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Entheolaboratories
Stranger

Registered: 04/24/16
Posts: 4
Last seen: 5 years, 3 months
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My first Liquid Culture, stir every day or just before and after inoculation
#23504130 - 08/03/16 12:13 AM (7 years, 5 months ago) |
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Hello everybody, first of all thank you to be a member shroomery.org, and thank you to all the mushroom cultivation freaks out there for posting so much important info. Im on my first LC, I did BRF cakes yet and switched over to level 2 with WBS. My question is, if i should stir every day gently or just before and after inoculation, and if it looks like a normal growth yet as its orange/brown, instead of white what ive seen at other threads. It has build some kind of a clump on one of the jars. Ive used the creamcorn LC cloning tek using 435ml jars as well, filled ´em up with 200ml of destilled water and clean honey.
The first pic was made by cloning, two days after inoculation:
The second one was made by MSS, three days after inoculation:

Another thing to mention, the MSS was made by myself from the BRF cakes and I cannot say really if it was made contam free..
Successfull Cultivational Greetings Entheolaboratories
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Mycologist217
Frank's Disciple



Registered: 02/13/13
Posts: 2,425
Loc: Man of the Moon
Last seen: 1 day, 6 hours
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Re: My first Liquid Culture, stir every day or just before and after inoculation [Re: Entheolaboratories]
#23504148 - 08/03/16 12:19 AM (7 years, 5 months ago) |
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Clean cultures from Agar are really the only foolproof way to inoculate a Liquid culture, use a multistrain syringe to inoculate an agar plate, then use a clean agar wedge to inoculate the Lc...,it's one easy extra step that ensures clean culture.....but here are some pictures of water honey LC known to be clean:
 
Swirl it once or twice a day until you're done with it..,,
-------------------- My LC Manual (With custom LC lid Tek) ~~ Required Mycology Supplies ~~ Agar Work Videos ~~ L G M AMU Q&A-NO SYMPATHY FOR THE DEVIL! KEEP THAT IN MIND! BUY THE TICKET: TAKE THE RIDE Check out my Retail Gourmet Mushroom Farm! Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.
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Entheolaboratories
Stranger

Registered: 04/24/16
Posts: 4
Last seen: 5 years, 3 months
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Re: My first Liquid Culture, stir every day or just before and after inoculation [Re: Entheolaboratories]
#23504214 - 08/03/16 12:57 AM (7 years, 5 months ago) |
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Good thing ive Aga Agar laying here then, for emergency or just in case. I was also thinking about to buy a magnetic stir plate from ebay for like 38 bucks, i think it might be of benefit as ive just read of a faster colonization while constant swirl/stir, instead 7-10 days in just a few days.
Edited by Entheolaboratories (08/03/16 01:01 AM)
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: My first Liquid Culture, stir every day or just before and after inoculation [Re: Entheolaboratories]
#23504246 - 08/03/16 01:19 AM (7 years, 5 months ago) |
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Single cell organisms benefit from stir plate use but for mycelium low agitation or occasionally agitation is what you want. Break up the colony right before you use it
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Entheolaboratories
Stranger

Registered: 04/24/16
Posts: 4
Last seen: 5 years, 3 months
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Re: My first Liquid Culture, stir every day or just before and after inoculation [Re: bodhisatta]
#23508187 - 08/04/16 02:15 AM (7 years, 5 months ago) |
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Thank you everybody for the informations, much appreciated. Ill go through your signature Mycologist ;>
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