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mothballs
Stranger in a strange land



Registered: 04/30/10
Posts: 242
Last seen: 5 years, 9 months
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bacteria blues
#23515549 - 08/06/16 10:00 AM (7 years, 5 months ago) |
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I've been having bacteria problems characterized by stalled jars, rotten fruit smell, and weak mycelia. i use pf tec on small jelly jars w masking tape. i sterilize my needles w a propane blowtorch between jars. frankly, im at a loss. at times the method is almost flawless, on other occasions, bacteria is rampant(80%). id really appreciate any suggestions to prevent this. Would H2o2 in the syringe or jar work?
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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H2o2 would hurt. It damages mycelium helping contamination out.
Pictures or explanation of everything start to finish would help
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Agar for syringe cleanup. Every project should be started on agar anyways. Also why masking tape? How permeable is it? You need a dry verm layer. That's it. No tape necessary.
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Mushierage
SWIM Sinker



Registered: 06/29/16
Posts: 1,094
Last seen: 7 years, 1 month
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Yeah, give us a detailed guide of your sterile tek, spawn prep, and inoculation. Procedure. We can help you out but we need more information.
-------------------- Don't like researching posts? Read this! . Also, if you're new and your posts contain the words: Humidifer, incubator, air-stone, or heater, then you need to read and UTFSE before asking people to review your setup. OR... You should be cultivating reptiles and fish, not mushrooms.
  
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mothballs
Stranger in a strange land



Registered: 04/30/10
Posts: 242
Last seen: 5 years, 9 months
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been useing masking tape for many years.... it works....pics wig me out
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Mushierage
SWIM Sinker



Registered: 06/29/16
Posts: 1,094
Last seen: 7 years, 1 month
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Quote:
Mushierage said: Yeah, give us a detailed guide of your sterile tek, spawn prep, and inoculation. Procedure. We can help you out but we need more information.
No pics needed then. Just write out your process.
-------------------- Don't like researching posts? Read this! . Also, if you're new and your posts contain the words: Humidifer, incubator, air-stone, or heater, then you need to read and UTFSE before asking people to review your setup. OR... You should be cultivating reptiles and fish, not mushrooms.
  
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mothballs
Stranger in a strange land



Registered: 04/30/10
Posts: 242
Last seen: 5 years, 9 months
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1 part brf, 2parts verm, 1 part water in 1/2 cup mason jelly jars inoculated at 4 points (old school PF tec) with a dry verm barrier....pc-ed at 15 psi for for 1hr plus and knocked up as soon as cool. i use a propane blow torch to flame sterilize between jar innoculations
Edited by mothballs (08/06/16 10:13 AM)
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Then explain your inoc procedure. Please tell me you don't use alcohol to wipe your needle.
Although posting a pic isn't bad.. lol@wigs you out. This site is HTTPS and exif free, as well as protected by the 5th.
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Mushierage
SWIM Sinker



Registered: 06/29/16
Posts: 1,094
Last seen: 7 years, 1 month
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I'm not asking for your sub ratios. I'm asking for a detailed list that tells me your inoculation and sterile tek process.
Can't help you figure out contam vectors if you do not provide this info.
-------------------- Don't like researching posts? Read this! . Also, if you're new and your posts contain the words: Humidifer, incubator, air-stone, or heater, then you need to read and UTFSE before asking people to review your setup. OR... You should be cultivating reptiles and fish, not mushrooms.
  
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Keep foil on lids or keep foil off after inoculation We mean details
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mothballs
Stranger in a strange land



Registered: 04/30/10
Posts: 242
Last seen: 5 years, 9 months
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i use hand sanitizer,blue nitril gloves and a dustmask to innoc, pull the tape back, shoot em up, and cover back up. i leave the jars in a box in the closet till colonized. no finger fucking the jars. checked once every 4-5 days. i dont really mess w the temperature, my closet stays at about 75-78 degrees
Edited by mothballs (08/06/16 10:20 AM)
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Mushierage
SWIM Sinker



Registered: 06/29/16
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Last seen: 7 years, 1 month
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Are you inoculating in open air? This is a contam vector.
You are innoculating directly from spores to grain. This is also a contamination vector. To remove this vector, inoc to agar, then do agar transfers if necessary (most times it's not necessary) and inoc with agar wedge.
-------------------- Don't like researching posts? Read this! . Also, if you're new and your posts contain the words: Humidifer, incubator, air-stone, or heater, then you need to read and UTFSE before asking people to review your setup. OR... You should be cultivating reptiles and fish, not mushrooms.
  
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Mushierage
SWIM Sinker



Registered: 06/29/16
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Last seen: 7 years, 1 month
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Also, 9 times out of 10 when you have bacteria, it's already in your spawn before even inoculating. PCING for 1 he isn't long enough. PC for 90 mins minimum. Use foil over the lid to prevent anything falling onto it, and try inoculating in a still air box or in front of a flow hood.
-------------------- Don't like researching posts? Read this! . Also, if you're new and your posts contain the words: Humidifer, incubator, air-stone, or heater, then you need to read and UTFSE before asking people to review your setup. OR... You should be cultivating reptiles and fish, not mushrooms.
  
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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This is pf tek. No grains and no wedges.
Open air, although isn't ideal, won't be the reason why mass amounts of pf jars are going bacterial. Pf tek sterile tek is balls easy. If you don't alcohol wipe the needle, and flame between each jar, the problem is the syringe itself. Agar clean up and inoculating with LI would be the fix. Besides every project should be started on agar anyways.
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mothballs
Stranger in a strange land



Registered: 04/30/10
Posts: 242
Last seen: 5 years, 9 months
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yes, open air. I've not advanced to agar yet... no flow hood.(working on it)... im a poor man, this is a hobby, not business... but im advancing slowly. i do bleach and clean everything surreptitiously
Edited by mothballs (08/06/16 10:25 AM)
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Inoculate in a still air box. Keep your jars on a shelf with ambient light not in a box in a closet
PC for 1 hour is fine for cakes.
The last thing you need is a flow hood. Build a SAB
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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A $5 tote with arm holes cut out is all you need for agar and cake inoc.
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Mushierage
SWIM Sinker



Registered: 06/29/16
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Last seen: 7 years, 1 month
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Ah, missed the pf tek part. The above info I gave is for grains, but sterility process still holds true.
-------------------- Don't like researching posts? Read this! . Also, if you're new and your posts contain the words: Humidifer, incubator, air-stone, or heater, then you need to read and UTFSE before asking people to review your setup. OR... You should be cultivating reptiles and fish, not mushrooms.
  
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mupetmower
Mower of Muppets



Registered: 03/29/16
Posts: 3,036
Loc: Here and There
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why have so many people, lately, been thinking they need a flow hood to do agar work?
OP, SAB works perfectly fine for agar work, and everything else.
-------------------- -The wise man never stops seeking knowledge.
-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.
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mothballs
Stranger in a strange land



Registered: 04/30/10
Posts: 242
Last seen: 5 years, 9 months
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Quote:
Trusted Cultivator said: Inoculate in a still air box. Keep your jars on a shelf with ambient light not in a box in a closet
PC for 1 hour is fine for cakes.
The last thing you need is a flow hood. Build a SAB
does light affect colonization rates??????????
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mupetmower
Mower of Muppets



Registered: 03/29/16
Posts: 3,036
Loc: Here and There
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i think i have heard a few poepel say that it does. regardless of whether it speeds colonization or not, light is beneficial at all stages of growth. mushrooms have a circadian similar to humans.
this is why we dont colonize in total darkness anymore. that was silly
-------------------- -The wise man never stops seeking knowledge.
-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.
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mothballs
Stranger in a strange land



Registered: 04/30/10
Posts: 242
Last seen: 5 years, 9 months
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Quote:
mupetmower said: why have so many people, lately, been thinking they need a flow hood to do agar work?
OP, SAB works perfectly fine for agar work, and everything else.
really?... i tried and failed. it was a miserably hard xperience anyway. i felt like a moon man working with nuclear waste tied down to a table sweating my hands off. i am trying to build a flow box when my filter gets here, but im still waiting
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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It's harder to use a FH. If you can't get it down in a SAB a FH isn't going to be of any help.
If the SAB was too confined you just didn't build it big and wide enough for yourself
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Quote:
mupetmower said: i think i have heard a few poepel say that it does. regardless of whether it speeds colonization or not, light is beneficial at all stages of growth. mushrooms have a circadian similar to humans.
this is why we dont colonize in total darkness anymore. that was silly
Quote:
RogerRabbit said:
Quote:
Diesel_Dawg said: I've never done a back to back of light vs dark.. But I have done direct vs indirect, and the direct light significantly slowed growth.
This is correct. You want low level light during colonization.
I've repeated this experiment a few thousand times now. The colonizing bags in our mushroom farm near the north facing window grow at least 1/3 faster than those in the areas where it's darker. Also, substrates colonizing near the isle where light penetrates colonize faster.
With most species you can inoculate agar and then stack two dishes on top of each other. The top one will nearly always colonize faster than the bottom one.
What you don't want is very bright light during colonization which not only slows down growth but also could lead to premature fruiting. RR
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mothballs
Stranger in a strange land



Registered: 04/30/10
Posts: 242
Last seen: 5 years, 9 months
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you are right. there was alot wrong w my SAB, and maybe my agar as well. i just wanted to try it the RIGHT way before i tweeked my methods
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mothballs
Stranger in a strange land



Registered: 04/30/10
Posts: 242
Last seen: 5 years, 9 months
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thanks for the light tip
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mupetmower
Mower of Muppets



Registered: 03/29/16
Posts: 3,036
Loc: Here and There
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hmm im so glad you posted that RR quote, Mad! i wonder if maybe these last 12 jars i did with my GT clone all started pinning in-vitro so early because they had too much direct light..
the light isnt literally directly on them, but i would say its probably more than what you would want when you hear "indirect/ambient light".
going to need to get them a little farther away.
-------------------- -The wise man never stops seeking knowledge.
-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.
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