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OfflineRealmLAir
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Registered: 07/17/16
Posts: 94
Last seen: 2 years, 8 months
3rd times the Charm???
    #23520790 - 08/07/16 11:56 PM (7 years, 5 months ago)

So i guess this is my 3rd attempt...it seems.

1st try-(7/15) 6 1/2 pint Jars Pf Tek, NO PC, Straight Spore Syringe Inoculation, left on the foil lids...asked why i wasnt getting growth and was told foil wasnt necessary to be left on "Anybody who told you otherwise is probably a noob". Now im getting opposing advice, "why did you take the foil off"...help here. Foil lids on or off?

These are still incubating, I checked them today and 1 jar showed two spots of green mold. threw it out immediately. 5/6 are still sitting in my cupboard, foil lids off, only a dry verm barrier, without any sign of growth. im thinking its because i didnt have a PC.

2nd try-(7/25) made 2 jars of LC (karo tek) using my only source, a spore syringe...But was told it was a terrible idea. Still I Saw good LC growth, so I Inoculated a jar (after getting a PC) and am seeing (what i think is) very good healthy mycelium growth.

I still have the other LC jar from the same batch that i kept in my closet. I would say its looking pretty good, is it of any use or no?

3rd try--(8/7) I followed Pastys easy agar tek. Have 8 mini rounds in the PC cooling overnight. tomorrow morning im putting a drop or two each from my spore syringe then pray for the best.

Question: Is it a stupid idea to use the other LC i made on an agar dish? and if so can you explain why?


Edited by RealmLAir (08/07/16 11:59 PM)


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InvisiblePastywhyteMDiscord
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Re: 3rd times the Charm??? [Re: RealmLAir]
    #23520798 - 08/08/16 12:01 AM (7 years, 5 months ago)

You should test every LC on an agar dish. That is what we use to determine if an LC is clean to use.


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OfflineRealmLAir
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Registered: 07/17/16
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Re: 3rd times the Charm??? [Re: Pastywhyte]
    #23520803 - 08/08/16 12:03 AM (7 years, 5 months ago)

So the agar dishes should be used for the LC or the spore syringe? Or should I do both?


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Offlineglbggg
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Registered: 04/22/16
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Re: 3rd times the Charm??? *DELETED* [Re: RealmLAir]
    #23520814 - 08/08/16 12:07 AM (7 years, 5 months ago)

Post deleted by glbggg

Reason for deletion: caszsadf



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goodbye cruel world


Edited by glbggg (08/13/16 05:24 PM)


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OfflineRealmLAir
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Re: 3rd times the Charm??? [Re: glbggg]
    #23520834 - 08/08/16 12:17 AM (7 years, 5 months ago)

So the growth that's in that jar with no lid whatsoever is no good or is it just susceptible to contamination?


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Offlineglbggg
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hhsdfazh *DELETED* [Re: RealmLAir]
    #23520838 - 08/08/16 12:19 AM (7 years, 5 months ago)

Post deleted by glbggg

Reason for deletion: im gay



Edited by glbggg (09/10/16 07:23 PM)


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InvisiblePastywhyteMDiscord
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Re: 3rd times the Charm??? [Re: glbggg]
    #23521226 - 08/08/16 06:24 AM (7 years, 5 months ago)

You were probably told to remove the foil by someone who didn't realize that you had no lids under the foil. Using foil as a lid is a super ghetto and inefficient method that was largely abandoned years ago. We don't see that much today. Those are probably screwed, without a lid of any kind they will likely dry out if they don't contam.

Keeping foil on when you are using a proper lid set up is a bad thing because it causes the dry verm layer to get wet. That allows stuff to grow though it. The person who told you to remove the foil was trying to help, assuming a proper lid set up, his advice would have been correct.

Don't toss them or anything tho, they still might make it.


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OfflineMushierage
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Re: 3rd times the Charm??? [Re: Pastywhyte]
    #23521235 - 08/08/16 06:35 AM (7 years, 5 months ago)

Staaaaaaaahp not using lids people......Why would this ever ever ever be a good idea

Sorry you were misinformed but why on earth wouldn't you use a proper lid on a -mason jar-    The friggen things are designed to have lids on them.


--------------------
Don't like researching posts? Read this! . Also, if you're new and your posts contain the words: Humidifer, incubator, air-stone, or heater, then you need to read and UTFSE before asking people to review your setup.  OR... You should be cultivating reptiles and fish, not mushrooms.



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Invisibledankington
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Re: 3rd times the Charm??? [Re: Mushierage]
    #23521417 - 08/08/16 09:06 AM (7 years, 5 months ago)

what I don't understand is, why wouldn't you have a lid? why do I have effin' totes of lids just chillin', never been touched?

Because the damn things come with lids! Any extras I bought and made left the others just sitting there. I probably have 50+ lids I'll never use…

Is it really so hard to poke a few holes in a lid?


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