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OfflineLeeq
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Registered: 02/14/12
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Transplanting portion of colonized cake on to new grain
    #23502702 - 08/02/16 05:39 PM (7 years, 5 months ago)

Hey guys. I got my first flush off my first BRF cake. My mate stood on the cake accidentally and I think he's just stressed the mycelium a bit much,, as it isn't growing any more, even after dunking.

In a sterile environment, could I cut a portion of the mycelium off, and put it in to a sterile tub with some new fresh grain, full of nutrients? And harvest again? Cheers :smile:

Also what are some good grains I can use that I should be able to just by at the local supermarket?

I have a bag of dried rolled oats in the cupboard (For eating), would these work?


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Offlinelocallorax
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Re: Transplanting portion of colonized cake on to new grain [Re: Leeq]
    #23502756 - 08/02/16 05:53 PM (7 years, 5 months ago)

no you cannot. it is spent


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OfflineLeeq
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Re: Transplanting portion of colonized cake on to new grain [Re: locallorax]
    #23502789 - 08/02/16 06:04 PM (7 years, 5 months ago)

Quote:

locallorax said:
no you cannot. it is spent




Why will new, nutrient rich grains no allow it to continue?


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Offlinemindbentempire
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Re: Transplanting portion of colonized cake on to new grain [Re: Leeq]
    #23502939 - 08/02/16 06:43 PM (7 years, 5 months ago)

Quote:

Leeq said:
Quote:

locallorax said:
no you cannot. it is spent




Why will new, nutrient rich grains no allow it to continue?




Because it will become contaminant Central.


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InvisiblebodhisattaMDiscordReddit
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Re: Transplanting portion of colonized cake on to new grain [Re: mindbentempire]
    #23502942 - 08/02/16 06:44 PM (7 years, 5 months ago)

look up "super spawning"


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OfflineLeeq
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Re: Transplanting portion of colonized cake on to new grain [Re: mindbentempire]
    #23502975 - 08/02/16 06:52 PM (7 years, 5 months ago)

Quote:

mindbentempire said:
Quote:

Leeq said:
Quote:

locallorax said:
no you cannot. it is spent




Why will new, nutrient rich grains no allow it to continue?




Because it will become contaminant Central.



but surely if i sterilies the new grain, the new jar, and have an environment of clean air (Gas burns burning around me) and were to heat sterilise and alcohol sterilise all tools  involved with dioing it?


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InvisibleInocuole
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Re: Transplanting portion of colonized cake on to new grain [Re: Leeq]
    #23503266 - 08/02/16 08:19 PM (7 years, 5 months ago)

There are contaminants on the cake, so when the cake touches the grain, the contaminants will land on the grain.  These are.. you know.. pretty simple concepts here.

Mycelium that has already fruited will have lost most of its vigor anyway, will colonize very slowly and is significantly more likely to succumb to the bacteria which is surely present.  Just find a better way.  Agar could save you from doing all this.


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OfflineLeeq
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Re: Transplanting portion of colonized cake on to new grain [Re: Inocuole]
    #23504399 - 08/03/16 03:46 AM (7 years, 5 months ago)

Fir, cheers for the info guys. For the sake of my learning i'm gonna try it anyhow


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Invisibleweetsie
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Re: Transplanting portion of colonized cake on to new grain [Re: Leeq]
    #23504445 - 08/03/16 04:41 AM (7 years, 5 months ago)

Quote:

Leeq said:
In a sterile environment, could I cut a portion of the mycelium off, and put it in to a sterile tub with some new fresh grain, full of nutrients? And harvest again? Cheers :smile:

I have a bag of dried rolled oats in the cupboard (For eating), would these work?




How are you planning on sterilizing this tub and keeping it sterile? No one does uncolonized grain in a tub, it's completely unfeasible. If you were to transfer it to grain (using a proper sterile grain tek) I would take a really small piece from the middle of the cake to increase the odds of success. Break it instead of cutting as that will just push contaminants from the outside in.

Rolled oats are also no good, you don't want starchy grain as it would just turn into a bacterial mess.

Agar can be done for less than $10, I'd highly recommend you look into that instead and read up on sterile grain prep.


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OfflineLeeq
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Registered: 02/14/12
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Re: Transplanting portion of colonized cake on to new grain [Re: weetsie]
    #23506193 - 08/03/16 03:39 PM (7 years, 5 months ago)

Quote:

weetsie said:
Quote:

Leeq said:
In a sterile environment, could I cut a portion of the mycelium off, and put it in to a sterile tub with some new fresh grain, full of nutrients? And harvest again? Cheers :smile:

I have a bag of dried rolled oats in the cupboard (For eating), would these work?




How are you planning on sterilizing this tub and keeping it sterile? No one does uncolonized grain in a tub, it's completely unfeasible. If you were to transfer it to grain (using a proper sterile grain tek) I would take a really small piece from the middle of the cake to increase the odds of success. Break it instead of cutting as that will just push contaminants from the outside in.

Rolled oats are also no good, you don't want starchy grain as it would just turn into a bacterial mess.

Agar can be done for less than $10, I'd highly recommend you look into that instead and read up on sterile grain prep.




Well I'm buggered then, was amphed-up and wanted to give it a go.
Don't worry! I'm in the process of a proper grow, no winging it, doing it properly.


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OfflineLeeq
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Registered: 02/14/12
Posts: 64
Last seen: 7 years, 2 months
Re: Transplanting portion of colonized cake on to new grain [Re: Leeq]
    #23506247 - 08/03/16 03:59 PM (7 years, 5 months ago)

Also, sterilising. Alcohol rubbed the all sides, had alcohol burners going around me, wore surgical gloves. Like I said was on a fair bit of speed and just couldn't help trying.


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