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Offlineleschampignons
Biochemistry + Mycology
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Registered: 08/30/13
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Culturing Chlorociboria species
    #23512239 - 08/05/16 10:08 AM (7 years, 5 months ago)

I have been trying to culture Chlorociboria species for a while now. I typically find the fruitbodies a couple of times a season, and put some of them to agar and watch a whole host of contamination spring forth. I have probably done this at least twice already. Getting a clean culture from these fruitbodies is extremely difficult because they are so small and cannot be effectively torn open to reveal a clean inner surface like most mushrooms.

I contributed to this thread a while ago. No pics, but descriptions. That thread also links to an older one, and you will probably find more threads about this fungus if you do a search.


A couple of days ago I found this fungus again on a small log. I brought the entire log home and put it in the fridge.
Today I washed a large (relative to this species normal size lol) fruitbody with mild soap and water and put it directly to agar. I was able to split the fruitbody and expose a decent portion of the inner fruit, but I was not  able to separate that from the outer part of the fruit, which almost certainly has bacteria and fungi tagging along on it. I made 5 plates in total. If I get a chance I will also put these on antibiotic agar to at least knock out bacterial contams. It is hard to take pics of my petris because they are mostly glass jars but if I get a chance I'll snap a few so you guys can see what I'm working with.


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Invisibleweetsie
unlicensed tub surgeon
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Registered: 05/08/11
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Re: Culturing Chlorociboria species [Re: leschampignons]
    #23512770 - 08/05/16 01:08 PM (7 years, 5 months ago)

Have you tried cardboard?

I had a p. cyan culture on wood chip that looked super healthy but the moment I put it on agar a mold shot across the plate within a day, It was the fastest growing mycelium I had ever seen, you could literally see the hyphae at the leading edge growing in real time.

Anyway point is I never got anywhere no matter how many times I tried, it contaminated the agar instantly.

On cardboard however, the contam didn't show up and the p. cyan mycelium colonized it without issue (even pinned after leaving it several months)

If you havn't tried already, drop a piece of tissue on brown corrugated cardboard, I just pour boiling water on it then transfer it to a petri dish when cool.


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Offlinepichardo
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Re: Culturing Chlorociboria species [Re: leschampignons]
    #23513160 - 08/05/16 03:07 PM (7 years, 5 months ago)

You can try peroxide or chlorine solution at 3% to clean mycelium tissue.


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Offlineleschampignons
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Re: Culturing Chlorociboria species [Re: leschampignons]
    #23563056 - 08/21/16 01:34 PM (7 years, 5 months ago)

This round gave rise to green mold (I think trichoderma sp.) contamination in every jar.

I still have a log with some fruitbodies in the fridge and they seem to be keeping well for an extended period in the fridge loosely wrapped in a plastic bag. This fungus dries out much more quickly than a normal mushroom and humidity must be kept very high to keep these fruitbodies from shriveling up. I may try again with a diluted bleach solution soon.


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Offlineleschampignons
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Re: Culturing Chlorociboria species [Re: leschampignons]
    #23732274 - 10/12/16 07:39 PM (7 years, 3 months ago)

I tried again a few weeks ago and it seems like a 10% bleach wash and plating to PDA + antibiotics **may** have worked. I am seeing slow growing staining fungus. Staining is very dark green - almost blackish Not sure if it is the desired culture, but nonetheless very interesting.


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Invisiblecosmicaug
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Re: Culturing Chlorociboria species [Re: leschampignons]
    #23735360 - 10/13/16 08:49 PM (7 years, 3 months ago)

Do you want to use it to stain wood?


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Offlinedrake89
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Re: Culturing Chlorociboria species [Re: cosmicaug]
    #23735381 - 10/13/16 08:54 PM (7 years, 3 months ago)

Yeah there's a university website that sells the culture for plugs

I was looking to see how much spalted wood goes for and couldn't find anywhere that even sells much of it


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OfflineQuadman
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Re: Culturing Chlorociboria species [Re: drake89]
    #23735685 - 10/13/16 10:54 PM (7 years, 3 months ago)

Spalted wood is all over eBay Drake. Sometimes fetches a premium. It is difficult line to get right. To little not enough pattern too much and it's rotten wood.


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Offlineleschampignons
Biochemistry + Mycology
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Re: Culturing Chlorociboria species [Re: cosmicaug]
    #23736965 - 10/14/16 12:12 PM (7 years, 3 months ago)

Yes I'd like to use it to stain wood. And perhaps some other materials as well.


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Offlinemyceliups
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Re: Culturing Chlorociboria species [Re: leschampignons]
    #24903876 - 01/10/18 07:52 PM (6 years, 20 days ago)

Considering the color pigment xylindein in this species,
is it possible it was staining the agar and just appearing
to be contaminated?

You could also get a spore print if you find them again.


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