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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 9 days
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Re: Cultivation General Discussion [Re: Brain Bulb]
#25628767 - 11/20/18 09:06 PM (5 years, 2 months ago) |
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Lecanicillium
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Brain Bulb
Insane in the Membrane



Registered: 11/09/17
Posts: 1,358
Last seen: 6 months, 24 days
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Re: Cultivation General Discussion [Re: cronicr]
#25628775 - 11/20/18 09:11 PM (5 years, 2 months ago) |
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So I need to get rid of it ASAP.
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Tookitooki
Mycological Fabricator


Registered: 07/28/16
Posts: 1,157
Loc: Nowhere
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Re: Cultivation General Discussion [Re: Brain Bulb]
#25629393 - 11/21/18 07:47 AM (5 years, 2 months ago) |
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I got some time off work for the holiday, I want to play around with some sterilized sub recipes. Is there any reason to shy away from peat moss in the mix?
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Failboat
Fuck Up

Registered: 02/01/18
Posts: 8,736
Last seen: 9 hours, 14 minutes
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Re: Cultivation General Discussion [Re: mushroomnate]
#25629408 - 11/21/18 07:56 AM (5 years, 2 months ago) |
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Quote:
mushroomnate said: So,I kinda lost my APE culture on agar. I can still get it back from something like this,right? (leftovers from a g2g master)
 Grain on agar or take from the sides?
All you need is one loose grain, knock it around and roll it out onto a plate. As long as it lands in the agar you're good. More than one grain kn the plate is not a problem.
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mushroomnate
Pstranger



Registered: 05/17/17
Posts: 3,100
Last seen: 2 days, 10 hours
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Re: Cultivation General Discussion [Re: Failboat]
#25629422 - 11/21/18 08:05 AM (5 years, 2 months ago) |
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Quote:
Quirkmeister92 said:
Quote:
mushroomnate said: So,I kinda lost my APE culture on agar. I can still get it back from something like this,right? (leftovers from a g2g master)
 Grain on agar or take from the sides?
All you need is one loose grain, knock it around and roll it out onto a plate. As long as it lands in the agar you're good. More than one grain kn the plate is not a problem.
 I figured that's what I'd do,but was hesitant. I may try to take a rope off the wall and put to agar as well,before shaking. Thanks for the reassurance.
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Failboat
Fuck Up

Registered: 02/01/18
Posts: 8,736
Last seen: 9 hours, 14 minutes
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Re: Cultivation General Discussion [Re: mushroomnate]
#25629430 - 11/21/18 08:12 AM (5 years, 2 months ago) |
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Don't forget your scalpel if you wanna keep the jar away from the plate.
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Brain Bulb
Insane in the Membrane



Registered: 11/09/17
Posts: 1,358
Last seen: 6 months, 24 days
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Re: Cultivation General Discussion [Re: Failboat]
#25630168 - 11/21/18 02:22 PM (5 years, 2 months ago) |
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Clones of the Cambodians are coming along!

Also inoculated some plates with Pc. Brazil a couple days ago that are coming along nicely! Used sterile swabs to inoculate this time and think I prefer them to a loop..
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Mycologist217
Frank's Disciple



Registered: 02/13/13
Posts: 2,425
Loc: Man of the Moon
Last seen: 1 day, 7 hours
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Re: Cultivation General Discussion [Re: Tookitooki]
#25630227 - 11/21/18 02:38 PM (5 years, 2 months ago) |
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Quote:
Tookitooki said: I got some time off work for the holiday, I want to play around with some sterilized sub recipes. Is there any reason to shy away from peat moss in the mix?
Most substrate get pasteurized rather than sterilized. The purpose of substrate is mainly water retention, with some supplemental nutrition.
If you want a substrate that does well when sterilized hardwood fuel pellets supplemented with bran and organic chicken shit is a really great option.
As for the Lime...it is a ph regulator that serves best as a casing component rather than substrate.
The Nitrogen provided by organic chicken poo is a much better option in substrate than Lime.
Past that lime needs to be exactly properly pastwurized or you will have a mold fest on your hands
Have fun experimenting.
Also look knto Sloppy Josephs Bottle tek substrate recipe.
-------------------- My LC Manual (With custom LC lid Tek) ~~ Required Mycology Supplies ~~ Agar Work Videos ~~ L G M AMU Q&A-NO SYMPATHY FOR THE DEVIL! KEEP THAT IN MIND! BUY THE TICKET: TAKE THE RIDE Check out my Retail Gourmet Mushroom Farm! Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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cronicr is that you?
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Brain Bulb
Insane in the Membrane



Registered: 11/09/17
Posts: 1,358
Last seen: 6 months, 24 days
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Re: Cultivation General Discussion [Re: eatyualive]
#25631289 - 11/21/18 11:27 PM (5 years, 2 months ago) |
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Hey guys. Only my second close attempt so wanted to ask if this is normal. It happened on one of my Caerulescens clones as well, but curious what it is.
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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Cultivation General Discussion [Re: Brain Bulb]
#25631612 - 11/22/18 05:51 AM (5 years, 2 months ago) |
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Bacteria
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Cultivation General Discussion [Re: mushboy]
#25631736 - 11/22/18 07:21 AM (5 years, 2 months ago) |
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Lignicolous Cubensis (which can be seen fruiting from "enriched" soils and composts), images:
T-Gel, test recipe (minimum sugars, sucrose, additional macro-micro nutrients):

Spent tea bag (minimum sugars, sucrose, spent until roughly pH 6.5):

In both cases the spores where germinated on WL-Tek, using a wooden peg with minimum sugars. The pegs where later transferred to a lignicolous material, in this case black tea leaves.
Cubensis produces enzymes to decay cellulose, proteins, and phenols.
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Inhibitory effects and lower pH's (acidic) effect general growth speed negatively. Good growth was observed when additional nitrogen was added.
Tea leaves contain up to 3% nitrogen, none added.
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Illustrations of what type of visible growth to look for:



Can be bushy when pushing up, or when populated.


Yes it's growing on CaCO3 rocks.
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Tookitooki
Mycological Fabricator


Registered: 07/28/16
Posts: 1,157
Loc: Nowhere
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Quote:
Most substrate get pasteurized rather than sterilized. The purpose of substrate is mainly water retention, with some supplemental nutrition.
If you want a substrate that does well when sterilized hardwood fuel pellets supplemented with bran and organic chicken shit is a really great option.
As for the Lime...it is a ph regulator that serves best as a casing component rather than substrate.
The Nitrogen provided by organic chicken poo is a much better option in substrate than Lime.
Past that lime needs to be exactly properly pastwurized or you will have a mold fest on your hands
Have fun experimenting.
Also look knto Sloppy Josephs Bottle tek substrate recipe.
Peat moss has a greater retention of water then coir does, it is a lot more acidic tho. The goal of the peat is to see if I can get my PE to fruit without a 50/50 casing. By putting it in the sub. My goal is to get back to the manure and straw based subs that used to rule this land. I'm going to be sterlizing instead of pasteurizing tho. Inspired by azur sterlized poo testing he did.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Cultivation General Discussion [Re: Tookitooki]
#25631797 - 11/22/18 07:58 AM (5 years, 2 months ago) |
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https://homeguides.sfgate.com/peat-moss-vs-coconut-fiber-69786.html https://www.planetnatural.com/peat-vs-coir/

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When I used 50/50 compost with peat for testing, I added lime to increase the pH. I got best growth around pH 7-7.5, I have not tried peat enriched.
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The general rule would be to check the nutritional value (% per 100g) and pH. Anything that is carbon rich, and can hold lot's of water, is ideal.
You would then modify the media to suit, and test it.
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JHOVA
Post whore


Registered: 02/17/17
Posts: 4,727
Loc:
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Re: Cultivation General Discussion [Re: Tookitooki]
#25632151 - 11/22/18 10:02 AM (5 years, 2 months ago) |
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Verum subsequentis and psycho delicacy are the pe bag people to ask
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π΄ π° πΌ π² π» πΈ π½ πΆ π
π
π° πΏ
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Tookitooki
Mycological Fabricator


Registered: 07/28/16
Posts: 1,157
Loc: Nowhere
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Re: Cultivation General Discussion [Re: Ferather]
#25632194 - 11/22/18 10:14 AM (5 years, 2 months ago) |
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I'm going to put a few mixes together in jars, going to run them thru a PC cycle, then test the pH to see what adjustments need to be made and to record the pH.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Cultivation General Discussion [Re: Tookitooki]
#25632292 - 11/22/18 11:04 AM (5 years, 2 months ago) |
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After doing some Googling, the pH and phenol content of peat moss will be similar to black tea (but without the rich macro-micro nutrients). Peat moss is high in tannins, and therefor can be decayed by laccases and peroxidases, but will have a low pH (close to tea).
My guess is that about 33%+ of the composition is phenols, mostly tannic acids. Tannic acid will react with CaCO3. The reaction produces calcium-tannate (insoluble), which ultimately neutralizes the tannic acid.
I would suggest a CaCO3 bath, and then straining if possible, then mix to enrich.
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Edit: Don't use gypsum (calcium-sulphate), use CaCO3 (lime).
Good luck.
Edited by Ferather (11/22/18 11:30 AM)
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thelanzii

Registered: 11/13/12
Posts: 5,434
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Re: Cultivation General Discussion [Re: Ferather]
#25632976 - 11/22/18 05:41 PM (5 years, 2 months ago) |
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I have 30 jars I made on Sunday and Monday of this past week. Won't be home until Sunday. Will they still be good to inocukate? Most I have ever waited is like 2 days to inoculate
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Brain Bulb
Insane in the Membrane



Registered: 11/09/17
Posts: 1,358
Last seen: 6 months, 24 days
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Re: Cultivation General Discussion [Re: Brain Bulb]
#25633002 - 11/22/18 06:00 PM (5 years, 2 months ago) |
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Can anyone offer some advice on this? Donβt want to throw the plate away if this is something that happens with clones. Only my second attempt so I was wondering if this is normal. It happened on one of my Caerulescens clones as well, but curious what it is.
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Guy on a buffalo
Apprentice Cultivator


Registered: 10/30/18
Posts: 351
Loc: Ridin around through the ...
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Re: Cultivation General Discussion [Re: thelanzii]
#25633004 - 11/22/18 06:00 PM (5 years, 2 months ago) |
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Quote:
EtherealMind said: I have 30 jars I made on Sunday and Monday of this past week. Won't be home until Sunday. Will they still be good to inocukate? Most I have ever waited is like 2 days to inoculate
Yeah they'll be fine
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