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Mycolorado
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Registered: 07/23/16
Posts: 8,529
Loc: Interdimensional Bootcamp
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Re: My intro... [Re: dextr0]
#23830281 - 11/13/16 07:44 PM (7 years, 2 months ago) |
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Ha, that's what I assumed it was but wasn't sure. Crazy!
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dextr0
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Registered: 07/24/16
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Last seen: 20 days, 4 hours
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I like the idea of short time for pinning so I can transfer to agar.15 days and no pins. But I'm using regular grocery store tek agar. I mean that's where I thought I would start. I need to read the tek again. Thing is I like to read a whole thread. Its a long thread. And I get side tracked along the way checking out threads mentioned along the way. Yes I'm that guy. I forgot exactly why I was gonna mention this but the pins take average two three days to show growth. Lol. Its 420 round here.
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dextr0
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Re: My intro... [Re: dextr0]
#23837275 - 11/15/16 11:05 PM (7 years, 2 months ago) |
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dextr0
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Re: My intro... [Re: dextr0]
#23838827 - 11/16/16 01:28 PM (7 years, 2 months ago) |
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Update: don't know if this is brusing or something else. Better pics in a bit. I constantly squeeze the sides for GE. So maybe maybe not. Any opinions??
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dextr0
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Re: My intro... [Re: dextr0]
#23842004 - 11/17/16 02:34 PM (7 years, 2 months ago) |
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Pastywhyte
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Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: My intro... [Re: dextr0]
#23842016 - 11/17/16 02:38 PM (7 years, 2 months ago) |
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That ain't looking happy at all
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dextr0
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Last seen: 20 days, 4 hours
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Yes I know. Its ok. I'm gonna start some more spawn soon. Do things right. Finally got a clean plate of the PE6. While I'm doing that grow I'm going to clean this culture up and give it another go when I do. I have a few grains left over on the bottom of a jar. I have another pic of the blue to add. Gotta charge phone. I also let the lid off in a makeshift SAB to give it some good GE and get some of that moisture out of the box. I secretly hope it initiates pinning.
I'm also checking out the potato dextrose broth. I recently saw a post saying it can be used to basicly grow a stronger mycelia when working with a old culture. This specific culture did have a very wispy light mycelia.
Quote:
Grundalizer said: Any white mycelia, fluffy or not, which is derived from a known spore addition to a sterile media under sterile or semi-sterile conditions and is growing out of the area you doped the media with, is 99.99% what you are looking for. Cobwebby growth does not equal contamination. I'm a microbiologist and mycologist by trade and I'm amazed at how often I see people on hear freaking out about contamination and throwing things out (not trying to call you out, I just don't post much, and am trying to start to more). If you have anything growing on a plate, just pick a corner with a sterile scalpel or sterile toothpick and re-plate...subculture till purity.
Any moulds are easy to see because of their almost instantaneous rapid color changes due to conidia with spores forming which change color often due to their age.
Any true basidiomycete will stay in a white mycelial growth, and yes, often if I regenerate old spores or super old dried our desicated agar plates, initial growth is very very very wispy.
I found sub-culturing doesn't help, but taking some and dropping it into sterile broth, like potatoe dextrose broth, yields very healthy and dense mycelial growth...which I then re-plate onto solid agar.
https://www.shroomery.org/forums/showflat.php/Number/23824055/fpart/1/vc/1/nt/2
Agar broth, throw out your crap LCs https://www.shroomery.org/forums/showflat.php/Number/13781184/fpart/1/vc/1
Edited by dextr0 (11/17/16 03:23 PM)
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Pastywhyte
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Registered: 09/15/12
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Loc: Canada
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Re: My intro... [Re: dextr0]
#23842279 - 11/17/16 03:42 PM (7 years, 2 months ago) |
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I use LC all the time. Wedges to LC works great but lately I do this.
https://www.shroomery.org/forums/showflat.php/Number/23740857
Biggest issue with LC is the use of dirty inoculate (spores) and the second biggest issue is the use of sugary media which creates sluggish growth.
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dextr0
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Finally. Mission accomplished. Pins from the contaminated tub. This is all I wanted. I will now transfer to agar and clean up. I was happy because I have plates that are old as hell. In fact this is the dry mushroom culture that I've been trying to regenerate for three months now. So I have a young culture to work with and I know it will fruit.. Which I was beginning to have doubts about. I was actually contemplating throwing it out today.
Edited by dextr0 (11/22/16 08:12 AM)
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Mycolorado
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Registered: 07/23/16
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Re: My intro... [Re: dextr0]
#23856413 - 11/22/16 07:30 AM (7 years, 2 months ago) |
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Right on! Your plan sounds good. You should keep this thread going to illustrate how to go from a questionable grow to a nice clean pruductive one.
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dextr0
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This print came in from FSRE: Teonanacatl This strain was observed and collected for the 1st time by Mauritius (founder of the pro-fungi, Brasil) in the Lowland region Careiro, state of Amazonas, Brazil. During the rainy season extending from September to May, buffalo breeders bring their pets to farms flooded by the road BR 319 that connects the Careiro Varzea Careiro to Brown. With the end of the rains the animals are transported to other areas. In July of 2008 Mauritius entered a farm and collected a mushroom of the genus Psilocibec cubensis from the buffalo manure. The spores of this strain where clean on agar and sectors of mycelium where isolated for 1 year to select the best growing mycelium on rice and corn. The name is in reference to the site Teonanacatl.
On a side note my Redboy never showed up. Guess I got jacked. *shrug*
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dextr0
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Re: My intro... [Re: dextr0]
#23871788 - 11/27/16 11:41 AM (7 years, 2 months ago) |
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Here's a cool picture of some PE6 colonizing some Desmanthus Illinoensis roots. I will make a substrate containing small pieces mixed in as in experiment in the future. I realize this has contams I'm just trying different things. Putting a small stick in with agar would make my transfers easier and I believe cleaner. This container sucked because it was not clear so I opened constantly. Like I said was experimenting only.
 Anyway I've got some WBS ready for inoculation. Probably agar wedges to the grain. I'm kind of curious of how some sterile water into the dish with pom drawn up into syringe would work out. Thing is I'm not sure its clean as its hard to tell because of scrubby.
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dextr0
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Re: My intro... [Re: dextr0]
#23989387 - 01/07/17 12:08 PM (7 years, 23 days ago) |
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OK so been busy being a whore...I've been doing lil things here and there though so new shit. I for the life of me couldn't get a sterile plate... I've got two now but its pure luck I feel.

 I did stop using bread tie inoculation loops. I want to try more but I happened to change to a syringe and have four clean transfers so far. I made a couple vtek jars...one contaminated bad.


 I also made another with corn meal instead and the POM's cut into 8 pieces so I can inoculate then use as spawn. I just haven't inoculated yet.
 Talked a friend into growing. Who knos what he will do long term he's very lazy...he did go buy huge bags of verm, perlite, made a SAB, jars, and grinded out bunch of bfr. So I hooked him up with some spores (we made a syringe off the Teo from FSRE, drunk as fuck having no idea how to do it exactly but I got it done) and knocked up a few jars. One contaminated so I brought it home grabbed a few pieces of mycelia and am cleaning it on agar now.

A PE6 off agar.


All PE6 jars pinned like crazy.

Still can't get my WBS hydrated right. Tried a lot of different things but no go still so I'm working on that still.
Links I've been checking out interested in...maybe interest others:
Looking for sources of dextrose I ran into this... The Experiments, vol. 1: Bee Pollen Granules and SpawnMate https://www.shroomery.org/forums/showflat.php/Number/2301527
Brothing... *NEW* Alien Broth Tek --- with pictures! https://www.shroomery.org/forums/showflat.php/Number/1656605#1656605
Make sure to read the caption at bottom of pics here on this one.
"For PDY Broth: made the same way but omitting agar. For Acide PDYA: add 1 ml Lactic Acid to liquid media just before pouring plates." http://plantpath.psu.edu/facilities/mushroom/cultures-spawn/media-preparation
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: My intro... [Re: dextr0]
#23989399 - 01/07/17 12:11 PM (7 years, 23 days ago) |
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Both of those plates have satellites but they could be easily cleaned up. There is some good growth, just need to transfer it to clean plates.
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dextr0
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Registered: 07/24/16
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Last seen: 20 days, 4 hours
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Fuck. Ok well, should I let the first plate grow out a bit before transfering? I switched to pdya because it was whispy growth on PDA.
Just to be sure...satellites are the lump mycellia??
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Pastywhyte
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Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: My intro... [Re: dextr0]
#23989515 - 01/07/17 01:01 PM (7 years, 23 days ago) |
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And any specks that show up on the perimeter. You want a clean plate with none of those things.
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dextr0
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Registered: 07/24/16
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Last seen: 20 days, 4 hours
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I'm still confused on what I should do with this plate. What I would have taken yesterday (10 o'clock; yesterdays growth not today's below) is now puffed up. Do I need to let it grow out more till I get flat growth?? If not can someone hip me to where they would take from on this plate. Thanx.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: My intro... [Re: dextr0]
#23994905 - 01/09/17 11:16 AM (7 years, 21 days ago) |
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Transfer from 10 o'clock to a new plate.
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dextr0
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How do these look and what should I do with last plate? Can I use any of it??There is beginning to be ropey mycelium one one side. I will take transfer but is any of other plate good? Sorry for trash pictures.
Edited by dextr0 (01/15/17 03:28 PM)
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dextr0
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Re: My intro... [Re: dextr0]
#24015362 - 01/16/17 05:30 PM (7 years, 14 days ago) |
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Anyone wanna chime in?? I hate doing SAB work, but would rather do it sooner than later if need be. If things are looking good it would be nice to chill knowing all is well so far.
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