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IntergalacticSpore
Grows shrooms on agar



Registered: 08/30/18
Posts: 194
Loc: Eastern Europe
Last seen: 2 years, 11 months
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Re: Cultivation General Discussion [Re: teladi]
#25598313 - 11/07/18 11:00 AM (5 years, 2 months ago) |
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So I was just pouring the agar into my plastic petris which I finally bought, kinda had to I can't really own more glass ones.
While I was pouring the agar a titty implant sized thing fell out and landed in petri dish much to my amusement. It luckily didn't splash and I could still close it.
I know I should pour agar at 50C, I poured it at 43 (had thermometer glued to the side of the bottle).
So my question is at what temperature does agar solidify? I was using 1.5% agar 2% dme mix.
--Edit: Oh wow I'm the guy that opened 1000th page of this thread
-------------------- One day I will evolve from agar mushroom grower
Edited by IntergalacticSpore (11/07/18 11:01 AM)
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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@Mateah
It's not common information, you can find some here in my pocket guide.
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Acidification (Simple edition):
Acidification can cause mycelium to fruit (various reasons), allowing for mild acidification and then increasing the pH, increases the number of cycles.
Once the media pH reaches a level the mycelium and-or it's enzymes are no longer stable, it will not produce any more fruits. At this point the media pH must be increase (reset to original), any waste potential should also be removed.
This is where 'some' secondary decomposers would come in, tolerant to the acidification (acid stable enzymes and low pH tolerance). The dung which cubensis likes, is not acidic, it has a pH of 6.5+, so some decomposers come in when the pH has increased.
As acidification takes place, and enzymes begin to destabilize, mycelium will start to respond to low(er) nutrients or low(er) uptake. Usually there a few different types of enzyme that target a material, the main setting difference is the stable pH range.
Acidification can also effect various protease enzymes, but not other enzymes (causing a low nitrogen uptake).
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If you dunk with CaCO3 you help this process and also allow the mycelium to remove oxalates. Under optimal conditions, fruiting potential will likely be capped by nutrients.
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Edit: Some extra data here: https://www.shroomery.org/forums/showflat.php/Number/25593211#25593211
Edited by Ferather (11/07/18 01:54 PM)
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Brain Bulb
Insane in the Membrane



Registered: 11/09/17
Posts: 1,358
Last seen: 6 months, 24 days
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Re: Cultivation General Discussion [Re: Ferather]
#25598865 - 11/07/18 02:52 PM (5 years, 2 months ago) |
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What do I need to do to minimize side pins on future grows? The two boxes that are fruiting now have a lot. Not sure what I did wrong.

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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Cultivation General Discussion [Re: Brain Bulb]
#25598941 - 11/07/18 03:31 PM (5 years, 2 months ago) |
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That can happen if the spawn is running out of nutrients (various reasons), causing it to fruit in those area's. It can also happen due to light, and if the mycelium has no direction (no idea where is up).
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The following data will vary slightly depending on the mycelium being grown.
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Low nutrient (the paper spawn is turning into compost), old recipe:


Note: seaweed should be avoided.
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Surface detection (higher O2, notice the surface growth):

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Lid left on, pinning in the area with highest O2:

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mushboy
modboy



Registered: 04/24/05
Posts: 32,281
Loc: where?
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Re: Cultivation General Discussion [Re: Ferather]
#25598948 - 11/07/18 03:34 PM (5 years, 2 months ago) |
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Quote:
Ferather said: That can happen if the spawn is running out of nutrients (various reasons), causing it to fruit in those area's. It can also happen due to light, and if the mycelium has no direction (no idea where is up).
thats not what causes side pins. its all about conditions near the tote and the substrate being more ideal for pins than the surface.
side pins are not caused by light, not knowing up vs down, or lack of nutes. never heard lack of nutes being a cause before.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Cultivation General Discussion [Re: mushboy]
#25598959 - 11/07/18 03:39 PM (5 years, 2 months ago) |
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I mean spent, running out.
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
Last seen: 3 days, 15 hours
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Re: Cultivation General Discussion [Re: Ferather]
#25598969 - 11/07/18 03:44 PM (5 years, 2 months ago) |
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these are clearly first flush pins. if it were due to sub running out then we would see a lot more shrinkage and a lot less smashed fruit
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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Sofaking420



Registered: 02/09/18
Posts: 1,126
Loc: Myco mountain
Last seen: 1 day, 1 hour
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Re: Cultivation General Discussion [Re: Ferather]
#25598972 - 11/07/18 03:45 PM (5 years, 2 months ago) |
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What causes mutant fruits? I recently got a new print from a site sponcer and the first flush is mostly mutant fruits. Many fruits grew upside down, gnarly stems.
It is my first mono tub, although im treatinf it like a large shoebox.
Can conditions cause mutants? Eg. Bacteria, low temps, etc???
Or did i get a "bad" print?
Ive only seen one or two mutant fruits in the last year using a different sponcer
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
Last seen: 3 days, 15 hours
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Re: Cultivation General Discussion [Re: Sofaking420]
#25598981 - 11/07/18 03:49 PM (5 years, 2 months ago) |
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genetics are a lottery. certain varieties are more likely to mutate like PE or KSSS but yes bacteria can cause mutants, some mutations can be as simple as over wet sub. premature veil tearing is known to occur in said case
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Cultivation General Discussion [Re: tryptkaloids]
#25598990 - 11/07/18 03:53 PM (5 years, 2 months ago) |
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Quote:
tryptkaloids said: these are clearly first flush pins. if it were due to sub running out then we would see a lot more shrinkage and a lot less smashed fruit
I used spent media to spawn that test run, hence the darker colour (much older, more decayed). There is also seaweed bacteria, which doesn't compete, but does decay and feed.
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Sofaking420



Registered: 02/09/18
Posts: 1,126
Loc: Myco mountain
Last seen: 1 day, 1 hour
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Re: Cultivation General Discussion [Re: tryptkaloids]
#25598993 - 11/07/18 03:53 PM (5 years, 2 months ago) |
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I know pics help. It was cambodian that i ordered
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Cultivation General Discussion [Re: Sofaking420]
#25599008 - 11/07/18 03:57 PM (5 years, 2 months ago) |
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Looks dry to me, probably to much dry mass in the fruit bodies. If not maybe bacteria? It also looks like there isn't enough FAE, from what I can see.
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
Last seen: 3 days, 15 hours
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Re: Cultivation General Discussion [Re: Sofaking420]
#25599017 - 11/07/18 03:59 PM (5 years, 2 months ago) |
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Quote:
Sofaking420 said: I know pics help. It was cambodian that i ordered

bacteria and over wet sub.
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
Last seen: 3 days, 15 hours
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Re: Cultivation General Discussion [Re: tryptkaloids]
#25599020 - 11/07/18 04:00 PM (5 years, 2 months ago) |
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you can tell by the splotchy caps and the one in the middle back being all wavy that early
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: Cultivation General Discussion [Re: tryptkaloids]
#25599036 - 11/07/18 04:05 PM (5 years, 2 months ago) |
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I'm curious about the setup, what is the humidity and how are you getting FAE (is the lid off, a device)?
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butterflyaway
Kaleidoscope Queen



Registered: 10/26/18
Posts: 114
Loc:
Last seen: 3 years, 5 months
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2 ?'s Aborts & Dehydration [Re: Ferather]
#25599098 - 11/07/18 04:27 PM (5 years, 2 months ago) |
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On my second flush day 3. Wondering if these pins are aborts?
Also, I got a dehydrator, had 65g fresh (they’d been paper bagged in the fridge a couple of days) and feel like I let them dry out too much cause now they are 4g. I’ve read it doesn’t really effect potency but... what about dosage?
-------------------- There is a better way. Finding the way — well that's the journey
Edited by butterflyaway (11/07/18 04:30 PM)
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DreamReality
MycoPilot



Registered: 03/31/09
Posts: 477
Last seen: 3 months, 13 days
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Quote:
butterflyaway said: On my second flush day 3. Wondering if these pins are aborts?
Also, I got a dehydrator, had 65g fresh (they’d been paper bagged in the fridge a couple of days) and feel like I let them dry out too much cause now they are 4g. I’ve read it doesn’t really effect potency but... what about dosage?

I'd eat all 4g. That's pretty much a good starter level trip
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,641
Loc: Exact Center
Last seen: 3 days, 15 hours
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Those are not aborts
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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butterflyaway
Kaleidoscope Queen



Registered: 10/26/18
Posts: 114
Loc:
Last seen: 3 years, 5 months
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Quote:
DreamReality said:
Quote:
butterflyaway said: On my second flush day 3. Wondering if these pins are aborts?
Also, I got a dehydrator, had 65g fresh (they’d been paper bagged in the fridge a couple of days) and feel like I let them dry out too much cause now they are 4g. I’ve read it doesn’t really effect potency but... what about dosage?

I'd eat all 4g. That's pretty much a good starter level trip
Cool
Any opinion on the pics? Aborts? Still maturing? Wait n see? Make some tea? Ha! so many options...
-------------------- There is a better way. Finding the way — well that's the journey
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van hatton
Still a noob


Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Those are not aborts.
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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