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Offlinenewtothis3
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sterile tek improvements?
    #23506806 - 08/03/16 06:19 PM (7 years, 5 months ago)

I use the PF tek as shown in the Let's Grow Mushrooms video posted on the site- have a verm layer on top in my jars, inoculate in a SAB, flame between jars, use micropore tape, etc.  I also tape over the holes again after inoculation.  Despite this, I still get a significant contamination rate.  I don't know what I am doing wrong.  Are those videos the best PF tek procedure?  Do you guys take any additional precautions or have any other suggestions to improve to avoid contamination?  Thank you!


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InvisibleInocuole
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Re: sterile tek improvements? [Re: newtothis3]
    #23506843 - 08/03/16 06:27 PM (7 years, 5 months ago)

If it's not the syringe it's probably the tape.  That's a whole lot of fucking around near the holes that you don't necessarily need to be doing, I'd definitely try to rule it out as a contam vector if it was me.


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OfflineMycologist217
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Re: sterile tek improvements? [Re: Inocuole]
    #23506916 - 08/03/16 06:45 PM (7 years, 5 months ago)

Are you using a pressure cooker for sterilization? Are you steaming? Are your contaminations appearing at inoculation points!

My first guess would also be the syringe, my second would be improper sterilization of the substrate.


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InvisibleComebackKid
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Re: sterile tek improvements? [Re: newtothis3]
    #23507174 - 08/03/16 07:44 PM (7 years, 5 months ago)

Only thing I can think of is that your sterilizing procedure must be off.

I've made syringes in very questionable conditions with success. A syringe from a vendor should be perfectly fine!

The only thing I've noticed is that (speaking from my homemade syringe experience) BRF jars that I PC'd for 60 minutes showed many signs of bacteria. Opposed to the jars that I PC'd for 90 minutes. 100% of my jars reached successful colonization.

Now the first time I ever tried PF tek, maybe 3 years ago, I didn't own a pressure cooker. The tek I was following told me to place my jars in a pot of boiling water halfway up the jars for an hour.
All my jars faced contamination.
In April I decided to follow that same tek again (which was a shitty tek btw) and gave it another go. This time I boiled the jar's for 2 hours just to be sure. I had 7/8 jars colonize.

It sounds like you are going overkill on your sterile procedure which to me seems like the only possibility is that your substrate isn't sterile to begin with.
I have not needed a SAB
I have never covered holes with MP tape

TLDR; Try pressure cooking your jars for at least 90 minutes


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Offlinenewtothis3
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Re: sterile tek improvements? [Re: Mycologist217]
    #23507860 - 08/03/16 11:03 PM (7 years, 5 months ago)

Yes I use a PC.  I don't see a pattern as to where the contamination emerges, though its mostly forest green mold.  What could be wrong with the micropore tape?  I used that because it was recommended in the videos. The syringes are from recommended vendors.


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Offlinenewtothis3
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Re: sterile tek improvements? [Re: newtothis3]
    #23507866 - 08/03/16 11:06 PM (7 years, 5 months ago)

I've been PCing at 15 PSI for 90 minutes, which is why I'm baffled.  I disinfect the SAB before working, too.


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Re: sterile tek improvements? [Re: newtothis3]
    #23508035 - 08/04/16 12:19 AM (7 years, 5 months ago)

Either your doing something bad like wiping the needle with alcohol after flaming or your syringes are fucked.


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InvisibleComebackKid
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Re: sterile tek improvements? [Re: Pastywhyte]
    #23508655 - 08/04/16 08:21 AM (7 years, 5 months ago)

Are you innoculating in the bathroom?
How old is your house? Maybe you have mold growing somewhere?


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:amanita2: Substrate surface conditions / Monotub prep and care :sporedrop:

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Re: sterile tek improvements? [Re: ComebackKid]
    #23508784 - 08/04/16 09:12 AM (7 years, 5 months ago)

I have mold growing in the beams above my SAB. Has no effect on my grow. A high mold contam rate with PF cakes is most likely  either poor inoculation practice or dirty syringes.


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Offlinenewtothis3
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Re: sterile tek improvements? [Re: ComebackKid] * 1
    #23509926 - 08/04/16 03:24 PM (7 years, 5 months ago)

I've inoculated in the bathroom twice and the problem worsened.  I disinfected it before of course.  The reason I did so was that I figured I could keep air flow to an absolute minimum, but have since read that is a bad spot to do this.  I've seen a video where a bathroom is suggested however.  My house is old, nearly 100.


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InvisibleInocuole
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Re: sterile tek improvements? [Re: newtothis3]
    #23509931 - 08/04/16 03:26 PM (7 years, 5 months ago)

Nobody in their right mind will recommend inoculating in a bathroom.  People who are doing stuff like that are the same people who probably tried the thing with the orange peels and the toothpaste in grade school.


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InvisibleComebackKid
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Re: sterile tek improvements? [Re: Inocuole]
    #23510011 - 08/04/16 03:56 PM (7 years, 5 months ago)

Lol what's this about orange peelz now?

And yeah Pasty, you gotta be right. Come to think of it, im sure my house is riddled with mold as well. Being over 100 yrs old and all.


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:amanita2: Substrate surface conditions / Monotub prep and care :sporedrop:

Look around you... Everything you see exists inside the mind.
Consciousness, the awareness that is experiencing this mind,
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Our individual experiences are all part of the universe's experience of itself


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InvisibleInocuole
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Re: sterile tek improvements? [Re: ComebackKid]
    #23510018 - 08/04/16 03:58 PM (7 years, 5 months ago)

You never heard that you could trip balls if you put toothpaste on some orange peels in a plastic bag and leave it out in the sun for a day, then eat it?  I remember hearing all kinds of stupid variations of that going through the public school system..


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InvisibleComebackKid
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Re: sterile tek improvements? [Re: Inocuole]
    #23510032 - 08/04/16 04:01 PM (7 years, 5 months ago)

Lmao never heard of that. Reminds me of jenkem though :lol:


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:amanita2: Substrate surface conditions / Monotub prep and care :sporedrop:

Look around you... Everything you see exists inside the mind.
Consciousness, the awareness that is experiencing this mind,
is peering in from outside the universe.

Our individual experiences are all part of the universe's experience of itself


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InvisibleInvertigo
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Re: sterile tek improvements? [Re: Inocuole]
    #23510202 - 08/04/16 05:06 PM (7 years, 5 months ago)

Quote:

Inocuole said:
You never heard that you could trip balls if you put toothpaste on some orange peels in a plastic bag and leave it out in the sun for a day, then eat it?  I remember hearing all kinds of stupid variations of that going through the public school system..




I remember this one... and also something vaguely about banana peels?  Here come the flashbacks!


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InvisibleInvertigo
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Re: sterile tek improvements? [Re: Invertigo]
    #23510232 - 08/04/16 05:16 PM (7 years, 5 months ago)

It may be some flaw in your SAB technique.  I've learned to let the box settle for a good 10-15 min after disinfecting everything in there with a generous spraying of lysol or other disinfectant.  Once you get your arms in the box, don't pull your arms out until you're done.  If you're constantly reaching in and out of the box, you're disturbing your "still air" (depending on the design of your box). 

Point is, be smooth and efficient, but not rushed. Re-sanitize and let settle again if you need to remove your arms or the still air concept becomes a moot point.  This has helped me see improved results at least.


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InvisibleAdden
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Re: sterile tek improvements? [Re: Inocuole]
    #23510358 - 08/04/16 06:01 PM (7 years, 5 months ago)

Quote:

Inocuole said:
You never heard that you could trip balls if you put toothpaste on some orange peels in a plastic bag and leave it out in the sun for a day, then eat it?  I remember hearing all kinds of stupid variations of that going through the public school system..




When I crack my neck, small amounts of acid get in my system and I trip. It's stored in your spine the rest of your life.


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InvisibleComebackKid
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Re: sterile tek improvements? [Re: Adden]
    #23510363 - 08/04/16 06:02 PM (7 years, 5 months ago)

Quote:

Adden said:
Quote:

Inocuole said:
You never heard that you could trip balls if you put toothpaste on some orange peels in a plastic bag and leave it out in the sun for a day, then eat it?  I remember hearing all kinds of stupid variations of that going through the public school system..




When I crack my neck, small amounts of acid get in my system and I trip. It's stored in your spine the rest of your life.



Speaking of that... anyone ever experience a flashback? Lol


--------------------
:amanita2: Substrate surface conditions / Monotub prep and care :sporedrop:

Look around you... Everything you see exists inside the mind.
Consciousness, the awareness that is experiencing this mind,
is peering in from outside the universe.

Our individual experiences are all part of the universe's experience of itself


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InvisibleAdden
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Re: sterile tek improvements? [Re: ComebackKid]
    #23510367 - 08/04/16 06:03 PM (7 years, 5 months ago)

Every time I smoke weed!


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InvisibleInvertigo
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Re: sterile tek improvements? [Re: Adden]
    #23510372 - 08/04/16 06:08 PM (7 years, 5 months ago)

Quote:

Adden said:
Quote:

Inocuole said:
You never heard that you could trip balls if you put toothpaste on some orange peels in a plastic bag and leave it out in the sun for a day, then eat it?  I remember hearing all kinds of stupid variations of that going through the public school system..




When I crack my neck, small amounts of acid get in my system and I trip. It's stored in your spine the rest of your life.




This one scared the hell out of me as a kid!  The name "Acid" implies toxicity to a youngster.


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