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SayinBud
420Magic

Registered: 07/14/16
Posts: 17
Last seen: 6 years, 9 months
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Anyone know what I've done wrong ??
#23518308 - 08/07/16 10:06 AM (7 years, 5 months ago) |
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So I followed the pf tek for simple minds. Used the ratio of 140ml verm to 40 ml of BRF per half pint pot. Sterilised my working area. Used sterile gloves and mouth mask as well as hat to cover my hair. When I put them in the PC I placed the lid on top but didn't fully close them shut so it clicked, but then covered them with double layered foil. Had them in the pressure cooker for an hour. Let them cool. Then inoculated as per pf tek.
They've then been sat in a box (in pictures) at temp of 23-28C, but more often then not its at 25-27C. Box covered with towels in to keep warm.


It's now the 8th day and there is only this much progression on 2 jars. The other 4 don't have any signs of growth at all.


Is there something I'm doing wrong or am I just been too impatient at this point I only ask cos most places I read on here show greater growth by this point.
I personally think it's either the ratio of BRF to verm or the fact I didn't seal the lids on the jars before placing in PC
What do you guys think ?
Thanks Bud
-------------------- New to this. Help is what I need
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impatientguy
Ganjalf a very mighty lab wizard



Registered: 11/26/14
Posts: 5,054
Loc: USA
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Re: Anyone know what I've done wrong ?? [Re: SayinBud]
#23518317 - 08/07/16 10:09 AM (7 years, 5 months ago) |
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Get them out of the box and into some ambient light and room temperature.
And wait some more.
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mupetmower
Mower of Muppets



Registered: 03/29/16
Posts: 3,036
Loc: Here and There
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Re: Anyone know what I've done wrong ?? [Re: SayinBud]
#23518318 - 08/07/16 10:09 AM (7 years, 5 months ago) |
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where to start... for one 8 days is nothing. be patient. it can take 2 weeks for spores to germinate and show growth, sometimes.
secondly, take them out of that box. you dont need to "incubate" them or anything. normal room temp is perfect, and light is beneficial at all stages. just putt hem on a shelf or something. no box, no heat mot, no towels, none of that.
also, how exactly did you inoculate these? i see they were the filter patch type lid things.. so did you open the jars in open air and inoc with syringe or what?
-------------------- -The wise man never stops seeking knowledge.
-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.
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ichugwindex
Dex



Registered: 06/04/16
Posts: 4,613
Loc: ¯\_(ツ)_/¯
Last seen: 11 months, 2 days
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Re: Anyone know what I've done wrong ?? [Re: SayinBud]
#23518320 - 08/07/16 10:10 AM (7 years, 5 months ago) |
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They need FAE take them out the box and set them on a shelf. You have holes in the lids right?
-------------------- Only hope can give rise to the emotion we call despair. But it is nearly impossible for a man to try to live without hope, so I guess that leaves Man no choice but to walk around with despair as his companion.
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Mushierage
SWIM Sinker



Registered: 06/29/16
Posts: 1,094
Last seen: 7 years, 1 month
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Re: Anyone know what I've done wrong ?? [Re: SayinBud]
#23518321 - 08/07/16 10:10 AM (7 years, 5 months ago) |
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This is normal. Jars can take up to 14 days to show signs of colonization.
Make sure you colonize on a shelf somewhere with ambient light (not direct, and not completely dark either) and colonize at around 75-78 F. You can colonize at lower temps and it will resist contamination but it will take longer.
Are you giving your jars proper Gas exchange or are they sealed completely?
-------------------- Don't like researching posts? Read this! . Also, if you're new and your posts contain the words: Humidifer, incubator, air-stone, or heater, then you need to read and UTFSE before asking people to review your setup. OR... You should be cultivating reptiles and fish, not mushrooms.
  
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Mushierage
SWIM Sinker



Registered: 06/29/16
Posts: 1,094
Last seen: 7 years, 1 month
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Re: Anyone know what I've done wrong ?? [Re: ichugwindex]
#23518327 - 08/07/16 10:12 AM (7 years, 5 months ago) |
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Quote:
ichugwindex said: They need FAE take them out the box and set them on a shelf. You have holes in the lids right?
Incorrect. They need GE. Gas Exchange. Please look up the difference before trying to give advice on it.
-------------------- Don't like researching posts? Read this! . Also, if you're new and your posts contain the words: Humidifer, incubator, air-stone, or heater, then you need to read and UTFSE before asking people to review your setup. OR... You should be cultivating reptiles and fish, not mushrooms.
  
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SayinBud
420Magic

Registered: 07/14/16
Posts: 17
Last seen: 6 years, 9 months
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Re: Anyone know what I've done wrong ?? [Re: Mushierage]
#23518351 - 08/07/16 10:24 AM (7 years, 5 months ago) |
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Thanks for the quick responses
Ok so I've put them on top of my drawer in my room as you guys said in normal light etc.
I made small holes with an old needle in lids before I started the whole process.. Inoculated through them holes then taped em up. That filter tape you can see in the middle of the lid was already there. Supposedly to help gas exchange. So apart from that tape. It's fully sealed
-------------------- New to this. Help is what I need
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Mushierage
SWIM Sinker



Registered: 06/29/16
Posts: 1,094
Last seen: 7 years, 1 month
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Re: Anyone know what I've done wrong ?? [Re: SayinBud]
#23518359 - 08/07/16 10:27 AM (7 years, 5 months ago) |
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Next time, I would make about a 1/4 inch hole in addition to inoc holes, and stuff with poly, or use a synthetic filter disc on top. This will greatly help GE and make your jars colonize in no time.
-------------------- Don't like researching posts? Read this! . Also, if you're new and your posts contain the words: Humidifer, incubator, air-stone, or heater, then you need to read and UTFSE before asking people to review your setup. OR... You should be cultivating reptiles and fish, not mushrooms.
  
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SayinBud
420Magic

Registered: 07/14/16
Posts: 17
Last seen: 6 years, 9 months
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Re: Anyone know what I've done wrong ?? [Re: Mushierage]
#23518526 - 08/07/16 11:19 AM (7 years, 5 months ago) |
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Doesn't the filter tape I have on the lids give enough GE anyway
I thought that was the point in them ??
-------------------- New to this. Help is what I need
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Anyone know what I've done wrong ?? [Re: SayinBud]
#23518549 - 08/07/16 11:25 AM (7 years, 5 months ago) |
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You made a hole in the lid with a needle then covered it up with micropore tape? Do you have a dry verm layer? Sounds bad if you don't.
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SayinBud
420Magic

Registered: 07/14/16
Posts: 17
Last seen: 6 years, 9 months
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Re: Anyone know what I've done wrong ?? [Re: Pastywhyte]
#23518625 - 08/07/16 11:43 AM (7 years, 5 months ago) |
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No the filter tape is in the middle already on the lid
I made holes with the needle more to the sides of the lids to put the spore liquid from the syringe against the inside wall of the jars, then covered with sellotape.
And yes I have a dry verm layer. But not sure if having the lids just resting on top during sterilisation has caused the water ratio to go off as well as moistening the dry layer
Thanks Bud
-------------------- New to this. Help is what I need
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Anyone know what I've done wrong ?? [Re: SayinBud]
#23518653 - 08/07/16 11:48 AM (7 years, 5 months ago) |
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Okay well then just be patient.
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spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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Re: Anyone know what I've done wrong ?? [Re: Pastywhyte]
#23518760 - 08/07/16 12:23 PM (7 years, 5 months ago) |
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GE is definitely not a problem with those strips, but those strips are pretty uneccessary for a pf-tek grow IMO. especially when you have to poke holes right next to it.
www.mushroomvideos.com is the pf-tek you wanna follow, dont bother incubating etc.
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SayinBud
420Magic

Registered: 07/14/16
Posts: 17
Last seen: 6 years, 9 months
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Re: Anyone know what I've done wrong ?? [Re: spacechildo]
#23518787 - 08/07/16 12:33 PM (7 years, 5 months ago) |
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Thanks guys !!
-------------------- New to this. Help is what I need
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SayinBud
420Magic

Registered: 07/14/16
Posts: 17
Last seen: 6 years, 9 months
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Re: Anyone know what I've done wrong ?? [Re: SayinBud]
#23520176 - 08/07/16 08:11 PM (7 years, 5 months ago) |
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What strains do you guys use with this tek and what ratio of verm to BRF do you use ??
-------------------- New to this. Help is what I need
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Anyone know what I've done wrong ?? [Re: SayinBud]
#23520279 - 08/07/16 08:38 PM (7 years, 5 months ago) |
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Use psilocybe cubensis. You don't know what strains you have. Every two spores that successfully mate is a strain. If you used a spore syringe you've got 100s of strains growing. Some mushrooms will be made of multiple strains Any variety of cubensis can be mixed with any other spores don't know they have a name
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SayinBud
420Magic

Registered: 07/14/16
Posts: 17
Last seen: 6 years, 9 months
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Re: Anyone know what I've done wrong ?? [Re: bodhisatta]
#23524250 - 08/09/16 08:26 AM (7 years, 5 months ago) |
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All I know is that the name of the spore syringe was Ecuador
Just wondering what others do
-------------------- New to this. Help is what I need
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spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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Re: Anyone know what I've done wrong ?? [Re: spacechildo]
#23524259 - 08/09/16 08:32 AM (7 years, 5 months ago) |
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most of us do grains and bulk subs but for cakes:
Quote:
spacechildo said: www.mushroomvideos.com is the pf-tek you wanna follow
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Anyone know what I've done wrong ?? [Re: bodhisatta]
#23524693 - 08/09/16 11:41 AM (7 years, 5 months ago) |
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Quote:
Trusted Cultivator said: Use psilocybe cubensis. You don't know what strains you have, because every two spores that successfully mate is a strain. If you used a spore syringe you've got 100s of strains growing. Some mushrooms will be made of multiple strains. Any variety of cubensis can be mixed with any other cubensis. Spores don't know they have a name.
ftfy
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