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DeadPhan



Registered: 05/05/04
Posts: 5,260
Last seen: 4 years, 2 months
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Agar dishes that never germinated?
#23526892 - 08/10/16 02:02 AM (7 years, 5 months ago) |
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I follow the basic Malt agar recipe. Which has worked in the past. I went from syringe to agar with multiple syringes and a bunch of them just never germinated. However they did on grain. Wtf.
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Big Gulps! Alright! Well, See ya later! And if i claim to be a wise man, well, it surely means that i dont know!
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Agar dishes that never germinated? [Re: DeadPhan]
#23526904 - 08/10/16 02:09 AM (7 years, 5 months ago) |
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That can happen. Try making the agar softer or including more nutrient, or both. I heard that BRF paste shit is actually pretty good for germing spores.
Or germinate it on grain, then drop a grain on agar and tidy it up.
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DeadPhan



Registered: 05/05/04
Posts: 5,260
Last seen: 4 years, 2 months
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Re: Agar dishes that never germinated? [Re: Inocuole]
#23526910 - 08/10/16 02:12 AM (7 years, 5 months ago) |
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Word. That's what I'll have to do. I have jars that I didn't shake up after g2g that have cobweb but the cobweb is below the healthy myc on top. I'll just do that and tidy up. Tha KS. I was spacing on that notion.
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Big Gulps! Alright! Well, See ya later! And if i claim to be a wise man, well, it surely means that i dont know!
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sandy_vag
Keep Dreaming


Registered: 06/14/16
Posts: 112
Last seen: 5 years, 8 months
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Re: Agar dishes that never germinated? [Re: DeadPhan]
#23528419 - 08/10/16 02:35 PM (7 years, 5 months ago) |
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I've never been able to get a plate to colonize with a syringe, only from a print. I usually do the grain to plate method. Popcorn is good for this, seems to take off faster.
Edited by sandy_vag (08/10/16 02:36 PM)
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Agar dishes that never germinated? [Re: sandy_vag]
#23528437 - 08/10/16 02:41 PM (7 years, 5 months ago) |
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Doing this dramatically helps your chances as well as softer agar
Quote:
Trusted Cultivator said:
Quote:
Trusted Cultivator said:
Quote:
bodhisatta said:
Quote:
bodhisatta said:
take your spores to an inoculation loop. if you have a print then flame the loop dip in agar, pick spores off print and then swab the dish
if you have a syringe flame the loop and drop a few drops onto the loop to cool it down and then one more drop to load it with spores and then swab that


Quote:
bodhisatta said: Try using an inoculation loop. Flame the syringe needle and the loop then squirt onto the loop to cool the needle and the loop. Squirt another drop to prime the loop. Then swab the dish.

 
Then you can see growth immediately, see contamination easier, grab healthy mycelium easier, and never worry about spore drops rolling all over a dish giving you germination everywhere at the same time.
also the agar link in my signature
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Psychedel.EXE
AKA Old Uncle Nutty



Registered: 07/04/16
Posts: 211
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Re: Agar dishes that never germinated? [Re: sandy_vag]
#23528444 - 08/10/16 02:43 PM (7 years, 5 months ago) |
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When I have done it, I would just make like 8 or so plates and use a syringe to drop ONE drop carefully as central as possible on each pastyplate. Chances are there are a few plates that look less than desirable, and maybe a few that didn't germinate. The 2 best looking plates made it to round 2 where I do 8 fresh transfers from each of my 2 chosen plates. Now I have 16 plates to choose from. And if necessary I can keep going until I am drowning in pastyplates. I love having this problem.
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