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SoulButter
Joint Chief of Soul


Registered: 06/23/15
Posts: 312
Last seen: 3 years, 3 days
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Agar additives with anti-bacterial properties?
#23516331 - 08/06/16 03:49 PM (7 years, 5 months ago) |
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Ive seen some agar listed as "anti-bacterial" and just wondering what could be added to the mix to reduce bacteria contamination. I have purchased an MEA mix that was often getting contaminated, and im about to try again.
Also, can i surface sterilize some mycelial tissue pinched from a colonized tub and inoculate agar with that??
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PsilocyBen17
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Registered: 10/20/13
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Re: Agar additives with anti-bacterial properties? [Re: SoulButter]
#23516333 - 08/06/16 03:52 PM (7 years, 5 months ago) |
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I posted this in a thread just an hour ago....
Quote:
PsilocyBen17 said: Congrats on getting into agar OP! IMO its the most important part of this hobby. I would recommend starting with regular agar. Pastywhites Easy Agar Tek is highly recommended:
https://www.shroomery.org/forums/showflat.php/Number/19208976
Pasty is the king of agar and most of what I know comes from the contributions he has made...
I hesitate to recommend the antibiotic stuff. Antibiotic agar is really only for cloning exceptionally dirty fruits from the wild. It may work for your purposes, but If you use it all the time it will become a crutch preventing you from developing good technique.
If you do chose to go with antibiotic agar, make sure you transfer to regular agar before going to grains. This will ensure that the culture is actually clean and that you haven't just knocked the bacteria down enough to allow it to hitch a ride.
In the future you will get more replies if you post in Mushroom Cultivation....this isn't really an advanced topic.
Pb
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SoulButter
Joint Chief of Soul


Registered: 06/23/15
Posts: 312
Last seen: 3 years, 3 days
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Re: Agar additives with anti-bacterial properties? [Re: PsilocyBen17]
#23516342 - 08/06/16 03:56 PM (7 years, 5 months ago) |
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Thank you, im well familiar with the tek. I think the problem was contaminated inoculant, so I wanted to explore other options of agar. I see what youre saying though, dont want any sleepers gettin in me grains.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Agar additives with anti-bacterial properties? [Re: PsilocyBen17]
#23516350 - 08/06/16 03:59 PM (7 years, 5 months ago) |
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Quote:
Trusted Cultivator said: Generally you want to not use antibacterial agar so that you can try to grab some mycelium and move it to a new dish avoiding the bacteria. If it grows you can see it. Antibacterial agar isn't fool proof you can bring bacteria along still and then it thrives when you move it to spawn. Use antibacterial agar to help with wild fruit spores/clones. But it certainly doesn't make up for techniques which 9 out of 10 times is the problem that antibacterial agar isn't the solution to
Quote:
RogerRabbit said: Antibiotic agar will 'help' to prevent bacteria in your culture, but is by no means a cure all. I use the antibiotic agar from fungi.com and fungi spores both perfect and imperfect germinate just fine on it. Bacteria, when it's in sufficient quantities will also germinate and grow on it. What the antibiotic agar does is slow the bacteria down enough to help you make transfers away from the contamination into a fresh dish that also has antibiotic agar. Hopefully, your mushroom strain is strong enough to outrun the bacteria.
If you have a clean print or syringe, there is no need to use the antibiotic agar. I keep both on hand, but only use the antibiotic when absolutely necessary. RR
Relying on antibiotic agar is a great way to get sleepers into your grains
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PsilocyBen17
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Re: Agar additives with anti-bacterial properties? [Re: SoulButter]
#23516354 - 08/06/16 04:01 PM (7 years, 5 months ago) |
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If you are seeing contaminations its likely your technique, not the recipe you are using. Don't sweat it though man, your technique will improve with practice and agar is bound to help you even if its slow going at first....
I find both of these threads incredibly helpful for improving technique.
https://www.shroomery.org/forums/showflat.php/Number/23130868
https://www.shroomery.org/forums/showflat.php/Number/20111637#20111637
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Agar additives with anti-bacterial properties? [Re: PsilocyBen17]
#23516358 - 08/06/16 04:03 PM (7 years, 5 months ago) |
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I've never seem a vendor syringe so bad it would require the use of antibiotics in agar.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Agar additives with anti-bacterial properties? [Re: bodhisatta]
#23516361 - 08/06/16 04:06 PM (7 years, 5 months ago) |
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IMO antibiotics is not needed with cubes. They are strong enough that a sandwich or warm pour is enough. Maybe for Hericium clones or wild spores antibiotics are helpful. But not cubes.
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SoulButter
Joint Chief of Soul


Registered: 06/23/15
Posts: 312
Last seen: 3 years, 3 days
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Re: Agar additives with anti-bacterial properties? [Re: Pastywhyte]
#23516387 - 08/06/16 04:20 PM (7 years, 5 months ago) |
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That settles it, sticking to standard MEA. Does anyone have input on the second question? Is there any reason not to inoculate agar plates with mycelium pinched from a minitub and then surface sterilized?
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Agar additives with anti-bacterial properties? [Re: SoulButter]
#23516446 - 08/06/16 04:42 PM (7 years, 5 months ago) |
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yes clone a fruit don't take mycelium from a substrate
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PsilocyBen17
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Registered: 10/20/13
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Re: Agar additives with anti-bacterial properties? [Re: bodhisatta]
#23516482 - 08/06/16 04:55 PM (7 years, 5 months ago) |
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The inside of a fruit is much cleaner than thw surface of a substrate
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