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Invisiblevan hattonFacebookDiscord
Still a noob
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Registered: 11/23/14
Posts: 5,617
Loc: Michigan
Trusted Cultivator
Re: 2 ?'s Aborts & Dehydration [Re: van hatton]
    #25599181 - 11/07/18 05:00 PM (5 years, 2 months ago)

What's on the bottom of your tub?

Can someone change the title idr what thread I'm in.


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If I ever give out misinformation please inform me so I can have the correct information. :cheers:

Tmethyl said:
Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy.

Caps McGee said:
:thumbsup:
Fun part is figuring out what works best for you


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Offlinebutterflyaway
Kaleidoscope Queen
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Registered: 10/26/18
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Re: 2 ?'s Aborts & Dehydration [Re: van hatton]
    #25599212 - 11/07/18 05:10 PM (5 years, 2 months ago)

:thanx:


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There is a better way.  Finding the way — well that's the journey:mushroom2:


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OfflineDreamReality
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Registered: 03/31/09
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Last seen: 3 months, 13 days
Re: Cultivation General Discussion [Re: Ferather] * 1
    #25599222 - 11/07/18 05:18 PM (5 years, 2 months ago)

Changed.


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OfflineBrain Bulb
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Re: Cultivation General Discussion [Re: Ferather]
    #25599603 - 11/07/18 08:51 PM (5 years, 2 months ago)

I don’t think light is the issue.  Came home from work today and the tub has erupted with pins.:-)
Maybe underwatering at some point causing the sub to pull away from the sides and leaving room for pins?  Idk.

Here a pic from a few minutes ago.  Can see a lot more pins just under the surface.  Very skinny species or maybe just a bad phenotype.


Another question.  On my other tub it looks like some of the caps are collapsing in.  Too much water, not enough air??


Thanks in advance!

BB


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Offlinetryptkaloids
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Re: Cultivation General Discussion [Re: Brain Bulb] * 1
    #25599763 - 11/07/18 10:44 PM (5 years, 2 months ago)

Thats too much water


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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OfflineBrain Bulb
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Re: Cultivation General Discussion [Re: tryptkaloids]
    #25599820 - 11/07/18 11:19 PM (5 years, 2 months ago)

Quote:

tryptkaloids said:
Thats too much water




One or both?


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InvisibleNimph
Im Unclecaptainblue!!!!

Registered: 08/12/18
Posts: 1,605
Loc: The dirty
Re: 2 ?'s Aborts & Dehydration [Re: butterflyaway]
    #25599831 - 11/07/18 11:30 PM (5 years, 2 months ago)

Put em in a freezer bag, keep air and water aaawwwaaaaay from them.

The longer u wait to dry the longer it'll take to dry em.


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Hunters save your exotic prints to trade! https://www.shroomery.org/forums/showflat.php/Number/25617539:shroomeryhead:


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Offlinetryptkaloids
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Re: Cultivation General Discussion [Re: Brain Bulb]
    #25599841 - 11/07/18 11:39 PM (5 years, 2 months ago)

Quote:

Brain Bulb said:
Quote:

tryptkaloids said:
Thats too much water




One or both?



The second one sorry


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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OfflineIntergalacticSpore
Grows shrooms on agar
Male


Registered: 08/30/18
Posts: 194
Loc: Eastern Europe
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Re: Cultivation General Discussion [Re: tryptkaloids]
    #25600429 - 11/08/18 09:17 AM (5 years, 2 months ago)

If you get spores out of culture that is weak does that mean it can go back to being strong? Or do you always need to go back to strong master culture?


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One day I will evolve from agar mushroom grower :goat:


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Offlinetryptkaloids
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Re: Cultivation General Discussion [Re: IntergalacticSpore]
    #25600576 - 11/08/18 10:11 AM (5 years, 2 months ago)

Spores are a reset.


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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OfflineFishLevelMidnight
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Registered: 09/01/17
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Re: Cultivation General Discussion [Re: tryptkaloids]
    #25600656 - 11/08/18 10:40 AM (5 years, 2 months ago)

Is that true of a monoculture?

I need to check the genetics of mushrooms but if you have a single set of genetics, and they produce spores, how is variance introduced into the spores? They have no different genetics to cross over with.

anyone?


--------------------





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Offlinetryptkaloids
Learner
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Re: Cultivation General Discussion [Re: FishLevelMidnight]
    #25600663 - 11/08/18 10:42 AM (5 years, 2 months ago)

That's a crazy good question. The only person I think would know is either verum or workman


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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OfflineG0OD
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Registered: 08/13/18
Posts: 20
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Re: Cultivation General Discussion [Re: tryptkaloids]
    #25600911 - 11/08/18 12:43 PM (5 years, 2 months ago)



are these fellas ok to eat? looking a lil different all over. pos too much water by the sounds of it


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Offlineteladi
FUNKSOULBROTHER


Registered: 06/27/17
Posts: 1,189
Loc: South Africa Flag
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Re: Cultivation General Discussion [Re: FishLevelMidnight]
    #25600989 - 11/08/18 01:10 PM (5 years, 2 months ago)

Quote:

fishermansjc said:
Is that true of a monoculture?

I need to check the genetics of mushrooms but if you have a single set of genetics, and they produce spores, how is variance introduced into the spores? They have no different genetics to cross over with.

anyone?




Yes.

A monoculture is dikaryotic. i.e. each normal cell has two sets nuclei. When a spore is formed, genetic material is exchanged between these.

If you could bring together two genetically identical spores, then you will end up with a monoculture that produces clones of itself, with some changes due to random mutations.

Quote:


Meiosis, often called "reduction division", yields four daughter nuclei with the number of chromosomes reduced to half the original number. The overall result is that one diploid nucleus becomes four haploid nuclei. Although each daughter nucleus contains a full set of chromosomes, events during meiosis ensure that each of these nuclei contains genetic material derived from both parents and that each daughter nucleus is genetically different from the others.





http://website.nbm-mnb.ca/mycologywebpages/NaturalHistoryOfFungi/ReproductionDiscussion.html

Some types of fungi can reproduce asexually. In these, the parent produces spores genetically identical to itself, throws them into the wind, and when they land the germinate into a clone of the parent.


Edited by teladi (11/08/18 01:22 PM)


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OfflineIntergalacticSpore
Grows shrooms on agar
Male


Registered: 08/30/18
Posts: 194
Loc: Eastern Europe
Last seen: 2 years, 11 months
Re: Cultivation General Discussion [Re: teladi]
    #25601024 - 11/08/18 01:22 PM (5 years, 2 months ago)

Is there a way to determine if your mushrooms are a monoculture? How do they act compared to the ones that aren't mono? Is there a good book on this topic, I'm starting to feel like I'm annoying everyone with my weird questions.


--------------------
One day I will evolve from agar mushroom grower :goat:


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Offlinegizmodo
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Re: Cultivation General Discussion [Re: IntergalacticSpore]
    #25601430 - 11/08/18 04:55 PM (5 years, 2 months ago)

Has anyone here picked up a delivery from an AzureStandard drop location before?
I just placed an order for 50lbs of rye grain and I'm wondering how it works.
Seems like a truck drops off and I need to be there at a certain time.


--------------------

One must be mindful to maintain their balance on the slippery earth.

Bod's Comprehensive Agar Resource
Gizmodo's Market Stall
Say No To Grow Kits


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Offlineteladi
FUNKSOULBROTHER


Registered: 06/27/17
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Loc: South Africa Flag
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Re: Cultivation General Discussion [Re: IntergalacticSpore] * 1
    #25602083 - 11/08/18 11:47 PM (5 years, 2 months ago)

Quote:

IntergalacticSpore said:
Is there a way to determine if your mushrooms are a monoculture? How do they act compared to the ones that aren't mono?




I was hoping a TC would weigh in there.

I believe an experienced eye would be able to tell a mono apart from a MS culture. Monos should show more consistent and uniform growth.

Scientifically, you should be able to devise a test. Take samples, extract DNA, cut it up - with cuts made at very specific sequences - amplify the fragments, and separate them through gel electrophoresis. You'll end up with a barcode type pattern that you see on movies.



Depending on how well you selected your targets, those barcodes may be good enough to distinguish between individuals.


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OfflineRomeoPapa
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Re: 2 ?'s Aborts & Dehydration [Re: teladi] * 1
    #25602108 - 11/09/18 12:23 AM (5 years, 2 months ago)

:breakthrough: 

And all I wanted to do was grow mushrooms!


--------------------
It's better to have it and not need it
Than it is to need it and not have it.



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OfflineIntergalacticSpore
Grows shrooms on agar
Male


Registered: 08/30/18
Posts: 194
Loc: Eastern Europe
Last seen: 2 years, 11 months
Re: Cultivation General Discussion [Re: teladi]
    #25602352 - 11/09/18 06:08 AM (5 years, 2 months ago)

Quote:

teladi said:
Quote:

IntergalacticSpore said:
Is there a way to determine if your mushrooms are a monoculture? How do they act compared to the ones that aren't mono?




I was hoping a TC would weigh in there.

I believe an experienced eye would be able to tell a mono apart from a MS culture. Monos should show more consistent and uniform growth.

Scientifically, you should be able to devise a test. Take samples, extract DNA, cut it up - with cuts made at very specific sequences - amplify the fragments, and separate them through gel electrophoresis. You'll end up with a barcode type pattern that you see on movies.



Depending on how well you selected your targets, those barcodes may be good enough to distinguish between individuals.



Quote:

RomeoPapa said:
:breakthrough: 

And all I wanted to do was grow mushrooms!



:canthelpbutlaugh:I love this reaction.


--------------------
One day I will evolve from agar mushroom grower :goat:


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OfflineBrain Bulb
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Registered: 11/09/17
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Re: Cultivation General Discussion [Re: IntergalacticSpore]
    #25602614 - 11/09/18 09:30 AM (5 years, 2 months ago)

This is the first tub I’ve done larger than a shoebox.  It’s 3 jars GS in a 36 qt tub.  Transferred my GS to CV sub about a week ago.  Does this look right?



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