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OfflineRealmLAir
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Registered: 07/17/16
Posts: 94
Last seen: 2 years, 8 months
We Got Mycelium!!
    #23509018 - 08/04/16 10:18 AM (7 years, 5 months ago)

Good morning shroomers...

3 weeks ago (7/15) I inoculated 6 brf jars (didnt have a PC, and feel i might have made the substrate too moist) with a spore syringe. So far, no signs of growth. zero change from day one...Thats fine. im patient.

However, I also made a karo tek LC from the same syringe. I acquired myself a PC and on (8/2) I inoculated another 2 jars. I woke up today and there is definitely signs of mycelium growth.

I am going out of town for the weekend so Im quite anxious to return and see the progress. Im hoping the LC jar explodes by the time i get back.


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OfflineKillerclowns87
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Re: We Got Mycelium!! [Re: RealmLAir]
    #23509038 - 08/04/16 10:24 AM (7 years, 5 months ago)

Awesome im happy for you i hope things go well for you. I would think that your pf jars should deff be showing signs of mycelium growth after 3 weeks however. How many cc's did you use to innc. the jars?


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OfflinePsilicon
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Registered: 08/26/12
Posts: 7,057
Last seen: 3 years, 1 month
Re: We Got Mycelium!! [Re: Killerclowns87]
    #23509100 - 08/04/16 10:47 AM (7 years, 5 months ago)

A spore syringe LC is almost certainly nasty.  Do a test before you inoculate a batch with it.


--------------------
Agar - what, why and how?  Everything a beginner needs to know.
Oat Prep Tek
Bored?  Please take one of my experiments off my hands.


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InvisibleInocuole
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Re: We Got Mycelium!! [Re: Killerclowns87]
    #23509124 - 08/04/16 10:54 AM (7 years, 5 months ago)

Quote:

Killerclowns87 said:
Awesome im happy for you i hope things go well for you. I would think that your pf jars should deff be showing signs of mycelium growth after 3 weeks however. How many cc's did you use to innc. the jars?




You shouldn't be happy for someone that made LC with spores, they're about to have a very bad time in their future.

Quote:

RealmLAir said:
Im hoping the LC jar explodes by the time i get back.




Oh it will... It will.

:heytherebadboy:


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                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

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OfflineKillerclowns87
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Re: We Got Mycelium!! [Re: Inocuole]
    #23509260 - 08/04/16 11:41 AM (7 years, 5 months ago)

Quote:

Inocuole said:
Quote:

Killerclowns87 said:
Awesome im happy for you i hope things go well for you. I would think that your pf jars should deff be showing signs of mycelium growth after 3 weeks however. How many cc's did you use to innc. the jars?




You shouldn't be happy for someone that made LC with spores, they're about to have a very bad time in their future.



most likely yes


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Invisibleamidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
Re: We Got Mycelium!! [Re: RealmLAir]
    #23509279 - 08/04/16 11:45 AM (7 years, 5 months ago)

.


--------------------
The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.


Edited by amidogen (04/19/18 09:16 AM)


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OfflineRealmLAir
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Registered: 07/17/16
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Re: We Got Mycelium!! [Re: amidogen]
    #23520219 - 08/07/16 08:22 PM (7 years, 5 months ago)

Why am I about to have a bad time? I still have yet for someone to explain to me why spore syringe to LC is a bad idea...

Anyways, I just got back a few hours ago. Checked on my jars and this is from the Spore syringe LC

My other jar made from just a straight spore syringe is showing signs of progress, but not like this.


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InvisibleInvertigo
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Registered: 03/30/16
Posts: 14
Re: We Got Mycelium!! [Re: RealmLAir]
    #23520245 - 08/07/16 08:28 PM (7 years, 5 months ago)

LC is very nutrient dense, and the perfect medium for a variety of bacteria and goodies other than mycelium.  Spore syringes have a correlation of higher contamination rates.  This combination could cause your LC to produce either some great mycelium, or some great garbage.  Problem is, you probably won't be able to tell until you inoculate a jar/bag.  Agar work to isolate sterile mycelium is the most likely way to fire off a LC successfully.


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InvisibleInvertigo
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Re: We Got Mycelium!! [Re: Invertigo]
    #23520252 - 08/07/16 08:29 PM (7 years, 5 months ago)

No lid on your jar?  Glad to see mycelium at least...


Edited by Invertigo (08/07/16 08:35 PM)


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Invisibleamidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
Re: We Got Mycelium!! [Re: Invertigo]
    #23520391 - 08/07/16 09:12 PM (7 years, 5 months ago)

.


--------------------
The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.


Edited by amidogen (04/19/18 09:17 AM)


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OfflinePsilicon
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Registered: 08/26/12
Posts: 7,057
Last seen: 3 years, 1 month
Re: We Got Mycelium!! [Re: Invertigo]
    #23520399 - 08/07/16 09:15 PM (7 years, 5 months ago)

Spore syringes are almost always nasty.  When you put that nastiness into a liquid solution of sugar, the nutrients are brought right to every organism present in the solution by the process of diffusion.  The winners are the species which can consume and reproduce the fastest, and that's mold and bacteria.  They will pop up sooner or later.  Usually sooner.  If it's bacteria you can sometimes tell, but if it's mold you will be in for a very unpleasant surprise.

Since our big enemy, Trichoderma, is a parasitic mold, you can have a perfectly fine culture that tests well and is overcome during the period your test jar is growing, which will give you a nice set of green jars.  That isn't a guarantee, but I've seen it happen.


--------------------
Agar - what, why and how?  Everything a beginner needs to know.
Oat Prep Tek
Bored?  Please take one of my experiments off my hands.


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OfflineRealmLAir
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Registered: 07/17/16
Posts: 94
Last seen: 2 years, 8 months
Re: We Got Mycelium!! [Re: Invertigo]
    #23520410 - 08/07/16 09:17 PM (7 years, 5 months ago)

Yeah i was told by Mycologist (known poster on here) that as long as i had that dry verm barrier, the lid was unnecessary....Anybody have any opinions on that growth?

What should i do with my leftover CCs? PastyWhites Agar tek?


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InvisiblePsychedel.EXE
AKA Old Uncle Nutty
Male


Registered: 07/04/16
Posts: 211
Re: We Got Mycelium!! [Re: RealmLAir]
    #23520446 - 08/07/16 09:31 PM (7 years, 5 months ago)

Quote:

RealmLAir said:
What should i do with my leftover CCs? PastyWhites Agar tek?



^
Thats probably the best thing you could do with it.


--------------------



...


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OfflineMycologist217
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Re: We Got Mycelium!! [Re: Psychedel.EXE]
    #23520662 - 08/07/16 11:08 PM (7 years, 5 months ago)

I would never say a lid is unnecessary on a jar....if I did it was a joke, but I am certain I never talked about not needing a lid.


--------------------
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Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.


Edited by Mycologist217 (08/07/16 11:10 PM)


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OfflineKillerclowns87
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Registered: 06/11/16
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Loc: Florida
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Re: We Got Mycelium!! [Re: Mycologist217]
    #23521164 - 08/08/16 05:20 AM (7 years, 5 months ago)

Quote:

RealmLAir said:
Yeah i was told by Mycologist (known poster on here) that as long as i had that dry verm barrier, the lid was unnecessary....




Quote:

Mycologist217 said:
I would never say a lid is unnecessary on a jar....if I did it was a joke, but I am certain I never talked about not needing a lid.





BUUUSTED!!


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OfflineMushierage
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Registered: 06/29/16
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Re: We Got Mycelium!! [Re: Killerclowns87]
    #23521178 - 08/08/16 05:28 AM (7 years, 5 months ago)

Quote:

Killerclowns87 said:
Quote:

RealmLAir said:
Yeah i was told by Mycologist (known poster on here) that as long as i had that dry verm barrier, the lid was unnecessary....




Quote:

Mycologist217 said:
I would never say a lid is unnecessary on a jar....if I did it was a joke, but I am certain I never talked about not needing a lid.





BUUUSTED!!




Fuck outta here.... Lol.  I agree, m217 would never suggest this.  Only an idiot would suggest not having a lid on their jar.


--------------------
Don't like researching posts? Read this! . Also, if you're new and your posts contain the words: Humidifer, incubator, air-stone, or heater, then you need to read and UTFSE before asking people to review your setup.  OR... You should be cultivating reptiles and fish, not mushrooms.



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InvisibleInvertigo
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Registered: 03/30/16
Posts: 14
Re: We Got Mycelium!! [Re: RealmLAir]
    #23521593 - 08/08/16 10:17 AM (7 years, 5 months ago)

Quote:

RealmLAir said:

What should i do with my leftover CCs? PastyWhites Agar tek?




(I'm assuming CC's refers to your spore syringe) If you do that, you can isolate some clean myc for another go. You can even do agar with your leftover LC if you have any, and see if it's clean or nasty.

Remember to follow your chosen tek to the letter, and not freestyle.  As you progress in this hobby, the why's become very apparent for all of these little things, and that's when things start to get really fun (in my opinion).

If you don't have a good source of jars, you can use the same containers and lid design in PastyWhites Agar tek, for smaller BRF jars.


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