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Leeq
Stranger

Registered: 02/14/12
Posts: 64
Last seen: 7 years, 2 months
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Transplanting portion of colonized cake on to new grain
#23502702 - 08/02/16 05:39 PM (7 years, 5 months ago) |
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Hey guys. I got my first flush off my first BRF cake. My mate stood on the cake accidentally and I think he's just stressed the mycelium a bit much,, as it isn't growing any more, even after dunking.
In a sterile environment, could I cut a portion of the mycelium off, and put it in to a sterile tub with some new fresh grain, full of nutrients? And harvest again? Cheers 
Also what are some good grains I can use that I should be able to just by at the local supermarket?
I have a bag of dried rolled oats in the cupboard (For eating), would these work?
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locallorax
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Registered: 07/27/12
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Re: Transplanting portion of colonized cake on to new grain [Re: Leeq]
#23502756 - 08/02/16 05:53 PM (7 years, 5 months ago) |
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no you cannot. it is spent
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Leeq
Stranger

Registered: 02/14/12
Posts: 64
Last seen: 7 years, 2 months
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Re: Transplanting portion of colonized cake on to new grain [Re: locallorax]
#23502789 - 08/02/16 06:04 PM (7 years, 5 months ago) |
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Quote:
locallorax said: no you cannot. it is spent
Why will new, nutrient rich grains no allow it to continue?
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mindbentempire



Registered: 10/26/09
Posts: 258
Loc: Australia
Last seen: 16 days, 22 hours
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Re: Transplanting portion of colonized cake on to new grain [Re: Leeq]
#23502939 - 08/02/16 06:43 PM (7 years, 5 months ago) |
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Quote:
Leeq said:
Quote:
locallorax said: no you cannot. it is spent
Why will new, nutrient rich grains no allow it to continue?
Because it will become contaminant Central.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Transplanting portion of colonized cake on to new grain [Re: mindbentempire]
#23502942 - 08/02/16 06:44 PM (7 years, 5 months ago) |
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look up "super spawning"
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Leeq
Stranger

Registered: 02/14/12
Posts: 64
Last seen: 7 years, 2 months
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Re: Transplanting portion of colonized cake on to new grain [Re: mindbentempire]
#23502975 - 08/02/16 06:52 PM (7 years, 5 months ago) |
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Quote:
mindbentempire said:
Quote:
Leeq said:
Quote:
locallorax said: no you cannot. it is spent
Why will new, nutrient rich grains no allow it to continue?
Because it will become contaminant Central.
but surely if i sterilies the new grain, the new jar, and have an environment of clean air (Gas burns burning around me) and were to heat sterilise and alcohol sterilise all tools involved with dioing it?
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Transplanting portion of colonized cake on to new grain [Re: Leeq]
#23503266 - 08/02/16 08:19 PM (7 years, 5 months ago) |
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There are contaminants on the cake, so when the cake touches the grain, the contaminants will land on the grain. These are.. you know.. pretty simple concepts here.
Mycelium that has already fruited will have lost most of its vigor anyway, will colonize very slowly and is significantly more likely to succumb to the bacteria which is surely present. Just find a better way. Agar could save you from doing all this.
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Leeq
Stranger

Registered: 02/14/12
Posts: 64
Last seen: 7 years, 2 months
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Re: Transplanting portion of colonized cake on to new grain [Re: Inocuole]
#23504399 - 08/03/16 03:46 AM (7 years, 5 months ago) |
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Fir, cheers for the info guys. For the sake of my learning i'm gonna try it anyhow
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weetsie
unlicensed tub surgeon



Registered: 05/08/11
Posts: 572
Loc: United Kingdom
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Re: Transplanting portion of colonized cake on to new grain [Re: Leeq]
#23504445 - 08/03/16 04:41 AM (7 years, 5 months ago) |
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Quote:
Leeq said: In a sterile environment, could I cut a portion of the mycelium off, and put it in to a sterile tub with some new fresh grain, full of nutrients? And harvest again? Cheers 
I have a bag of dried rolled oats in the cupboard (For eating), would these work?
How are you planning on sterilizing this tub and keeping it sterile? No one does uncolonized grain in a tub, it's completely unfeasible. If you were to transfer it to grain (using a proper sterile grain tek) I would take a really small piece from the middle of the cake to increase the odds of success. Break it instead of cutting as that will just push contaminants from the outside in.
Rolled oats are also no good, you don't want starchy grain as it would just turn into a bacterial mess.
Agar can be done for less than $10, I'd highly recommend you look into that instead and read up on sterile grain prep.
-------------------- Active grow logs: Oysters on Straw Pellets Trade list
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Leeq
Stranger

Registered: 02/14/12
Posts: 64
Last seen: 7 years, 2 months
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Re: Transplanting portion of colonized cake on to new grain [Re: weetsie]
#23506193 - 08/03/16 03:39 PM (7 years, 5 months ago) |
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Quote:
weetsie said:
Quote:
Leeq said: In a sterile environment, could I cut a portion of the mycelium off, and put it in to a sterile tub with some new fresh grain, full of nutrients? And harvest again? Cheers 
I have a bag of dried rolled oats in the cupboard (For eating), would these work?
How are you planning on sterilizing this tub and keeping it sterile? No one does uncolonized grain in a tub, it's completely unfeasible. If you were to transfer it to grain (using a proper sterile grain tek) I would take a really small piece from the middle of the cake to increase the odds of success. Break it instead of cutting as that will just push contaminants from the outside in.
Rolled oats are also no good, you don't want starchy grain as it would just turn into a bacterial mess.
Agar can be done for less than $10, I'd highly recommend you look into that instead and read up on sterile grain prep.
Well I'm buggered then, was amphed-up and wanted to give it a go. Don't worry! I'm in the process of a proper grow, no winging it, doing it properly.
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Leeq
Stranger

Registered: 02/14/12
Posts: 64
Last seen: 7 years, 2 months
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Re: Transplanting portion of colonized cake on to new grain [Re: Leeq]
#23506247 - 08/03/16 03:59 PM (7 years, 5 months ago) |
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Also, sterilising. Alcohol rubbed the all sides, had alcohol burners going around me, wore surgical gloves. Like I said was on a fair bit of speed and just couldn't help trying.
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