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OfflineJollyrodger
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Mycelium from jar with wet spot
    #23510708 - 08/04/16 07:39 PM (7 years, 5 months ago)

This will be my third grow but this round ive been having trouble with bad spores. i managed to get 2 quarts colonized but both of them have a tiny bit of wet spot in the bottom that has been colonized around. I would like to try to get a few more jars out of them and want advice on the best approach.

I could grain to grain, i know the top 98% of the jar is clean. But im unsure of my g2g skills.

I was thinking i could take sterilized water maybe put a little peroxide in it and inject a jar, shake it up and take the mycelium water. I know i would get wet spot but atleast i shouldnt get mold because i didnt open the jar.

Wondering if it is possible. I dont feel i am in a good spot to get more spores right now and im not experienced with agar plates.

But i have made peroxidated agar in the past maybe that would be my best option. Bacteria cant grow on it but mycelium can.


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OfflineJollyrodger
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Re: Mycelium from jar with wet spot [Re: Jollyrodger]
    #23512092 - 08/05/16 09:12 AM (7 years, 5 months ago)

Im not in a good place to get more spores so i just want to try to get one good grow out of this. Wondering if anyone has ideas of the best way to use these jars to make more


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Invisiblemupetmower
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Re: Mycelium from jar with wet spot [Re: Jollyrodger]
    #23512106 - 08/05/16 09:19 AM (7 years, 5 months ago)

i feel like the only way you are going to have any luck at this point, using what you have, will involve using agar. the bacteria in that jar is probably meshed into the myc, and not just in the one spot on the bottom, and if you were to g2g, the resulting jars would likely also have bacteria..

i mean, im not an expert, so idk for sure. and hell, maybe it isnt enough bacteria to where it would completely fuck your grow or anything.

why not just start agar? it really isnt hard at all, and much easier than it seems at first. and its an excellent skill to have for this hobby. it can make one grain or drop of spores go really far, and clean.


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-The wise man never stops seeking knowledge.


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InvisibleJvF
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Re: Mycelium from jar with wet spot [Re: Jollyrodger]
    #23512112 - 08/05/16 09:20 AM (7 years, 5 months ago)

Depends in how much bacteria/pooling there is, i had this problem recently as well and my grow is just fine.

Definitely leave peroxide out of this.

Your best bet is agar, but it seems like you dont work with agar so here are some less succesful,yet plausible ways, to get this to work.


It may be possible to sterilize a spoon or similiar instrument and transfer a few grains from the top of the contaminated jar to a new jar under a SAB of flow hood.

Whatever you do dont shake the grains. Whatever i just told yoy relies solely on what you said about it ONLY being on the bottom.


Another option, which i just did last week, was use a spoon to get all the spawn out and then just leave an inch or so above where things start to get nasty. Then spawn that normally. It worked for me this lasy time :shrug:


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InvisiblePastywhyteMDiscord
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Re: Mycelium from jar with wet spot [Re: JvF] * 1
    #23512160 - 08/05/16 09:36 AM (7 years, 5 months ago)

Peroxide agar is really just agar. The heat of the PC turns the peroxide into water in minutes. Well before it even gets up to pressure.

If you must use spores to grain be sure to use very very little. Most spore syringes harbor at least a little bacteria so if you inject large amounts into the jar it pools and gives the bacteria a great place to bloom. A few drops is really all you need, there are thousands if not millions of spores in each drop. Technically you only need 2 to do a grow.

I would get a syringe from a reputable vendor, prep the grain carefully and inoculate 6 quarts with a few drops each. Then choose the best of the, and expand that with G2G. The other 5 can be spawned. If they contam at least you have the grain expanded from the good jar just around the corner.


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OfflineJollyrodger
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Re: Mycelium from jar with wet spot [Re: Pastywhyte]
    #23513213 - 08/05/16 03:22 PM (7 years, 5 months ago)

Right on, some good suggestions here. I do have some agar mix and some petri dishes. Id be willing to give it a go, why not. I just dont have a flow hood or glove box but i could make a still air box real quick.

Ill give it a shot and see what happens.


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OfflineJollyrodger
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Re: Mycelium from jar with wet spot [Re: Jollyrodger]
    #23513220 - 08/05/16 03:24 PM (7 years, 5 months ago)

I even have some dry golden teacher from my last one. Wonder if i could get anything off that.


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OfflineJollyrodger
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Re: Mycelium from jar with wet spot [Re: Jollyrodger]
    #23520454 - 08/07/16 09:35 PM (7 years, 5 months ago)

I ended up trying both grain to grain and i made some agar plates and transferred from 5he top of the jar. Just to see what happens.

I also had an old golden teacher plate that got dry so i put it on agar to see if itll rehydrate hahaha. I actually read that it can happen so itll be cool to watch.


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Offlineblackout
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Re: Mycelium from jar with wet spot [Re: Pastywhyte]
    #23521322 - 08/08/16 07:55 AM (7 years, 5 months ago)

Quote:

Pastywhyte said:
Peroxide agar is really just agar. The heat of the PC turns the peroxide into water in minutes.



The peroxide is added afterwards, just as its coming to pouring temp.

I wondered if peroxide might harbour contaminants not effected by the peroxide which could then grow in the agar mix, just like alcohol can be contaminated.


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InvisiblePastywhyteMDiscord
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Re: Mycelium from jar with wet spot [Re: blackout]
    #23521424 - 08/08/16 09:09 AM (7 years, 5 months ago)

Light will still break down the peroxide unless you pour it into opaque plates. Hence the brown bottle it comes in.


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OfflineJollyrodger
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Re: Mycelium from jar with wet spot [Re: Pastywhyte]
    #23522131 - 08/08/16 02:30 PM (7 years, 5 months ago)

Update:

So far both methods are looking real good, theyre showing great growth with no signs of contamination.


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