Home | Community | Message Board

Magic-Mushrooms-Shop.com
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Mushroom-Hut Liquid Cultures   North Spore Injection Grain Bag   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Original Sensible Seeds Bulk Cannabis Seeds   Left Coast Kratom Buy Kratom Extract

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Offlinenoppie
Stranger
 User Gallery

Registered: 06/05/09
Posts: 122
Last seen: 4 years, 3 months
PDA Agar ready to start kit
    #23508342 - 08/04/16 04:32 AM (7 years, 5 months ago)

Now i contacted with a lab they sell these PDA agar
Is it can be used with pan cyans?



Extras: Filter Print Post Top
OfflineMushierage
SWIM Sinker
 User Gallery


Registered: 06/29/16
Posts: 1,094
Last seen: 7 years, 1 month
Re: PDA Agar ready to start kit [Re: noppie]
    #23508348 - 08/04/16 04:40 AM (7 years, 5 months ago)

It can be used for growing any type of culture.


Extras: Filter Print Post Top
InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
 User Gallery
Folding@home Statistics
Registered: 04/30/13
Posts: 61,889
Loc: Milky way
Trusted Cultivator
Re: PDA Agar ready to start kit [Re: Mushierage]
    #23508400 - 08/04/16 05:16 AM (7 years, 5 months ago)

That's plate count not pda


Extras: Filter Print Post Top
OfflineMushierage
SWIM Sinker
 User Gallery


Registered: 06/29/16
Posts: 1,094
Last seen: 7 years, 1 month
Re: PDA Agar ready to start kit [Re: bodhisatta]
    #23508409 - 08/04/16 05:24 AM (7 years, 5 months ago)

Quote:

Trusted Cultivator said:
That's plate count not pda





Guess I shoulda read the fine print.


Extras: Filter Print Post Top
Offlinenoppie
Stranger
 User Gallery

Registered: 06/05/09
Posts: 122
Last seen: 4 years, 3 months
Re: PDA Agar ready to start kit [Re: Mushierage]
    #23508421 - 08/04/16 05:41 AM (7 years, 5 months ago)

This is PDA inside i think that label wrong.

Is that kit is ready to inoculate? Should i do/add anything? Just inoculate pan cyan spores inside? That's all?


Extras: Filter Print Post Top
Invisibleweetsie
unlicensed tub surgeon
 User Gallery


Registered: 05/08/11
Posts: 572
Loc: United Kingdom Flag
Re: PDA Agar ready to start kit [Re: noppie]
    #23508424 - 08/04/16 05:44 AM (7 years, 5 months ago)

If it's PDA then yes, transfer some spores to it.


--------------------
Active grow logs: Oysters on Straw Pellets
Trade list


Extras: Filter Print Post Top
OfflineMushierage
SWIM Sinker
 User Gallery


Registered: 06/29/16
Posts: 1,094
Last seen: 7 years, 1 month
Re: PDA Agar ready to start kit [Re: noppie]
    #23508435 - 08/04/16 05:51 AM (7 years, 5 months ago)

I would inoculate  in front of a flowhood or in a still air box.  If you are using MS solution make sure you flame sterilize the needle and inoculate away.

Good luck


Extras: Filter Print Post Top
Offlinespacechildo
proletarians rise up
Male User Gallery

Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
Re: PDA Agar ready to start kit [Re: Mushierage]
    #23509052 - 08/04/16 10:27 AM (7 years, 5 months ago)

it says plate count agar right on the label, why would it be PDA?
I wouldn't use that for mycology, its not what you want.


Extras: Filter Print Post Top
InvisibleMad Season
hookers and blackjack
 User Gallery


Registered: 09/16/12
Posts: 12,666
Loc: Canada Flag
Trusted Cultivator
Re: PDA Agar ready to start kit [Re: spacechildo]
    #23509101 - 08/04/16 10:47 AM (7 years, 5 months ago)



--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


Extras: Filter Print Post Top
InvisibleInocuole
Scalpel of Evil's Bane
 User Gallery

Folding@home Statistics
Registered: 11/21/11
Posts: 24,863
Loc: ★
Trusted Cultivator
Re: PDA Agar ready to start kit [Re: Mushierage] * 1
    #23509126 - 08/04/16 10:57 AM (7 years, 5 months ago)

Quote:

Mushierage said:
Quote:

Trusted Cultivator said:
That's plate count not pda





Guess I shoulda read the fine print.




Yeah the print is....  some of the biggest on the container.  So, not really fine at all.


--------------------
                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

Coir-ca-cola
Jokes are funny until they're about you


Extras: Filter Print Post Top
Offlinenoppie
Stranger
 User Gallery

Registered: 06/05/09
Posts: 122
Last seen: 4 years, 3 months
Re: PDA Agar ready to start kit [Re: Inocuole]
    #23510049 - 08/04/16 04:07 PM (7 years, 5 months ago)

Thank you guys i have double check by phonecall to company they told me inside is PDA inside (they prepare as i ordered it)

So anything i need more? Put pan cyan spore prints with syringe , colonize and get some mycelium with inoculation loop right?


Extras: Filter Print Post Top
Offlinespacechildo
proletarians rise up
Male User Gallery

Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
Re: PDA Agar ready to start kit [Re: noppie]
    #23510064 - 08/04/16 04:13 PM (7 years, 5 months ago)

swipe spores to plate with noc loop, transfer growth to new plates until you're sure its clean, let grow out and inoc grains with agar :thumbup:

are those ready poured dishes? they often come pre-contaminated in the mail, just a heads up.


Extras: Filter Print Post Top
Offlinenoppie
Stranger
 User Gallery

Registered: 06/05/09
Posts: 122
Last seen: 4 years, 3 months
Re: PDA Agar ready to start kit [Re: spacechildo]
    #23510152 - 08/04/16 04:45 PM (7 years, 5 months ago)

Some videos i watched are using syringe for that is it useless?
I am really confused and newbie your help appreciated

Yes petri dishes are pre-poured I don't think they contaminated with mail. At least i hope so.


Extras: Filter Print Post Top
Offlinespacechildo
proletarians rise up
Male User Gallery

Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
Re: PDA Agar ready to start kit [Re: noppie]
    #23510166 - 08/04/16 04:52 PM (7 years, 5 months ago)

OK so you have them petris already?
syringes work but you dont want too much water on the agar.
squirt some spore water on a flamed loop and streak on the plate, or use just 1 drop on the agar.
or just squirt out a drop or 2 so you "spill it" on the outside of your needle, and swipe that on the agar.
always flame the needle and loop before use of course. cool on the receiving agar plate if needed.


Extras: Filter Print Post Top
OfflineMushierage
SWIM Sinker
 User Gallery


Registered: 06/29/16
Posts: 1,094
Last seen: 7 years, 1 month
Re: PDA Agar ready to start kit [Re: Inocuole]
    #23510795 - 08/04/16 07:56 PM (7 years, 5 months ago)

Quote:

Inocuole said:
Quote:

Mushierage said:
Quote:

Trusted Cultivator said:
That's plate count not pda





Guess I shoulda read the fine print.




Yeah the print is....  some of the biggest on the container.  So, not really fine at all.





Point taken.  Though looking at it on a tiny IPhone screen, it looks pretty tiny to me. 


Anyway, OP, I'm curious as to why your plates would be mislabeled as PCA and not the proper PDA label, especially if it comes from a lab.  I just find that a little...weird.  You'd think that might be an important detail to a lab supply.  Just a mistake then?


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Mushroom-Hut Liquid Cultures   North Spore Injection Grain Bag   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Original Sensible Seeds Bulk Cannabis Seeds   Left Coast Kratom Buy Kratom Extract


Similar ThreadsPosterViewsRepliesLast post
* 9 different strains...starting multispore on agar.... Horseman 2,181 3 08/11/03 12:18 PM
by Twista
* agar with malted barley syrup Hongosmeester 7,034 17 10/27/19 10:42 PM
by ABCMORPHS
* sources of agar carnage 2,299 3 08/01/03 08:06 PM
by
* Pan cyans, on PDA?
( 1 2 all )
Akrobatik 2,630 22 04/21/07 03:57 PM
by strangladesh
* Re: Plate Count Agar? Is this ready to use? shroomeritus 2,048 8 04/24/01 07:53 PM
by shroomeritus
* Agar question? G a n j a 465 1 03/02/03 06:49 PM
by Prisoner#1
* Colonizing from Agar cookiewhore 1,594 7 01/28/02 02:29 PM
by cookiewhore
* Is this a fun kit for a starter??? ReeferJ 1,226 5 09/28/01 07:30 PM
by aluminum_can

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
524 topic views. 20 members, 233 guests and 34 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.026 seconds spending 0.008 seconds on 14 queries.