Home | Community | Message Board

Cannabis Seeds UK
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: PhytoExtractum Kratom Powder for Sale   Left Coast Kratom Buy Kratom Extract   Bridgetown Botanicals Bridgetown Botanicals   North Spore Bulk Substrate   Original Sensible Seeds Autoflowering Cannabis Seeds   Mushroom-Hut Liquid Cultures   Kraken Kratom Kratom Capsules for Sale

Jump to first unread post Pages: 1 | 2 | 3 | 4 | 5 | 6 | 7  [ show all ]
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Offlinedextr0
Stranger
 User Gallery

Registered: 07/24/16
Posts: 431
Last seen: 20 days, 6 hours
My intro...
    #23511938 - 08/05/16 08:04 AM (7 years, 5 months ago)

OK I'll start off by saying I'm very ghetto right now...that means I have to be resourceful and make do. I'm making a log on all that I do and will be doing. As I progress more $ things will step up. But for now I make do. I have no prior experience I'm the cultivation of mushrooms, but  a experienced cannabis grower. I am or have been a member of countless cannabis forums and love to contribute and show people easy ways of doing things. My main interest was making plant fermented extracts for plants and soil management.
Just a little background...so I am here to learn and of course share experiences. I'm currently trying to use the cardboard method to try and get growth from a dried out mushroom. I mistakenly left one jar sealed up (baby food jar) and I noticed some wispy mycelia growing next to some healthy looking mycelia pieces ( of mushroom). Worried it might be cob web I transfered a piece of healthy looking mushroom to another jar with cardboard. This time I made a hole and stuffed poly in so it could breath. The other jar I set outside to see what happens. When I opened it last it had a rancid smell to it...?
With that said I decided cardboard is not for me right now experimentation can come later, right now I need for sure. So I broke down and went for agar agar. I picked up supplies yesterday and am going to be using agar, honey, dextrose, and brewers yeast. I also sent off to fSRE for a print. I have little to no money left so hopefully they send me my print need it.
Well that's my intro.
I've also got some Reishi that are dried and am going to see if they will work with either agar or cardboard.
I've got pics coming soon.
Any advice or input welcome..


Edited by dextr0 (08/05/16 08:17 AM)


Extras: Filter Print Post Top
Offlinedextr0
Stranger
 User Gallery

Registered: 07/24/16
Posts: 431
Last seen: 20 days, 6 hours
Re: My intro... [Re: dextr0]
    #23512035 - 08/05/16 08:46 AM (7 years, 5 months ago)

Can't seem to get pics on here...help anyone??


Extras: Filter Print Post Top
Offlinea_stoned_sloth
Gummy Bear Connoisseur

Registered: 05/17/16
Posts: 122
Loc: Parts Unknown
Last seen: 2 months, 24 days
Re: My intro... [Re: dextr0]
    #23512058 - 08/05/16 08:55 AM (7 years, 5 months ago)

Under your name in the top left corner it says Pics. This is where you can upload and share on the forum.


--------------------
"As the garden grows, so does the gardener."


Extras: Filter Print Post Top
Invisiblemupetmower
Mower of Muppets
Male User Gallery


Registered: 03/29/16
Posts: 3,036
Loc: Here and There
Re: My intro... [Re: a_stoned_sloth]
    #23512061 - 08/05/16 08:57 AM (7 years, 5 months ago)



--------------------
-The wise man never stops seeking knowledge.


-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.


Extras: Filter Print Post Top
OfflinePsykronik
OverGrower
 User Gallery

Registered: 02/28/12
Posts: 150
Loc: Scotland Flag
Last seen: 7 years, 4 months
Re: My intro... [Re: mupetmower]
    #23512066 - 08/05/16 08:59 AM (7 years, 5 months ago)

Where you ever a member of overgr.w before it got shut down ?


Extras: Filter Print Post Top
Offlinedextr0
Stranger
 User Gallery

Registered: 07/24/16
Posts: 431
Last seen: 20 days, 6 hours
Re: My intro... [Re: Psykronik]
    #23512075 - 08/05/16 09:05 AM (7 years, 5 months ago)



1. dry mushroom with cardboard. Wispy growth thought it might be cob web mold.
2. Reishi mushrooms on log. Want to try cardboard method. Haven't got around to it yet.
3. The other piece of mushroom (cubensis) that had what I think is good mycelial growth. Transfered it to another jar so wispy growth from other mushroom piece would not contaminate if it is cob web.


Edited by dextr0 (08/05/16 09:17 AM)


Extras: Filter Print Post Top
Offlinedextr0
Stranger
 User Gallery

Registered: 07/24/16
Posts: 431
Last seen: 20 days, 6 hours
Re: My intro... [Re: dextr0]
    #23512086 - 08/05/16 09:09 AM (7 years, 5 months ago)

Thanx everyone. Probably doing this the hard way but oh well.
I was never on OG, I have been on cannabis world, icmag, and thcfarmer. I still visit from time to time but have moved to different hobbies.


Extras: Filter Print Post Top
Invisiblemupetmower
Mower of Muppets
Male User Gallery


Registered: 03/29/16
Posts: 3,036
Loc: Here and There
Re: My intro... [Re: dextr0]
    #23512090 - 08/05/16 09:10 AM (7 years, 5 months ago)

so that is what, again? the cardboard with pieces of mushroom on it? what is the mushroom in the top right pic? if  that what you put on the cardboard? im lost here, sorry man haha.


--------------------
-The wise man never stops seeking knowledge.


-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.


Extras: Filter Print Post Top
InvisiblePastywhyteMDiscord
Say hello to my little friend
Male User Gallery


Registered: 09/15/12
Posts: 37,810
Loc: Canada Flag
Trusted Cultivator
Re: My intro... [Re: dextr0]
    #23512094 - 08/05/16 09:14 AM (7 years, 5 months ago)

That looks pretty gross. No telling what you have there. I suggest you get some spores from a reputable source and ditch the cardboard. Cardboard is great for low tek expansion of outdoor woodloving species. It's terrible for dunglovers that we are looking to bring indoors.


Extras: Filter Print Post Top
Invisibleoontribe
Male User Gallery

Registered: 01/14/15
Posts: 3,570
Re: My intro... [Re: mupetmower]
    #23512102 - 08/05/16 09:18 AM (7 years, 5 months ago)



Extras: Filter Print Post Top
Offlinedextr0
Stranger
 User Gallery

Registered: 07/24/16
Posts: 431
Last seen: 20 days, 6 hours
Re: My intro... [Re: Pastywhyte]
    #23512125 - 08/05/16 09:22 AM (7 years, 5 months ago)

I agree it looks gross. Wet cardboard does not have the appeal that agar has. Bottom line, I used a piece of dried mushroom...sandwiched between two cardboard pieces and waited for mycelial growth.
Until I get more funds I will have to make do. And thats what I'm doing.
I know this will work I just need time to work on it.


Extras: Filter Print Post Top
InvisiblePastywhyteMDiscord
Say hello to my little friend
Male User Gallery


Registered: 09/15/12
Posts: 37,810
Loc: Canada Flag
Trusted Cultivator
Re: My intro... [Re: dextr0]
    #23512144 - 08/05/16 09:29 AM (7 years, 5 months ago)

My advice is that if you can't afford a few bucks to do things in this hobby right, your priorities are misplaced. $100 or less can get you going on the PF tek. What you have going on there will be very hard pressed to expand into fruits.

Good luck.


Extras: Filter Print Post Top
Offlinedextr0
Stranger
 User Gallery

Registered: 07/24/16
Posts: 431
Last seen: 20 days, 6 hours
Re: My intro... [Re: Pastywhyte]
    #23512184 - 08/05/16 09:45 AM (7 years, 5 months ago)

OK how bout this. I'm going to mix up the agar. Then transfer a new piece of dried mushroom. That will make things pretty. I'm still going for the cardboard method in the other jars.
By the by...the Tek I'm using was explained by Roger Rabbit himself in past posts. I say this to add a little credibility to my findings. I'm not completely new to this. I've at least been doing my research.
Again Thanx to all who responded.


Extras: Filter Print Post Top
InvisiblePastywhyteMDiscord
Say hello to my little friend
Male User Gallery


Registered: 09/15/12
Posts: 37,810
Loc: Canada Flag
Trusted Cultivator
Re: My intro... [Re: dextr0]
    #23512204 - 08/05/16 09:53 AM (7 years, 5 months ago)

It will all be for nothing depending on how that mushroom was dried. You need to put stuff in context. If RR was talking about fruits specially grown in aseptic conditions and dried without heat (and that is likely what he was talking about) then yes, that can work. But if what your doing is taking a shroom ya bought from a dealer, literally smothered in contams, and was dried in a dehydrator to minimize the loss of actives, then no it won't work.


Extras: Filter Print Post Top
Offlinedextr0
Stranger
 User Gallery

Registered: 07/24/16
Posts: 431
Last seen: 20 days, 6 hours
Re: My intro... [Re: Pastywhyte]
    #23512237 - 08/05/16 10:07 AM (7 years, 5 months ago)

Let's get this straight...I have nothing to lose here and everything to gain. Truth is I have no idea how this mushroom was dried. My thought was, the stems have mycelial growth when dried. U break stem open its full of Cotton like mycelia. If I can get this to grow on agar or cardboard I will use that as spawn to inoculate my grain then move to monotub. Or at very least get me one spore print to work with. I understand the nessesity of sterilization and the nessesity of basically knowing for SURE your working with viable stuff. As in spores etc. Until I get spore print from FSRE I make do. It's also a cool experiment that will show others what may be possible for them.


Extras: Filter Print Post Top
InvisiblePastywhyteMDiscord
Say hello to my little friend
Male User Gallery


Registered: 09/15/12
Posts: 37,810
Loc: Canada Flag
Trusted Cultivator
Re: My intro... [Re: dextr0]
    #23512268 - 08/05/16 10:21 AM (7 years, 5 months ago)

The issue is whether the cells are dead or not. Nothing to do with what it looks like when you snap it open. Not to mention the level of contamination you are dealing with. If you have nothing better to do with your time then go for it. But the odds are greatly stacked against you.

Truthfully I'm not even sure that I could pull that one off. Maybe if it was the last penis envy in existence I might try it. But I wouldn't hope for much.

Good luck


Extras: Filter Print Post Top
Offlinedextr0
Stranger
 User Gallery

Registered: 07/24/16
Posts: 431
Last seen: 20 days, 6 hours
Re: My intro... [Re: Pastywhyte]
    #23512371 - 08/05/16 10:55 AM (7 years, 5 months ago)

Thank u for the reply pasty. I have a question...in the third picture u can see mycelial growth. In your opinion is this what I'm looking for? It is not at all wispy it is very thick like a cotton ball.


Extras: Filter Print Post Top
InvisiblePastywhyteMDiscord
Say hello to my little friend
Male User Gallery


Registered: 09/15/12
Posts: 37,810
Loc: Canada Flag
Trusted Cultivator
Re: My intro... [Re: dextr0]
    #23512528 - 08/05/16 11:42 AM (7 years, 5 months ago)

It's looking like mycelium. Of what species I cannot say. You might get really lucky. Ya never know. I'm just trying to temper your expectation somewhat and show you why we do things the way we do. Even if it is mycelium you certainly are going to have some bacteria riding along. I don't see how you will clean that up without agar. Maybe take it right to outdoors and hope for the the best. A bit of straw colonized with the cardboard then expanded to a small bed of straw and manure might work. Could maybe see a few grams like that.


Extras: Filter Print Post Top
Offlinedextr0
Stranger
 User Gallery

Registered: 07/24/16
Posts: 431
Last seen: 20 days, 6 hours
Re: My intro... [Re: Pastywhyte]
    #23512843 - 08/05/16 01:29 PM (7 years, 5 months ago)

My expectations are of course high as I'm the type of person to make things happen, especially in areas where I've taken good interest. I however know that odds are stacked against me. I am not advocating doing as I do, unless u are in circumstances similar to mine (broke but trying).
Cleaning the specimen I'm working with: this was addressed in RR's Tek that I am following. I have agar. I'm looking for a Tek that uses agar, honey, potato flakes, and brewers yeast. Problem is I just need to make a little so I'm working the math. I'm not good with math. With that said the Tek requires isolation of mycelia onto agar. Like you said the chances of contamination are high but with some work u should be able to isolate and eventually grow what you are looking for. Again on my part it will be luck and persistence if it does happen.


Extras: Filter Print Post Top
InvisiblePastywhyteMDiscord
Say hello to my little friend
Male User Gallery


Registered: 09/15/12
Posts: 37,810
Loc: Canada Flag
Trusted Cultivator
Re: My intro... [Re: dextr0]
    #23512903 - 08/05/16 01:45 PM (7 years, 5 months ago)

Okay well if you have agar this might work. Standard agar recipes for fungi have a few basics.

Need between 1.5-2.5% agar. 1.5% agar will make it really soft and is good for germinating spores.

2.5% agar is very firm. Good for isolation work.

2% is the standard. Works for most stuff.

Nutes need to consist of a starch and a simple sugar. You want between 1-2% starch and 0-2% simple sugar. Do not use more sugar than starch. Stuff like peptide or yeast are not needed but sometimes beneficial.

So a recipe like
1000ml water
20 grams agar
20 grams potato flakes
10ml honey

Would work just fine. Scale according to need


Extras: Filter Print Post Top
Offlinedextr0
Stranger
 User Gallery

Registered: 07/24/16
Posts: 431
Last seen: 20 days, 6 hours
Re: My intro... [Re: Pastywhyte]
    #23515916 - 08/06/16 12:28 PM (7 years, 5 months ago)

Ok so last night I made some agar. Not sure but think I might have fucked that one up. I brought it to a boil because i thought it all needed to be fully desolved. It wasn't scorched or anything but I needed a for sure so I looked around. Pasty, I ended up on your thread. Simple. I still wonder though did I cook long enough. ts not super clear but u can see through. I used telephone brand agar, and potato flakes....is this normal. I will post pictures ASAP.


Extras: Filter Print Post Top
Invisiblemupetmower
Mower of Muppets
Male User Gallery


Registered: 03/29/16
Posts: 3,036
Loc: Here and There
Re: My intro... [Re: dextr0]
    #23516218 - 08/06/16 02:54 PM (7 years, 5 months ago)

should be fine. i just made some MEA today, brought it to a boil and its fine. i usually bring my PDA, with the same agar you are using(telephone) to a near boil, and it's fine. when using that agar and potato flakes, i usually always have a tiny amount of "specs" in the agar. agar plates wont always be 100% transparent. they may be sorta slightly cloudy sometimes.


--------------------
-The wise man never stops seeking knowledge.


-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.


Extras: Filter Print Post Top
Offlinedextr0
Stranger
 User Gallery

Registered: 07/24/16
Posts: 431
Last seen: 20 days, 6 hours
Re: My intro... [Re: mupetmower]
    #23521634 - 08/08/16 10:33 AM (7 years, 5 months ago)

All jars turned out similar to this one. Looks like powder mildew or something. The other growth I'm getting looks flat and white. Sort of like spilled milk. I cant wait till I get some fuckin spores.


Extras: Filter Print Post Top
InvisiblePastywhyteMDiscord
Say hello to my little friend
Male User Gallery


Registered: 09/15/12
Posts: 37,810
Loc: Canada Flag
Trusted Cultivator
Re: My intro... [Re: dextr0]
    #23521648 - 08/08/16 10:38 AM (7 years, 5 months ago)

That is bacteria and mycelium in one nasty soup. You might be able to rescue some of the mycelium with an agar sandwich but I still can't say what species it is.


Extras: Filter Print Post Top
Offlinedextr0
Stranger
 User Gallery

Registered: 07/24/16
Posts: 431
Last seen: 20 days, 6 hours
Re: My intro... [Re: Pastywhyte]
    #23521666 - 08/08/16 10:48 AM (7 years, 5 months ago)

Thanx Pasty. So I'm going for the white puffs as opposed to flat milky stuff?? I didn't put up pic of the flat milky because it's hard to see unless I open jar.


Extras: Filter Print Post Top
InvisiblePastywhyteMDiscord
Say hello to my little friend
Male User Gallery


Registered: 09/15/12
Posts: 37,810
Loc: Canada Flag
Trusted Cultivator
Re: My intro... [Re: dextr0]
    #23521673 - 08/08/16 10:51 AM (7 years, 5 months ago)

The fuzzy stuff is mycelium. The milky shit is bacteria.


Extras: Filter Print Post Top
Offlinedextr0
Stranger
 User Gallery

Registered: 07/24/16
Posts: 431
Last seen: 20 days, 6 hours
Re: My intro... [Re: Pastywhyte]
    #23528407 - 08/10/16 02:30 PM (7 years, 5 months ago)

Lol. I isolated some green mold. Oh well. Getting in some practice counts too.


Extras: Filter Print Post Top
Offlinedextr0
Stranger
 User Gallery

Registered: 07/24/16
Posts: 431
Last seen: 20 days, 6 hours
Re: My intro... [Re: dextr0]
    #23562858 - 08/21/16 12:12 PM (7 years, 5 months ago)

unknown strain found at River. posted in ID form, never got answer. This is the only thing that I've got not to have contaminants. This is around 12 15 days old.


Extras: Filter Print Post Top
Offlinedextr0
Stranger
 User Gallery

Registered: 07/24/16
Posts: 431
Last seen: 20 days, 6 hours
Re: My intro... [Re: dextr0]
    #23607847 - 09/03/16 12:07 PM (7 years, 4 months ago)

Ok, so I successfully isolated the dried mushroom. I actually had to move so the jars I made ended up being I'm some pretty warm temps. I'd say one hundred plus temperatures (they were in a truck for a week). Anyway I go to put some of the things up and happen to pick up one of the jars, lo and behold it's fully colonized with a beautiful white mycelia, no contaminants. I do not know how old the jar is or if it's from my first attempts or if it was later attempts when I finally got a SAB and pressure cooker. Im just glad I was able to isolate as I really like this particular strain? Not sure that's the correct word to use. Anyway once I get this to fruit maybe someone will be able to identify it for me. I asked the grower and no name.
I also finally got my spore print from fsre. Penis envy 6. Fsre is bad ass. Ill for sure be sending prints so others can get the same blessing I have. I've been practicing taking prints with Parasols Lactea that have been popping up here lately.
I'd like to thank everyone who has stopped by and givin me pointers or insight so far. Y'all have been a big help.
The following pics are of the isolated mycelia and the practice prints. Lots of more pics to go as things progress. Hope y'all enjoy.
Again feel free to comment or ask any questions.


Extras: Filter Print Post Top
Offlinedextr0
Stranger
 User Gallery

Registered: 07/24/16
Posts: 431
Last seen: 20 days, 6 hours
Re: My intro... [Re: dextr0]
    #23607931 - 09/03/16 12:37 PM (7 years, 4 months ago)




Extras: Filter Print Post Top
Offlinedextr0
Stranger
 User Gallery

Registered: 07/24/16
Posts: 431
Last seen: 20 days, 6 hours
Re: My intro... [Re: dextr0]
    #23617152 - 09/06/16 06:41 AM (7 years, 4 months ago)

” To inoculate BRF with agar you don't have to drop a wedge and use GE lid, you can sterilize a syringe of water, squirt some water onto your culture and then agitate with the flame sterilized needle and suck the myc water up for a fast LI"

Does anyone not agree with this??

I want to try pastys straw tek with this.


Extras: Filter Print Post Top
Offlinedextr0
Stranger
 User Gallery

Registered: 07/24/16
Posts: 431
Last seen: 20 days, 6 hours
Re: My intro... [Re: dextr0]
    #23617300 - 09/06/16 07:41 AM (7 years, 4 months ago)

I also need to know a good brand for grass seed. Anyone wanna help?


Edited by dextr0 (09/06/16 07:46 AM)


Extras: Filter Print Post Top
Offlinedextr0
Stranger
 User Gallery

Registered: 07/24/16
Posts: 431
Last seen: 20 days, 6 hours
Re: My intro... [Re: dextr0]
    #23618782 - 09/06/16 04:06 PM (7 years, 4 months ago)

Ok...well since I got no reply I went and bought Millet. I was (or maybe still will if I get some nay's to my question) going to do Kramer cakes. But I didn't have enough money for vermiculite...
So....my question is can I invitro with just plain millet? I only need to get one mushroom from the clone so I can make a spore print. Be nice if I can get some knowledgeable help here. Things would go alot faster. If not I'll continue killing it anyway... Lol.


Extras: Filter Print Post Top
InvisiblemushboyMDiscord
modboy
 User Gallery


Registered: 04/24/05
Posts: 32,281
Loc: where?
Trusted Cultivator
OG Cultivator
Re: My intro... [Re: dextr0]
    #23619023 - 09/06/16 05:23 PM (7 years, 4 months ago)

verm is like 8 bucks.
if you cut a corner or skip a step you will fail.


Extras: Filter Print Post Top
Offlinedextr0
Stranger
 User Gallery

Registered: 07/24/16
Posts: 431
Last seen: 20 days, 6 hours
Re: My intro... [Re: mushboy]
    #23619061 - 09/06/16 05:37 PM (7 years, 4 months ago)

Thanx for the reply mush...u right it's $10. The millet was $3 for one pound...they are taxing over here. But OK, I'll make something happen.


Extras: Filter Print Post Top
OfflinecronicrFacebook
 User Gallery


Registered: 08/07/11
Posts: 61,436
Loc: Van Isle Flag
Last seen: 2 years, 9 days
Trusted Cultivator
Re: My intro... [Re: dextr0]
    #23619081 - 09/06/16 05:44 PM (7 years, 4 months ago)

Quote:

dextr0 said:
Thanx for the reply mush...u right it's $10. The millet was $3 for one pound...they are taxing over here. But OK, I'll make something happen.



Holy fuck that's expensive


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


Extras: Filter Print Post Top
Offlinespacechildo
proletarians rise up
Male User Gallery

Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
Re: My intro... [Re: dextr0]
    #23619122 - 09/06/16 05:56 PM (7 years, 4 months ago)

Quote:

dextr0 said:
Thanx for the reply mush...u right it's $10. The millet was $3 for one pound...they are taxing over here. But OK, I'll make something happen.




man, you just gotta find a different branch of stores, if you go to a health-food store its expensive as fuck,
look for animal feed, no farmer is gonna pay 3 or 10$ a pound for his horse food, it'd ruin him.


Extras: Filter Print Post Top
Offlinedextr0
Stranger
 User Gallery

Registered: 07/24/16
Posts: 431
Last seen: 20 days, 6 hours
Re: My intro... [Re: spacechildo]
    #23628877 - 09/09/16 12:15 PM (7 years, 4 months ago)

100 ml hulled millet
50ml water
Put into PP5 mini round
PC for 70 min

Will be adding a wedge of colonized agar tomorrow.


Edited by dextr0 (09/09/16 12:18 PM)


Extras: Filter Print Post Top
Offlinedextr0
Stranger
 User Gallery

Registered: 07/24/16
Posts: 431
Last seen: 20 days, 6 hours
Re: My intro... [Re: dextr0]
    #23658612 - 09/19/16 12:18 PM (7 years, 4 months ago)

one of the pp5. one got black mold...this is the other. no contaminants. I don't know if the black mold was because of prep or if I just haven't been sterile enough. I haven't gotten any gloves. no $.
This is colonized hulled millet by itself. I'll be experimenting more with hulled millet but later on. I will be using wbs as soon as I get $ together as its been proven with lots of teks I can follow. Temps have been in lower 60s to 70 degrees.


Edited by dextr0 (09/19/16 02:25 PM)


Extras: Filter Print Post Top
Offlinedextr0
Stranger
 User Gallery

Registered: 07/24/16
Posts: 431
Last seen: 20 days, 6 hours
Re: My intro... [Re: dextr0]
    #23671059 - 09/23/16 01:10 PM (7 years, 4 months ago)

I used that pp 5 and spawned to coir. The pic is 38 hrs after. Just plain coir heat treated with 180 degree water for bout an hour. Let cool then mixed spawn in. This is the isolate.

I also pced some more hulled millet (50 min., did it twice to same batch). this time I did not measure used about a third of water instead of half the amount of water. big pp 5 was nocked up with Penns Envy 6 ms. Small is the isolet.

Other pic is just some ropes mycelia that I thought looked cool.

last pic is the original agar I made which all other mycelia was taken from (isolate). it's all dried up. I transferred it to fresh agar yesterday.





Edited by dextr0 (09/23/16 01:14 PM)


Extras: Filter Print Post Top
Offlinedextr0
Stranger
 User Gallery

Registered: 07/24/16
Posts: 431
Last seen: 20 days, 6 hours
Re: My intro... [Re: dextr0]
    #23671077 - 09/23/16 01:18 PM (7 years, 4 months ago)

Just a by the by...I'm about $200+ into this. Shit not cheap but I applied myself. This is coming from someone who lives on maybe $50 a month, if I'm lucky.


Extras: Filter Print Post Top
Offlinedextr0
Stranger
 User Gallery

Registered: 07/24/16
Posts: 431
Last seen: 20 days, 6 hours
Re: My intro... [Re: dextr0]
    #23677003 - 09/25/16 02:51 PM (7 years, 4 months ago)

I just sent in for some Redboy from FSRE. I want this one pretty bad. Alternatively I asked for a race called Cambodia Gold. Looked intresting:
Cambodian Gold
The Cambodian Gold is a F1 hybrid of Psilocybe Golden Teacher x Cambodia. This strain is developed by a dutch Growkit producer to immediately start growing a variety of the iconic Golden Teacher strain at home.
It has long been a dream for this producer to provide an easy-to-use grow kit for Psilocybe cubensis Golden Teacher, but the strain underperformed and seemed unsuitable as a ‘Ready-To-Grow’ box. However, through a process of controlled mycelium growing and careful selections, the spores of Cambodia and Golden Teacher were brought together as a powerful cross with hybrid vigour that is called “Cambodian Gold”.
The Cambodian Gold was first introduced as a ‘Ready-To-Grow’ box at Azarius!


Edited by dextr0 (09/25/16 02:52 PM)


Extras: Filter Print Post Top
Offlinemrmazdarx9
Pffffttt


Registered: 05/15/16
Posts: 9,796
Loc: behind you
Last seen: 1 year, 4 months
Re: My intro... [Re: dextr0]
    #23677194 - 09/25/16 04:06 PM (7 years, 4 months ago)

Don't buy a grow kit, I can't see how it would be any difference to regular GT. The description makes no sense "This strain is developed by a dutch Growkit producer to immediately start growing a variety of the iconic Golden Teacher strain at home" huh??? Just grow the apparently iconic GT at home? The B.S. companies come up with to sell spores :rofl:


--------------------
COCA GROWERS come here and share your knowledge
COCA GROWERS UNITE
:penis:Click here for UK trades:penis:
:penis: need some supplies in the UK check Here or PM me:penis:
UK OTD uk members chat
UK supplies and trade OTD place to chat shit
Right Here
If you use "SWIM" you should DROWN


Extras: Filter Print Post Top
Offlinedextr0
Stranger
 User Gallery

Registered: 07/24/16
Posts: 431
Last seen: 20 days, 6 hours
Re: My intro... [Re: mrmazdarx9]
    #23677261 - 09/25/16 04:29 PM (7 years, 4 months ago)

I don't have the money for a grow kit. Besides, im a DIY kind of person if u couldn't tell yet. I bought a spore print is what I'm saying. Thanks for looking out tho man.
FSRE=free spore ring earth...$2 spore prints. I just send $3
http://fsre.nl/


Edited by dextr0 (09/25/16 04:31 PM)


Extras: Filter Print Post Top
Offlinemrmazdarx9
Pffffttt


Registered: 05/15/16
Posts: 9,796
Loc: behind you
Last seen: 1 year, 4 months
Re: My intro... [Re: dextr0]
    #23677264 - 09/25/16 04:30 PM (7 years, 4 months ago)

Good stuff mate:highfive:


--------------------
COCA GROWERS come here and share your knowledge
COCA GROWERS UNITE
:penis:Click here for UK trades:penis:
:penis: need some supplies in the UK check Here or PM me:penis:
UK OTD uk members chat
UK supplies and trade OTD place to chat shit
Right Here
If you use "SWIM" you should DROWN


Extras: Filter Print Post Top
Offlinedextr0
Stranger
 User Gallery

Registered: 07/24/16
Posts: 431
Last seen: 20 days, 6 hours
Re: My intro... [Re: mrmazdarx9]
    #23685733 - 09/28/16 08:11 AM (7 years, 4 months ago)

Am I correct to assume that since I spawned to coir with very little grain...my flushes will be small??
And if so what can I do to get a better flush this time?


Extras: Filter Print Post Top
Offlinedextr0
Stranger
 User Gallery

Registered: 07/24/16
Posts: 431
Last seen: 20 days, 6 hours
Re: My intro... [Re: dextr0]
    #23744309 - 10/17/16 12:50 AM (7 years, 3 months ago)

Two little pins on two pasty pods of PE6 got me breakdancing and shit over here...I'll post pics tomorrow. Fucking trich in almost all my tubs, grains, etc. Had me feeling blue.


Extras: Filter Print Post Top
Offlinedextr0
Stranger
 User Gallery

Registered: 07/24/16
Posts: 431
Last seen: 20 days, 6 hours
Re: My intro... [Re: dextr0]
    #23748202 - 10/18/16 08:25 AM (7 years, 3 months ago)

OK so update. Took a couple days because I'm a DXM head. Old school too, so shit takes its toll now.
Anyway pins. One grew half an inch in one night. Also have a picture of some agar that's dried up. I'm actually wondering if anyone else has dealt with this.  It's got the oldest mycelia of all agar plates and every time I transfer it soaks the shit dry fast. I'm wondering why.
Jar, it's a gallon?  Maybe half gallon...I inoculated with a very small amount of spawn via g2g. Taking kind of long to fully colonize. I think. 16 days so far.
Ariel mycelia I believe. Just thought it looked interesting. Looks like its trying to run away from the contamination.







Extras: Filter Print Post Top
OfflinePortabellaFella 1
Enthusiastic
Male User Gallery


Registered: 08/08/16
Posts: 654
Last seen: 2 years, 9 months
Re: My intro... [Re: dextr0]
    #23748212 - 10/18/16 08:29 AM (7 years, 3 months ago)

A gallon is huge for grain. You'll probably be overrun by contamination before the myc gets to 100%.
A smaller jar, fully colonized then transferred to other jars is the way to go.
Not saying a gallon won't work but time is an enemy.

EDIT:Never mind.... the last pic already shows green mold.... toss asap and never op n your colonizing grain jars
EDIT#2: Nevermind... you already knew that lol


--------------------
I would like to acquire anything I don’t have


Edited by PortabellaFella 1 (10/18/16 08:32 AM)


Extras: Filter Print Post Top
InvisiblePastywhyteMDiscord
Say hello to my little friend
Male User Gallery


Registered: 09/15/12
Posts: 37,810
Loc: Canada Flag
Trusted Cultivator
Re: My intro... [Re: dextr0]
    #23748213 - 10/18/16 08:29 AM (7 years, 3 months ago)

Pins suck the moisture out of the plates as they grow. If the culture was ms you should transfer the pins to make for really clean clones.

Those wbs jars are far too full. You are not going to be able to shake them easily.


Extras: Filter Print Post Top
Offlinedextr0
Stranger
 User Gallery

Registered: 07/24/16
Posts: 431
Last seen: 20 days, 6 hours
Re: My intro... [Re: Pastywhyte]
    #23748324 - 10/18/16 09:12 AM (7 years, 3 months ago)

Sup fellas thanx for stopping in. I already know I messed up using the big jar. I do stuff on a whim alot because I'm a bit analytical and tend to over think things. I'll read and read and as you know there are plenty of different ways to do one thing. Eventually I choose one and usually use a little from here little from there... Anyway yea I realize I messed up putting in to much wbs in jar...its been shaken once about 20% colonization, I know I should have waited for 30% but because the jar was bigger I shook to spread the mycelia in hopes that it would colonize faster from more points since it was just colonizing the top at first. I will b filling to 75% of jar from now on if i have to I'll spoon it out with a sterile spoon.
Pasty, how can I get my agar to stop pinning?? It looks like my two PE6 jars are going to keep pinning. They are MS from print. I'm already onto cloning the pins because they are pretty clean and from what I understand are better choices for strong growth. The biggest pin im going to let grow in vivo tho. I want a clean print.


Edited by dextr0 (10/18/16 09:16 AM)


Extras: Filter Print Post Top
InvisiblePastywhyteMDiscord
Say hello to my little friend
Male User Gallery


Registered: 09/15/12
Posts: 37,810
Loc: Canada Flag
Trusted Cultivator
Re: My intro... [Re: dextr0]
    #23748344 - 10/18/16 09:20 AM (7 years, 3 months ago)

You don't want to stop the agar from pinning, those pins are going to be good clones. People look for those. Clone them pins and then drop the wedges to grain before they have a chance to pin.


Extras: Filter Print Post Top
Offlinedextr0
Stranger
 User Gallery

Registered: 07/24/16
Posts: 431
Last seen: 20 days, 6 hours
Re: My intro... [Re: Pastywhyte]
    #23748842 - 10/18/16 12:45 PM (7 years, 3 months ago)

Thanks pasty. I can't help but feel that if I let the biggest pin continue i will be doing myself a disservice. I say this because I feel like If i let it sporulate it's adding more genes to the mix. I don't know it just feels backwards. Might be wrong.


Edited by dextr0 (10/18/16 12:50 PM)


Extras: Filter Print Post Top
Offlinemrmazdarx9
Pffffttt


Registered: 05/15/16
Posts: 9,796
Loc: behind you
Last seen: 1 year, 4 months
Re: My intro... [Re: dextr0]
    #23748854 - 10/18/16 12:51 PM (7 years, 3 months ago)

That's why you clone it so it doesn't have loads of variations in the mix.


--------------------
COCA GROWERS come here and share your knowledge
COCA GROWERS UNITE
:penis:Click here for UK trades:penis:
:penis: need some supplies in the UK check Here or PM me:penis:
UK OTD uk members chat
UK supplies and trade OTD place to chat shit
Right Here
If you use "SWIM" you should DROWN


Extras: Filter Print Post Top
Offlinedextr0
Stranger
 User Gallery

Registered: 07/24/16
Posts: 431
Last seen: 20 days, 6 hours
Re: My intro... [Re: mrmazdarx9]
    #23751789 - 10/19/16 11:50 AM (7 years, 3 months ago)

Update:
I took three pins, transfered to new agar.
Let biggest pin go it sporulated early this morning. I took the fruit cut a piece from center to clone, cut the cap off and am making a print.
Since they seem to like to pin I threw one onto a plastic pod of agar with poms cut up into peices.  Figure it will be easier to pull individual pieces of pom or pins w poms already pre cut.
Two others went to plain agar pods.
The fruit clone was transfered to agar also. It has a root in it going all the way across one side in agar the other not. It's diagnal. It's for easy transfer, I saw a post about using toothpicks to do this. I used a root from Desmanthus illinoensis that I've had drying for a couple months now. It was pced along with everything else for 90 min. Im just experimenting.
One thing that was really curious to me was the fully formed fruit did not bruise when cut. One of my pins did, a very Navy blue. Almost black ish. The agar plates that pinned did to tho a lightish aqua blue.

Just some observations, etc. I'll post pics later. Gotta get a good phone.


Extras: Filter Print Post Top
Offlinedextr0
Stranger
 User Gallery

Registered: 07/24/16
Posts: 431
Last seen: 20 days, 6 hours
Re: My intro... [Re: dextr0]
    #23767930 - 10/24/16 07:16 PM (7 years, 3 months ago)


1. Agar drying from oldest mycellia. Two almost three months old. Transfered to new agar a few times in between. No pinning at all. Very thin growth. Only one transfer grew ropey.
2. Ropey growth of same variety.
3. Colonized side of jar. Taking too long to colonize. Any tips would b appreciated. I kind of feel I may have stretched this too far. This mycellia comes from three month old mycellia. Transfered countless times and then stretched further by me using maybe a tablespoon of grain to inoculate the half gallon jar. Want to use as spawn to coir but am afraid it doesn't have the strength to do that. Which makes me just wanna case the grain. Thoughts??
4. Un colonized side of jar.
5. Another jar. Contamination city.


Edited by dextr0 (10/24/16 07:33 PM)


Extras: Filter Print Post Top
Offlinemrmazdarx9
Pffffttt


Registered: 05/15/16
Posts: 9,796
Loc: behind you Flag
Last seen: 1 year, 4 months
Re: My intro... [Re: dextr0]
    #23768856 - 10/25/16 02:19 AM (7 years, 3 months ago)

You shouldnt fill your jars that full how you gonna shake them up


--------------------
COCA GROWERS come here and share your knowledge
COCA GROWERS UNITE
:penis:Click here for UK trades:penis:
:penis: need some supplies in the UK check Here or PM me:penis:
UK OTD uk members chat
UK supplies and trade OTD place to chat shit
Right Here
If you use "SWIM" you should DROWN


Extras: Filter Print Post Top
Offlinedextr0
Stranger
 User Gallery

Registered: 07/24/16
Posts: 431
Last seen: 20 days, 6 hours
Re: My intro... [Re: mrmazdarx9]
    #23769033 - 10/25/16 05:54 AM (7 years, 3 months ago)

I know man those jars were all made at around the same time. I'm learning. The wbs expanded way more than I thought it would. On top of that I was in a real hurry when I made bottom jar. It was actually a last min thing. I left my wbs soaking for two days and went back to strain so it wouldnt ferment. Strained, then had to leave. So I jarred some up. Came back two days later to find the seeds had popped and I had all kinds of growth. I also had threw in a large chunk of colonized agar while on dxm. Shrug....


Extras: Filter Print Post Top
Offlinedextr0
Stranger
 User Gallery

Registered: 07/24/16
Posts: 431
Last seen: 20 days, 6 hours
Re: My intro... [Re: dextr0]
    #23776865 - 10/27/16 02:04 PM (7 years, 3 months ago)

Everything got green. Not sure what this is but I got green on everything but what was in glass. Also the agar with the stick in it, it surprisingly is clean also. The pods turned spotted with mycellia that quickly turned green. Some green then black. One turned completely green in two days! The whole bottom was yellow too. Strange.
I have some new plastics I found at dollar tree (USA), pcing now see if they hold up.
I will be starting all over. I have a master of the unknown variety for later work. For now I will attempt to get some clean cultures and start some new cultures from spore. PE6 again sense they seem to want to grow so badly.
Also any day I should be getting another print.


Extras: Filter Print Post Top
Offlinedextr0
Stranger
 User Gallery

Registered: 07/24/16
Posts: 431
Last seen: 20 days, 6 hours
Re: My intro... [Re: dextr0]
    #23777542 - 10/27/16 05:23 PM (7 years, 3 months ago)

I got some straw from Walmart. It has some black mold spots. Its all dry but I'm wondering if its OK to use. I wanted to do the bucket teck for pasteurization but idk about that with the mold.
Oh and the dollar tree plastics held up. 10 for $1...or 5 of the reds for same price.


Extras: Filter Print Post Top
Offlinedextr0
Stranger
 User Gallery

Registered: 07/24/16
Posts: 431
Last seen: 20 days, 6 hours
Re: My intro... [Re: dextr0]
    #23781326 - 10/28/16 09:26 PM (7 years, 3 months ago)


1. PE6 from pin. Pom agar.
2. Unknown variety (cubensis). Just cool pic sideways tho.
3-5. Contamination?? Anyone have any idea what kind?? I tried get good shots.


Extras: Filter Print Post Top
Offlinedextr0
Stranger
 User Gallery

Registered: 07/24/16
Posts: 431
Last seen: 20 days, 6 hours
Re: My intro... [Re: dextr0]
    #23785560 - 10/30/16 01:52 PM (7 years, 2 months ago)



Scrubbers or POM's that are good. Didn't even rinse em off just poured agar on top and pced. Growth started the next day. Clean, has been so far anyway. Brand is scrub buddies from dollar tree (USA). I'm posting because I know some have had trouble getting workable POM's. (Pasty, if u still ain't got a source u do now) Figured I would help by giving good brand and location to find.
Also got some syringes. 20 gage, but I'm sure I can make em work.


Extras: Filter Print Post Top
Offlinedextr0
Stranger
 User Gallery

Registered: 07/24/16
Posts: 431
Last seen: 20 days, 6 hours
Re: My intro... [Re: dextr0]
    #23794839 - 11/02/16 03:06 PM (7 years, 2 months ago)

I keep getting the milky bacteria on my agar no matter how sterile I am. I took care of the green mold by cleaning up the area and throwing anything contaminated away. I need some pointers if someone wouldn't mind sharing.

Agar recipe:
3/4 tbs agar
1 tbs potato flakes
1 drop of honey
125 mil water

I also have a cheap 6 qt PC. Its a rocker,tDenmark brand. I PC from 45 min to 60 min. Slow rock because if u turn down slightly u get no rock at all.


Extras: Filter Print Post Top
InvisibleMycolorado
Hobbyist
Male User Gallery

Registered: 07/23/16
Posts: 8,529
Loc: Interdimensional Bootcamp
Trusted Cultivator
Re: My intro... [Re: dextr0]
    #23795098 - 11/02/16 04:28 PM (7 years, 2 months ago)

Hey dextr0, you should weigh the dry ingredients as opposed to measuring them and might consider some petri dishes...they're cheap and make plate work much easier.  PC your agar for 30 minutes at 15psi and pour in a SAB.  You should be good to go. 

Standard 2% nutrient medium recipe (you can make whatever volume you want):

1000ml H2O
20g agar
20g nutrient (malt extract, potato flake, dog food)


Edited by Mycolorado (11/02/16 04:30 PM)


Extras: Filter Print Post Top
Offlinespacechildo
proletarians rise up
Male User Gallery

Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
Re: My intro... [Re: Mycolorado]
    #23795118 - 11/02/16 04:36 PM (7 years, 2 months ago)

his recipe sounds good, bacteria comes from poor sterile tech or really fucked syringes.

its all about practice, smooth movements, keep air still, dont hover hands above open media.
and if you see clean growth take a transfer quick and dont let bacteria run over your plates.


Extras: Filter Print Post Top
Offlinedextr0
Stranger
 User Gallery

Registered: 07/24/16
Posts: 431
Last seen: 20 days, 6 hours
Re: My intro... [Re: Mycolorado]
    #23795150 - 11/02/16 04:50 PM (7 years, 2 months ago)

Yea. Been meaning to do that since I started agar. Store was out of preferred scale so I basically tried to just feel it out and keep it comfortable. I try to keep it as close to pasty math as much as possible tho. U really think that's my problem? Either way yea I need a scale so I'm get one.
I really wonder if my PC even reaches 15psi.
Thanks for helping.


Extras: Filter Print Post Top
InvisibleMycolorado
Hobbyist
Male User Gallery

Registered: 07/23/16
Posts: 8,529
Loc: Interdimensional Bootcamp
Trusted Cultivator
Re: My intro... [Re: spacechildo]
    #23795152 - 11/02/16 04:50 PM (7 years, 2 months ago)

Fosho...just thought I'd put up the standard mix as it's easy to scale. 

dextr0,
You might pour a plate and set it to the side (don't transfer anything to it)to see if anything grows on it...will help narrow down the problem.  If something grows, you know it's contaminating when you pour and not when you transfer or that there is an issue with the prep...

Edit
If your PC doesn't hit 15psi just let it run for 45-hour.


Edited by Mycolorado (11/02/16 04:52 PM)


Extras: Filter Print Post Top
Offlinedextr0
Stranger
 User Gallery

Registered: 07/24/16
Posts: 431
Last seen: 20 days, 6 hours
Re: My intro... [Re: Mycolorado]
    #23795352 - 11/02/16 05:52 PM (7 years, 2 months ago)

Thanks fellas. I guess I just need to work on smooth movements. I'm not using syringes at all at this point. Have some but haven't used. I'm stuck on getting a clean plate, which I can't seem to get. Im honestly just trien to actually pay attention to the agar growing over contams( i mean not trying to get those pieces). Ive been under the assumption that if I had clean looking plate the mushies won. Little did I know they ride along. So I'm dealing with a bunch of dirty cultures. And constant change of environment from moving a lot. I clean up as best I can but u know...
I'm going to try to transfer a piece of agar to another agar. See if its my sterile tek. I have some premade agar that seems pretty clean. No milky look. Anyway I just add to plates or pods or jars and PC.
Ive been planing just not sure bout some things.


Edited by dextr0 (11/02/16 05:56 PM)


Extras: Filter Print Post Top
Offlinedextr0
Stranger
 User Gallery

Registered: 07/24/16
Posts: 431
Last seen: 20 days, 6 hours
Re: My intro... [Re: dextr0]
    #23806328 - 11/06/16 12:04 PM (7 years, 2 months ago)



This is the half gallon that never fully colonized spawned to unpasteurised straw. I just wanted it to work so bad cause its the last of cube var. The other plates got contams. I don't even know if its a fruiting culture. I've had some jars of agar for months now and zero pinning. It did come from a fruit tho.


Edited by dextr0 (11/06/16 04:24 PM)


Extras: Filter Print Post Top
Offlinedextr0
Stranger
 User Gallery

Registered: 07/24/16
Posts: 431
Last seen: 20 days, 6 hours
Re: My intro... [Re: dextr0]
    #23807493 - 11/06/16 05:31 PM (7 years, 2 months ago)

How does this look?? 11 days.


Extras: Filter Print Post Top
Offlinedextr0
Stranger
 User Gallery

Registered: 07/24/16
Posts: 431
Last seen: 20 days, 6 hours
Re: My intro... [Re: dextr0]
    #23821262 - 11/10/16 09:42 PM (7 years, 2 months ago)

I've got a question. If I use a bread tie as a loop how long do I need keep lighter on on metal to insure sterilization?? Or the flame I mean.
This is the last thing I'm going to check before I just go for a new PC. The one I have is small af anyway and leaks water n shit.  Was brand new but cheaply.


Extras: Filter Print Post Top
Offlinedextr0
Stranger
 User Gallery

Registered: 07/24/16
Posts: 431
Last seen: 20 days, 6 hours
Re: My intro... [Re: dextr0]
    #23825050 - 11/12/16 06:59 AM (7 years, 2 months ago)






This is five days growth. Very slow if u ask me. So I'm wondering should I case now?? Should I wait it out? Should I take off all exposed straw give a couple days to recover, then case?
Help me fuckers.


Extras: Filter Print Post Top
InvisibleMycolorado
Hobbyist
Male User Gallery

Registered: 07/23/16
Posts: 8,529
Loc: Interdimensional Bootcamp
Trusted Cultivator
Re: My intro... [Re: dextr0]
    #23825080 - 11/12/16 07:22 AM (7 years, 2 months ago)

Best thing to do at this point is close it up and come back in 5 days.  Also looks like it could have been pasteurized a bit better...there are some seeds germinating in there...what was that process?


Extras: Filter Print Post Top
Offlinedextr0
Stranger
 User Gallery

Registered: 07/24/16
Posts: 431
Last seen: 20 days, 6 hours
Re: My intro... [Re: Mycolorado]
    #23825131 - 11/12/16 07:58 AM (7 years, 2 months ago)

Hey Mycolorado thanks for stopping in.
You see that's the whole problem. The spawn I used was sterilized, but I'm sure was bacterial as it never fully colonized (waited almost a month). I did use it no matter what. Just had a hunch it might work.
The straw I used though was never pasteurized or sterilized. I kno I shouldn't have even tried but I had to.

I'm fine if nothing comes out of this, but I need some assistance to maybe pull it off. I just need one print to play with.
The two pictures above were a comparison of what's going on. Same tub 5 days apart.
The tub is going on three plus weeks.


Extras: Filter Print Post Top
InvisiblePastywhyteMDiscord
Say hello to my little friend
Male User Gallery


Registered: 09/15/12
Posts: 37,810
Loc: Canada
Trusted Cultivator
Re: My intro... [Re: dextr0]
    #23825338 - 11/12/16 09:21 AM (7 years, 2 months ago)

With uncolonized grain in there it's toast before it began. But if it was to have a fighting chance, straw needs to be cut fine, 1" to 2" max. Finer is better. Straight straw generates a lot of heat. It's better to have it mixed with some coir or manure or both.


Extras: Filter Print Post Top
Offlinedextr0
Stranger
 User Gallery

Registered: 07/24/16
Posts: 431
Last seen: 20 days, 6 hours
Re: My intro... [Re: Pastywhyte]
    #23827285 - 11/12/16 09:05 PM (7 years, 2 months ago)

Thanx Pasty, u mean for next time right??

This is my biggest fear. That the casing will introduce more harmefull bacteria or mold and it ends all chances. Same if I was to add anything to it or add it to anything.


Extras: Filter Print Post Top
InvisiblePastywhyteMDiscord
Say hello to my little friend
Male User Gallery


Registered: 09/15/12
Posts: 37,810
Loc: Canada
Trusted Cultivator
Re: My intro... [Re: dextr0]
    #23827402 - 11/12/16 09:39 PM (7 years, 2 months ago)

Yeah I mean for next time. This time is pretty much fucked.


Extras: Filter Print Post Top
Offlinedextr0
Stranger
 User Gallery

Registered: 07/24/16
Posts: 431
Last seen: 20 days, 6 hours
Re: My intro... [Re: dextr0]
    #23827449 - 11/12/16 09:59 PM (7 years, 2 months ago)

Lol. Thanx.
Nice surprise just now. It looks clean. Pe6. I wonder if its truly clean though. Would b nice to have something for Christmas. I'm just looking for a clean culture. To do this shit right.

I did try to make a transfer like a week ago...but I thought it had mycogone. It had a lot of condensation on sides and like 3 dots on mycelium... But those seem to have dried up along with the condensation. The transfer I think ended up bacterial so I threw it this one to the side for a little while. I should probably try another transfer.


Extras: Filter Print Post Top
InvisibleMycolorado
Hobbyist
Male User Gallery

Registered: 07/23/16
Posts: 8,529
Loc: Interdimensional Bootcamp
Trusted Cultivator
Re: My intro... [Re: dextr0]
    #23828378 - 11/13/16 09:23 AM (7 years, 2 months ago)

What's that growing on?


Extras: Filter Print Post Top
Offlinedextr0
Stranger
 User Gallery

Registered: 07/24/16
Posts: 431
Last seen: 20 days, 6 hours
Re: My intro... [Re: Mycolorado]
    #23828573 - 11/13/16 10:20 AM (7 years, 2 months ago)

Hey Myc.
Its on a pan scrubber. Idea is from here https://www.shroomery.org/forums/showflat.php/Number/20643482
I'm trying to find a way to make this work with a modified grocery store agar. Admittedly I need to go over the tek again. A few things I'm not sure of yet.


Extras: Filter Print Post Top
InvisibleMycolorado
Hobbyist
Male User Gallery

Registered: 07/23/16
Posts: 8,529
Loc: Interdimensional Bootcamp
Trusted Cultivator
Re: My intro... [Re: dextr0]
    #23830281 - 11/13/16 07:44 PM (7 years, 2 months ago)

Ha, that's what I assumed it was but wasn't sure.  Crazy!


Extras: Filter Print Post Top
Offlinedextr0
Stranger
 User Gallery

Registered: 07/24/16
Posts: 431
Last seen: 20 days, 6 hours
Re: My intro... [Re: Mycolorado]
    #23830352 - 11/13/16 08:03 PM (7 years, 2 months ago)

I like the idea of short time for pinning so I can transfer to agar.15 days and no pins. But I'm using regular grocery store tek agar. I mean that's where I thought I would start.
I need to read the tek again. Thing is I like to read a whole thread. Its a long thread. And I get side tracked along the way checking out threads mentioned along the way. Yes I'm that guy.
I forgot exactly why I was gonna mention this but the pins take average two three days to show growth. Lol.
Its 420 round here.


Extras: Filter Print Post Top
Offlinedextr0
Stranger
 User Gallery

Registered: 07/24/16
Posts: 431
Last seen: 20 days, 6 hours
Re: My intro... [Re: dextr0]
    #23837275 - 11/15/16 11:05 PM (7 years, 2 months ago)

About the morphology of colonies
https://eurekabrewing.wordpress.com/2013/03/


Extras: Filter Print Post Top
Offlinedextr0
Stranger
 User Gallery

Registered: 07/24/16
Posts: 431
Last seen: 20 days, 6 hours
Re: My intro... [Re: dextr0]
    #23838827 - 11/16/16 01:28 PM (7 years, 2 months ago)

Update: don't know if this is brusing or something else. Better pics in a bit. I constantly squeeze the sides for GE. So maybe maybe not. Any opinions??


Extras: Filter Print Post Top
Offlinedextr0
Stranger
 User Gallery

Registered: 07/24/16
Posts: 431
Last seen: 20 days, 6 hours
Re: My intro... [Re: dextr0]
    #23842004 - 11/17/16 02:34 PM (7 years, 2 months ago)




Extras: Filter Print Post Top
InvisiblePastywhyteMDiscord
Say hello to my little friend
Male User Gallery


Registered: 09/15/12
Posts: 37,810
Loc: Canada Flag
Trusted Cultivator
Re: My intro... [Re: dextr0]
    #23842016 - 11/17/16 02:38 PM (7 years, 2 months ago)

That ain't looking happy at all


Extras: Filter Print Post Top
Offlinedextr0
Stranger
 User Gallery

Registered: 07/24/16
Posts: 431
Last seen: 20 days, 6 hours
Re: My intro... [Re: Pastywhyte]
    #23842121 - 11/17/16 03:05 PM (7 years, 2 months ago)

Yes I know. Its ok. I'm gonna start some more spawn soon. Do things right. Finally got a clean plate of the PE6.
While I'm doing that grow I'm going to clean this culture up and give it another go when I do. I have a few grains left over on the bottom of a jar.
I have another pic of the blue to add. Gotta charge phone. I also let the lid off in a makeshift SAB to give it some good GE and get some of that moisture out of the box. I secretly hope it initiates pinning.

I'm also checking out the potato dextrose broth. I recently saw a post saying it can be used to basicly grow a stronger mycelia when working with a old culture. This specific culture did have a very wispy light mycelia.

Quote:

Grundalizer said:
Any white mycelia, fluffy or not, which is derived from a known spore addition to a sterile media under sterile or semi-sterile conditions and is growing out of the area you doped the media with, is 99.99% what you are looking for.  Cobwebby growth does not equal contamination.  I'm a microbiologist and mycologist by trade and I'm amazed at how often I see people on hear freaking out about contamination and throwing things out (not trying to call you out, I just don't post much, and am trying to start to more).  If you have anything growing on a plate, just pick a corner with a sterile scalpel or sterile toothpick and re-plate...subculture till purity. 

Any moulds are easy to see because of their almost instantaneous rapid color changes due to conidia with spores forming which change color often due to their age.

Any true basidiomycete will stay in a white mycelial growth, and yes, often if I regenerate old spores or super old dried our desicated agar plates, initial growth is very very very wispy. 

I found sub-culturing doesn't help, but taking some and dropping it into sterile broth, like potatoe dextrose broth, yields very healthy and dense mycelial growth...which I then re-plate onto solid agar.



https://www.shroomery.org/forums/showflat.php/Number/23824055/fpart/1/vc/1/nt/2

Agar broth, throw out your crap LCs
https://www.shroomery.org/forums/showflat.php/Number/13781184/fpart/1/vc/1


Edited by dextr0 (11/17/16 03:23 PM)


Extras: Filter Print Post Top
InvisiblePastywhyteMDiscord
Say hello to my little friend
Male User Gallery


Registered: 09/15/12
Posts: 37,810
Loc: Canada Flag
Trusted Cultivator
Re: My intro... [Re: dextr0]
    #23842279 - 11/17/16 03:42 PM (7 years, 2 months ago)

I use LC all the time. Wedges to LC works great but lately I do this.

https://www.shroomery.org/forums/showflat.php/Number/23740857

Biggest issue with LC is the use of dirty inoculate  (spores) and the second biggest issue is the use of sugary media which creates sluggish growth.


Extras: Filter Print Post Top
Offlinedextr0
Stranger
 User Gallery

Registered: 07/24/16
Posts: 431
Last seen: 20 days, 6 hours
Re: My intro... [Re: Pastywhyte]
    #23855653 - 11/21/16 08:51 PM (7 years, 2 months ago)

Finally. Mission accomplished. Pins from the contaminated tub. This is all I wanted. I will now transfer to agar and clean up.
I was happy because I have plates that are old as hell. In fact this is the dry mushroom culture that I've been trying to regenerate for three months now. So I have a young culture to work with and I know it will fruit.. Which I was beginning to have doubts about. I was actually contemplating throwing it out today.


Edited by dextr0 (11/22/16 08:12 AM)


Extras: Filter Print Post Top
InvisibleMycolorado
Hobbyist
Male User Gallery

Registered: 07/23/16
Posts: 8,529
Loc: Interdimensional Bootcamp
Trusted Cultivator
Re: My intro... [Re: dextr0]
    #23856413 - 11/22/16 07:30 AM (7 years, 2 months ago)

Right on!  Your plan sounds good.  You should keep this thread going to illustrate how to go from a questionable grow to a nice clean pruductive one.


Extras: Filter Print Post Top
Offlinedextr0
Stranger
 User Gallery

Registered: 07/24/16
Posts: 431
Last seen: 20 days, 6 hours
Re: My intro... [Re: Mycolorado]
    #23857479 - 11/22/16 02:12 PM (7 years, 2 months ago)

This print came in from FSRE:
Teonanacatl
This strain was observed and collected for the 1st time by Mauritius (founder of the pro-fungi, Brasil) in the Lowland region Careiro, state of Amazonas, Brazil.
During the rainy season extending from September to May, buffalo breeders bring their pets to farms flooded by the road BR 319 that connects the Careiro Varzea Careiro to Brown. With the end of the rains the animals are transported to other areas. In July of 2008 Mauritius entered a farm and collected a mushroom of the genus Psilocibec cubensis from the buffalo manure.
The spores of this strain where clean on agar and sectors of mycelium where isolated for 1 year to select the best growing mycelium on rice and corn.
The name is in reference to the site Teonanacatl.

On a side note my Redboy never showed up. Guess I got jacked. *shrug*


Extras: Filter Print Post Top
Offlinedextr0
Stranger
 User Gallery

Registered: 07/24/16
Posts: 431
Last seen: 20 days, 6 hours
Re: My intro... [Re: dextr0]
    #23871788 - 11/27/16 11:41 AM (7 years, 2 months ago)

Here's a cool picture of some PE6 colonizing some Desmanthus Illinoensis roots. I will make a substrate containing small pieces mixed in as in experiment in the future.
I realize this has contams I'm just trying different things. Putting a small stick in with agar would make my transfers easier and I believe cleaner. This container sucked because it was not clear so I opened constantly. Like I said was experimenting only.

Anyway I've got some WBS ready for inoculation. Probably agar wedges to the grain. I'm kind of curious of how some sterile water into the dish with pom drawn up into syringe would work out. Thing is I'm not sure its clean as its hard to tell because of scrubby.


Extras: Filter Print Post Top
Offlinedextr0
Stranger
 User Gallery

Registered: 07/24/16
Posts: 431
Last seen: 20 days, 6 hours
Re: My intro... [Re: dextr0]
    #23989387 - 01/07/17 12:08 PM (7 years, 23 days ago)

OK so been busy being a whore...I've been doing lil things here and there though so new shit.
I for the life of me couldn't get a sterile plate... I've got two now but its pure luck I feel.


I did stop using bread tie inoculation loops. I want to try more but I happened to change to a syringe and have four clean transfers so far.
I made a couple vtek jars...one contaminated bad.



I also made another with corn meal instead and the POM's cut into 8 pieces so I can inoculate then use as spawn. I just haven't inoculated yet.

Talked a friend into growing. Who knos what he will do long term he's very lazy...he did go buy huge bags of verm, perlite, made a SAB, jars, and grinded out bunch of bfr. So I hooked him up with some spores (we made a syringe off the Teo from FSRE, drunk as fuck having no idea how to do it exactly but I got it done) and knocked up a few jars. One contaminated so I brought it home grabbed a few pieces of mycelia and am cleaning it on agar now.


A PE6 off agar.



All PE6 jars pinned like crazy.



Still can't get my WBS hydrated right. Tried a lot of different things but no go still so I'm working on that still.

Links I've been checking out interested in...maybe interest others:

Looking for sources of dextrose I ran into this...
The Experiments, vol. 1: Bee Pollen Granules and SpawnMate
https://www.shroomery.org/forums/showflat.php/Number/2301527

Brothing...
*NEW* Alien Broth Tek --- with pictures!
https://www.shroomery.org/forums/showflat.php/Number/1656605#1656605

Make sure to read the caption at bottom of pics here on this one.

"For PDY Broth: made the same way but omitting agar. For Acide PDYA: add 1 ml Lactic Acid to liquid media just before pouring plates."
http://plantpath.psu.edu/facilities/mushroom/cultures-spawn/media-preparation


Extras: Filter Print Post Top
InvisiblePastywhyteMDiscord
Say hello to my little friend
Male User Gallery


Registered: 09/15/12
Posts: 37,810
Loc: Canada Flag
Trusted Cultivator
Re: My intro... [Re: dextr0]
    #23989399 - 01/07/17 12:11 PM (7 years, 23 days ago)

Both of those plates have satellites but they could be easily cleaned up. There is some good growth, just need to transfer it to clean plates.


Extras: Filter Print Post Top
Offlinedextr0
Stranger
 User Gallery

Registered: 07/24/16
Posts: 431
Last seen: 20 days, 6 hours
Re: My intro... [Re: Pastywhyte]
    #23989444 - 01/07/17 12:33 PM (7 years, 23 days ago)

Fuck.
Ok well, should I let the first plate grow out a bit before transfering? I switched to pdya because it was whispy growth on PDA.

Just to be sure...satellites are the lump mycellia??


Extras: Filter Print Post Top
InvisiblePastywhyteMDiscord
Say hello to my little friend
Male User Gallery


Registered: 09/15/12
Posts: 37,810
Loc: Canada Flag
Trusted Cultivator
Re: My intro... [Re: dextr0]
    #23989515 - 01/07/17 01:01 PM (7 years, 23 days ago)

And any specks that show up on the perimeter. You want a clean plate with none of those things.


Extras: Filter Print Post Top
Offlinedextr0
Stranger
 User Gallery

Registered: 07/24/16
Posts: 431
Last seen: 20 days, 6 hours
Re: My intro... [Re: Pastywhyte]
    #23994830 - 01/09/17 10:45 AM (7 years, 21 days ago)

I'm still confused on what I should do with this plate. What I would have taken yesterday (10 o'clock; yesterdays growth not today's below) is now puffed up. Do I need to let it grow out more till I get flat growth??
If not can someone hip me to where they would take from on this plate. Thanx.


Extras: Filter Print Post Top
InvisiblePastywhyteMDiscord
Say hello to my little friend
Male User Gallery


Registered: 09/15/12
Posts: 37,810
Loc: Canada Flag
Trusted Cultivator
Re: My intro... [Re: dextr0]
    #23994905 - 01/09/17 11:16 AM (7 years, 21 days ago)

Transfer from 10 o'clock to a new plate.


Extras: Filter Print Post Top
Offlinedextr0
Stranger
 User Gallery

Registered: 07/24/16
Posts: 431
Last seen: 20 days, 6 hours
Re: My intro... [Re: Pastywhyte]
    #24012437 - 01/15/17 03:27 PM (7 years, 15 days ago)

How do these look and what should I do with last plate? Can I use any of it??There is beginning to be ropey mycelium one one side. I will take transfer but is any of other plate good? Sorry for trash pictures.



Edited by dextr0 (01/15/17 03:28 PM)


Extras: Filter Print Post Top
Offlinedextr0
Stranger
 User Gallery

Registered: 07/24/16
Posts: 431
Last seen: 20 days, 6 hours
Re: My intro... [Re: dextr0]
    #24015362 - 01/16/17 05:30 PM (7 years, 14 days ago)

Anyone wanna chime in?? I hate doing SAB work, but would rather do it sooner than later if need be. If things are looking good it would be nice to chill knowing all is well so far.


Extras: Filter Print Post Top
InvisibleMycolorado
Hobbyist
Male User Gallery

Registered: 07/23/16
Posts: 8,529
Loc: Interdimensional Bootcamp
Trusted Cultivator
Re: My intro... [Re: dextr0]
    #24015573 - 01/16/17 07:02 PM (7 years, 14 days ago)

Dude, things are looking good...settle down.  You can use whatever you want as long as it's not mold or bacteria.


Extras: Filter Print Post Top
InvisiblemushboyMDiscord
modboy
 User Gallery


Registered: 04/24/05
Posts: 32,281
Loc: where?
Trusted Cultivator
OG Cultivator
Re: My intro... [Re: dextr0]
    #24015578 - 01/16/17 07:03 PM (7 years, 14 days ago)

3,4,5 have bacteria or something. those bumps/abnormal growth bumps.. whatever but i would transfer again. all three have good leading edge growth. easy.

last pic cant tell.

1 and 2 look good.

SAB gets easier. its cumbersome at first. do mock stuff with it. i found its way easier for me to stand up while i work. so i have mine up on a table.


Extras: Filter Print Post Top
Offlinedextr0
Stranger
 User Gallery

Registered: 07/24/16
Posts: 431
Last seen: 20 days, 6 hours
Re: My intro... [Re: mushboy]
    #24015648 - 01/16/17 07:33 PM (7 years, 14 days ago)

Thanx fellas. I am always in a hurry when doing SAB work. I seriously hate it because its so delicate and I haven't got it down.
On top of that I don't really live anywhere. So I've made some promises that I need to b keeping if I want to have a spot to be doing this hobby of mine. That means growing. So I'm trying to get on it.

I don't kno why I'm getting satellites. The growth is very tomentose from all. 2 is second transfer from spore.
4&6 are the unknown culture. All mycelia grown has been very tomentose period. 4 is a transfer from 6.
The rest are PE6. Mostly from pins that I had pop up from agar.

I stopped having milky bacteria pop up. Stopped having trich or green mold come up. I can leave plates for weeks and they show no contamination. In fact I like to leave them because it gets rid of condensation...that's another story though. I don't kno what's going wrong. There are no visible signes of contamination except the bumps and tomentose growth.


Extras: Filter Print Post Top
OfflineThedillestpickle
cultured
Male


Registered: 02/02/16
Posts: 1,170
Loc: Canada Flag
Last seen: 3 years, 7 months
Re: My intro... [Re: dextr0]
    #24015677 - 01/16/17 07:40 PM (7 years, 14 days ago)

what are your plans with the agar?  Are you dropping wedges or making a LC or planning to blend it in sterile water?(sorry if I missed it didn't read the first couple pages)


Extras: Filter Print Post Top
Offlinedextr0
Stranger
 User Gallery

Registered: 07/24/16
Posts: 431
Last seen: 20 days, 6 hours
Re: My intro... [Re: Thedillestpickle]
    #24015708 - 01/16/17 07:47 PM (7 years, 14 days ago)

Dropping wedges to grain. I also want to make a broth to fill syringes with to have on stand by or trade with my homeboy.
If I make a clean lc or li I'll knock up so many different things it won't be funny.
I really need to get agar sterile procedure down though as I've got countless dried pins I would like to test out, and some dried reishi fruit I'd like to clean up also.


Extras: Filter Print Post Top
Offlinedextr0
Stranger
 User Gallery

Registered: 07/24/16
Posts: 431
Last seen: 20 days, 6 hours
Re: My intro... [Re: dextr0]
    #24034097 - 01/23/17 06:39 PM (7 years, 7 days ago)

Put in some work.
I have 4 brf cakes in quart zip locks. These will either be spawned to a small tray or made into slurry or just fruited in jar.
I have one brf that's left over from the cakes little less then a half pint. This will be slurry to bulk. Probably straw.
I also have three pom tek jars. For some reason the first one I made completely stalled half way. It turned super watery...so I'm thinking bacteria. Then other that I made with cornmeal took off good then slowed down the last couple days. Again water is a problem. I poured off access water today and shook up both jars. The cornmeal one was still growing this morning but I have little hope for the one made with brf.
I also have four wbs mixed with verm. Just experimenting cause that's what I do.
I'll keep posting to c results. Good or bad.


Extras: Filter Print Post Top
Offlinedextr0
Stranger
 User Gallery

Registered: 07/24/16
Posts: 431
Last seen: 20 days, 6 hours
Re: My intro... [Re: dextr0]
    #24035720 - 01/24/17 10:51 AM (7 years, 6 days ago)

So I go to my homies house to check how his jars are going. I hooked him up with some Teoanacatl spores off a print and we drunkenly proceed to make a syringe. I did that part cause he has a habit of fuckin Shit up and I don't need my print fucked  off...I have him knock up some jars. Somehow (ie. drunk mfs) he only ends up knocking up two jars with the whole syringe. IM just like omg this dumb MF. But he's a homie/homo (jk, kind of) I let him make it.
Long story short one did good one contaminated. I took the contaminated one home and cleaned it
up.
Pics.
The good cake fruiting. He was scared to roll or dunk  but next time he will. Pussy boy.
I also told him to put a plastic kid under his cake. Any advice I'll pass on and would be appreciated.

First agar plate from dirty culture. As soon as I saw clean growth I transferred. I wanted to wait for better growth but I had a thin growth coming from one of the spots where I put the brf piece to agar. IM just saying because from the top you couldn't see it it was so thin. Only way was to shine light on side if plate. It was that thin. Showed me the importance of watching  my plates every day if possible (mycelia can grow over contamination if u don't see it grow over it's just sitting there hiding active and waiting for u to let it do it's thing once u use the contaminated piece) and looking at your plates from all angles.

Last pic is the transfer I took. Looks clean to me. Weird because it's rhizomorphic growth. And this is actually only the the second transfer from spore. And I didn't let it grow out much at all.




Extras: Filter Print Post Top
Offlinedextr0
Stranger
 User Gallery

Registered: 07/24/16
Posts: 431
Last seen: 20 days, 6 hours
Re: My intro... [Re: dextr0]
    #24056055 - 01/31/17 11:07 PM (6 years, 11 months ago)



Extras: Filter Print Post Top
Offlinedextr0
Stranger
 User Gallery

Registered: 07/24/16
Posts: 431
Last seen: 20 days, 6 hours
Re: My intro... [Re: dextr0]
    #24093195 - 02/15/17 03:45 PM (6 years, 11 months ago)

1. Inoculation loop whatever. Bread ties kept giving me bacteria. Not this.
2-3. Teonanacatl cubensis on PF cake. Day 24
4. Unknown cubensis. Finally going to print. I have a BRf cake fully colonized, just waiting for pins to loosen jar lids. I never got pins on agar from this one so I got a dish I was going to toss threw in some wet vermiculite and fan with lid of chamber couple times a day. Finally pinned.
5-8. Unknown cubensis mycellia on pbph (potato bee pollen honey) agar. Always grows crazy. I wonder if its getting too old. It doesnt like to make rope mycelia.




Extras: Filter Print Post Top
Offlinedextr0
Stranger
 User Gallery

Registered: 07/24/16
Posts: 431
Last seen: 20 days, 6 hours
Re: My intro... [Re: dextr0]
    #24098932 - 02/17/17 09:43 PM (6 years, 11 months ago)

Teonanacatl cubensis.


The unknown cubensis I've been chasing for 6 1/2 months...if only I had cased an agar plate sooner...


Edited by dextr0 (02/17/17 09:46 PM)


Extras: Filter Print Post Top
Offlinedextr0
Stranger
 User Gallery

Registered: 07/24/16
Posts: 431
Last seen: 20 days, 6 hours
Re: My intro... [Re: dextr0]
    #24101500 - 02/18/17 09:55 PM (6 years, 11 months ago)

PE6 on agar:




Edited by dextr0 (02/19/17 06:24 AM)


Extras: Filter Print Post Top
Offlinedextr0
Stranger
 User Gallery

Registered: 07/24/16
Posts: 431
Last seen: 20 days, 6 hours
Re: My intro... [Re: dextr0]
    #24119414 - 02/25/17 06:07 PM (6 years, 10 months ago)

Teonanacatl 2nd flush



Extras: Filter Print Post Top
Offlinedextr0
Stranger
 User Gallery

Registered: 07/24/16
Posts: 431
Last seen: 20 days, 6 hours
Re: My intro... [Re: dextr0]
    #24124712 - 02/27/17 08:37 PM (6 years, 10 months ago)



Edited by dextr0 (02/27/17 08:39 PM)


Extras: Filter Print Post Top
Offlinedextr0
Stranger
 User Gallery

Registered: 07/24/16
Posts: 431
Last seen: 20 days, 6 hours
Re: My intro... [Re: dextr0]
    #24519273 - 07/30/17 09:37 PM (6 years, 5 months ago)

I'm still having trouble with grains...
This is Ape. I shook early (like at 15%), friend came over said I should shake again. Thing that bothers me is a lot of ppl say never shake a baby Ape. Also the moisture bothers me and has me not wanting to spread bacteria if its there.
Any help appreciated.



Edited by dextr0 (07/30/17 09:39 PM)


Extras: Filter Print Post Top
Offlinedextr0
Stranger
 User Gallery

Registered: 07/24/16
Posts: 431
Last seen: 20 days, 6 hours
Re: My intro... [Re: dextr0]
    #24567801 - 08/20/17 04:09 PM (6 years, 5 months ago)

1,2,3 Ape
4 Pan Tropicalis
5,6 The Unknown culture Ive been chasing. 5 more quarts of Ape and Ill be turning back to this culture. Infact im making Five quarts of oats now to knock up with this culture while the next Ape finish. Notice the intermediate linear type mycelium Try as I might i cant get full rhizomorphic myc.




Edited by dextr0 (08/20/17 04:12 PM)


Extras: Filter Print Post Top
Offlinedextr0
Stranger
 User Gallery

Registered: 07/24/16
Posts: 431
Last seen: 20 days, 6 hours
Re: My intro... [Re: dextr0]
    #24576415 - 08/24/17 11:50 AM (6 years, 5 months ago)

All my shoeboxes went bad. Some kind of grey mold. But my spawn was trash. Had to be bacterial at first then mold took over.

I did get an OZ from two shoeboxes. One of the boxes only produced like ten fruit. No picture but it did produce this 24g fruit (Cartman)

New jars started. These grains were dried well. I feel a bit more confident in them. We will see soon. This next grow will be my last APE grow for awhile.They make me feel too racey, something I really do not enjoy. They are beautiful tho.





Extras: Filter Print Post Top
InvisibleMycolorado
Hobbyist
Male User Gallery

Registered: 07/23/16
Posts: 8,529
Loc: Interdimensional Bootcamp
Trusted Cultivator
Re: My intro... [Re: dextr0]
    #24576448 - 08/24/17 11:59 AM (6 years, 5 months ago)

:thumbup:


Extras: Filter Print Post Top
InvisibleThirdEyeHigh
Lone Farmer
I'm a teapot

Registered: 07/15/17
Posts: 95
Loc: Guantanamo Bay
Re: My intro... [Re: dextr0]
    #24576451 - 08/24/17 12:00 PM (6 years, 5 months ago)

You should make a new thread for your grow logs bro, you arent a noob anymore!  :jah:


Extras: Filter Print Post Top
Offlinedextr0
Stranger
 User Gallery

Registered: 07/24/16
Posts: 431
Last seen: 20 days, 6 hours
Re: My intro... [Re: ThirdEyeHigh]
    #24707488 - 10/13/17 03:36 PM (6 years, 3 months ago)

Im still very much a noob...lazyness being the biggest thing holding me back.

Ive got This Unknown variety in the works again, total of a year and almost three months of work. This is shoebox 6qt at day 6. 1qt coir to 1Qt oat spawn. Half a qt on top as casing.


Edited by dextr0 (10/18/17 07:35 AM)


Extras: Filter Print Post Top
Offlinedextr0
Stranger
 User Gallery

Registered: 07/24/16
Posts: 431
Last seen: 20 days, 6 hours
Re: My intro... [Re: dextr0]
    #24718823 - 10/18/17 07:38 AM (6 years, 3 months ago)

Day 11. Pins came up two days ago...they are really small. I leave the lid off so little bit of bruising from drying...I mist probably once a day...
Any advice appreciated.



Extras: Filter Print Post Top
OfflinecronicrFacebook
 User Gallery


Registered: 08/07/11
Posts: 61,436
Loc: Van Isle Flag
Last seen: 2 years, 9 days
Trusted Cultivator
Re: My intro... [Re: dextr0]
    #24718929 - 10/18/17 08:56 AM (6 years, 3 months ago)

Bump up the misting it looks great


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


Extras: Filter Print Post Top
Offlinedextr0
Stranger
 User Gallery

Registered: 07/24/16
Posts: 431
Last seen: 20 days, 6 hours
Re: My intro... [Re: cronicr]
    #24718948 - 10/18/17 09:12 AM (6 years, 3 months ago)

Quote:

cronicr said:
Bump up the misting it looks great




Will do. Thanx for looking and helping bro.


Extras: Filter Print Post Top
Offlinedextr0
Stranger
 User Gallery

Registered: 07/24/16
Posts: 431
Last seen: 20 days, 6 hours
Re: My intro... [Re: dextr0]
    #24723836 - 10/20/17 06:44 AM (6 years, 3 months ago)

Day 13


Extras: Filter Print Post Top
Offlinedextr0
Stranger
 User Gallery

Registered: 07/24/16
Posts: 431
Last seen: 20 days, 6 hours
Re: My intro... [Re: dextr0]
    #24728822 - 10/22/17 07:13 AM (6 years, 3 months ago)

Day 15


Extras: Filter Print Post Top
Offlinedextr0
Stranger
 User Gallery

Registered: 07/24/16
Posts: 431
Last seen: 20 days, 6 hours
Re: My intro... [Re: dextr0]
    #24733958 - 10/24/17 12:34 PM (6 years, 3 months ago)

Day 17
I dont know what im doing wrong. Caps are opening while they are still very small. Would overwatering cause this??
Any help appreciated. Thanx.



Extras: Filter Print Post Top
InvisibleGuy1980
Registered: 09/11/12
Posts: 723
Re: My intro... [Re: dextr0]
    #24734109 - 10/24/17 01:34 PM (6 years, 3 months ago)

I had a coir/grain mix which was soaked (way too much water), the shrooms were tiny.  And I mean a massive pinset and fruits, none bigger than an inch.


Extras: Filter Print Post Top
InvisiblePastywhyteMDiscord
Say hello to my little friend
Male User Gallery


Registered: 09/15/12
Posts: 37,810
Loc: Canada Flag
Trusted Cultivator
Re: My intro... [Re: Guy1980]
    #24734121 - 10/24/17 01:43 PM (6 years, 3 months ago)

Looks dry as hell.


Extras: Filter Print Post Top
Offlinedextr0
Stranger
 User Gallery

Registered: 07/24/16
Posts: 431
Last seen: 20 days, 6 hours
Re: My intro... [Re: Pastywhyte]
    #24734143 - 10/24/17 01:54 PM (6 years, 3 months ago)

Quote:

Pastywhyte said:
Looks dry as hell.




Which is weird because I dunked it like 4 hrs before because I thought the same thing. Sub is heavy af.


Extras: Filter Print Post Top
InvisiblePastywhyteMDiscord
Say hello to my little friend
Male User Gallery


Registered: 09/15/12
Posts: 37,810
Loc: Canada Flag
Trusted Cultivator
Re: My intro... [Re: dextr0]
    #24734169 - 10/24/17 02:06 PM (6 years, 3 months ago)

The sub might be waterlogged but the surface is dried out and dead. Which is probably why the fruits are opening.


Extras: Filter Print Post Top
Offlinedextr0
Stranger
 User Gallery

Registered: 07/24/16
Posts: 431
Last seen: 20 days, 6 hours
Re: My intro... [Re: Pastywhyte]
    #24734314 - 10/24/17 03:18 PM (6 years, 3 months ago)

Got it. Thank yall for the replys.
I guess Ill cover it and let it do its thing.
This is another box 7 days in. Same unknown variety.


Extras: Filter Print Post Top
Offlinedextr0
Stranger
 User Gallery

Registered: 07/24/16
Posts: 431
Last seen: 20 days, 6 hours
Re: My intro... [Re: dextr0]
    #24738644 - 10/26/17 09:15 AM (6 years, 3 months ago)

Fuckkkk!!
Took a pic this morning and as I was putting it back dropped the damn box on the ground.
So I harvested. I picked every little mushie I could see.
Sad thing is I was going to give away some prints. Had all the stuff ready. Lol I know most would be like wtf you think I want that variety for. Its the most MDMA like shroom Ive ever eaten. The body high is super impressive.

Anyway here it is 157.9 wet.
Heres the next box too. Nice glistin to the surface, knotting up too.




Extras: Filter Print Post Top
Offlinedextr0
Stranger
 User Gallery

Registered: 07/24/16
Posts: 431
Last seen: 20 days, 6 hours
Re: My intro... [Re: dextr0]
    #24747374 - 10/30/17 10:30 AM (6 years, 2 months ago)

Day 12


Extras: Filter Print Post Top
Offlinedextr0
Stranger
 User Gallery

Registered: 07/24/16
Posts: 431
Last seen: 20 days, 6 hours
Re: My intro... [Re: dextr0]
    #24826585 - 12/04/17 07:43 PM (6 years, 1 month ago)

Fruits from the Unknown. This will conclude my thread. I accomplished what I wanted, to save this variety. Im exeptionally happy with the results. This is my favorite variety so far. Very MDMA like. Only complaint is it lasts only 4 or 5 hrs and tolerance builds fast. This replaced my APE as I like the feel of these more. Potency wise it hangs with APE.



Extras: Filter Print Post Top
Offlinedextr0
Stranger
 User Gallery

Registered: 07/24/16
Posts: 431
Last seen: 20 days, 6 hours
Re: My intro... [Re: dextr0]
    #24897465 - 01/07/18 09:01 PM (6 years, 23 days ago)

Later flush. Finally got them looking right. Only thing I can attribute it to is better surface conditions. Followed the instructions of the thread to the T...got better looking mushies.
https://www.shroomery.org/forums/showflat.php/Number/23999053
Glad to see the Unknown in all its glory finally.



Extras: Filter Print Post Top
Jump to top Pages: 1 | 2 | 3 | 4 | 5 | 6 | 7  [ show all ]

Shop: PhytoExtractum Kratom Powder for Sale   Left Coast Kratom Buy Kratom Extract   Bridgetown Botanicals Bridgetown Botanicals   North Spore Bulk Substrate   Original Sensible Seeds Autoflowering Cannabis Seeds   Mushroom-Hut Liquid Cultures   Kraken Kratom Kratom Capsules for Sale


Similar ThreadsPosterViewsRepliesLast post
* Spawning Oysters to Cardboard omegafaust 1,646 12 02/17/13 09:03 AM
by dizzy_simmons
* Mycelia on cardboard!?!?!? LaCasta 1,942 3 07/16/02 04:12 AM
by kykeon
* Cardboard TEK Softpaw 2,067 9 07/29/12 05:25 PM
by Doc_T
* Cardboard Growing??! TheShroomingAtheis 831 5 07/05/12 03:10 AM
by TheShroomingAtheis
* Cardboard Tech Question Sailorzen 605 4 09/22/12 12:23 PM
by Grumpyorc
* Can Mycelia Be Stored (Colonized Grain) Hobosapien 1,013 2 03/03/07 10:41 PM
by odium33
* can anyone link me to the azure cardboard 'tek'?/ thread/post Biffzilla 426 1 04/14/11 10:46 AM
by Biffzilla
* Myce piss = stress & bacteria? buffer 2,121 18 09/03/11 02:24 AM
by buffer

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
5,354 topic views. 16 members, 168 guests and 33 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.066 seconds spending 0.011 seconds on 14 queries.