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Rollin.n.Strollin



Registered: 07/25/16
Posts: 814
Loc: Australia
Last seen: 2 years, 2 months
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Rough Tek Method; un-finished.
#23511486 - 08/05/16 01:13 AM (7 years, 5 months ago) |
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So I've written up a method for my first PF tek method, it lacks a lot of content and requires adequate editing, but I'd like some conformation from everyone if I'm on the right path.
Cheers.
Pre requirements: Sterilized Equipment, Personal hygiene and sterile PPE.
1. - Environmental Sterilization; All doors and windows will be closed to prevent any air movement. Room will be sprayed to kill any airborne contaminants. Following the Television cabinet I'll be using as the "laboratory" will be sprayed and wiped down with isopropyl alcohol to again prevent contamination.
2. - Substrate mixing; I will begin to mix the substrate at a ratio of Verm:2 BRF:1 Water:1 in a large mixing bowl until combined, the substrate will be than placed lightly into the inoculation jars, which is then proceeded with a dry layer of Vermiculite per jar on top of the substrate.
3. - Inoculation substrate Sterilization; A large pot will be used as the PC replacement, which is filled roughly up to ?one third? of the height of the jars themselves. The pot will be slowly heated over a period of about 15 minutes to subtly heat the jars up, after the temp. will be increased to sterilize the jars. The jars will the be left to cool to room temperature.
4. - the first layer of tin foil on the jars will be removed, which is then proceeded by creating five holes in a star shape in each jar. Each jar will then be inoculated with the pre-sterilized syringe by putting the jar on a slight angle to promote the spore serum to run down each side of the jar.
5. - After each jar is inoculated, the sterile lids with the holes will then be tightened up on the jars. The jars will then be left in an incubation tub at temperatures of around 20-25 degree Celsius. Temperatures for the colonization period will be monitored to decrease fluctuation in temperatures.
6. - After the jars are completely colonized, the cakes will then be carefully removed from the jars and dunked for re-hydration of the cakes following colonization. The cakes will then be moved into a aquarium based terrarium, where temperature,Relative humidity and the cakes well-being will be monitored.
7. After each cake fruits to its maximum capability, fruits will be removed and dried out for storage. Following the success of the first flush a dunk and roll method will be used to re-hydrate the cakes, this will be repeated until the cakes capability of producing fruit is lost, and the cycle will be repeated with another generation of mushrooms.
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mindbentempire



Registered: 10/26/09
Posts: 258
Loc: Australia
Last seen: 17 days, 2 hours
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Some steps are incorrect, you are saying to make the holes in the lids after sterilizing? Holes should already be made prior to you even loading the jars up with substrate
There are so many posts already up on how to do PF TEK. Theres even videos by RogerRabbit. please, use those resources
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Adden

Registered: 06/04/03
Posts: 39,201
Loc:
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2. Make sure that after you load your jars - but before adding your dry verm barrier - use a small square of paper towel to wipe any excess BRF off the inner ring. It renders the DVB useless. Use a new square per jar so you're not rubbing BRF pieces from one jar to otherwise clean glass on the other. No one really mentions this and it seems to get looked over imo
5. No incubation tub
You also need a shotgun fruiting chamber.
You're going to goof. Start at square 1. Read the basics and watch RR's Let's Grow Mushrooms series on YouTube and come back to us with a fixed tek
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Mushierage
SWIM Sinker



Registered: 06/29/16
Posts: 1,094
Last seen: 7 years, 1 month
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Quote:
mindbentempire said: There are so many posts already up on how to do PF TEK. Theres even videos by RogerRabbit. please, use those resources
-------------------- Don't like researching posts? Read this! . Also, if you're new and your posts contain the words: Humidifer, incubator, air-stone, or heater, then you need to read and UTFSE before asking people to review your setup. OR... You should be cultivating reptiles and fish, not mushrooms.
  
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Adden

Registered: 06/04/03
Posts: 39,201
Loc:
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Re: Rough Tek Method; un-finished. [Re: Mushierage]
#23511679 - 08/05/16 04:42 AM (7 years, 5 months ago) |
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I think he's asking for input on how he's going to do it. Not rewriting the pftek, just asking for constructive criticism before he begins.
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Rollin.n.Strollin



Registered: 07/25/16
Posts: 814
Loc: Australia
Last seen: 2 years, 2 months
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Re: Rough Tek Method; un-finished. [Re: Adden]
#23511696 - 08/05/16 04:59 AM (7 years, 5 months ago) |
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Quote:
Adden said: I think he's asking for input on how he's going to do it. Not rewriting the pftek, just asking for constructive criticism before he begins.
I'm using other PF Teks as references to piece together my own written PF Tek, and as I said theres alot of missing content and it needs editing, just asking if theres anything in it that is incorrect or inneffective, thanks all
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Rollin.n.Strollin



Registered: 07/25/16
Posts: 814
Loc: Australia
Last seen: 2 years, 2 months
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Quote:
Rollin.n.Strollin said:
Quote:
Adden said: I think he's asking for input on how he's going to do it. Not rewriting the pftek, just asking for constructive criticism before he begins.
I'm using other PF Teks as references to piece together my own written PF Tek, and as I said theres alot of missing content and it needs editing, just asking if theres anything in it that is incorrect or inneffective, thanks all
To be more specific I just like to have my methods written in my own words, what I'm doing doesn't differ much from any other PF Tek its just easier for me to understand myself
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Mushierage
SWIM Sinker



Registered: 06/29/16
Posts: 1,094
Last seen: 7 years, 1 month
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Before publishing a written pf tek as you say, that is "pieced together from different teks" you should consider that the original tek that RR published gives you everything you need. Don't deviate from it.
Your tek is full of holes, and grammatical errors. It's full of missteps also, and not enough information which inevitably leads to failure due to contamination.
Stop trying to reinvent PFTek and just follow the original procedure. You'll have success of you don't deviate from it. It's that simple.
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mindbentempire



Registered: 10/26/09
Posts: 258
Loc: Australia
Last seen: 17 days, 2 hours
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Quote:
Rollin.n.Strollin said:
Quote:
Rollin.n.Strollin said:
Quote:
Adden said: I think he's asking for input on how he's going to do it. Not rewriting the pftek, just asking for constructive criticism before he begins.
I'm using other PF Teks as references to piece together my own written PF Tek, and as I said theres alot of missing content and it needs editing, just asking if theres anything in it that is incorrect or inneffective, thanks all
To be more specific I just like to have my methods written in my own words, what I'm doing doesn't differ much from any other PF Tek its just easier for me to understand myself:)
Your methods outlined so far are differing actually. And I'm sorry man, there's no 'missing content' when it comes to the PF Tek. What is it you are missing? Ask and we'll help
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Rollin.n.Strollin



Registered: 07/25/16
Posts: 814
Loc: Australia
Last seen: 2 years, 2 months
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Yeah it all makes sense, I'll just stick to an original PF method than, this is my first grow mind you so I'm not very intellect on the subject haha.
But Thanks for all the help much appreciated
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Musheadfred
Stranger


Registered: 07/23/16
Posts: 15
Last seen: 1 year, 9 months
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Hi the first image of inky cap looking mushies are they active???as I came across some and wasn't sure so let them go ,,,,so to speak, plus let me know ASAP cheers
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Rollin.n.Strollin



Registered: 07/25/16
Posts: 814
Loc: Australia
Last seen: 2 years, 2 months
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Re: Rough Tek Method; un-finished. [Re: Musheadfred]
#23511766 - 08/05/16 06:04 AM (7 years, 5 months ago) |
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No Mica caps aren't active, they resemble some actives such as liberty caps, but no they're not active, but they contain a chemical which I believe is call coprine, and it reacts with alcohol very bad.
Edited by Rollin.n.Strollin (08/05/16 06:05 AM)
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