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Re: Cultivation General Discussion [Re: Mad Season] * 2
    #23528298 - 08/10/16 01:51 PM (7 years, 5 months ago)

Quote:

Mad Season said:
IMO they're worth it. They also have sclerotia lol. One of the only pans which form sclerotia.

Does anyone have proof that their substrate bruised from handling and direct misting rather than dry conditions and/or contaminations? Been looking for someone who has experience with handling bruising on a sub, and it wasn't because of conditions. Personally I haven't ever seen any bruising from anything but dry conditions, yet every noob says you can get bruising from handling.



To be honest now that I think about it I've never bruised a bulk sub even after dropping it or breaking it in half accidentally.

They never blue like cakes did for me


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Re: Cultivation General Discussion [Re: tombosley8] * 1
    #23530037 - 08/11/16 01:14 AM (7 years, 5 months ago)



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Re: Cultivation General Discussion [Re: blindingleaf]
    #23617234 - 09/06/16 07:17 AM (7 years, 4 months ago)

170F kills trich spores in a second too


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Re: Cultivation General Discussion [Re: tombosley8]
    #23617805 - 09/06/16 11:09 AM (7 years, 4 months ago)

Sterilization of your tools is a good idea. But not necessary. You'll flame them before use but getting the tool a full PC cycle now and then is not a bad idea. I use a propane torch I prefer not to dick around with butane torches even good ones that cost 70$ break in a few years a brass propane tip lasts forever(without the piezoelectric shit)

When working at the sab i run the torch on low, I have a little baby food jar of 91% to Dip my scalpel or inoculation loop in before flaming


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Re: Cultivation General Discussion [Re: Kenetic]
    #23618113 - 09/06/16 12:49 PM (7 years, 4 months ago)

Alcohol lamps I despise not hot enough imo and a nearly clear flame. Propane is way less expensive as well.


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Re: Cultivation General Discussion [Re: Kenetic]
    #23618146 - 09/06/16 12:58 PM (7 years, 4 months ago)

Yes that's completely insufficient


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Re: Cultivation General Discussion [Re: BrutusIsBig]
    #23635080 - 09/11/16 09:20 AM (7 years, 4 months ago)

Both q&a forums are in my signature


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Re: Cultivation General Discussion [Re: Musheadfred]
    #23637114 - 09/11/16 08:41 PM (7 years, 4 months ago)

clean up so you don't get bugs


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Re: Cultivation General Discussion [Re: filthyknees]
    #23656291 - 09/18/16 04:55 PM (7 years, 4 months ago)

the PC has never hurt my syringes. time has.

I use the boiling water method all the time, but if I'm doing a run and want a syringe I throw it in. it gets 2 hours at 18psi and comes out just fine.


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Re: Cultivation General Discussion [Re: Munchauzen]
    #23683907 - 09/27/16 05:34 PM (7 years, 3 months ago)

Stop trich don't try to inhibit it, I would use CVG as is


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Re: Cultivation General Discussion [Re: Terpfreak]
    #23712723 - 10/06/16 10:20 AM (7 years, 3 months ago)

Agar all you need last link in my signature


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Re: Cultivation General Discussion [Re: Terpfreak]
    #23712831 - 10/06/16 11:06 AM (7 years, 3 months ago)

Quote:

Terpfreak said:
Im starting with plastic type medical grade dishes, what sterilization process would I use?



They come sterilized. They melt if you sterilize them yourself. They're gamma sterilized when made.


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Re: Cultivation General Discussion [Re: morty422]
    #23721875 - 10/09/16 11:44 AM (7 years, 3 months ago)

20 plates per go is a good amount for me.

Spores to 4-5 plates. The rest to clean up


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Re: Cultivation General Discussion [Re: Moabfighter]
    #23740651 - 10/15/16 06:58 PM (7 years, 3 months ago)

You could pick one up off the floor and put it on agar. The mycelium should grow out right away, make a transfer


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Re: Cultivation General Discussion [Re: Terpfreak]
    #23740849 - 10/15/16 09:06 PM (7 years, 3 months ago)

:hi:


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Re: Cultivation General Discussion [Re: Inocuole] * 1
    #23753007 - 10/19/16 07:42 PM (7 years, 3 months ago)

Does it have merit because it doesn't not work? Frank correlated a few results with that one variable. He may have changed his tone by now has he stayed around. Maybe not. You have to make those determinations yourself


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Re: Cultivation General Discussion [Re: JacobStorm]
    #23754568 - 10/20/16 11:25 AM (7 years, 3 months ago)

Spores are quite larger than bacteria you might only really need to go to step 3-4


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Re: Cultivation General Discussion [Re: Terpfreak]
    #23754696 - 10/20/16 12:07 PM (7 years, 3 months ago)

I feel like some teacher or boss had a kid in lab that just didn't fucking get it then had to make this gif to try to drill it into their head how to get it fucking right


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Re: Cultivation General Discussion [Re: c10h12n2o]
    #23756499 - 10/20/16 11:14 PM (7 years, 3 months ago)

it's like the current year


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Re: Cultivation General Discussion [Re: Greg]
    #23767015 - 10/24/16 02:28 PM (7 years, 3 months ago)

you need to run some sort of heater. preferably radiant rather than fan forced


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