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ijustwantobehuman
MastoDAAAMN

Registered: 03/03/15
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How do my jars look?
#23525481 - 08/09/16 05:20 PM (7 years, 5 months ago) |
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 Day 11 WBS. How do they look to you experts?
Backstory Last attempt I had 6 jars rye berries and 3 wbs all inoculated with Ecuador. Every single jar -GREEN MOLD! Tossed them, figured I undercut my PC time
- Now: Did 9 jars WBS PCd 3@60 min 20psi 3@80min 20psi 3@90 min 20psi
Inoculated 6 with Ecuador again and 3 with GT All Ecuador went green again.very sad, was looking forward to the Ecuador Must be a bad syringe?
These are the results though. 2cc each jar of GT My 60min jar suxxx . Very little growth, wasn't even worth a picture. The least colonised jar was the 80min jar.
Will be doing 3 jars WBS @90min 20psi this weekend with Chitwan Nepal and APE - first time for both!
Tips, pointers and current TOOL song you're listening to?
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anthiawe
friendly stranger


Registered: 05/18/16
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the three jars you have up there look fine. the general rule for grain sterilization is 90 minutes at 15psi. is all you have a 20? or can you change that?
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Marty Mycfly
Time Traveler


Registered: 12/16/13
Posts: 976
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The problem is whatever your inoculating with. If your jars were under Pc'd you would see bacteria not green mold. Maybe technique is the vector, but I'd say it's your spore syringe.
Edit, and in the picture your grains are starting to look greasy that are't colonized, that could be bacteria, if those don't colonize by tomorrow it definitely is.
Edited by Marty Mycfly (08/09/16 05:46 PM)
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anthiawe
friendly stranger


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Last seen: 2 years, 7 months
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interesting point marty, thanks for sharing. what would bacteria look like if not green mold? white? black? or the rainbow?
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ijustwantobehuman
MastoDAAAMN

Registered: 03/03/15
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Last seen: 1 year, 10 days
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Quote:
anthiawe said: the three jars you have up there look fine. the general rule for grain sterilization is 90 minutes at 15psi. is all you have a 20? or can you change that?
Yes all I have is a 20+
Quote:
Marty Mycfly said: The problem is whatever your inoculating with. If your jars were under Pc'd you would see bacteria not green mold. Maybe technique is the vector, but I'd say it's your spore syringe.
Edit, and in the picture your grains are starting to look greasy that are't colonized, that could be bacteria, if those don't colonize by tomorrow it definitely is.
What bacteria am I looking for?
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Psychedel.EXE
AKA Old Uncle Nutty



Registered: 07/04/16
Posts: 211
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Re: How do my jars look? [Re: anthiawe]
#23525538 - 08/09/16 05:50 PM (7 years, 5 months ago) |
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You have the foil on still, remove that. The foil should not be on anymore after you remove it from the PC. The myc looks really thin and almost transparent. I am not so sure of these either. For sure wait for some more opinions though. Was that using a MS syringe? 2ccs per jar is a pretty crazy amount to use from a spore syringe. Get that foil off for sure.
EDIT: If thats what day 11 looks like after using a spore syringe to inoculate, then I bet that you are growing green jars there too.
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Edited by Psychedel.EXE (08/09/16 05:52 PM)
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Marty Mycfly
Time Traveler


Registered: 12/16/13
Posts: 976
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Wet spot, it's what you see in grain jars that were not sterilized properly, in your case look at the single grains against the glass there that are not colonized, if those are healthy jars, they should be done tommorow, if it doesnt, from there it will start to look funkier and funkier and you will be able to see a gunk building up around the uncolonized grains.
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lukehighwalker710
Cloud 9 Dweller



Registered: 03/04/16
Posts: 609
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Last seen: 6 years, 22 days
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Well I'm thinking they are contaminated! My guess would be bacteria, but obviously I could be wrong. However I say that because the mycelium appears very weak and you can still see the grain well. Here's some healthy jars for comparison: Quote:
TranscendingLife said: WBS:

Rye:
Going directly from spore to grain is a big waste of your time and efforts. I would highly recommend reading into the basics of agar (I'd start with pasty's agar tek). You can use agar to isolate healthy mycelium from any contams and insure a clean culture is being put to grain.
Agar isn't any harder to use than anything else in this hobby, nor expensive; and is pretty much necessary if you expect anything more than failure or miniscule success. Then you can use it to progress from success study man. Measure twice, cut once.
Oh and 2 degrees is the jam now.
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Highwalker
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lukehighwalker710
Cloud 9 Dweller



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Re: How do my jars look? [Re: anthiawe]
#23525546 - 08/09/16 05:54 PM (7 years, 5 months ago) |
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Quote:
anthiawe said: the three jars you have up there look fine. the general rule for grain sterilization is 90 minutes at 15psi. is all you have a 20? or can you change that?
They don't look fine. 20psi isn't the issue.
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Highwalker
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Psychedel.EXE
AKA Old Uncle Nutty



Registered: 07/04/16
Posts: 211
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Quote:
lukehighwalker710 said:
Quote:
anthiawe said: the three jars you have up there look fine. the general rule for grain sterilization is 90 minutes at 15psi. is all you have a 20? or can you change that?
They don't look fine. 20psi isn't the issue.
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anthiawe
friendly stranger


Registered: 05/18/16
Posts: 652
Last seen: 2 years, 7 months
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the carpenters proverb is always good advice. easy agar recipe here -https://www.shroomery.org/forums/showflat.php/Number/1332825#1332825 been using this one for a couple weeks, usually see growth in 2 days.
...i was just addressing his experiments with PC times.
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Marty Mycfly
Time Traveler


Registered: 12/16/13
Posts: 976
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Re: How do my jars look? [Re: anthiawe]
#23525561 - 08/09/16 05:59 PM (7 years, 5 months ago) |
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Quote:
anthiawe said: interesting point marty, thanks for sharing. what would bacteria look like if not green mold? white? black? or the rainbow?
Opaque, off white, crisco!
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Marty Mycfly
Time Traveler


Registered: 12/16/13
Posts: 976
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You wanna see a bad bacterial jar, I have a few I just pulled out of the lab
Horrible


here's what they should look like, from the batch after

This happened to be a sterilizing issue, they were rye and all 18 were sterilized for 90 min. in my AA 941, I usually sterilize for 100 min. regardless but just didnt that run and every fucking jar! I had 3 jars that didnt get inoculated, they were not meant to be test jars but Im glad I had them, they went bacterial at the same time. Grain doesnt have to be sterilized for longer than 90 min usually unless your sterilizing bags, but once in a while there is a batch of grain that goes out that is out of fucking control with endospores, Stamets talks about this happening and always makes a test batch of grains before doing a big run, I just do 100 min. regardless now.
Edited by Marty Mycfly (08/09/16 06:28 PM)
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ijustwantobehuman
MastoDAAAMN

Registered: 03/03/15
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If its any consolation, these pics were taken two hrs after shaking them up a bit. These jars colonised a lot quicker than any of my others.
2cc at ABSOLUTE max. Probably closer to 1-1.5 for most, the foil on it only serving to protect my one 1/8th hole in center of the lid. Didn't want to risk any particles although they are enclosed in an incubator
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anthiawe
friendly stranger


Registered: 05/18/16
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Last seen: 2 years, 7 months
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thanks for the pics marty. hope everyone is learning through this thread. I've seen jars of my own with the milyness, always though it was more of a hydration issue in my grains, poor straining.
-------------------- TEK compendium
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Marty Mycfly
Time Traveler


Registered: 12/16/13
Posts: 976
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Quote:
ijustwantobehuman said: If its any consolation, these pics were taken two hrs after shaking them up a bit. These jars colonised a lot quicker than any of my others.
2cc at ABSOLUTE max. Probably closer to 1-1.5 for most, the foil on it only serving to protect my one 1/8th hole in center of the lid. Didn't want to risk any particles although they are enclosed in an incubator
What, 2 hours after shaking your jars? That's pretty gnarly if that's the case, however I feel you may have your timeline mixed up, when you shake any grain jar at any time even 100% it all turns invisible pretty much for at least 12 to 24 hours.
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Marty Mycfly
Time Traveler


Registered: 12/16/13
Posts: 976
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Quote:
ijustwantobehuman said: If its any consolation, these pics were taken two hrs after shaking them up a bit. These jars colonised a lot quicker than any of my others.
2cc at ABSOLUTE max. Probably closer to 1-1.5 for most, the foil on it only serving to protect my one 1/8th hole in center of the lid. Didn't want to risk any particles although they are enclosed in an incubator
You need a filter over your jar hole for GE and protecting from contams, if you just have a hole with foil over it, that's the problem right there. Look into Synthetic Filter Disc or a diy alternative here in search area, even stuffing poly fill tight in the little hole works great. But I think we can all definitively say that until you do that you won't have any spawn to work with.
Edited by Marty Mycfly (08/09/16 07:10 PM)
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lukehighwalker710
Cloud 9 Dweller



Registered: 03/04/16
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ijustwantobehuman
MastoDAAAMN

Registered: 03/03/15
Posts: 64
Last seen: 1 year, 10 days
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Quote:
Marty Mycfly said:
Quote:
ijustwantobehuman said: If its any consolation, these pics were taken two hrs after shaking them up a bit. These jars colonised a lot quicker than any of my others.
2cc at ABSOLUTE max. Probably closer to 1-1.5 for most, the foil on it only serving to protect my one 1/8th hole in center of the lid. Didn't want to risk any particles although they are enclosed in an incubator
You need a filter over your jar hole for GE and protecting from contams, if you just have a hole with foil over it, that's the problem right there. Look into Synthetic Filter Disc or a diy alternative here in search area, even stuffing poly fill tight in the little hole works great. But I think we can all definitively say that until you do that you won't have any spawn to work with.
we'll see. Only bad jars I've had yet were from the bad syringe. Still managed to get plenty of fruit
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