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OfflineShuckahman
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Registered: 08/07/16
Posts: 1
Last seen: 7 years, 3 months
Panaeolus cyanescens
    #23520550 - 08/07/16 10:14 PM (7 years, 5 months ago)

Finally got to joining the Shroomery and I can't wait to make my contributions to this awesome community.

Anyway, I've been lurking around for more information on this subject but unfortunately there's not much I can find reguarding it. I rescently came across some panaeolus cyanescen Jamaican sub-strain spores and started them in my own mix of substrate (1/3 rye grain 1/3 bird seed and 1/3 organic compost) that is resistant to bacteria contamination (cooked once for 30 mins and again 3 days later for 60 mins).

The inoculation occurred in my glove box; they were incubated in a dirty garage with temps ranging from 75-90, 2 out of 10 jars got contaminated with green mold (I suspect that it had something to do with my syringe but I'm open to interpretation) I waited 1 month after full colonization to fruit, then an extra 3 days untill they bounced back to case. My casing was a 50\50 mix of verm and peat put in the pressure cooker for no more than 5 mins after the internal pressure was up, I waited 1 hour until repeating this process one more time to pasturize.

So it's been a few days now and most of them have green mold on the very top of their 1/4in casing layer, I used the same casing on my cubensis and still have no sign of contamination. I don't know where I went wrong, I saved a couple more jars in case I would end up botching my first attempt. All the jars look perfect, not a sign of contamination anywhere and before I start these guys I would like to get some advice from a more experienced member to help me prevent this from happening again.


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Everything I say is hypothetical, not a word is litteral.


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Offlinespacechildo
proletarians rise up
Male User Gallery

Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
Re: Panaeolus cyanescens [Re: Shuckahman]
    #23521775 - 08/08/16 11:36 AM (7 years, 5 months ago)

there's a whole thread dedicated to paan cyans https://www.shroomery.org/forums/showflat.php/Number/22216410/fpart/1
you need to pressure cook grains, qt jars goes at 15 psi for 90mins at least. you need to start over.

pasteurization isnt PC for 5 mins, its 140-160F core temp 60mins usually. manure a little longer, peat a little less maybe.


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