Home | Community | Message Board

MushroomMan Mycology
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Left Coast Kratom Buy Kratom Extract   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Mushroom-Hut Substrate Bags   Kraken Kratom Red Vein Kratom

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Offlinedonkeycong
Dreamer


Registered: 05/05/14
Posts: 46
Last seen: 3 years, 3 months
No growth on agar!?
    #23501630 - 08/02/16 12:00 PM (7 years, 5 months ago)

I made pasty plates 3 days ago and it said in the tek that growth should be seen by the 3rd day. My plates have ZERO growth. Print used is a year old stored in drawer. Should I be concerned?


Extras: Filter Print Post Top
InvisibleMad Season
hookers and blackjack
 User Gallery


Registered: 09/16/12
Posts: 12,666
Loc: Canada Flag
Trusted Cultivator
Re: No growth on agar!? [Re: donkeycong]
    #23501676 - 08/02/16 12:13 PM (7 years, 5 months ago)

No. Be worried after 2 weeks or something.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


Extras: Filter Print Post Top
OfflinePsilocyBen17
Pin Pornographer


Registered: 10/20/13
Posts: 3,751
Last seen: 1 year, 1 month
Re: No growth on agar!? [Re: Mad Season]
    #23501697 - 08/02/16 12:17 PM (7 years, 5 months ago)

Is the wedge you transfered atleast fuzzing up? the last batch of agar I used was a new brand called Seaweed Solutions. It was supposed to be PDA but there wasn't enough nutes in it to promote myc growth...

no growth usually=not enough nutes.

I had to start over. I added a bunch of honey to the next batch. Just did the transferees now, hoping they work, it sucks putting work in to be taking steps backwards.


--------------------
PsilocyBen's Trade List!


Extras: Filter Print Post Top
InvisibleInocuole
Scalpel of Evil's Bane
 User Gallery

Folding@home Statistics
Registered: 11/21/11
Posts: 24,863
Loc: ★
Trusted Cultivator
Re: No growth on agar!? [Re: PsilocyBen17]
    #23501718 - 08/02/16 12:24 PM (7 years, 5 months ago)

Quote:

PsilocyBen17 said:
Is the wedge you transfered atleast fuzzing up? the last batch of agar I used was a new brand called Seaweed Solutions. It was supposed to be PDA but there wasn't enough nutes in it to promote myc growth...

no growth usually=not enough nutes.

I had to start over. I added a bunch of honey to the next batch. Just did the transferees now, hoping they work, it sucks putting work in to be taking steps backwards.




What wedge?  This is spores to agar.


--------------------
                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

Coir-ca-cola
Jokes are funny until they're about you


Extras: Filter Print Post Top
OfflinePsykronik
OverGrower
 User Gallery


Registered: 02/28/12
Posts: 150
Loc: Scotland Flag
Last seen: 7 years, 4 months
Re: No growth on agar!? [Re: Inocuole]
    #23501726 - 08/02/16 12:28 PM (7 years, 5 months ago)

My MS agar inoculation took around 7-8 days to show signs of growth.


Extras: Filter Print Post Top
OfflinePsilocyBen17
Pin Pornographer


Registered: 10/20/13
Posts: 3,751
Last seen: 1 year, 1 month
Re: No growth on agar!? [Re: Inocuole]
    #23501747 - 08/02/16 12:37 PM (7 years, 5 months ago)

Quote:

Inocuole said:
Quote:

PsilocyBen17 said:
Is the wedge you transfered atleast fuzzing up? the last batch of agar I used was a new brand called Seaweed Solutions. It was supposed to be PDA but there wasn't enough nutes in it to promote myc growth...

no growth usually=not enough nutes.

I had to start over. I added a bunch of honey to the next batch. Just did the transferees now, hoping they work, it sucks putting work in to be taking steps backwards.




What wedge?  This is spores to agar.




My bad, didnt read the OP very closely. I do that sometimes when the same questions get asked over and over. If they are spores keep waiting. If there is no growth after a week or two its probably because your agar is too firm.


--------------------
PsilocyBen's Trade List!


Extras: Filter Print Post Top
Offlinedonkeycong
Dreamer


Registered: 05/05/14
Posts: 46
Last seen: 3 years, 3 months
Re: No growth on agar!? [Re: donkeycong]
    #23501864 - 08/02/16 01:10 PM (7 years, 5 months ago)

Ok cool I feel better now!


Extras: Filter Print Post Top
InvisiblePastywhyteMDiscord
Say hello to my little friend
Male User Gallery


Registered: 09/15/12
Posts: 37,810
Loc: Canada Flag
Trusted Cultivator
Re: No growth on agar!? [Re: donkeycong]
    #23502289 - 08/02/16 03:31 PM (7 years, 5 months ago)

Quote:

donkeycong said:
I made pasty plates 3 days ago and it said in the tek that growth should be seen by the 3rd day. My plates have ZERO growth. Print used is a year old stored in drawer. Should I be concerned?




I said growth from spores should be seen in the third day? I don't remember saying that.


Extras: Filter Print Post Top
InvisibleInocuole
Scalpel of Evil's Bane
 User Gallery

Folding@home Statistics
Registered: 11/21/11
Posts: 24,863
Loc: ★
Trusted Cultivator
Re: No growth on agar!? [Re: Pastywhyte]
    #23502589 - 08/02/16 05:06 PM (7 years, 5 months ago)

^ Me either.


--------------------
                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

Coir-ca-cola
Jokes are funny until they're about you


Extras: Filter Print Post Top
Offlinedonkeycong
Dreamer


Registered: 05/05/14
Posts: 46
Last seen: 3 years, 3 months
Re: No growth on agar!? [Re: Pastywhyte]
    #23504760 - 08/03/16 08:38 AM (7 years, 5 months ago)

I re-read and I got that idea from elasticaltiger's Tiger Drop tek (https://www.shroomery.org/forums/showflat.php/Number/20111637) where he discusses how he inoculates the plates under "The Move" step

"Your dishes are now inoculated. You should see growth in about 3 days. Contamination should be easily visible.  I discard contaminated agar and wash the plates immediately since I have no intention of using them."


Extras: Filter Print Post Top
InvisiblePastywhyteMDiscord
Say hello to my little friend
Male User Gallery


Registered: 09/15/12
Posts: 37,810
Loc: Canada Flag
Trusted Cultivator
Re: No growth on agar!? [Re: donkeycong]
    #23504772 - 08/03/16 08:42 AM (7 years, 5 months ago)

I think the reason he said that is he is suggesting that you are using a fresh print that you took yourself. But really spores can take up to 3 weeks or longer to germ.


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Left Coast Kratom Buy Kratom Extract   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Mushroom-Hut Substrate Bags   Kraken Kratom Red Vein Kratom


Similar ThreadsPosterViewsRepliesLast post
* Pins on agar and one with contaminant TripPSmittens 1,690 10 05/07/21 09:45 AM
by TripPSmittens
* How to make agar 2 agar transfer? esp 6,656 7 01/08/04 11:08 AM
by YidakiMan
* transferring agar meatman 2,485 3 10/02/02 01:13 PM
by tripndicular
* Transfering live mycelium to stagnant PF jar Viking 995 7 03/31/10 09:04 PM
by Doc_T
* using agar? downforpot 2,213 6 12/02/01 07:00 PM
by Anno
* Agar questions-I may have goofed blahblahblah 3,283 6 01/11/02 02:58 PM
by ar393
* Re: Agar w/ h202, any info?? 3Mshroom 2,151 8 05/08/00 04:25 AM
by mycofile
* Question about agar nd595 3,920 18 03/10/04 04:35 PM
by Anonymous

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
1,224 topic views. 13 members, 137 guests and 32 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.023 seconds spending 0.007 seconds on 12 queries.