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Offlinealfonseelrick
Mycopath


Registered: 09/09/15
Posts: 606
Last seen: 1 year, 6 months
Need help in bacteria resistant agar
    #23505951 - 08/03/16 02:29 PM (7 years, 5 months ago)

hello just wondering if you guy ever had any luck in creating agar thats safe for spores but not for white slimy smelly bacteria i keep getting any info is greatly appreciated i will be conducting experiments either way mwahahaha


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InvisiblePsychedelic Pupil
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Re: Need help in bacteria resistant agar [Re: alfonseelrick]
    #23506055 - 08/03/16 02:56 PM (7 years, 5 months ago)

You could try an antibiotic agar (AA). There's a tek for one here:https://www.shroomery.org/forums/showflat.php/Number/17356799

You can also search for other recipes but you can add antibiotics to (I think) any agar recipe you're using


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Offlinealfonseelrick
Mycopath


Registered: 09/09/15
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Re: Need help in bacteria resistant agar [Re: Psychedelic Pupil]
    #23506695 - 08/03/16 05:56 PM (7 years, 5 months ago)

wow thank you bro that will be my starting approach!!


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Offline99.99
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Re: Need help in bacteria resistant agar [Re: alfonseelrick]
    #23506891 - 08/03/16 06:39 PM (7 years, 5 months ago)



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Offlinealfonseelrick
Mycopath


Registered: 09/09/15
Posts: 606
Last seen: 1 year, 6 months
Re: Need help in bacteria resistant agar [Re: 99.99]
    #23507247 - 08/03/16 08:03 PM (7 years, 5 months ago)

thanks you guys i really will have fun now!!!!


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Im just a fictional character everything stated by me is purely fictional and simply lies, those who like me are liars who where bribed or blackmailed in some way Muahahaha :stoned:


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Offlineanthiawe
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Re: Need help in bacteria resistant agar [Re: 99.99]
    #23510569 - 08/04/16 07:05 PM (7 years, 5 months ago)

Quote:

99.99 said:
I use this works great
http://www.fungi.com/product-detail/product/antibiotic-malt-extract-agar-12-pound.html




this exists!? what an age we live in :rocket:


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OfflineGreg
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Re: Need help in bacteria resistant agar [Re: anthiawe]
    #23511215 - 08/04/16 10:30 PM (7 years, 5 months ago)

Quote:

anthiawe said:
Quote:

99.99 said:
I use this works great
http://www.fungi.com/product-detail/product/antibiotic-malt-extract-agar-12-pound.html




this exists!? what an age we live in :rocket:




and to think I've been mixing gentamicin and MEA for all this time...


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Invisiblemicro
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Re: Need help in bacteria resistant agar [Re: Greg]
    #23511341 - 08/04/16 11:42 PM (7 years, 5 months ago)

gentamicin is nice because you can autoclave it


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Offlinealfonseelrick
Mycopath


Registered: 09/09/15
Posts: 606
Last seen: 1 year, 6 months
Re: Need help in bacteria resistant agar [Re: micro]
    #23511534 - 08/05/16 02:04 AM (7 years, 5 months ago)

awesome i was wondering how autoclavable prescription penecilin is ill sure to be looking into it you guys are all wonderful this is why i love this community so much!!! hahahaha:mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2::mushroom2:


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InvisiblebodhisattaMDiscordReddit
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Re: Need help in bacteria resistant agar [Re: alfonseelrick]
    #23513247 - 08/05/16 03:32 PM (7 years, 5 months ago)

Generally you want to not use antibacterial agar so that you can try to grab some mycelium and move it to a new dish avoiding the bacteria. If it grows you can see it. Antibacterial agar isn't fool proof you can bring bacteria along still and then it thrives when you move it to spawn. Use antibacterial agar to help with wild fruit spores/clones. But it certainly doesn't make up for techniques which 9 out of 10 times is the problem that antibacterial agar isn't the solution to


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Offlinealfonseelrick
Mycopath


Registered: 09/09/15
Posts: 606
Last seen: 1 year, 6 months
Re: Need help in bacteria resistant agar [Re: bodhisatta]
    #23514774 - 08/05/16 11:58 PM (7 years, 5 months ago)

woo thanks for the tips i just wanted to germinated some reishi spores not wild but i just ket getting this bacteria before they could grow :/ thanks guys :laugh: you rock!!! haha


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Im just a fictional character everything stated by me is purely fictional and simply lies, those who like me are liars who where bribed or blackmailed in some way Muahahaha :stoned:


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InvisiblebodhisattaMDiscordReddit
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Re: Need help in bacteria resistant agar [Re: alfonseelrick]
    #23515482 - 08/06/16 09:40 AM (7 years, 5 months ago)

Quote:

Trusted Cultivator said:
Quote:

Trusted Cultivator said:
Quote:

bodhisatta said:
Quote:

bodhisatta said:


take your spores to an inoculation loop. if you have a print then flame the loop dip in agar, pick spores off print and then swab the dish

if you have a syringe flame the loop and drop a few drops onto the loop to cool it down and then one more drop to load it with spores and then swab that








Quote:

bodhisatta said:
Try using an inoculation loop. Flame the syringe needle and the loop then squirt onto the loop to cool the needle and the loop. Squirt another drop to prime the loop. Then swab the dish.



Then you can see growth immediately, see contamination easier, grab healthy mycelium easier, and never worry about spore drops rolling all over a dish giving you germination everywhere at the same time.










also the agar link in my signature







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OfflinePsilocyBen17
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Re: Need help in bacteria resistant agar [Re: bodhisatta]
    #23516212 - 08/06/16 02:52 PM (7 years, 5 months ago)

Congrats on getting into agar OP! IMO its the most important part of this hobby.  I would recommend starting with regular agar. Pastywhites Easy Agar Tek is highly recommended:

https://www.shroomery.org/forums/showflat.php/Number/19208976

Pasty is the king of agar and most of what I know comes from the contributions he has made...

I hesitate to recommend the antibiotic stuff.  Antibiotic agar is really only for cloning exceptionally dirty fruits from the wild. It may work for your purposes, but If you use it all the time it will become a crutch preventing you from developing good technique.

If you do chose to go with antibiotic agar, make sure you transfer to regular agar before going to grains. This will ensure that the culture is actually clean and that you haven't just knocked the bacteria down enough to allow it to hitch a ride.

In the future you will get more replies if you post in Mushroom Cultivation....this isn't really an advanced topic.

Pb


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Edited by PsilocyBen17 (08/06/16 02:54 PM)


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Offlinealfonseelrick
Mycopath


Registered: 09/09/15
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Re: Need help in bacteria resistant agar [Re: PsilocyBen17]
    #23516413 - 08/06/16 04:32 PM (7 years, 5 months ago)

thanks ive been into agar since the beginning almost haha yea regular agar is easy and fun haha i was just trying to create a media that is more fungal friendly and not so friendly to bacteria ive got some dishes with a few drops of soap haha well see how that goes i dont think there will be any difference


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Im just a fictional character everything stated by me is purely fictional and simply lies, those who like me are liars who where bribed or blackmailed in some way Muahahaha :stoned:


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Offlinedrake89
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Re: Need help in bacteria resistant agar [Re: alfonseelrick]
    #23516442 - 08/06/16 04:41 PM (7 years, 5 months ago)

If you want to germinate reishi spore on agar include some fresh grouND reishi in your agar recipe.  Also try putting a living wedge on the opposite end of where you inoculate.  Sometimes that helps.


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InvisiblebodhisattaMDiscordReddit
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Re: Need help in bacteria resistant agar [Re: drake89]
    #23516450 - 08/06/16 04:43 PM (7 years, 5 months ago)

lucidium and resinacium germinate just fine on MEA and YPD


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Offlinealfonseelrick
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Posts: 606
Last seen: 1 year, 6 months
Re: Need help in bacteria resistant agar [Re: drake89]
    #23516967 - 08/06/16 08:16 PM (7 years, 5 months ago)

haha sorry dont have reishi nor any wedges :P thats y im trying to inoc but thanks


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Im just a fictional character everything stated by me is purely fictional and simply lies, those who like me are liars who where bribed or blackmailed in some way Muahahaha :stoned:


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