|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
tump
ban the undead



Registered: 03/17/16
Posts: 2,383
Last seen: 6 years, 10 months
|
why is clean lc slurry not taught more.
#23517789 - 08/07/16 04:05 AM (7 years, 5 months ago) |
|
|
If a lc is made cleanly with agar culture. Why cant you made a quart of it. Let it grow out. Suck up a 10 ml
|
Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
|
Re: why is clean lc slurry not taught more. [Re: tump]
#23517799 - 08/07/16 04:20 AM (7 years, 5 months ago) |
|
|
What do you think people do with their LCs? Drink them?
If your question is, "why are LCs not used as much as they were in the past?", my answer would be because LI is faster to do and it carries none of the risks. Also, people in the past didn't know just how dirty their seemingly clean LCs were, lots of folks prefer to use wedges over LCs just to make sure they use the cleanest inoculant possible.
Wedges are awesome IMO, I used to be an LC evangelist, just like you. Until I realised that wedges are the shit.
Edit:
Also, inoculating a jar with LC is not called an "LC slurry", it's simply called "LC". A slurry is a watery solution that has a lot of solids in it, like a PF cake blended down to make a slurry.
|
bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
|
Re: why is clean lc slurry not taught more. [Re: tump]
#23517975 - 08/07/16 06:54 AM (7 years, 5 months ago) |
|
|
Quote:
tump said: If a lc is made cleanly with agar culture. Why cant you made a quart of it. Let it grow out. Suck up a 10 ml
That's exactly what people do dude...
In hundreds of threads
|
Mycologist217
Frank's Disciple



Registered: 02/13/13
Posts: 2,425
Loc: Man of the Moon
Last seen: 1 day, 7 hours
|
Re: why is clean lc slurry not taught more. [Re: bodhisatta]
#23518120 - 08/07/16 08:50 AM (7 years, 5 months ago) |
|
|
I'm currently in the fruiting stage of a grow I started with a LC. I inoculated 42 grain jars with about 2mls LC each and a couple with 5-10mls each. I did this with a rotation between a couple 10ml syringes! You can read the grow log HERE
-------------------- My LC Manual (With custom LC lid Tek) ~~ Required Mycology Supplies ~~ Agar Work Videos ~~ L G M AMU Q&A-NO SYMPATHY FOR THE DEVIL! KEEP THAT IN MIND! BUY THE TICKET: TAKE THE RIDE Check out my Retail Gourmet Mushroom Farm! Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.
|
spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
|
Re: why is clean lc slurry not taught more. [Re: tump]
#23518768 - 08/07/16 12:25 PM (7 years, 5 months ago) |
|
|
WTF is a LC slurry? you blend your LC after colonization??
|
mupetmower
Mower of Muppets



Registered: 03/29/16
Posts: 3,036
Loc: Here and There
|
Re: why is clean lc slurry not taught more. [Re: spacechildo]
#23518789 - 08/07/16 12:34 PM (7 years, 5 months ago) |
|
|
i dont think any of us really know what this guy does, is doing, is gonna do...
-------------------- -The wise man never stops seeking knowledge.
-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.
|
spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
|
Re: why is clean lc slurry not taught more. [Re: mupetmower]
#23518801 - 08/07/16 12:37 PM (7 years, 5 months ago) |
|
|
I've seen him mention doing LC -> LC slurry a cpl times before, figured its about time to ask!
|
tump
ban the undead



Registered: 03/17/16
Posts: 2,383
Last seen: 6 years, 10 months
|
Re: why is clean lc slurry not taught more. [Re: Mycologist217]
#23518849 - 08/07/16 12:55 PM (7 years, 5 months ago) |
|
|
Yes i recently have blender my lc and poured it. Very fast recovery. Normal lc takes me a week to recover. Same with blenderless li.
|
spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
|
Re: why is clean lc slurry not taught more. [Re: tump]
#23518865 - 08/07/16 12:58 PM (7 years, 5 months ago) |
|
|
you can't pour your LC normally? somethings VERY wrong with the way you go about things man... 1 week just to recover sounds crazy to me!
|
Mycologist217
Frank's Disciple



Registered: 02/13/13
Posts: 2,425
Loc: Man of the Moon
Last seen: 1 day, 7 hours
|
Re: why is clean lc slurry not taught more. [Re: spacechildo]
#23518875 - 08/07/16 01:02 PM (7 years, 5 months ago) |
|
|
Obviously there is a misuse of terminology going on, looks like English might not be OP's first language
-------------------- My LC Manual (With custom LC lid Tek) ~~ Required Mycology Supplies ~~ Agar Work Videos ~~ L G M AMU Q&A-NO SYMPATHY FOR THE DEVIL! KEEP THAT IN MIND! BUY THE TICKET: TAKE THE RIDE Check out my Retail Gourmet Mushroom Farm! Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.
|
Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
|
Re: why is clean lc slurry not taught more. [Re: Mycologist217]
#23519047 - 08/07/16 01:57 PM (7 years, 5 months ago) |
|
|
If your LCs are taking a week to recover, they are probably as bacterial as a public latrine. Whenever I used LCs in the past I saw recovery within 2-3 days. Any more than that and I don't even expect them to recover and just chuck em. The only thing that could take close to a week to recover would be blenderless LI if you don't get any agar pieces in your jar, anything else that takes that long is fucked.
You currently seem to have a totally scewed idea of your inoculants, undoubtedly because you are missing a whole lot of contams. I said this in your previous thread - Use wedges, learn slowly but surely, make clean grows, slowly start going back up the ladder until you are doing clean LCs that recover in the timeframe that they should be recovering in. You are currently doing a thousand step inoculation filled with all kinds of vectors, thinking it's the only way to get fast colonization (which is a fucking illusion if I ever saw one).
Whenever I use wedges, I get fully colonized grains within 2 weeks, if your LCs are taking 1 week to recover, wedges will actually be a step up, not down.
|
mupetmower
Mower of Muppets



Registered: 03/29/16
Posts: 3,036
Loc: Here and There
|
Re: why is clean lc slurry not taught more. [Re: Supalemonhaze]
#23519181 - 08/07/16 02:38 PM (7 years, 5 months ago) |
|
|
i just dont see how someone thinks adding more (poinless/useless) steps is going to help anything, especially the speed aspect.. it is just.. illogical.
-------------------- -The wise man never stops seeking knowledge.
-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.
|
Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
|
Re: why is clean lc slurry not taught more. [Re: mupetmower]
#23521077 - 08/08/16 03:30 AM (7 years, 5 months ago) |
|
|
I can't ever understand why people think they just need to use more inoculant when they get poor recovery time. It's literally accepting the fact that your inoculant is contaminated so instead of fixing the problem, you exacerbate it by using more contaminated inoculant.
Not trying to rub it in your face or anything trump, but this is the 2nd time you mentioned this in as many days and both times you were told by multiple people that your problem is contams, not lack of inoculant. Yet, you seem to completely ignore them, carrying on complaining about long recovery of mycelium and even ranting about why LCs aren't being recommended as much as they were in the past.
It seems to me that yours is a textbook begginer mistake(and entirely the reason why LCs are never recommended to beginners) where one keeps using something for the sake of it's speed yet knowing damn well it's what's making them fail, ultimately.
Don't worry though, this will be the last time I ever say anything like this to you. If you won't listen, I sure as shit don't intend to force you to, even if I could.
|
tump
ban the undead



Registered: 03/17/16
Posts: 2,383
Last seen: 6 years, 10 months
|
Re: why is clean lc slurry not taught more. [Re: Supalemonhaze]
#23521090 - 08/08/16 03:49 AM (7 years, 5 months ago) |
|
|
I don't know why your being so nasty to what im saying. I've been making clean lc form agar cuts for years. Most ended up being clean. Im not trying to ad more bad inoculate. I do vaule what you all think. I just say blendering the already grown out lc and light pouring works better as easier. Then stabbing a needle through a lc port and contaming that way. I haven't even been arguring with you.
|
Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
|
Re: why is clean lc slurry not taught more. [Re: tump]
#23521114 - 08/08/16 04:25 AM (7 years, 5 months ago) |
|
|
I just said in my post above that I am not being nasty. You can't really expect us to not get mad when you make us repeat ourselves.
The fact is that if your mycelium is taking a week to recover, your inoculant is not clean. You think that you have been doing clean LCs but surely, if they take a week to recover, it's not clean. If mycelium from an LC doesn't recover in 2-3 days, it is not clean. It's just that simple.
You keep saying that you have been doing "clean" LCs for years but not a single person is going to believe that if you then say that it takes a whole week to recover. Not a single one.
I get what you are saying about the port being a contam vector, I also agree with you on this. I have had the same results whenever I used ports but this is not the point of what I am saying.
Another thing that comes to mind is your grain prep. You can have a clean LC but if you over-hydrate your grains, you're fucked. I'm pretty sure that if you find out which one of these is the problem and fix it, you will start getting fully colonized jars within a week, instead of waiting a whole week to only see recovery.
As I have said in your other thread, when I use wedges, I get a colonized jar in 2 weeks. If your LC is clean, why is it taking as long as my wedges to colonize a grain jar?
|
tump
ban the undead



Registered: 03/17/16
Posts: 2,383
Last seen: 6 years, 10 months
|
Re: why is clean lc slurry not taught more. [Re: Supalemonhaze]
#23521809 - 08/08/16 11:51 AM (7 years, 5 months ago) |
|
|
Because when i was using a syringes to suck it up. I only inject 1ml into quarts. I know most of the time the reason i contaminate. About four months ago my jars started slowing down and contam before first flush. In all but one case it can be tace back to my failures in hand eye condornation. When my ports didn't fail while trying suck up lc. My jars took two weeks then spawn away. With my bad hand condernation i can even pour and small amount of li. Thats why i feel i have to blender everything and spigot it. In theory if it was clean before the blender i should be able to blend it and pour it through a spigot no problem. But hell even tranfer three agar wedges To more agar. I contam one or more of them.
|
spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
|
Re: why is clean lc slurry not taught more. [Re: tump]
#23521819 - 08/08/16 11:54 AM (7 years, 5 months ago) |
|
|
Quote:
tump said: But hell even tranfer three agar wedges To more agar. I contam one or more of them.
then you really need to focus on sterile technique, no point in making LC's if you cant even get clean growth on agar!
|
mupetmower
Mower of Muppets



Registered: 03/29/16
Posts: 3,036
Loc: Here and There
|
Re: why is clean lc slurry not taught more. [Re: spacechildo]
#23521831 - 08/08/16 12:00 PM (7 years, 5 months ago) |
|
|
lost cause.. another person who has got it alllll figured out..
-------------------- -The wise man never stops seeking knowledge.
-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.
|
spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
|
Re: why is clean lc slurry not taught more. [Re: spacechildo]
#23521843 - 08/08/16 12:07 PM (7 years, 5 months ago) |
|
|
well at least let me try I've met worse and think its just something that gets lost in translation somewhere.. cant let the dude have 300qt jars of dirty spawn!
|
tump
ban the undead



Registered: 03/17/16
Posts: 2,383
Last seen: 6 years, 10 months
|
Re: why is clean lc slurry not taught more. [Re: spacechildo]
#23521872 - 08/08/16 12:21 PM (7 years, 5 months ago) |
|
|
Mupet mower why you being so negative with people. At no point have i disagree with any of you guys. I haven't fliped out. I haven't firgure it out or i wouldn't be asking on here. I am working with sterile techniques. I just fail at them because i have only one goodish hand. And one that can't hold onto anything correctly.
|
spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
|
Re: why is clean lc slurry not taught more. [Re: tump]
#23521897 - 08/08/16 12:31 PM (7 years, 5 months ago) |
|
|
so now you're gonna try spores - agar - transfer until you get clean growth - put agar wedge into sterilized grain jar. right?
|
tump
ban the undead



Registered: 03/17/16
Posts: 2,383
Last seen: 6 years, 10 months
|
Re: why is clean lc slurry not taught more. [Re: spacechildo]
#23521915 - 08/08/16 12:37 PM (7 years, 5 months ago) |
|
|
Next round maybe i have 38 first tranfer plates and five spore geration plates that are four days old And the nikki clone with three first tranfers for her pin. I will try the next round with wedges but ill still come crying to you when it fails.
|
Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
|
Re: why is clean lc slurry not taught more. [Re: spacechildo]
#23521934 - 08/08/16 12:42 PM (7 years, 5 months ago) |
|
|
I feel like liquid methods are demonstrated lots. Hell for the 2015 AMU grow along I did all LC for my log. We have LI methods including both blended and blenderless. There is loads of information. Use the search function FTW.
If wedges are failing then you have something fundamentaly wrong going on. If the vector is improper grain sterilization all the LC in the world ain't going to help you. That's why people say agar, it eliminates some of the vectors allowing a careful cultivator to nail down the problem.
|
mupetmower
Mower of Muppets



Registered: 03/29/16
Posts: 3,036
Loc: Here and There
|
Re: why is clean lc slurry not taught more. [Re: Pastywhyte]
#23521972 - 08/08/16 12:57 PM (7 years, 5 months ago) |
|
|
maybe youre right, space. sorry, tump. maybe it just seemed that way because of the language barrier.
-------------------- -The wise man never stops seeking knowledge.
-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.
|
|