|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
ihatethis
Slightly Confused

Registered: 09/08/10
Posts: 521
|
New project / experiment: B+ / KSSS with BRF, WBS, LC, 50/50 Coir/Verm substrate
#23509263 - 08/04/16 11:41 AM (7 years, 5 months ago) |
|
|
I'm going to start by saying this has been a semi-hobby for me for around 6 years on and off. For the people that have criticized me in the past, I hope we are past that now as I feel like I know how to get a tub going 
Please partner with me and help me with feedback. Positive / negative - any feedback! I love it all. Thanks guys you are the reason most of us are still here.
I'll admit I went a bit crazy with this one. I had a project with a buddy that went sour and I now have the wheels turning in my head on how to get a good amount by September.
I give you my new 23 quart Presto pressure cooker and my appropriate Home Depot "Let's Do This" 5 Gal bucket:


I inoculate my jars by pre-heating the oven to the lowest temp and setting a pan down. I put my jars on this pan, put on gloves and alcohol soak the gloves after each inoculation. I then dip my syringe into alcohol and then flame sterilize it after each jar (not each inoculation).
Here are my inoculated 1/2 pint wide mouth Thailand Koh Samui Super Strain BRF jars, with 3 penny inch nail holes / micropore tape on lids. PC'ed at 12 PSI for 60 minutes. (9 cups verm, 4.5 cups BRF, 2.5 cups hot water with 5% gypsum diluted in it, 2 cups brewed coffee - twice used grounds for weaker coffee):

Here are my inoculated 1 quart wide mouth and 1 pint regular mouth Thailand Koh Samui Super Strain / B+ WBS jars, with 1/4 inch drilled holes / polyfill on lids. Tyvek under the lid, coffee filters on top. PC'ed at 15 PSI for 90 minutes (10 lbs WBS soaked for 24 hours in water with 5% gypsum diluted in it and 6 cups brewed coffee - twice used grounds for weaker coffee):


Here are my inoculated 1 pint regular mouth Thailand Koh Samui Super Strain liquid culture jars, with self sealing inoculation ports. PC'ed at 15 PSI for 15 minutes (1.5 cups hot water with 3 tsp of organic honey mixed in):

Here are my inoculated 1 quart wide mouth Thailand Koh Samui Super Strain BRF jars. These are made the same way. (Can you fine people help point me to a tek or help me get these to fruit *in the jar*?):

These are all sitting in a 70-80 degree climate:

Here is my substrate pasteurization technique 1/2 brick coir, 1 pint verm, 1 pint hot water with 5% gypsum diluted in it in oven bag with four micropore taped holes oven baked at 300 degrees until 175 internal temp reached then down to 190 degrees for 3 hours:


Teks used:
https://www.shroomery.org/8409/PF-Tek https://www.shroomery.org/9030/Doc34-s-Wild-Bird-Seed-Tek https://www.shroomery.org/forums/showflat.php/Number/17252080 https://www.shroomery.org/10477/Cybers-Liquid-Culture-Tek https://www.shroomery.org/forums/showflat.php/Number/15034407 https://www.shroomery.org/9666/Cybers-Self-Healing-Injection-point-lids https://www.shroomery.org/8393/Oven-Bag-Pasteurization-Pictorial
I also used many other random shroomery articles on Gypsum. The rest was improvisation to see what different results I can get!
Edited by ihatethis (08/04/16 12:07 PM)
|
amidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
|
Re: New project / experiment: B+ / KSSS with BRF, WBS, LC, 50/50 Coir/Verm substrate [Re: ihatethis]
#23509302 - 08/04/16 11:51 AM (7 years, 5 months ago) |
|
|
.
-------------------- The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.
Edited by amidogen (04/19/18 09:16 AM)
|
ihatethis
Slightly Confused

Registered: 09/08/10
Posts: 521
|
Re: New project / experiment: B+ / KSSS with BRF, WBS, LC, 50/50 Coir/Verm substrate [Re: amidogen]
#23509343 - 08/04/16 12:00 PM (7 years, 5 months ago) |
|
|
amidogen, thanks for reminding me to share how I inoculate. I have updated my post and here is what I updated:
I inoculate my jars by pre-heating the oven to the lowest temp and setting a pan down. I put my jars on this pan, put on gloves and alcohol soak the gloves after each inoculation. I then dip my syringe into alcohol and then flame sterilize it after each jar (not each inoculation).
Also, all jars were inoculated with the syringe, no agar. Although I was researching this last night but almost every tek I used this time around is new. I'm going to try agar in the future, if it really helps the amount of days it takes in total. I'm looking this time to get as much as fast as possible - while still allowing it to take it's *necessary* time.
Any advice helps here guys
Edited by ihatethis (08/04/16 12:12 PM)
|
amidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
|
Re: New project / experiment: B+ / KSSS with BRF, WBS, LC, 50/50 Coir/Verm substrate [Re: ihatethis]
#23509374 - 08/04/16 12:10 PM (7 years, 5 months ago) |
|
|
.
-------------------- The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.
Edited by amidogen (04/19/18 09:16 AM)
|
ihatethis
Slightly Confused

Registered: 09/08/10
Posts: 521
|
Re: New project / experiment: B+ / KSSS with BRF, WBS, LC, 50/50 Coir/Verm substrate [Re: amidogen]
#23509386 - 08/04/16 12:14 PM (7 years, 5 months ago) |
|
|
My method this time is different, only because of the fallout with my buddy. I used to use crumbled BRF cakes with a 50/50 verm / peat moss "casing" layer with no gypsum.
The oven is a "rigged" version of an inoculation chamber. It works because the hot air circulates around the top of the jars which forces any and all bacteria out of the area where I'm inoculating.
Edited by ihatethis (08/04/16 12:19 PM)
|
amidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
|
Re: New project / experiment: B+ / KSSS with BRF, WBS, LC, 50/50 Coir/Verm substrate [Re: ihatethis]
#23509440 - 08/04/16 12:33 PM (7 years, 5 months ago) |
|
|
.
-------------------- The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.
Edited by amidogen (04/19/18 09:16 AM)
|
ihatethis
Slightly Confused

Registered: 09/08/10
Posts: 521
|
Re: New project / experiment: B+ / KSSS with BRF, WBS, LC, 50/50 Coir/Verm substrate [Re: amidogen]
#23509825 - 08/04/16 02:49 PM (7 years, 5 months ago) |
|
|
Here are the B+ jars that made it out of the fallout - 1/2 pint WBS almost all fully colonized. My other buddy literally just brought them over this afternoon.

About to start the B+ mono with the pasteurized substrate in the earlier pictures - probably a medium size tub - around 20-30 quart maybe?
Edited by ihatethis (08/04/16 02:50 PM)
|
Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
|
Re: New project / experiment: B+ / KSSS with BRF, WBS, LC, 50/50 Coir/Verm substrate [Re: ihatethis]
#23509889 - 08/04/16 03:12 PM (7 years, 5 months ago) |
|
|
Quote:
mmmmmm said: I inoculate my jars by pre-heating the oven to the lowest temp and setting a pan down. I put my jars on this pan, put on gloves and alcohol soak the gloves after each inoculation. I then dip my syringe into alcohol and then flame sterilize it after each jar (not each inoculation).
What the fuck?
Seriously? This is how you inoculate? Please do not ever make any teks here.
|
Psykronik
OverGrower


Registered: 02/28/12
Posts: 150
Loc: Scotland
Last seen: 7 years, 4 months
|
Re: New project / experiment: B+ / KSSS with BRF, WBS, LC, 50/50 Coir/Verm substrate [Re: Inocuole]
#23509947 - 08/04/16 03:31 PM (7 years, 5 months ago) |
|
|
Quote:
Inocuole said:
Quote:
mmmmmm said: I inoculate my jars by pre-heating the oven to the lowest temp and setting a pan down. I put my jars on this pan, put on gloves and alcohol soak the gloves after each inoculation. I then dip my syringe into alcohol and then flame sterilize it after each jar (not each inoculation).
What the fuck?
Seriously? This is how you inoculate? Please do not ever make any teks here.
|
ByCoverOfNight
SirPsyOps
Registered: 07/21/16
Posts: 68
Loc: Arkham Asylum
Last seen: 7 years, 4 months
|
Re: New project / experiment: B+ / KSSS with BRF, WBS, LC, 50/50 Coir/Verm substrate [Re: ihatethis]
#23509948 - 08/04/16 03:31 PM (7 years, 5 months ago) |
|
|
I believe you forgot to consult the I Ching to confirm the right time to start your procedure, and to make certain the moon is full, the stars are aligned properly, the entrails of the sacrificed lamb concur, you've consulted the bone throwing shaman etc.. And you sadly omit wearing your hazmat suit, your oxygen re-breather or much less your lucky propeller cap. In short, your inoculation procedure is not nearly as tedious, boring or as beset with unnecessary complexities as any reasonable person would tell you it should be. One ( not me though ) should hope your sex life isn't nearly this inane.
|
ihatethis
Slightly Confused

Registered: 09/08/10
Posts: 521
|
Re: New project / experiment: B+ / KSSS with BRF, WBS, LC, 50/50 Coir/Verm substrate [Re: ByCoverOfNight]
#23510001 - 08/04/16 03:52 PM (7 years, 5 months ago) |
|
|
My last post was in 2014, and I got that inoculation tek from the guy that taught me how to grow. I promise you I'm not trying to post teks, only show how I grow. It works for me, so to each his own?
I have had 0 contaminated jars using this method. It's easy and doesn't require extra equipment.
Edited by ihatethis (08/04/16 03:54 PM)
|
Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
|
Re: New project / experiment: B+ / KSSS with BRF, WBS, LC, 50/50 Coir/Verm substrate [Re: ihatethis]
#23510009 - 08/04/16 03:55 PM (7 years, 5 months ago) |
|
|
That inoculation tek was bad 10 years ago. You can say it "works" for you and that it "doesn't require extra equipment" but all I use to inoculate is a plastic box, if that exceeds your budget for extra equipment I don't know what to tell you.
Let's see you do some agar using the oven tek.
|
mupetmower
Mower of Muppets



Registered: 03/29/16
Posts: 3,036
Loc: Here and There
|
Re: New project / experiment: B+ / KSSS with BRF, WBS, LC, 50/50 Coir/Verm substrate [Re: Inocuole]
#23510034 - 08/04/16 04:02 PM (7 years, 5 months ago) |
|
|
i always think it is kinda funny when people say ".. and i have had 0 contams doing it"... as if all of their stuff was completely free of any bacteria or other contams. like 100% clean... naw..
-------------------- -The wise man never stops seeking knowledge.
-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.
|
Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
|
Re: New project / experiment: B+ / KSSS with BRF, WBS, LC, 50/50 Coir/Verm substrate [Re: mupetmower]
#23510054 - 08/04/16 04:09 PM (7 years, 5 months ago) |
|
|
I just can't understand why a person wouldn't want to subject the methods they're going to use to ample peer review before using them, for years apparently.
|
Invertigo
Stranger

Registered: 03/30/16
Posts: 14
|
Re: New project / experiment: B+ / KSSS with BRF, WBS, LC, 50/50 Coir/Verm substrate [Re: Inocuole]
#23510169 - 08/04/16 04:55 PM (7 years, 5 months ago) |
|
|
Quote:
Psykronik said:
Quote:
Inocuole said:
Quote:
mmmmmm said: I inoculate my jars by pre-heating the oven to the lowest temp and setting a pan down. I put my jars on this pan, put on gloves and alcohol soak the gloves after each inoculation. I then dip my syringe into alcohol and then flame sterilize it after each jar (not each inoculation).
What the fuck?
Seriously? This is how you inoculate? Please do not ever make any teks here.

Quote:
Inocuole said: I just can't understand why a person wouldn't want to subject the methods they're going to use to ample peer review before using them, for years apparently.
Quote:
Inocuole said: That inoculation tek was bad 10 years ago. You can say it "works" for you and that it "doesn't require extra equipment" but all I use to inoculate is a plastic box, if that exceeds your budget for extra equipment I don't know what to tell you.
Let's see you do some agar using the oven tek.
To be fair, the "Oven Tek" was not necessarily meant for those who are working with agar. If you're doing agar work, chances are you're a bit more advanced, and have a better setup for your sterile work. I've seen the oven method used successfully, although it's obviously not ideal.
Point being, it's more geared towards those who are dipping their toes into the hobby. If the mycology bug bites, then one of the first things you work on is improving your sterile technique throughout your hobby.
|
Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
|
Re: New project / experiment: B+ / KSSS with BRF, WBS, LC, 50/50 Coir/Verm substrate [Re: Invertigo]
#23510256 - 08/04/16 05:23 PM (7 years, 5 months ago) |
|
|
But for the same amount of effort, you could not be using the oven tek, and be increasing your chances of success on the first try, without any actual experience.
|
mupetmower
Mower of Muppets



Registered: 03/29/16
Posts: 3,036
Loc: Here and There
|
Re: New project / experiment: B+ / KSSS with BRF, WBS, LC, 50/50 Coir/Verm substrate [Re: Inocuole]
#23510295 - 08/04/16 05:34 PM (7 years, 5 months ago) |
|
|
having done the oven door method on my first attempt, i can say with certainty that it is actually much less of a hassle to work in a SAB and requires much more effort to use the oven method...
dont have to be in front of a hot ass open oven, you have all of your stuff on a desk, with a decent work area, and not on a tray that can slip and move. and like inoc said, it increases your chances of success tenfold. plus, a tub for an SAB is 10 dollars max...
-------------------- -The wise man never stops seeking knowledge.
-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.
|
d0urd3n
Just call me "D"

Registered: 09/15/10
Posts: 5,237
|
Re: New project / experiment: B+ / KSSS with BRF, WBS, LC, 50/50 Coir/Verm substrate [Re: mupetmower]
#23510311 - 08/04/16 05:43 PM (7 years, 5 months ago) |
|
|
I'm more concerned about making LC with an MS syringe than using an oven rather than an SAB....
|
Invertigo
Stranger

Registered: 03/30/16
Posts: 14
|
Re: New project / experiment: B+ / KSSS with BRF, WBS, LC, 50/50 Coir/Verm substrate [Re: Inocuole]
#23510312 - 08/04/16 05:43 PM (7 years, 5 months ago) |
|
|
Agreed. There's a lot of info out there for folks to get lost in, and I know that there's a kit out there (non-vendor) that includes this inoc method as advice. I can't see many folks doing this more than once before realizing that there is a better way.
To that end, regardless of my opinion, "if it ain't broke, don't fix it". That would be one of the first things you should address if you are having contamination issues going forward though.
|
mupetmower
Mower of Muppets



Registered: 03/29/16
Posts: 3,036
Loc: Here and There
|
Re: New project / experiment: B+ / KSSS with BRF, WBS, LC, 50/50 Coir/Verm substrate [Re: Invertigo]
#23510558 - 08/04/16 07:03 PM (7 years, 5 months ago) |
|
|
they wouldnt ever admit it, even if they did have a contam issue.. hell, they've already said "I have had 0 contaminated jars using this method" which likely isnt true.. especially since they have been growing for as long as they have.
-------------------- -The wise man never stops seeking knowledge.
-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.
|
ihatethis
Slightly Confused

Registered: 09/08/10
Posts: 521
|
Re: New project / experiment: B+ / KSSS with BRF, WBS, LC, 50/50 Coir/Verm substrate [Re: mupetmower]
#23510880 - 08/04/16 08:23 PM (7 years, 5 months ago) |
|
|
Before this, I've only ever done BRF cakes and maybe that's why, to the best of my knowledge, I havent had a - sterilized inoculated jar - contaminated before using the oven inoculation tek? I've maybe done 40 BRF 1/2 pint jars tops this way in my days browsing shroomery.
So what I'm gathering from everyone's feedback is that for grains / LC an inoculation chamber is preferable, if not indeed necessary. I didn't know this; thanks for the tip guys! Money isn't an issue to buy the chamber - I just have been out of it for a while and I am very rusty.
|
Invertigo
Stranger

Registered: 03/30/16
Posts: 14
|
Re: New project / experiment: B+ / KSSS with BRF, WBS, LC, 50/50 Coir/Verm substrate [Re: ihatethis]
#23510987 - 08/04/16 09:01 PM (7 years, 5 months ago) |
|
|
LC from a multi-spore is not really recommended due to the heightened contamination rates. If you really want to use LC, the safest and cleanest way to do it is with agar. It's also been recommended to inoc a test jar up with the new LC, and seeing if there are any signs of contam before inoculating all your jars on a blind LC.
|
Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
|
Re: New project / experiment: B+ / KSSS with BRF, WBS, LC, 50/50 Coir/Verm substrate [Re: ihatethis]
#23511026 - 08/04/16 09:15 PM (7 years, 5 months ago) |
|
|
Quote:
mmmmmm said: Before this, I've only ever done BRF cakes and maybe that's why, to the best of my knowledge, I havent had a - sterilized inoculated jar - contaminated before using the oven inoculation tek? I've maybe done 40 BRF 1/2 pint jars tops this way in my days browsing shroomery.
So what I'm gathering from everyone's feedback is that for grains / LC an inoculation chamber is preferable, if not indeed necessary. I didn't know this; thanks for the tip guys! Money isn't an issue to buy the chamber - I just have been out of it for a while and I am very rusty.
Yep that's probably it. PF tek is designed in such a way that you can inoculate in open air if you do everything else just right and still get pretty good success.
|
|