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Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Cultivation General Discussion [Re: Brain Bulb]
#25639175 - 11/25/18 09:07 PM (5 years, 2 months ago) |
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I made a diy soda steam i carbonated a bunch of lemonade and then added bullet bourbon. It's like whisky sour but better. Plus rumchata or however its spelled. Some high life. And my buddy brought sake.
Everyone should have a home carbonator
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Cultivation General Discussion [Re: bodhisatta]
#25639197 - 11/25/18 09:22 PM (5 years, 2 months ago) |
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Holy fuck mason bill cosby actually made some points
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Psilosopherr
A psilly goose



Registered: 02/15/12
Posts: 12,278
Last seen: 1 month, 10 days
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Re: Cultivation General Discussion [Re: bodhisatta]
#25639240 - 11/25/18 09:48 PM (5 years, 2 months ago) |
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random question: Has anybody tried starting outdoor patches with sclerotia producing species? Or seen posts where people have.
other than steve-o
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Tookitooki
Mycological Fabricator


Registered: 07/28/16
Posts: 1,157
Loc: Nowhere
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Re: Cultivation General Discussion [Re: ComebackKid]
#25639311 - 11/25/18 10:34 PM (5 years, 2 months ago) |
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Congrats cbk!! Just noticed.
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sh4d0ws
LSx


Registered: 02/26/08
Posts: 12,086
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Re: Cultivation General Discussion [Re: Tookitooki]
#25639481 - 11/26/18 03:04 AM (5 years, 2 months ago) |
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Has anyone seen HEPA filters that are only 7/8" thick? I need to clarify with the guy but I'm starting to wonder if he means it's available in 7 or 8 inches.
The resistance is 1 W.G.
Got quoted at $325 for a 99.99% efficient 18" * 24" (by whatever thickness, 7/8 inch or 7-8 inches, or 7 or 8 inches lol...)
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crystalknight
Mycellium



Registered: 05/13/16
Posts: 54
Last seen: 3 years, 6 months
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Re: Cultivation General Discussion [Re: Tookitooki]
#25639484 - 11/26/18 03:07 AM (5 years, 2 months ago) |
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I have Psilocybe Subaeruginosa mycelium that has colonised woodchips in a glass jar. This species only fruits in autumn and winter. I currently having the jar sitting outside in a shaded area, but it is about to become summer and get really hot. What should I do with it? Move the jar into a cooler area such as under a house? Leave it where it is? Put it in a fridge? release the woodchips into a garden? What will give it the best chance of surviving?
Also, is it a good idea to add moisture to the jar or keep it semi dry?
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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@Brain Bulb
Try this if you have more tissue, transfer it to T-Gel, but with malt extract, no flour (Mycolorado's version).
100g > Water | 2g > Agar | 2g > Black Tea | 1g > Malt Extract. (Assembly in my sig).
Once you have good growth, transfer it to normal agar.

Image provided by Mycolorado.
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@bodhisatta
I guess you could also make calcium bicarbonate from calcium carbonate too.
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
Last seen: 3 days, 8 hours
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Re: Cultivation General Discussion [Re: Ferather]
#25639728 - 11/26/18 08:37 AM (5 years, 2 months ago) |
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Quote:
bodhisatta said:
Quote:
ComebackKid said: It's hard to say with certainty what's going on in that plate. It doesnt look like traditional cube myc growth but it does have some of the traits near the center and around the leading edge.
Its definitely too sketchy for grain at this point but see what happens after a transfer or try whipping up a batch of plates using a different agar recipe and see how it responds on that.
I love seeing that tag on your account

 Quote:
van hatton said:
 
I'mma post this here to.
Quote:
van hatton said: Why do we need spacers for grain steam sterilization but not 15 psi sterilization? Or am I mistaken? Speaking specifically for 5 lb bags.
Or should I just cram it and find out 
Everything I've ever read on how to do bags says to use jar rings between bags to allow steam through and around the bags so the pc doesnt pop em
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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sandman420
Saint PP



Registered: 06/17/04
Posts: 5,384
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Re: Cultivation General Discussion [Re: tryptkaloids]
#25639793 - 11/26/18 09:12 AM (5 years, 2 months ago) |
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Meh, jar rings dont really separate bags at all after it gets hot the bags just meld around them anyway. Its better if you can just stack the bags a little apart. Plus if you put rings between spawn bags when sterilizing it will deform the bag and can sometimes make impulse sealing really hard or unable to properly seal from the ring impression left after.
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sh4d0ws
LSx


Registered: 02/26/08
Posts: 12,086
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Re: Cultivation General Discussion [Re: sh4d0ws]
#25639804 - 11/26/18 09:17 AM (5 years, 2 months ago) |
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Quote:
sh4d0ws said: Has anyone seen HEPA filters that are only 7/8" thick? I need to clarify with the guy but I'm starting to wonder if he means it's available in 7 or 8 inches.
The resistance is 1 W.G.
Got quoted at $325 for a 99.99% efficient 18" * 24" (by whatever thickness, 7/8 inch or 7-8 inches, or 7 or 8 inches lol...)
Nevermind! Fuck it I found an astrocel 99.99 efficient on ebay. It was a decent chunk of change but brand new and no more than I would have paid from a filter supplier. Found a matching inline fan for $110 CAD
Should be here in 2-3 days and then I can get started building this thing
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van hatton
Still a noob


Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Re: Cultivation General Discussion [Re: sh4d0ws]
#25639808 - 11/26/18 09:19 AM (5 years, 2 months ago) |
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Don't use an inline fan for a flowhood.
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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sandman420
Saint PP



Registered: 06/17/04
Posts: 5,384
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Re: Cultivation General Discussion [Re: sh4d0ws]
#25639809 - 11/26/18 09:19 AM (5 years, 2 months ago) |
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They probably meant 5-7/8" which is the standard flowhood HEPA filter thickness.
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
Last seen: 3 days, 8 hours
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Re: Cultivation General Discussion [Re: sandman420]
#25639822 - 11/26/18 09:25 AM (5 years, 2 months ago) |
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Quote:
sandman420 said: Meh, jar rings dont really separate bags at all after it gets hot the bags just meld around them anyway. Its better if you can just stack the bags a little apart. Plus if you put rings between spawn bags when sterilizing it will deform the bag and can sometimes make impulse sealing really hard or unable to properly seal from the ring impression left after.
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Cultivation General Discussion [Re: Ferather] 1
#25639833 - 11/26/18 09:32 AM (5 years, 2 months ago) |
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Quote:
Ferather said: @Brain Bulb
Try this if you have more tissue, transfer it to T-Gel, but with malt extract, no flour (Mycolorado's version).
100g > Water | 2g > Agar | 2g > Black Tea | 1g > Malt Extract. (Assembly in my sig).
why would you use antibiotic/tea agar on(possible) mold? bulb doesnt have bacteria from the culture. he has mold imo..

thats like giving a person antibiotics for the flu.
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sh4d0ws
LSx


Registered: 02/26/08
Posts: 12,086
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Re: Cultivation General Discussion [Re: van hatton]
#25639861 - 11/26/18 09:49 AM (5 years, 2 months ago) |
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Quote:
van hatton said: Don't use an inline fan for a flowhood.
Why? They use one in the fungi fun flow hood build tek
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Mycolorado
Hobbyist


Registered: 07/23/16
Posts: 8,529
Loc: Interdimensional Bootcamp
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Re: Cultivation General Discussion [Re: sh4d0ws]
#25639884 - 11/26/18 10:02 AM (5 years, 2 months ago) |
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You can get away with it if it’s a smaller filter and a larger fan. Pretty sure the VTX series from vortex will work.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Cultivation General Discussion [Re: Mycolorado]
#25639891 - 11/26/18 10:07 AM (5 years, 2 months ago) |
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@mushboy
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Set 1: I see bacteria, and discoloration. Low nutrient mycelium, evasion of areas (which are darker), I do not see any sporulation.

The mycelium also starts to look more normal the further it pushes out.
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Set 2: Same bacteria, same growth, some discoloration. It's overly tomentose and not pushing out.
  
Set 2:
Quote:
Brain Bulb said: This is my other clone plate. Starting to see a little bacteria at the 12 o’clock position so I’m gonna go ahead and take a couple transfers from it as well.
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Quote:
Brain Bulb said: P. Caerulescens - T3 from wild clone
  
Again here I see discoloration more than any spores, and possible oxidation from damage.
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If he is getting mold from tissue like that, I can't see how it fruited at all.
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Cultivation General Discussion [Re: Ferather]
#25639902 - 11/26/18 10:16 AM (5 years, 2 months ago) |
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Quote:
If he is getting mold from tissue like that, I can't see how it fruited at all.
because it grew outdoor dude(wild fruit). do you even shroomery?? and that bacteria on the media is scattered. like satellite colonies from technique. its not growing off the culture.
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sh4d0ws
LSx


Registered: 02/26/08
Posts: 12,086
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Re: Cultivation General Discussion [Re: Mycolorado]
#25639907 - 11/26/18 10:20 AM (5 years, 2 months ago) |
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Quote:
Mycolorado said: You can get away with it if it’s a smaller filter and a larger fan. Pretty sure the VTX series from vortex will work.
I just looked at the fan chart and made sure that the sum resistance (filter resistance + pre-filter resistance) line up with the right CFM for the size of my filter. The inline I got is actually slightly overpowered so I bought a speed adjustment dial.
I guess if it doesn't work I can return it and opt for something bigger but I don't see any reason why it won't
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Cultivation General Discussion [Re: sh4d0ws]
#25640081 - 11/26/18 11:36 AM (5 years, 2 months ago) |
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Set 2:
It looks like growth that is competing with other organisms, likely producing enzymes that decompose the tissue and agar.
Growth is patchy, tomentose and not pushing out, there's discoloration at 6 o'clock of images 2 and 3. The discoloration appears to be bleeding out (probably a colony), and not spores.
Mold should be sporulating, or if it where a pin mold, some aerial pods. There is also slime at 12 o'clock as Brain Bulb has said.
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Here is an infected wood peg (archaea and randoms), with oyster mycelium, onto T-Gel (with flour). The contamination(s) grew out, they competed, and they also produced a brown slime.
Growth started patchy, more tomentose, not pushing well, and sluggish.

The blackening in this case is mostly archaea, not the fungi.
When I swapped the flour for gelatin I escaped the randoms, but not archaea.

No slime, archaea obviously faster than the mycelium.
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Archaea and various other organisms can be on agar, in agar, growing out (even unseen).
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While archaea is not a major issue, I stopped using liquid seaweed mixes. Archaea seems to facilitate the growth of various organisms.
Edited by Ferather (11/26/18 01:07 PM)
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