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OfflineStarz_PLURR
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Got the wrong lids
    #23522472 - 08/08/16 05:07 PM (7 years, 5 months ago)

So I bought some lids online, but I think I got ones meant for LC. Will they still work for rye berries in quart jars... and while side do I put the syringe to inoculate them?




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InvisiblePsychedel.EXE
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Re: Got the wrong lids [Re: Starz_PLURR]
    #23522505 - 08/08/16 05:17 PM (7 years, 5 months ago)

Yea, those are for LCs.
It really won't provide enough GE for a quart of grain.
You could remove your watman style filter and drill the hole to a 1/4in and adhere your own SFD filter. Or whatever else you could improvise for a filter, but that little watman filter would likely cause a jar to stall before colonization.
The blue rubber piece is of course the self healing injection port for needles.

Also, keep in mind that going from spore syringe straight to grain is not ideal.

Ditch the rubber seal. Trust me. They are not necessary. I use the same style lids and I have 2 dozen rubber gaskets that never get used just taking up room in my supplies closet.


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OfflineStarz_PLURR
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Re: Got the wrong lids [Re: Psychedel.EXE]
    #23522538 - 08/08/16 05:26 PM (7 years, 5 months ago)

I got ones for grains too. I justill haven't ever went and learned LC, and I've been going from spores to grains with a SAB.


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InvisiblebodhisattaMDiscordReddit
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Re: Got the wrong lids [Re: Psychedel.EXE]
    #23522542 - 08/08/16 05:26 PM (7 years, 5 months ago)

they'll work fine for grain.

the syringe goes through the injection port. the filter is well a filter.


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InvisiblePsychedel.EXE
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Re: Got the wrong lids [Re: bodhisatta]
    #23522576 - 08/08/16 05:36 PM (7 years, 5 months ago)

I know I've seen TC's say they are no good for grain...

Quote:

TranscendingLife said:
:whatdidyoudo:

How are those Whatmans sealed to the jar lids?
Also, Whatman syringe filters are not suitable for grains.
They don't allow enough GE.




Quote:

FooMan said:
Syringe filters should be used for liquid cultures, not grain jars. They don't provide enough gas exchange for grains, whereas LC's don't need GE. Use the discs for grain jars.




Quote:

shamanamba said:
I had a lengthy reply written for this but the thread disappeared when I tried to post it.

Anyway, I'm surprised that a site sponsor is selling grain jars with syringe filters for GE. They should know that those filters are notorious for restricting GE too much and causing stalled growth.

Those filters are good for LC's and air displacement on sterile water containers, but not grain jars.





Thats what I've seen repeated a lot. I don't have my own experience to speak from.


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Edited by Psychedel.EXE (08/08/16 05:38 PM)


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InvisiblebodhisattaMDiscordReddit
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Re: Got the wrong lids [Re: Psychedel.EXE]
    #23522605 - 08/08/16 05:44 PM (7 years, 5 months ago)

it's true you've heard it. it's also been done before. i'm sure they're far from ideal


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Invisibleazur
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Re: Got the wrong lids [Re: bodhisatta]
    #23522716 - 08/08/16 06:17 PM (7 years, 5 months ago)

Quote:

Trusted Cultivator said:
they'll work fine for grain.

the syringe goes through the injection port. the filter is well a filter.



Quote:

Trusted Cultivator said:
it's true you've heard it. it's also been done before. i'm sure they're far from ideal



:backpedaling:


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A cube is NOT a cube.

FALL IN LOVE WITH LC
FOTTSE!!!
ALL NOOBS READ THIS!!!



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InvisiblebodhisattaMDiscordReddit
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Re: Got the wrong lids [Re: azur]
    #23522769 - 08/08/16 06:33 PM (7 years, 5 months ago)

hamloaf always said he used them on grain jars. though he could be lying I mean there was that thousand dollar wet fiasco and all

not exactly a trustworthy guy and not the first TC I would take advice from but it's been done before, it can be done again

Quote:

hamloaf said:
Quote:

Rosen_Rot said:
The other jars, the ones that will be used in G2G will have SFDs even though, from my perspective, they are mainly used for needles but they still hold important GE rates so it should work fine.



Those aren't synthetic filter disks then.  Those are Whatman filters that you speak of.  They are syringe filters, but have been adopted in this hobby for the small time cultivator to be able to shake LC's by hand without getting the filter wet.  Whatman filters work ok for quart grain jar colonization though.




Quote:

hamloaf said:
Quote:

Right now they have silicone injection ports and a hole with Micropore tape over it.



Micropore tape alone cannot be used as a proper GE filter.  You will need to couple your micropore tape with tyvek and double it up over your GE holes,


or


Use polyfil as someone above keeps sqwaking about.  Polyfill will make a fine filter for GE on grain jar lids.  Tyvek and micropore tape better.  Best, synthetic filter disks and Whatman syringe filters rated down to 2 micrometers (or "um").  No matter what filter you decicde to choose, be sure to cover your lids with aluminum foil sheets upon the PC cycle so that no filter material becomes wet from the raining condensation of the lid of your PC cycle.
LM1




Quote:

hamloaf said:
Quote:

Edmunter said:
I have just gone out and bought myself some lovely new quart jar.  These kilners are the nuts but I wanted a general opinion on what is the best way to add a filter?

1. Drill hole and stuff with polyfil
2. Drill a few holes and cove with tyvek
3. Or use a syringe filter
4.Anything else




If these are the only materials you have on hand, currently, the best filter that could be made out of the materials listed for grain jars is tyvek.  Just be sure to double up the tyvek over the filter duct. 

Syringe filters aren't the best for grain jars, but they do work in a pinch.  If you plan on down the road dabbling in liquid cultures, save your syringe filters for future liquid culturing endeavors.




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Invisibleazur
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Re: Got the wrong lids [Re: bodhisatta]
    #23522812 - 08/08/16 06:51 PM (7 years, 5 months ago)

:shrug:


--------------------


A cube is NOT a cube.

FALL IN LOVE WITH LC
FOTTSE!!!
ALL NOOBS READ THIS!!!



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InvisiblePastywhyteMDiscord
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Re: Got the wrong lids [Re: azur]
    #23522877 - 08/08/16 07:16 PM (7 years, 5 months ago)

When I did GLC last I used the whatman's. But I also unscrewed the lids a touch when it looked like it was in danger of stalling.


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