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dextr0
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Registered: 07/24/16
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Re: My intro... [Re: dextr0]
#23781326 - 10/28/16 09:26 PM (7 years, 3 months ago) |
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 1. PE6 from pin. Pom agar. 2. Unknown variety (cubensis). Just cool pic sideways tho. 3-5. Contamination?? Anyone have any idea what kind?? I tried get good shots.
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dextr0
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Re: My intro... [Re: dextr0]
#23785560 - 10/30/16 01:52 PM (7 years, 2 months ago) |
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Scrubbers or POM's that are good. Didn't even rinse em off just poured agar on top and pced. Growth started the next day. Clean, has been so far anyway. Brand is scrub buddies from dollar tree (USA). I'm posting because I know some have had trouble getting workable POM's. (Pasty, if u still ain't got a source u do now) Figured I would help by giving good brand and location to find. Also got some syringes. 20 gage, but I'm sure I can make em work.
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dextr0
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Re: My intro... [Re: dextr0]
#23794839 - 11/02/16 03:06 PM (7 years, 2 months ago) |
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I keep getting the milky bacteria on my agar no matter how sterile I am. I took care of the green mold by cleaning up the area and throwing anything contaminated away. I need some pointers if someone wouldn't mind sharing.
Agar recipe: 3/4 tbs agar 1 tbs potato flakes 1 drop of honey 125 mil water
I also have a cheap 6 qt PC. Its a rocker,tDenmark brand. I PC from 45 min to 60 min. Slow rock because if u turn down slightly u get no rock at all.
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Mycolorado
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Re: My intro... [Re: dextr0]
#23795098 - 11/02/16 04:28 PM (7 years, 2 months ago) |
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Hey dextr0, you should weigh the dry ingredients as opposed to measuring them and might consider some petri dishes...they're cheap and make plate work much easier. PC your agar for 30 minutes at 15psi and pour in a SAB. You should be good to go.
Standard 2% nutrient medium recipe (you can make whatever volume you want):
1000ml H2O 20g agar 20g nutrient (malt extract, potato flake, dog food)
Edited by Mycolorado (11/02/16 04:30 PM)
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spacechildo
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his recipe sounds good, bacteria comes from poor sterile tech or really fucked syringes.
its all about practice, smooth movements, keep air still, dont hover hands above open media. and if you see clean growth take a transfer quick and dont let bacteria run over your plates.
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dextr0
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Yea. Been meaning to do that since I started agar. Store was out of preferred scale so I basically tried to just feel it out and keep it comfortable. I try to keep it as close to pasty math as much as possible tho. U really think that's my problem? Either way yea I need a scale so I'm get one. I really wonder if my PC even reaches 15psi. Thanks for helping.
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Mycolorado
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Fosho...just thought I'd put up the standard mix as it's easy to scale.
dextr0, You might pour a plate and set it to the side (don't transfer anything to it)to see if anything grows on it...will help narrow down the problem. If something grows, you know it's contaminating when you pour and not when you transfer or that there is an issue with the prep...
Edit If your PC doesn't hit 15psi just let it run for 45-hour.
Edited by Mycolorado (11/02/16 04:52 PM)
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dextr0
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Thanks fellas. I guess I just need to work on smooth movements. I'm not using syringes at all at this point. Have some but haven't used. I'm stuck on getting a clean plate, which I can't seem to get. Im honestly just trien to actually pay attention to the agar growing over contams( i mean not trying to get those pieces). Ive been under the assumption that if I had clean looking plate the mushies won. Little did I know they ride along. So I'm dealing with a bunch of dirty cultures. And constant change of environment from moving a lot. I clean up as best I can but u know... I'm going to try to transfer a piece of agar to another agar. See if its my sterile tek. I have some premade agar that seems pretty clean. No milky look. Anyway I just add to plates or pods or jars and PC. Ive been planing just not sure bout some things.
Edited by dextr0 (11/02/16 05:56 PM)
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dextr0
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Re: My intro... [Re: dextr0]
#23806328 - 11/06/16 12:04 PM (7 years, 2 months ago) |
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This is the half gallon that never fully colonized spawned to unpasteurised straw. I just wanted it to work so bad cause its the last of cube var. The other plates got contams. I don't even know if its a fruiting culture. I've had some jars of agar for months now and zero pinning. It did come from a fruit tho.
Edited by dextr0 (11/06/16 04:24 PM)
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dextr0
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Re: My intro... [Re: dextr0]
#23807493 - 11/06/16 05:31 PM (7 years, 2 months ago) |
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How does this look?? 11 days.
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dextr0
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Re: My intro... [Re: dextr0]
#23821262 - 11/10/16 09:42 PM (7 years, 2 months ago) |
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I've got a question. If I use a bread tie as a loop how long do I need keep lighter on on metal to insure sterilization?? Or the flame I mean. This is the last thing I'm going to check before I just go for a new PC. The one I have is small af anyway and leaks water n shit. Was brand new but cheaply.
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dextr0
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Re: My intro... [Re: dextr0]
#23825050 - 11/12/16 06:59 AM (7 years, 2 months ago) |
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This is five days growth. Very slow if u ask me. So I'm wondering should I case now?? Should I wait it out? Should I take off all exposed straw give a couple days to recover, then case? Help me fuckers.
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Mycolorado
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Re: My intro... [Re: dextr0]
#23825080 - 11/12/16 07:22 AM (7 years, 2 months ago) |
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Best thing to do at this point is close it up and come back in 5 days. Also looks like it could have been pasteurized a bit better...there are some seeds germinating in there...what was that process?
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dextr0
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Hey Mycolorado thanks for stopping in. You see that's the whole problem. The spawn I used was sterilized, but I'm sure was bacterial as it never fully colonized (waited almost a month). I did use it no matter what. Just had a hunch it might work. The straw I used though was never pasteurized or sterilized. I kno I shouldn't have even tried but I had to.
I'm fine if nothing comes out of this, but I need some assistance to maybe pull it off. I just need one print to play with. The two pictures above were a comparison of what's going on. Same tub 5 days apart. The tub is going on three plus weeks.
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Pastywhyte
Say hello to my little friend



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Re: My intro... [Re: dextr0]
#23825338 - 11/12/16 09:21 AM (7 years, 2 months ago) |
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With uncolonized grain in there it's toast before it began. But if it was to have a fighting chance, straw needs to be cut fine, 1" to 2" max. Finer is better. Straight straw generates a lot of heat. It's better to have it mixed with some coir or manure or both.
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dextr0
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Thanx Pasty, u mean for next time right??
This is my biggest fear. That the casing will introduce more harmefull bacteria or mold and it ends all chances. Same if I was to add anything to it or add it to anything.
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Pastywhyte
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Re: My intro... [Re: dextr0]
#23827402 - 11/12/16 09:39 PM (7 years, 2 months ago) |
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Yeah I mean for next time. This time is pretty much fucked.
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dextr0
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Re: My intro... [Re: dextr0]
#23827449 - 11/12/16 09:59 PM (7 years, 2 months ago) |
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Lol. Thanx. Nice surprise just now. It looks clean. Pe6. I wonder if its truly clean though. Would b nice to have something for Christmas. I'm just looking for a clean culture. To do this shit right.
 I did try to make a transfer like a week ago...but I thought it had mycogone. It had a lot of condensation on sides and like 3 dots on mycelium... But those seem to have dried up along with the condensation. The transfer I think ended up bacterial so I threw it this one to the side for a little while. I should probably try another transfer.
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Mycolorado
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Re: My intro... [Re: dextr0]
#23828378 - 11/13/16 09:23 AM (7 years, 2 months ago) |
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What's that growing on?
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dextr0
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Hey Myc. Its on a pan scrubber. Idea is from here https://www.shroomery.org/forums/showflat.php/Number/20643482 I'm trying to find a way to make this work with a modified grocery store agar. Admittedly I need to go over the tek again. A few things I'm not sure of yet.
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