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Adden

Registered: 06/04/03
Posts: 39,201
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Re: Getting Grow Depression [Re: amidogen]
#23505683 - 08/03/16 01:27 PM (7 years, 5 months ago) |
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Hope this info below can help you OP. If you can, spring for a pressure cooker, it's like going fishing with a ball of string with no bait vs a new rod and reel.
My best guess is water getting into your dry verm barrier during steam sterilization.
1. Fill jar with 50/50 brf up to the ring.
2. Smooth out the inner rim and remove nutrition before adding the verm layer. I use the 3 section paper towels and rip them in half, set them on a dry surface, and use one per jar. Don't cross contam, you could be smearing BRF from jar 1 to jar 2.
3. PC at 15 psi for 45 minutes (when the rocker starts, so should your timer).
4. Let them sit overnight to cool. Never remove the rocker until you're ready to load your still air box.
5. Lay a towel down on a table. Spray it with soapy water. Clean out your SAB with 71% ISO, using paper towels not a rag, do it again with soapy water, spray all 5 sides with soapy water. Placing the two open holes against the towel.
6. Unload your PC and wipe the jars with 70% iso. Let sit 20 minutes or so.
6a. Wear gloves. Soak them in iso. (A face mask helps especially if you have facial hair. A shower doesn't hurt. Scrub your arms like you're going into surgery.)
7. Flame your needle outside your SAB, not near the arm holes. Do not wipe with alcohol; fire red hot will kill anything. Eject half a cc to cool the needle. Inject 1/4cc into each hole. Make sure your needle gets past the dry verm barrier.
8. Make sure you work mechanically with slow calculated movements.
9. Flame between jars, not each hole.
10. Use the right jars.
11. Try not to mess with them for two weeks. Trich and bacteria will show in 3 to 4 days, get those out of the house.
12. Make sure you don't leave your SAB to go grab something quick. If you have to leave your area, change your gloves when you come back.
13. Don't store contam jars near other projects.
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Adden

Registered: 06/04/03
Posts: 39,201
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Re: Getting Grow Depression [Re: Adden]
#23505706 - 08/03/16 01:32 PM (7 years, 5 months ago) |
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Took a look at your pics.. it appears so.. or your substrate was too wet and bacteria got in there. When the BRF/verm inside the jar looks like it's "cracked", and kind of comes loose, you've got a bacteria problem on your hands.
When you squeeze your verm/BRF you should get like one or two drops. Mix the water and verm first, then add a dusting of BRF and mix. Repeat this to prevent clumping.
..or just say fuck it, buy some Tupperware containers and put your spores on agar, clean it, put it on oats or rye and spawn to bulk.
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amidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
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Re: Getting Grow Depression [Re: Adden]
#23505775 - 08/03/16 01:46 PM (7 years, 5 months ago) |
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-------------------- The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.
Edited by amidogen (04/19/18 08:48 AM)
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Adden

Registered: 06/04/03
Posts: 39,201
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Re: Getting Grow Depression [Re: amidogen]
#23505795 - 08/03/16 01:52 PM (7 years, 5 months ago) |
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I'd let it ride and just let them do their thing at this point. Spores don't taste as bad as most make it out to be. A lot of people harvest earlier because they're prettier product.
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Just because I didn't take time to read the whole first post doesn't negate that I offered some good links and drew reasonable conclusions about the primary causes for concern. Honestly, it hardly matter what the OP's post says anymore in most threads, it almost always comes down to me posting the same handful of links over and over, because people usually need to all know the same shit, and they're damn good links. If the question can't be answered with a link I'll make a write up and then link them that.
Also did you just recommend Lysol?

I haven't owned a bottle of lysol in years. Not necessary at all. Got more contams when I DID use it, probably because it distracted from what the actual problems were.
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amidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
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Re: Getting Grow Depression [Re: Adden]
#23505833 - 08/03/16 02:01 PM (7 years, 5 months ago) |
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-------------------- The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.
Edited by amidogen (04/19/18 08:48 AM)
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The Mycologist
Explorer

Registered: 05/06/16
Posts: 3,024
Last seen: 30 days, 10 hours
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Re: Getting Grow Depression [Re: amidogen]
#23505845 - 08/03/16 02:04 PM (7 years, 5 months ago) |
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Yea the reason I asked is that without GE (gas exchange) the jars can stall out. Make sure the lids are on slightly loose if there are no other holes in the jars
-------------------- "That you are here—that life exists, and identity; That the powerful play goes on, and you will contribute a verse.” ― Walt Whitman, Leaves of Grass

Edited by The Mycologist (08/03/16 02:09 PM)
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Munchauzen


Registered: 06/22/11
Posts: 14,342
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Re: Getting Grow Depression [Re: Inocuole]
#23505856 - 08/03/16 02:05 PM (7 years, 5 months ago) |
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Working in a cloud of lysol is never advisable. GC's techniques are dated.
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Getting Grow Depression [Re: amidogen]
#23505860 - 08/03/16 02:06 PM (7 years, 5 months ago) |
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I wasn't trying to be rude, I just wanted some bullet points is all. The TNF recommended teks list is quality stuff though, no matter what the issue is, pretty sure you can find a solution there or in the basics thread. Most people here will line up to work with someone who actually wants to take measures to ensure success though. Too many "I'm gonna do what I want and then cry about the results and deny that anything was my fault" threads all over. Information is easy to come by if you're receptive to it.
Quote:
The Mycologist said: Yea the reason I asked is that without FAE (fresh air exchange) the jars can stall out. Make sure the lids are on slightly loose if there are no other holes in the jars
Jars do not require FAE, they require GE, which is not the same. GE needs adequate filtration, or a means by which contaminants cannot enter, while preventing a negative or positive pressure from forming. So, jar filters, or very slightly loosened plastic lids.
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Adden

Registered: 06/04/03
Posts: 39,201
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Re: Getting Grow Depression [Re: amidogen]
#23505907 - 08/03/16 02:17 PM (7 years, 5 months ago) |
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Quote:
amidogen said: I was more worried from reading that letting spores fall serves as a contam vector and signals the end of the life cycle to my cake.
With PF tek it's no big deal. It's when you're dealing with bulk that spore bombs become a reality. With certain strains (a misnomer for 'varieties'), people have said they get shittier flushes after spores drop (talking about dozens if not hundreds of mushrooms here). Off isolates or clones I don't know but people throw it around.
The end of a life cycle for gilled mushrooms happens when it runs out of nutrients. It approaches a stage called scenescens. That's when the mycelium goes into rest mode. At this stage, the organism has pushed out every last tiny little fruit it can, and when the patch is heavily spore laden and there's no point in reproducing, it goes back to feeding mode. Our tubs get discarded after a second or third flush, long before this happens.
We make Nature but better in cultivation. I think that's where the line gets blurred and people get confused. If you already have contaminated substrate, then a heavy spore load could kill a potential half flush or whatever else you could've squeezed out of it. I wouldn't worry about it until you get there.
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amidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
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Re: Getting Grow Depression [Re: Adden]
#23505921 - 08/03/16 02:22 PM (7 years, 5 months ago) |
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-------------------- The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.
Edited by amidogen (04/19/18 08:48 AM)
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d0urd3n
Just call me "D"

Registered: 09/15/10
Posts: 5,237
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Re: Getting Grow Depression [Re: amidogen]
#23505939 - 08/03/16 02:27 PM (7 years, 5 months ago) |
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Yeah +1 on no Lysol.
I like your attitude. Five shroom rating incoming.    
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Getting Grow Depression [Re: amidogen] 1
#23505941 - 08/03/16 02:27 PM (7 years, 5 months ago) |
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Quote:
amidogen said:
Quote:
Munchauzen said: Working in a cloud of lysol is never advisable. GC's techniques are dated.
Thanks for clearing that up. I honestly had doubts that lysol will kill airborne mold spores/bacteria effectively enough to make a difference. Besides, I'm working in an SAB from here on out. My understanding is that its virtue is trapping unwanted particles on the sides/floor rather than killing them.
We got a reader here folks!
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d0urd3n
Just call me "D"

Registered: 09/15/10
Posts: 5,237
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Re: Getting Grow Depression [Re: Inocuole]
#23505966 - 08/03/16 02:33 PM (7 years, 5 months ago) |
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george castanza
Lord Of The Idiots!


Registered: 10/21/02
Posts: 8,723
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Re: Getting Grow Depression [Re: Inocuole]
#23505967 - 08/03/16 02:33 PM (7 years, 5 months ago) |
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Ok kids. You spray the Lysol and then let it clean the air in your work area before you return. I don't recommend even staying in the room and breathing the Lysol if you can avoid it.
Yes, if you stand there and spray Lysol onto your work it will most certainly contaminate.
-------------------- KRAMER CAKES


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amidogen
see you on the other side

Registered: 05/07/16
Posts: 1,782
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Re: Getting Grow Depression [Re: Inocuole]
#23505989 - 08/03/16 02:38 PM (7 years, 5 months ago) |
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-------------------- The biggest trip of my life was realizing all of the events and actions described in posts made by this account were never real and had never actually happened, but were instead the delusional ramblings of a severely mentally ill human being. I just had to move on for my own good. I love you all.
Edited by amidogen (04/19/18 08:49 AM)
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george castanza
Lord Of The Idiots!


Registered: 10/21/02
Posts: 8,723
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Re: Getting Grow Depression [Re: amidogen]
#23505991 - 08/03/16 02:38 PM (7 years, 5 months ago) |
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Quote:
Munchauzen said: Working in a cloud of lysol is never advisable. GC's techniques are dated.
I see from your gallery I had better take your advice.(Sarcasm intended)
Don't buy into these guys that read other's post and then puke the information back up like they know something. Always trust your gut instincts and a tc tag if you can find one.
Ya that mushroom definitely beat the odds to be here. If I were going to eat it I would pasteurize it first.
-------------------- KRAMER CAKES


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d0urd3n
Just call me "D"

Registered: 09/15/10
Posts: 5,237
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Munch has some beautiful grows, what are you even talking about?
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george castanza
Lord Of The Idiots!


Registered: 10/21/02
Posts: 8,723
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Re: Getting Grow Depression [Re: d0urd3n]
#23506012 - 08/03/16 02:44 PM (7 years, 5 months ago) |
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Idk I just get tired of people throwing that dated shit around.
Lysol, oust both work great if used correctly.
-------------------- KRAMER CAKES


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Adden

Registered: 06/04/03
Posts: 39,201
Loc:
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Quote:
george castanza said: Ok kids. You spray the Lysol and then let it clean the air in your work area before you return. I don't recommend even staying in the room and breathing the Lysol if you can avoid it.
The mist that sinks down to the dirtiest 8 inches of a room isn't going to kick things up? Sounds unnecessary and plenty of people get along fine without wasting money.
That tard graemlin was uncalled for. I've seen you ban people for it. This is a place of learning. You should know that by now.
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