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Invisiblegrainbrain
Tribalistic

Registered: 05/11/11
Posts: 2,626
Re: Cultivation General Discussion [Re: CzM8]
    #25637927 - 11/25/18 10:17 AM (5 years, 2 months ago)

Quote:

CzM8 said:

:drphil:




:inonothing:


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Stop cold shocking your mycelium!
Hot Spawn - Get It On



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Offlinelewp
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Registered: 06/04/17
Posts: 403
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Re: Cultivation General Discussion [Re: grainbrain]
    #25637971 - 11/25/18 10:36 AM (5 years, 2 months ago)

Do most people cool their scalpel in the donor plate or the receiver?


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Wassup Fresh? It's our turn baby


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OfflineCzM8
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Re: Cultivation General Discussion [Re: lewp]
    #25637974 - 11/25/18 10:37 AM (5 years, 2 months ago)

On outer area of their receiver.


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InvisiblemushboyMDiscord
modboy
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Registered: 04/24/05
Posts: 32,281
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Re: Cultivation General Discussion [Re: lewp]
    #25637981 - 11/25/18 10:43 AM (5 years, 2 months ago)

Quote:

lewp said:
Do most people cool their scalpel in the donor plate or the receiver?




I just take my time. Sterile work isn't a race.
But even professional agar tutorials have you cool in the donor plate.


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OfflineCzM8
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Re: Cultivation General Discussion [Re: mushboy] * 1
    #25637989 - 11/25/18 10:46 AM (5 years, 2 months ago)

Quote:

mushboy said:
Quote:

lewp said:
Do most people cool their scalpel in the donor plate or the receiver?




I just take my time. Sterile work isn't a race.
But even professional agar tutorials have you cool in the donor plate.





Wait, WHAT? I thought I've watched sevearl videos that cool on the receiver plate. My assumption was that the receiver plate is sterile of ALL matter, therefore I want to make sure my scalpel is less likely to get contaminated on a sterile plate vs a plate that has my transfer on it.

Maybe I need to re-watch those videos. But out of 200 plates+, I only have contamination on some germination plates, and 2 transfers.


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InvisibleGerms
Space Force
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Registered: 06/26/11
Posts: 4,607
Loc: Texas
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Re: Cultivation General Discussion [Re: CzM8]
    #25637991 - 11/25/18 10:47 AM (5 years, 2 months ago)


Finally getting some work done for the first time in weeks since my accident


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OfflinecronicrFacebook
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Registered: 08/07/11
Posts: 61,436
Loc: Van Isle Flag
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Re: Cultivation General Discussion [Re: CzM8]
    #25638005 - 11/25/18 10:54 AM (5 years, 2 months ago)

Quote:

CzM8 said:
Quote:

mushboy said:
Quote:

lewp said:
Do most people cool their scalpel in the donor plate or the receiver?




I just take my time. Sterile work isn't a race.
But even professional agar tutorials have you cool in the donor plate.





Wait, WHAT? I thought I've watched sevearl videos that cool on the receiver plate. My assumption was that the receiver plate is sterile of ALL matter, therefore I want to make sure my scalpel is less likely to get contaminated on a sterile plate vs a plate that has my transfer on it.

Maybe I need to re-watch those videos. But out of 200 plates+, I only have contamination on some germination plates, and 2 transfers.



Yes reciever plate is best


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InvisiblemushboyMDiscord
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Re: Cultivation General Discussion [Re: cronicr]
    #25638014 - 11/25/18 10:58 AM (5 years, 2 months ago)

Oops. I ment receiving plate, not donor.

I r tarded :kingtard:


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OfflineFerather
Mycological
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Registered: 03/19/15
Posts: 6,325
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Re: Cultivation General Discussion [Re: mushboy]
    #25638283 - 11/25/18 01:15 PM (5 years, 2 months ago)

Quote:

bodhisatta said:
Quote:

Ferather said:
Brown rot:

My example is Cubensis,



Brown rot only refers to what some wood decay fungi do. Cubes are absolutely not a brown rot fungi lol.




To my understanding, brown-rot is mycelium that will apparently decay cellulose, but do not utilize phenols.
White-rot fungi directly targets phenols for decay and absorption, causing it to whiten.

https://science.energy.gov/ber/highlights/2017/ber-2017-02-e/
https://en.wikipedia.org/wiki/Wood-decay_fungus

There is no chance the Cubensis in my sample is using the trace sucrose solely as the carbon source.
The sample is oxidizing the phenols, turning them darker, but also utilizing them.

In comparison to Oyster, it's behaving as a brown rot fungi.


--------------------
                   

Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

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InvisibleTXBD
StrangerthanFiction
I'm a teapot User Gallery

Registered: 06/24/18
Posts: 261
Loc: Tejas
Re: Cultivation General Discussion [Re: mushboy]
    #25638288 - 11/25/18 01:19 PM (5 years, 2 months ago)

Is this bacterial/contaminated or just a field of aborts being reclaimed by mycelium? This has been in progress of turning white for several days now. I think I over-misted the tubs and caused the aborts but not sure.





Should I remove all of the white blobs/aborts? Cover them with a casing? Throw out the sub and start over?


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I overdo everything and nothing at the same time. Every time, All the time, and some times too.. :bigjoint:


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InvisiblePlayer 1
Don't follow me I'm lost


Registered: 10/09/18
Posts: 53
Loc: Earth
Re: Cultivation General Discussion [Re: TXBD]
    #25638691 - 11/25/18 04:50 PM (5 years, 2 months ago)

When should a monotub be put into fruiting conditions?


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InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
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Re: Cultivation General Discussion [Re: Player 1] * 1
    #25638695 - 11/25/18 04:52 PM (5 years, 2 months ago)

Immediately


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OfflineBrain Bulb
Insane in the Membrane
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Registered: 11/09/17
Posts: 1,358
Last seen: 6 months, 24 days
Re: Cultivation General Discussion [Re: bodhisatta]
    #25638801 - 11/25/18 05:58 PM (5 years, 2 months ago)

I posted this up the other day and it was mentioned the plate was mainly tams.  I forgot to chuck it and was looking at my plates a little while ago.  Since it’s had more time to incubate it’s starting to look more like myc.  What do you guys think.  Still mold?



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OfflineMH5109
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Registered: 09/27/17
Posts: 1,385
Loc: America Flag
Last seen: 2 years, 11 months
Re: Cultivation General Discussion [Re: Brain Bulb] * 1
    #25638807 - 11/25/18 06:06 PM (5 years, 2 months ago)

That still looks weird, it doesn't look like mycelium to me but I'm not an expert.


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InvisiblemushboyMDiscord
modboy
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Posts: 32,281
Loc: where?
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Re: Cultivation General Discussion [Re: MH5109]
    #25638808 - 11/25/18 06:07 PM (5 years, 2 months ago)

Quote:







you know my answer:awedance:


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InvisibleComebackKid
Multispore Enthusiast
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Registered: 05/27/16
Posts: 3,951
Loc: ked in the trunk of a car
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Re: Cultivation General Discussion [Re: mushboy] * 1
    #25638884 - 11/25/18 06:43 PM (5 years, 2 months ago)

It's hard to say with certainty what's going on in that plate. It doesnt look like traditional cube myc growth but it does have some of the traits near the center and around the leading edge.

Its definitely too sketchy for grain at this point but see what happens after a transfer or try whipping up a batch of plates using a different agar recipe and see how it responds on that.


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:amanita2: Substrate surface conditions / Monotub prep and care :sporedrop:

Look around you... Everything you see exists inside the mind.
Consciousness, the awareness that is experiencing this mind,
is peering in from outside the universe.

Our individual experiences are all part of the universe's experience of itself


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OfflineCzM8
Kung Foo Grip
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Registered: 05/25/18
Posts: 773
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Re: Cultivation General Discussion [Re: ComebackKid]
    #25638896 - 11/25/18 06:51 PM (5 years, 2 months ago)

Try and get another tissue sample. Personally I'd rather not try and clean that up.


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OfflineMycologist217
Frank's Disciple
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Registered: 02/13/13
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Re: Cultivation General Discussion [Re: CzM8]
    #25638907 - 11/25/18 07:01 PM (5 years, 2 months ago)

I never fruit my monotubs at spawning...I know a lot of people here do it...but in my experience the 4 or 5 days colonizing before casing and introducing to fruiting conditions drastically increases bioefficiency


--------------------
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Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.


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Invisiblevan hattonFacebookDiscord
Still a noob
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Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Re: Cultivation General Discussion [Re: Mycologist217]
    #25638940 - 11/25/18 07:19 PM (5 years, 2 months ago)

Any kind of record of that?


--------------------
If I ever give out misinformation please inform me so I can have the correct information. :cheers:

Tmethyl said:
Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy.

Caps McGee said:
:thumbsup:
Fun part is figuring out what works best for you


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InvisiblebodhisattaMDiscordReddit
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Re: Cultivation General Discussion [Re: ComebackKid]
    #25638959 - 11/25/18 07:26 PM (5 years, 2 months ago)

Quote:

ComebackKid said:
It's hard to say with certainty what's going on in that plate. It doesnt look like traditional cube myc growth but it does have some of the traits near the center and around the leading edge.

Its definitely too sketchy for grain at this point but see what happens after a transfer or try whipping up a batch of plates using a different agar recipe and see how it responds on that.



I love seeing that tag on your account
:rockon:


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