake along the way.
Everything is colonising nice and evenly except for that one patch in one jar.
Maybe I just didn't shake it as well as the others?
Do I need to be concerned about it?
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
Last seen: 3 days, 11 hours
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Re: Cultivation General Discussion [Re: bongoman]
#25689596 - 12/19/18 01:54 PM (5 years, 1 month ago) |
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Yes. No.
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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Nimph
Im Unclecaptainblue!!!!

Registered: 08/12/18
Posts: 1,605
Loc: The dirty
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Re: Cultivation General Discussion [Re: tryptkaloids]
#25689638 - 12/19/18 02:07 PM (5 years, 1 month ago) |
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You mean SHE....rascit...
Bad jars either get cleaned before it gets to bad or i throw them away cause a bacterial jar thats been getting funky can make some people puke or even sick.
--------------------
  Hunters save your exotic prints to trade! https://www.shroomery.org/forums/showflat.php/Number/25617539
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Duhrwood Stevens
Gauche Chemoorganoheterotroph



Registered: 09/28/18
Posts: 410
Loc: Allupin Sideya
Last seen: 3 years, 1 month
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Re: Cultivation General Discussion [Re: Nimph]
#25689672 - 12/19/18 02:30 PM (5 years, 1 month ago) |
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Top definition on Urban Dictionary rascit cool, great, badass like 'bangin' or 'dope'. related to a rascal Man, you should have gone to the concert, it was rascit!
That verse was rascit! Spit it again!
I heard he is always behaving rascit; getting into trouble all the time.
-------------------- Last seen 12/2/2020
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Edmunter
Mr



Registered: 05/01/13
Posts: 5,699
Last seen: 19 days, 7 hours
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Re: Cultivation General Discussion [Re: Brain Bulb]
#25689801 - 12/19/18 03:43 PM (5 years, 1 month ago) |
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Quote:
Brain Bulb said: How long do I need to let the GS inoculated with LC incubate? This jar is fully colonized with light growth with patches of thicker myc. Do I wait til the entire jar has nice thick myc?

Mycelium thats not cubensis can look like this. Best talk to someone with experience like Chronic. Cubensis growers dont really understand other mycelium. When u open just give it a good sniff.
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Asura
Cyantist


Registered: 08/01/11
Posts: 5,047
Loc: Texas
Last seen: 12 days, 49 minutes
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Re: Cultivation General Discussion [Re: Edmunter]
#25689808 - 12/19/18 03:46 PM (5 years, 1 month ago) |
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Looks just like pans are supposed to look. Give it a sniff. Should smell like almonds/almond extract. That will be your true indicator.
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