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Offlinedextr0
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My intro...
    #23511938 - 08/05/16 08:04 AM (7 years, 5 months ago)

OK I'll start off by saying I'm very ghetto right now...that means I have to be resourceful and make do. I'm making a log on all that I do and will be doing. As I progress more $ things will step up. But for now I make do. I have no prior experience I'm the cultivation of mushrooms, but  a experienced cannabis grower. I am or have been a member of countless cannabis forums and love to contribute and show people easy ways of doing things. My main interest was making plant fermented extracts for plants and soil management.
Just a little background...so I am here to learn and of course share experiences. I'm currently trying to use the cardboard method to try and get growth from a dried out mushroom. I mistakenly left one jar sealed up (baby food jar) and I noticed some wispy mycelia growing next to some healthy looking mycelia pieces ( of mushroom). Worried it might be cob web I transfered a piece of healthy looking mushroom to another jar with cardboard. This time I made a hole and stuffed poly in so it could breath. The other jar I set outside to see what happens. When I opened it last it had a rancid smell to it...?
With that said I decided cardboard is not for me right now experimentation can come later, right now I need for sure. So I broke down and went for agar agar. I picked up supplies yesterday and am going to be using agar, honey, dextrose, and brewers yeast. I also sent off to fSRE for a print. I have little to no money left so hopefully they send me my print need it.
Well that's my intro.
I've also got some Reishi that are dried and am going to see if they will work with either agar or cardboard.
I've got pics coming soon.
Any advice or input welcome..


Edited by dextr0 (08/05/16 08:17 AM)


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Offlinedextr0
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Re: My intro... [Re: dextr0]
    #23512035 - 08/05/16 08:46 AM (7 years, 5 months ago)

Can't seem to get pics on here...help anyone??


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Offlinea_stoned_sloth
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Re: My intro... [Re: dextr0]
    #23512058 - 08/05/16 08:55 AM (7 years, 5 months ago)

Under your name in the top left corner it says Pics. This is where you can upload and share on the forum.


--------------------
"As the garden grows, so does the gardener."


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Invisiblemupetmower
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Re: My intro... [Re: a_stoned_sloth]
    #23512061 - 08/05/16 08:57 AM (7 years, 5 months ago)



--------------------
-The wise man never stops seeking knowledge.


-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.


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OfflinePsykronik
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Re: My intro... [Re: mupetmower]
    #23512066 - 08/05/16 08:59 AM (7 years, 5 months ago)

Where you ever a member of overgr.w before it got shut down ?


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Offlinedextr0
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Re: My intro... [Re: Psykronik]
    #23512075 - 08/05/16 09:05 AM (7 years, 5 months ago)



1. dry mushroom with cardboard. Wispy growth thought it might be cob web mold.
2. Reishi mushrooms on log. Want to try cardboard method. Haven't got around to it yet.
3. The other piece of mushroom (cubensis) that had what I think is good mycelial growth. Transfered it to another jar so wispy growth from other mushroom piece would not contaminate if it is cob web.


Edited by dextr0 (08/05/16 09:17 AM)


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Offlinedextr0
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Re: My intro... [Re: dextr0]
    #23512086 - 08/05/16 09:09 AM (7 years, 5 months ago)

Thanx everyone. Probably doing this the hard way but oh well.
I was never on OG, I have been on cannabis world, icmag, and thcfarmer. I still visit from time to time but have moved to different hobbies.


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Invisiblemupetmower
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Re: My intro... [Re: dextr0]
    #23512090 - 08/05/16 09:10 AM (7 years, 5 months ago)

so that is what, again? the cardboard with pieces of mushroom on it? what is the mushroom in the top right pic? if  that what you put on the cardboard? im lost here, sorry man haha.


--------------------
-The wise man never stops seeking knowledge.


-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.


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InvisiblePastywhyteMDiscord
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Re: My intro... [Re: dextr0]
    #23512094 - 08/05/16 09:14 AM (7 years, 5 months ago)

That looks pretty gross. No telling what you have there. I suggest you get some spores from a reputable source and ditch the cardboard. Cardboard is great for low tek expansion of outdoor woodloving species. It's terrible for dunglovers that we are looking to bring indoors.


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Invisibleoontribe
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Re: My intro... [Re: mupetmower]
    #23512102 - 08/05/16 09:18 AM (7 years, 5 months ago)



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Offlinedextr0
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Re: My intro... [Re: Pastywhyte]
    #23512125 - 08/05/16 09:22 AM (7 years, 5 months ago)

I agree it looks gross. Wet cardboard does not have the appeal that agar has. Bottom line, I used a piece of dried mushroom...sandwiched between two cardboard pieces and waited for mycelial growth.
Until I get more funds I will have to make do. And thats what I'm doing.
I know this will work I just need time to work on it.


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InvisiblePastywhyteMDiscord
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Re: My intro... [Re: dextr0]
    #23512144 - 08/05/16 09:29 AM (7 years, 5 months ago)

My advice is that if you can't afford a few bucks to do things in this hobby right, your priorities are misplaced. $100 or less can get you going on the PF tek. What you have going on there will be very hard pressed to expand into fruits.

Good luck.


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Offlinedextr0
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Re: My intro... [Re: Pastywhyte]
    #23512184 - 08/05/16 09:45 AM (7 years, 5 months ago)

OK how bout this. I'm going to mix up the agar. Then transfer a new piece of dried mushroom. That will make things pretty. I'm still going for the cardboard method in the other jars.
By the by...the Tek I'm using was explained by Roger Rabbit himself in past posts. I say this to add a little credibility to my findings. I'm not completely new to this. I've at least been doing my research.
Again Thanx to all who responded.


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InvisiblePastywhyteMDiscord
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Re: My intro... [Re: dextr0]
    #23512204 - 08/05/16 09:53 AM (7 years, 5 months ago)

It will all be for nothing depending on how that mushroom was dried. You need to put stuff in context. If RR was talking about fruits specially grown in aseptic conditions and dried without heat (and that is likely what he was talking about) then yes, that can work. But if what your doing is taking a shroom ya bought from a dealer, literally smothered in contams, and was dried in a dehydrator to minimize the loss of actives, then no it won't work.


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Offlinedextr0
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Re: My intro... [Re: Pastywhyte]
    #23512237 - 08/05/16 10:07 AM (7 years, 5 months ago)

Let's get this straight...I have nothing to lose here and everything to gain. Truth is I have no idea how this mushroom was dried. My thought was, the stems have mycelial growth when dried. U break stem open its full of Cotton like mycelia. If I can get this to grow on agar or cardboard I will use that as spawn to inoculate my grain then move to monotub. Or at very least get me one spore print to work with. I understand the nessesity of sterilization and the nessesity of basically knowing for SURE your working with viable stuff. As in spores etc. Until I get spore print from FSRE I make do. It's also a cool experiment that will show others what may be possible for them.


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InvisiblePastywhyteMDiscord
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Re: My intro... [Re: dextr0]
    #23512268 - 08/05/16 10:21 AM (7 years, 5 months ago)

The issue is whether the cells are dead or not. Nothing to do with what it looks like when you snap it open. Not to mention the level of contamination you are dealing with. If you have nothing better to do with your time then go for it. But the odds are greatly stacked against you.

Truthfully I'm not even sure that I could pull that one off. Maybe if it was the last penis envy in existence I might try it. But I wouldn't hope for much.

Good luck


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Offlinedextr0
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Re: My intro... [Re: Pastywhyte]
    #23512371 - 08/05/16 10:55 AM (7 years, 5 months ago)

Thank u for the reply pasty. I have a question...in the third picture u can see mycelial growth. In your opinion is this what I'm looking for? It is not at all wispy it is very thick like a cotton ball.


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InvisiblePastywhyteMDiscord
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Re: My intro... [Re: dextr0]
    #23512528 - 08/05/16 11:42 AM (7 years, 5 months ago)

It's looking like mycelium. Of what species I cannot say. You might get really lucky. Ya never know. I'm just trying to temper your expectation somewhat and show you why we do things the way we do. Even if it is mycelium you certainly are going to have some bacteria riding along. I don't see how you will clean that up without agar. Maybe take it right to outdoors and hope for the the best. A bit of straw colonized with the cardboard then expanded to a small bed of straw and manure might work. Could maybe see a few grams like that.


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Offlinedextr0
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Re: My intro... [Re: Pastywhyte]
    #23512843 - 08/05/16 01:29 PM (7 years, 5 months ago)

My expectations are of course high as I'm the type of person to make things happen, especially in areas where I've taken good interest. I however know that odds are stacked against me. I am not advocating doing as I do, unless u are in circumstances similar to mine (broke but trying).
Cleaning the specimen I'm working with: this was addressed in RR's Tek that I am following. I have agar. I'm looking for a Tek that uses agar, honey, potato flakes, and brewers yeast. Problem is I just need to make a little so I'm working the math. I'm not good with math. With that said the Tek requires isolation of mycelia onto agar. Like you said the chances of contamination are high but with some work u should be able to isolate and eventually grow what you are looking for. Again on my part it will be luck and persistence if it does happen.


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InvisiblePastywhyteMDiscord
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Re: My intro... [Re: dextr0]
    #23512903 - 08/05/16 01:45 PM (7 years, 5 months ago)

Okay well if you have agar this might work. Standard agar recipes for fungi have a few basics.

Need between 1.5-2.5% agar. 1.5% agar will make it really soft and is good for germinating spores.

2.5% agar is very firm. Good for isolation work.

2% is the standard. Works for most stuff.

Nutes need to consist of a starch and a simple sugar. You want between 1-2% starch and 0-2% simple sugar. Do not use more sugar than starch. Stuff like peptide or yeast are not needed but sometimes beneficial.

So a recipe like
1000ml water
20 grams agar
20 grams potato flakes
10ml honey

Would work just fine. Scale according to need


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