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Invisibleihatethis
Slightly Confused

Registered: 09/08/10
Posts: 521
Re: New project / experiment: B+ / KSSS with BRF, WBS, LC, 50/50 Coir/Verm substrate [Re: mupetmower]
    #23510880 - 08/04/16 08:23 PM (7 years, 5 months ago)

Before this, I've only ever done BRF cakes and maybe that's why, to the best of my knowledge, I havent had a - sterilized inoculated jar - contaminated before using the oven inoculation tek? I've maybe done 40 BRF 1/2 pint jars tops this way in my days browsing shroomery.

So what I'm gathering from everyone's feedback is that for grains / LC an inoculation chamber is preferable, if not indeed necessary. I didn't know this; thanks for the tip guys! Money isn't an issue to buy the chamber - I just have been out of it for a while and I am very rusty.


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InvisibleInvertigo
Stranger

Registered: 03/30/16
Posts: 14
Re: New project / experiment: B+ / KSSS with BRF, WBS, LC, 50/50 Coir/Verm substrate [Re: ihatethis]
    #23510987 - 08/04/16 09:01 PM (7 years, 5 months ago)

LC from a multi-spore is not really recommended due to the heightened contamination rates.  If you really want to use LC, the safest and cleanest way to do it is with agar.  It's also been recommended to inoc a test jar up with the new LC, and seeing if there are any signs of contam before inoculating all your jars on a blind LC.


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InvisibleInocuole
Scalpel of Evil's Bane
 User Gallery

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Registered: 11/21/11
Posts: 24,863
Loc: ★
Trusted Cultivator
Re: New project / experiment: B+ / KSSS with BRF, WBS, LC, 50/50 Coir/Verm substrate [Re: ihatethis]
    #23511026 - 08/04/16 09:15 PM (7 years, 5 months ago)

Quote:

mmmmmm said:
Before this, I've only ever done BRF cakes and maybe that's why, to the best of my knowledge, I havent had a - sterilized inoculated jar - contaminated before using the oven inoculation tek? I've maybe done 40 BRF 1/2 pint jars tops this way in my days browsing shroomery.

So what I'm gathering from everyone's feedback is that for grains / LC an inoculation chamber is preferable, if not indeed necessary. I didn't know this; thanks for the tip guys! Money isn't an issue to buy the chamber - I just have been out of it for a while and I am very rusty.




Yep that's probably it.  PF tek is designed in such a way that you can inoculate in open air if you do everything else just right and still get pretty good success.


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