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Kurze
Night Haunter



Registered: 07/05/14
Posts: 13
Last seen: 1 year, 2 days
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first time G2G question
#23526282 - 08/09/16 09:53 PM (7 years, 5 months ago) |
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So I watched some videos, and was reading some teks and had a question about G2G. Most of the videos involved a flow hood, which I don't have. In the videos they flip the lids upside down and place them on top of another jar while transferring grains.
My understanding is that when they flip it back over, the flow hood would wipe off any bacteria on the lid bottom. If I'm using a sab, should I flip them upside down? Or should I stick an empty jar in my box, and place the lid right side up on top of that?
I just want to minimize risk since it's my first time doing it. I'm just not sure if one way would work better than the other. Thanks in advance for any clarification.
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Psilicon
Really Nice Guy

Registered: 08/26/12
Posts: 7,057
Last seen: 3 years, 1 month
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Re: first time G2G question [Re: Kurze]
#23526301 - 08/09/16 09:57 PM (7 years, 5 months ago) |
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When I do G2G in a SAB, I unscrew the caps but leave them on. Just pick up the lid with your other hand just before transferring.
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Kurze
Night Haunter



Registered: 07/05/14
Posts: 13
Last seen: 1 year, 2 days
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Re: first time G2G question [Re: Psilicon]
#23526314 - 08/09/16 10:01 PM (7 years, 5 months ago) |
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So when I twirl the master jar, I just keep the lid in my other hand? I would just slide it back on after pouring them right? Would only be doing 1 at a time anyways, so that would make sense. I guess with flow hoods it would be easier putting them aside instead of risking possible contams blowing off your hand or lid into either jar. Thanks for the help! I appreciate it. Next thing to try is agar. Did pastywhytes tek a year or so ago with a bit of success. My pressure cooker was crap though so I had a few issues.
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: first time G2G question [Re: Psilicon]
#23526315 - 08/09/16 10:02 PM (7 years, 5 months ago) |
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I don't ever set the lid down, bad juju.
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anthiawe
friendly stranger


Registered: 05/18/16
Posts: 652
Last seen: 2 years, 7 months
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Re: first time G2G question [Re: Inocuole]
#23527184 - 08/10/16 04:52 AM (7 years, 5 months ago) |
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i'm bad for putting lids down, but i also work with oysters most of the time and they forgive. actives are different. sterile technique, aka everything you do around culture, is important!
...incocuole is worried about juju, didn't know he was a traditionalist african. wonder what his pre-SAB rituals are?
-------------------- TEK compendium
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